Clinical experiences with halothane inhalation anesthesia in sheep     

A Orság  J Tkác 《Veterinární medicína》1971,16(10):603-612

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水稻白叶枯病发生流行预测的研究     

何明  罗显芝  张成琬 《植物病理学报》1982,12(4):22-26

 作者应用多重回归法和噬菌体技术对水稻白叶枯病的发生流行趋势的预测作了研究。结果表明:(1)根据始病前两旬降水量≥ 0.1毫米日数和越冬期(12月至2月)三个月的日平均气温,可以预测田间始病期,(2)在水稻生育期内测定稻田水中噬菌体量,并根据病害流行前两旬连续三次测定噬菌体累计量、降水量和降水量≥ 10毫米日数等可预测水稻后期病害的流行趋势。同时分析了影响病害流行的主导因素(降水量≥ 10毫米日数和噬菌体量)及建立了多重回归预测模式。  相似文献   

Vitamin E levels in the ovine serum after administration of vitamin E and selenium]     

G Kovác  L Vrzgula 《Veterinární medicína》1978,23(1):1-7

The effects of drugs containing vitamin E for peroral administration (Combinal E), intramuscular injection (Erevit) and parenteral administration of a combined drug containing vitamin E and selenium (Selevit) on vitamin E levels in the ovine blood serum were studied. A uniform dose of 15 mg tocopherol acetate per 1 kg live weight was administered to the experimental animals. The level of vitamin E after the peroral administration of Combinal E was affected to a lesser extent than in swine and calves. The serum was examined before and after saponification to determine the effect of the intramuscular injection of Erevit; it was found out that tocopherol acetate prevailed in the blood serum in the first hours after such a route of administration. The best results were obtained after both subcutaneous and intramuscular administration of Selevit, the levels of free tocopherol showing high values even at the end of the experiments. These results may be made use of in determining effective preventive and therapeutic measures to do away with the white muscle disease in practice.  相似文献   

Comparison of selected diagnostic methods for identification of Cryptosporidium parvum and Cryptosporidium andersoni in routine examination of faeces     

Kvác M  Kvetonová D  Půzová G  Ditrich O 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(8):405-411

This study involved the comparison of suitability of different methods for routine diagnostics of Cryptosporidium spp. Two staining methods, one concentration-sedimentation method, seven concentration-floatation methods and one combined floatation-sedimentation method were compared. The methods were tested with two concentrations (1 x 10(5) and 1 x 10(6)/g) of C. parvum and C. andersoni. The methods were evaluated using light microscope, magnification 400x for concentration methods and 1000x for stained samples respectively. Specificity of both staining methods was 95-100%. Ziehl-Neelsen with P < 0.01 is more suitable for identification of C. andersoni and modified Milácek-Vítovec with P < 0.01 for identification of C. parvum. Concerning specificity and sensitivity, the floatation-concentration method by Sheather was found to provide the best results of all selected methods. The merthiolate iodine formaldehyde concentration (MIFC) method was the least specific one. The least suitable method concerning sensitivity and costs was the floatation method with caesium chloride (CsCl) with a specificity of 29%.  相似文献   

Characterization of the Exoglucanase-Encoding Gene PaEXG2 and Study of Its Role in the Biocontrol Activity of Pichia anomala Strain K     

Grevesse C  Lepoivre P  Jijakli MH 《Phytopathology》2003,93(9):1145-1152

ABSTRACT The PaEXG2 gene, encoding an exo-beta-1,3-glucanase, was isolated from the biocontrol agent Pichia anomala strain K. PaEXG2 has the capacity for coding an acidic protein of 427 amino acids with a predicted molecular weight of 45.7 kDa, a calculated pI of 4.7, and one potential N-glycosylation site. PaEXG2 was disrupted by the insertion of the URA3 marker gene, encoding orotidine monophosphate decarboxylase in strain KU1, a uracil auxotroph derived from strain K. Strain KU1 showed inferior biocontrol activity and colonization of wounds on apples, compared to the prototrophic strain. Antagonism and colonization were recovered after the restoration of prototrophy by transformation with the URA3 gene. Integrative transformation was shown to be mostly ectopic in strain K descendants (only 4% of integration by homologous recombination). PaEXG2 disruption abolished all detectable extracellular exo-beta-1,3-glucanase activity in vitro and in situ but did not affect biocontrol of Botrytis cinerea on wounded apples.  相似文献   

