Genetic diversity of Indonesian cauliflower cultivars and their relationships with hybrid cultivars grown in Australia     

Ida A. Astarini  Julie A. Plummer  Rachel A. Lancaster  Guijun Yan 《Scientia Horticulturae》2006

The objectives of this study were to investigate genetic variation and relationships between Indonesia-, Australian- and European-based cultivars and to evaluate variation within Indonesia cultivars as all cultivars are open-pollinated. Eight cauliflower cultivars collected from three production regions in Indonesia and four F₁ hybrids cultivars grown in Australia were evaluated using RAPD and ISSR markers. DNA polymorphisms generated from 10 polymorphic RAPD primers were used to construct a dendogram using the unweighted pair-group method with arithmetic averages (UPGMA) and to generate a fingerprinting key. ISSR marker analysis using 14 primers were attempted but DNA polymorphisms could not be clearly identified. The RAPD technique indicated that variation occurred both within and between Indonesian cultivars. Comparison between Indonesian-, Australian- and European-based cultivars showed that Indonesian cultivars have unique genotypes and would be good sources of genes for future crop improvement.  相似文献   

Plasma pepsinogen levels in some experimental infections of Ostertagia ostertagi in cattle   总被引：1，自引：0，他引：1  

J F Michel  M B Lancaster  C Hong  S Berrett 《The Veterinary record》1978,103(17):370-373

Two groups of calves were infected with larvae of Ostertagia ostertagi to establish large numbers of adults and arrested larvae. In one group symptoms of ostertagiasis were seen and there was a loss of three months growth; in the other, in which adult worms were removed by a single anthelmintic treatment, there was only a transient reduction in live-weight gain. Plasma pepsinogen levels were however the same in the two groups and followed the same course. Even after 25 weeks, when calves had been growing normally for up to three months, plasma pepsinogen values were still around 5 iu per litre, well above the level generally regarded as diagnostic of ostertagiasis. The relevance of these findings to the use of the test in the diagnosis of ostertagiasis is discussed. The literature is reviewed.  相似文献   

Comparison of ELISA and CFT assays for Chlamydophila abortus antibodies in ovine sera     

McCauley LM  Lancaster MJ  Young P  Butler KL  Ainsworth CG 《Australian veterinary journal》2007,85(8):325-328

OBJECTIVE: To compare the sensitivity and specificity of Chlamydophila abortus antibody assays, to find a suitable serological assay for testing sheep for export. DESIGN: Comparison of results from known positive and negative sheep populations. PROCEDURE: Fifty-five positive and fifty negative sera were analysed by four enzyme linked immunosorbent assays (ELISA), three using recombinant antigens based on the chlamydial polymorphic outer membrane proteins (POMP90-3, POMP90-4, POMP80-90) and one using a synthetic peptide based on chlamydial major outer membrane proteins (MOMP-P). They were also analysed by complement fixation tests (CFT) using crude antigens from chlamydia isolated from an Australian sheep, a Californian parakeet and a Texan turkey. Assay sensitivity and specificity were expressed as point estimates and 95% confidence intervals. Results were compared using McNemar's test for paired samples. RESULTS: ELISA sensitivity ranged from 70 to 98% and complement fixation test sensitivity from 60 to 96%; with POMP90-3 > POMP90-4 > CFT (parakeet) > CFT (turkey) > POMP80-90 > MOMP-P > CFT (sheep). There was no significant difference from POMP90-3 to POMP80-90 (P > 0.05). ELISA specificity ranged from 88 to 100% and CFT specificity was 100% for all three antigens; with CFT and POMP90-4 > MOMP-P > POMP80-90 > POMP90-3. There was no significant difference from CFT to POMP80-90 (P > 0.05). Changing the CFT cut-off from 1:32 to 1:4 substantially reduced the specificity with little improvement in sensitivity. CONCLUSION: Assays using POMP90-4, POMP80-90, CFT (parakeet) and CFT (turkey) had equivalent sensitivity and specificity; none of the ELISAs were more specific than any CFT. The POMP80-90 ELISA is recommended as an alternative to CFT (parakeet) but as its specificity is not ideal the search for a more specific assay should continue.  相似文献   

Mycotoxic nervous disease in cattle fed sprouted barley contaminated with Aspergillus clavatus     

El-Hage CM  Lancaster MJ 《Australian veterinary journal》2004,82(10):639-641

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Soil microbial activity is affected by Roundup WeatherMax and pesticides applied to cotton (Gossypium hirsutum)     

Lancaster SH  Haney RL  Senseman SA  Hons FM  Chandler JM 《Journal of agricultural and food chemistry》2006,54(19):7221-7226

Adoption of glyphosate-based weed control systems has led to increased use of the herbicide with continued use of additional pesticides. Combinations of pesticides may affect soil microbial activity differently than pesticides applied alone. Research was conducted to evaluate the influence of glyphosate-based cotton pest management systems on soil microbial activity. Soil was treated with commercial formulations of trifluralin, aldicarb, and mefenoxam + pentachloronitrobenzene (PCNB) with or without glyphosate (applied as Roundup WeatherMax). The soil microbial activity was measured by quantifying C and N mineralization. Soil microbial biomass was determined using the chloroform fumigation-incubation method. Soils treated with glyphosate alone exhibited greater cumulative C mineralization 30 days after treatment than all other treatments, which were similar to the untreated control. The addition of Roundup WeatherMax reduced C mineralization in soils treated with fluometuron, aldicarb, or mefenoxam + PCNB formulations. These results indicate that glyphosate-based herbicides alter the soil microbial response to other pesticides.  相似文献   

2011 Alpharma Beef Cattle Nutrition Symposium:enhancing beef production efficiency with new knowledge and technologies: building the bridges for future collaboration     

DL Boss  MF Allan  KA Johnson  PA Lancaster  AE Wertz-Lutz  ME Branine 《Journal of animal science》2012,90(7):2299-2300

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Planetary science. Linear dunes on Titan     

Lancaster N 《Science (New York, N.Y.)》2006,312(5774):702-703

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Acceleration of uranium at the bevalac     

Alonso JR  Avery RT  Elioff T  Force RJ  Grunder HA  Lancaster HD  Lofgren EJ  Meneghetti JR  Selph FB  Stevenson RR  Yourd RB 《Science (New York, N.Y.)》1982,217(4565):1135-1137

Recent upgrading projects have extended the mass range of particles that can be accelerated at the Bevalac to include any element of the periodic table to energies above 1 billion electron volts per atomic mass unit. This capability was verified on 11 May 1982 with the production of a uranium beam at 147.7 million electron volts per atomic mass unit.  相似文献   

Nitrite inhibition of Clostridium botulinum: electron spin resonance detection of iron-nitric oxide complexes   总被引：10，自引：0，他引：10  

D Reddy  J R Lancaster  D P Cornforth 《Science (New York, N.Y.)》1983,221(4612):769-770

Vegetative cells of Clostridium botulinum were shown to contain iron-sulfur proteins that react with added nitrite to form iron-nitric oxide complexes, with resultant destruction of the iron-sulfur cluster. Inactivation of iron-sulfur enzymes (especially ferredoxin) by binding of nitric oxide would almost certainly inhibit growth, and thus is probably the mechanism of botulinal inhibition by nitrite in foods.  相似文献   

Studies on inflammation in the sheep, using turpentine-induced pleurisy as a test system. 1. Studies on the pleural exudates     

J L Vegad  M C Lancaster 《New Zealand veterinary journal》1972,20(12):232-237

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