湖南省果梅品种资源种质杂化状况的初步研究   总被引：8，自引：0，他引：8  

刘连森  贺善文 《园艺学报》1993,20(3):225-230

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水平连续铸钢凝固过程数值模拟     

刘金水  蒋冰 《湖南农业大学学报(自然科学版)》1995,22(3)

本文在分析水平连铸传热特征的基础上，给出了一维情况下，计算结晶器出口处铸坯凝固层厚度的解析式，建立了计及轴向传热的连续坯凝固模拟模型。进行了连续坯二维传热的凝固数值模拟，其模拟结果与实验结果吻合较好，具有普遍应用价值。  相似文献   

仙客来离体叶培养与植株再生   总被引：8，自引：0，他引：8  

张喜春  刘宏伟 《东北林业大学学报》1992,20(4):18-21

以仙客来叶片、叶柄及其不同部位为外植体进行诱导,结果幼叶基部(叶子基部与叶柄相连处的断口部分)诱导愈伤组织频率最高,可达100％。愈伤组织在N_6+BA2mg/1+2,4-D0.5mg/1培养基上培养,约40d左右可100％分化出不定芽,其不定芽数量一块愈伤组织最多可分化出30个以上。将幼苗移入1/2MS+NAA 0.5mg/1培养基中生根最好。  相似文献   

Analysis and pharmacokinetics of cimaterol in growing Holstein steers.     

T M Byrem  T F Robinson  Y R Boisclair  A W Bell  W S Schwark  D H Beermann 《Journal of animal science》1992,70(12):3812-3819

Pharmacokinetic parameters for the beta 2-adrenergic agonist, cimaterol (CIM), were determined in growing Holstein steers. Compartmental analysis was used after measurement of CIM in body fluids by affinity chromatography and HPLC using UV detection. Recoveries from spiked plasma and urine standards were 70 +/- 1.2% and 68 +/- 1.1%, respectively. The minimum detection level in plasma was 1 ng/mL and the average CV was 5.1% for concentrations that ranged from 1 to 30 ng/mL. Four steers (276 +/- 24 kg) received 15 mg of CIM by bolus intravenous injection. Plasma CIM levels declined in a biphasic manner with half-lives of 2.5 min for the distribution phase and 54 min for the elimination phase. A two-compartment open model was used to describe the disappearance of CIM and the following pharmacokinetic parameters were obtained: central compartment volume (Vc) = .76 L/kg, apparent volume of distribution (Vd) = 4.1 L/kg, and transfer rate constants from the central to peripheral compartment (k12) = .177/min, from the peripheral to central compartment (k21) = .054/min and elimination from the central compartment (kel) = .074/min. After 8 h, total urinary CIM accounted for only 18.3% of the administered dose. Results suggest that circulating concentrations of CIM in growing steers are influenced by its accumulation in an unidentified peripheral pool and its conversion into unknown metabolite(s) before elimination.  相似文献   

On the genetic basis of equine allergic diseases: II. Insect bite dermal hypersensitivity.     

E Marti  H Gerber  S Lazary 《Equine veterinary journal》1992,24(2):113-117

The horses studied were of the Swiss Warmblood breed and most were ELA-typed to assess a possible association of dermal hypersensitivity to insect bites with the major histocompatibility complex. Firstly, the occurrence of the condition was examined in 304 half-siblings sired by six stallions (A to F). Fourteen cases of dermal hypersensitivity were recognized and all were in the 153 offspring of Stallions C, E and F. Most animals of this group were also investigated for chronic hypersensitivity bronchitis: none of the sires displayed clinical signs of dermal hypersensitivity, but Stallions D, E and F were affected by chronic bronchitis. Among the animals investigated for both conditions only one horse showed coincidence of the two diseases as can be expected when the diseases are not correlated. The frequency of manifest dermal hypersensitivity and/or chronic hypersensitivity bronchitis varied in the half-sibling groups of individual sires. These findings suggest that the allergic conditions are independent entities. Secondly, the occurrence of dermal hypersensitivity was studied in three generations of horses at a stud at which Stallion C had exerted a particularly strong influence. A total of 302 animals, all born and raised at this stud, were surveyed over a period of 12 years. The descendants of Stallion C showed a significantly higher incidence (P less than 0.01) of dermal hypersensitivity (two daughters out of 19; eight second generation offspring out of 103; one third generation offspring out of 85) than did the controls of the same age classes but unrelated to Stallion C at the same stud (0 out of 95).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

A seeker of the truth.     