Recovery of Salmonella enterica from seropositive finishing pig herds     

Lo Fo Wong DM  Dahl J  van der Wolf PJ  Wingstrand A  Leontides L  von Altrock A 《Veterinary microbiology》2003,97(3-4):201-214

The aim of this study was to assess the probability of detecting Salmonella from pen faecal samples in seropositive classified finishing pig herds. The study involved 77 herds from Denmark (20), The Netherlands (20), Greece (17) and Germany (20). The serological herd status was determined by the blood-sampling of 50 finishing pigs. Bacteriological sampling was performed by 20 pen faecal samples per herd. Over-all, 47% of the blood samples had an OD% larger than 10 and 23% larger than 40. Salmonella was isolated from 135 (9.3%) pen faecal samples in 32 herds (42%). Twenty-eight of these herds (87.5%) had a within-herd seroprevalence larger than 50% at sample cut-off OD% > 10. In our study, there was an increasing probability of recovering Salmonella with increasing within-herd seroprevalence. However, this was only a moderate correlation. A correlation coefficient of 0.62 was found between the proportion of culture positive- and seropositive samples in a herd at cut-off OD%> 10 and of 0.58 at cut-off OD% > 40. Serology is a measure of historical exposure, which may or may not correlate closely to the microbiological burden at the time of sampling. Due to the low sensitivity of culture methods, apparent 'false-positive' serological results may well represent real infections not detected by bacteriological testing. For screening purposes, serological testing provides an indication of exposure to Salmonella, which forms the basis for targeted sampling, intervention and logistic slaughter procedures.  相似文献   

Resonant formation of DNA strand breaks by low-energy (3 to 20 eV) electrons     

Boudaïffa B  Cloutier P  Hunting D  Huels MA  Sanche L 《Science (New York, N.Y.)》2000,287(5458):1658-1660

Most of the energy deposited in cells by ionizing radiation is channeled into the production of abundant free secondary electrons with ballistic energies between 1 and 20 electron volts. Here it is shown that reactions of such electrons, even at energies well below ionization thresholds, induce substantial yields of single- and double-strand breaks in DNA, which are caused by rapid decays of transient molecular resonances localized on the DNA's basic components. This finding presents a fundamental challenge to the traditional notion that genotoxic damage by secondary electrons can only occur at energies above the onset of ionization, or upon solvation when they become a slowly reacting chemical species.  相似文献   

Relationship between levels of beta-carotene, vitamin A and vitamin E in the seminal plasma, spermatozoa, blood serum and liver of rainbow trout     

Naziroglu M  Gür S  Simsek H  Köprücü K 《The Veterinary record》2000,147(17):484-486

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Cold vapor atomic absorption determination of mercury in coal     

B W Bailey  F C Lo 《Journal of the Association of Official Analytical Chemists》1971,54(6):1447-1448

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Toxic elements in the sex organs of the cow and their relationship to fertility     

E Kudlác  I Simoník 《DTW. Deutsche tier?rztliche Wochenschrift》1990,97(10):396-398

The content of heavy metals (Cd, Hg, Pb, Cr, Cu and Zn) has been determined by atomic absorption spectrophotometry in parts of genital organs of cows with and without reproductive disorders, in blood, in placenta, in colostrum, and in the calf blood. The samples were collected in an industrially polluted area and in a relatively non-polluted area. It has been proven in the group of 99 cows that the biological quality of the oestrous cervical mucus is also influenced by toxic elements. The best conception rate after the artificial insemination (80.0%) has been found in cows with the cervical mucus without spermiotoxic properties. The genital organs of 49 cows culled because of reproductive disorders were examined. No significant differences in the content of heavy metals have been found in the separate parts of genital organs. There were also no differences between the cows with and without reproductive disorders. Toxic elements in blood have been found in cows after parturition and in newborn calves both in the polluted and the non-polluted areas. However, significant differences (p less than 0.01) were determined only in the content of Cd and Zn. The average content of all determined toxic elements in the samples of placenta in pluriparous and primiparous cows from the polluted and non-polluted areas was approximately at the same level. The content of Zn was 4.3 times higher than the hygienic standard in the colostrum of cows from the polluted area. The content of Zn exceeded the hygienic standard also in the non-polluted area, and the content of Cd and Hg was close to the hygienic standard there.  相似文献