Dietary ascorbic acid requirement for growth of striped catfish,Pangasianodon hypophthalmus (Sauvage, 1878) juveniles          下载免费PDF全文

N. Daniel  A.P. Muralidhar  P.P. Srivastava  K.K. Jain  K. Pani Prasad  J. Manish  T. Sivaramakrishnan 《Aquaculture Nutrition》2018,24(1):616-624

A sixty‐day feeding trial was conducted to determine the ascorbic acid (AA) requirement for growth of striped catfish, Pangasianodon hypophthalmus juveniles. Seven iso‐nitrogenous and iso‐energetic (370 g protein per kg and 19.6 MJ/kg) purified diets were prepared with different levels of ascorbic acid such as control (0), T₁ (17.5), T₂ (35), T₃ (70), T₄ (175), T₅ (350) and T₆ (700) mg ascorbic acid (L‐ascorbyl‐2‐polyphosphate) equivalent per kg diet. Fish with a mean body weight of 3.2–3.4 g were stocked (fifteen fish per tank) in triplicates following a completely randomized design. Each group was fed to satiation twice a day for 60 days. Significant differences were observed in growth, survival, body composition and metabolic enzymes activities with different dietary ascorbic acid levels. Maximum weight gain, specific growth rate (SGR) and protein efficiency ratio (PER) were found in fishes fed with 35 mg AA per kg diet, supported by best feed conversion. Fish fed a diet containing vitamin C had the highest activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) compared to those fed with vitamin C‐depleted diets. In this study, based on using broken‐line regression analysis, the dietary vitamin C requirement for growth of P. hypophthalmus juveniles was estimated to be in the range of 46–76 mg AA per kg, depending on the criterion used, growth and liver storage. Our results will be helpful for the formulation of cost‐effective ascorbic acid incorporated diets for striped catfish, P. hypophthalmus.  相似文献   

Differential expression of ISG 15 mRNA in peripheral blood mononuclear cells of nulliparous and multiparous pregnant versus non‐pregnant Bos indicus cattle          下载免费PDF全文

NP Soumya  DN Das  S Jeyakumar  S Mondal  A Mor  UT Mundhe 《Reproduction in domestic animals》2017,52(1):97-106

Embryonic mortality is found to be the main source of reproductive wastage in domestic ruminants. Many genes are involved in the growth and development of the embryo, and the interferon‐stimulated gene 15 (ISG 15) is one of the major gene stimulated by interferon tau, the maternal recognition of pregnancy signal in ruminants. In this study, both genomic and cDNA sequences of ISG 15 from Bos indicus (Deoni breed) were amplified and characterized. The genomic sequence of Deoni ISG 15 exhibited 99% identity with Bos taurus and 97% identity with that of Bos mutus and Bubalus bubalis. Moreover qRT‐PCR analysis revealed constitutive expression of the ISG 15 mRNA in peripheral blood mononuclear cells of Deoni heifers and multiparous cows during early pregnancy. Fourteen Deoni heifers and fifteen multiparous Deoni cows were synchronized for timed AI by CIDR‐Ovsynch protocol, and six animals were kept as cyclic control in each group. Blood samples were collected on days 7, 14, 16, 18, 21, 30 and 45 from the day of AI. Pregnancy was confirmed by plasma progesterone level through ELISA. A significantly higher expression of ISG 15 mRNA was found on day 16 (p < .05) and day 18 (p < .05) of pregnancy in nulliparous heifers. Although in multiparous Deoni cows ISG 15 expression was greater in pregnant cows, difference was statistically non‐significant. The result of this study indicates that ISG 15 gene expression is upregulated during 16–18 days of pregnancy and could be used as an early pregnancy marker in dairy cows especially in heifers.  相似文献   

Diversity and structural composition of species in dipterocarp forests: a study from Fasiakhali Wildlife Sanctuary,Bangladesh     

S. C. Das  M. S. Alam  M. A. Hossain 《林业研究》2018,29(5):1241-1249

Fasiakhali Wildlife Sanctuary is a protected area composed of tropical remnant rainforest that harbor substantial number of large,old Garjan(Dipterocarpus spp.)trees.The present study assessed composition,structure and diversity of the species in this protected area.A total of 32 trees species were recorded with DBH ≥ 11 cm belonging to 24 genera and 19 families.The forest is low in plant diversity as represented by Shannon–Wiener diversity and Simpson Dominance indices.Dipterocarpus turbinatus was the most dominant species with maximum relative density,frequency,dominance,and importance value index.Syzygium firmum and Tectona grandis followed in terms of dominance.The structural composition indicated higher number of individuals in the medium growth classes(41 to 511 cm DBH and 16–20 m height ranges),whereas D.turbinatus was the only species that dominated most of the growth classes.Poor stem density in lower growth classes indicated meager recruitment of regeneration which may be due to lower annual precipitation,increased grazing and encroachments.This study will help to understand the patterns of tree species composition and diversity in the remnant dipterocarp forests of Bangladesh.It will also contribute to identifying threatened plants to undertake D.turbinatus based conservation and sustainable management of the Fasiakhali Wildlife Sanctuary.  相似文献   