H A Waters 《Journal of the American Veterinary Medical Association》1992,201(11):1689-1690

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冬季不供热简易产蛋鸡舍的设计与应用研究     

刘文生  仇雪梅 《黑龙江八一农垦大学学报》1992,(1):105-111

通过实验表明:在冬季最低气温-28.3℃的黑龙江省密山市饲养产蛋鸡,只要鸡舍保温良好,适当增加鸡的饲养密度,不用供热,也可保持鸡舍内温度在13℃—15℃的最佳鸡产蛋环境温度;在151天的冬季里实验组鸡,按入舍鸡计算平均每只鸡产蛋103.8个,产蛋率为68.74%,仅比对供热取暖鸡舍的对照组鸡群产蛋率低0.5%,故在寒冷的黑龙江省推广产蛋鸡舍冬季不供热是完全可能的,其经济效益也是显著的。  相似文献   

Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.          下载免费PDF全文

K F Lawson  H Chiu  M Matson  P Bachmann    J B Campbell 《Canadian journal of veterinary research》1992,56(2):135-141

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.  相似文献   

Behavioral and physiological effects of freeze or hot-iron branding on crossbred cattle.     

D C Lay  T H Friend  R D Randel  C L Bowers  K K Grissom  O C Jenkins 《Journal of animal science》1992,70(2):330-336

Twenty-seven crossbred calves (1/2 Simmental, 1/4 Hereford, 1/4 Brahman) averaging 257 +/- 11 d of age were either hot-iron-branded (H), freeze-branded (F), or sham-branded (S). Calves were blocked for temperament, weight, and sex and were randomly assigned to day and order in which treatments were applied. To reduce stress from handling at treatment time, each calf was herded through the squeeze chute daily for 5 d before the experiment. Jugular cannulas were inserted in each calf 1 d before application of treatment. Blood samples and heart rate measures were obtained at -5, -3, 0, .5, 1, 3, 5, 10, 15, and 20 min after application of the treatments. Mean concentrations of plasma epinephrine (EPI) were higher for H calves at time .5 min than for either S or F calves (P = .10). To account for individual differences, prebranding heart rates and hormone concentrations were subtracted from subsequent samples and were also used to calculate a proportion for each subsequent sample. Analyses of subtracted values found that EPI concentrations were greater for H calves than for either S or F calves (P = .007) at .5 min postbranding. No other differences were found for the subtracted analyses. Analyses of proportion data also revealed that H calves had greater EPI than did either S or F calves (P = .027) at .5 min postbranding. Only three animals vocalized during branding, one H calf and two F calves. Despite the 5-d acclimation period, handling and restraint elevated plasma cortisol concentrations and heart rate.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Occurrence of Staphylococcus intermedius on the hair and skin of normal dogs.     

R P Allaker  D H Lloyd  A I Simpson 《Research in veterinary science》1992,52(2):174-176

Aerobic bacterial populations were studied on the distal hair coat and at the skin surface of the shoulder, rump and abdomen of 10 healthy dogs. Coagulase negative staphylococci (CNS) were more frequently isolated from the hair than the skin at the shoulder and rump. There was no difference in the isolation rate of coagulase positive staphylococci (CPS) (Staphylococcus intermedius) between the hair and skin. Total skin counts were greatest on the abdomen whereas CNS counts from the hair were least at this site. There were no differences between CPS counts at the three sites on either hair or skin. The populations on the relatively unfavourable microenvironment of the distal hair may represent contamination rather than colonisation. The low populations of CPS at the skin surface also indicate contamination or transient colonisation rather than true resident status.  相似文献