Assessment of tree species diversity and its distribution pattern in Pachamalai Reserve Forest,Tamil Nadu     

Suganthi Kanagaraj  Muthu Selvaraj  Rajiv Das Kangabam 《Journal of Sustainable Forestry》2017,36(1):32-46

An increased understanding of the importance of forest diversity for ecosystem services such as medicine, wood, climate control, etc., has lead to greater interest in the study of forests. This study aims to analyze the diversity, composition, and distribution pattern of tree species in Pachamalai Reserve Forest in the Eastern Ghats of Tamil Nadu State. Data were collected in the quadrant method, where 10 × 10 m² plots at different altitudes from 230 to 930 m with 50-m regular intervals were plotted. A total of 73 tree species belonging to 35 families with the density of 1,372 individuals were enumerated from 3 ha. Overall, the most dominant tree species family was Fabaceae (21 species). The highest number in terms of density of tree species was observed for Pongamia pinnata and the most dominant on the basis of basal area was Tamarindus indica. The species inventory was calculated by Shannon-Weiner index (3.916), Simpson index (0.0206), and Margalef index (9.9667). With the increasing altitudinal gradient, the maximum species richness was observed in the middle elevation zone from 580 to 780 m. The results from the study could be helpful for monitoring and sustaining the forest resources, and important in preventing the loss of biodiversity and mitigating climate change.  相似文献   

A Non‐Reciprocal Autosomal Translocation 64,XX,t(4;10)(q21;p15) in an Arabian Mare with Repeated Early Embryonic Loss          下载免费PDF全文

S Ghosh  PJ Das  F Avila  BK Thwaits  BP Chowdhary  T Raudsepp 《Reproduction in domestic animals》2016,51(1):171-174

Balanced autosomal translocations are a known cause for repeated early embryonic loss (REEL) in horses. In most cases, carriers of such translocations are phenotypically normal, but the chromosomal aberration negatively affects gametogenesis giving rise to both genetically balanced and unbalanced gametes. The latter, if involved in fertilization, result in REEL, whereas gametes with the balanced form of translocation will pass the defect into next generation. Therefore, in order to reduce the incidence of REEL, identification of translocation carriers is critical. Here, we report about a phenotypically normal 3‐year‐old Arabian mare that had repeated resorption of conceptuses prior to day 45 of gestation and was diagnosed with REEL. Conventional and molecular cytogenetic analyses revealed that the mare had normal chromosome number 64,XX but carried a non‐mosaic and non‐reciprocal autosomal translocation t(4;10)(q21;p15). This is a novel translocation described in horses with REEL and the first such report in Arabians. Previous cases of REEL due to autosomal translocations have exclusively involved Thoroughbreds. The findings underscore the importance of routine cytogenetic screening of breeding animals.  相似文献   

Swine coronaviruses (SCoVs) and their emerging threats to swine population,inter-species transmission,exploring the susceptibility of pigs for SARS-CoV-2 and zoonotic concerns     

Jigarji C. Thakor  Murali Dinesh  Rajendran Manikandan  Suresh Bindu  Monalisa Sahoo  Diptimayee Sahoo  Manish Dhawan  Megha Katare Pandey  Ruchi Tiwari  Talha Bin Emran  Kuldeep Dhama  Wanpen Chaicumpa 《The Veterinary quarterly》2022,42(1):125

Swine coronaviruses (SCoVs) are one of the most devastating pathogens affecting the livelihoods of farmers and swine industry across the world. These include transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine respiratory coronavirus (PRCV), porcine hemagglutinating encephalomyelitis virus (PHEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), and porcine delta coronavirus (PDCoV). Coronaviruses infect a wide variety of animal species and humans because these are having single stranded-RNA that accounts for high mutation rates and thus could break the species barrier. The gastrointestinal, cardiovascular, and nervous systems are the primary organ systems affected by SCoVs. Infection is very common in piglets compared to adult swine causing high mortality in the former. Bat is implicated to be the origin of all CoVs affecting animals and humans. Since pig is the only domestic animal in which CoVs cause a wide range of diseases; new coronaviruses with high zoonotic potential could likely emerge in the future as observed in the past. The recently emerged severe acute respiratory syndrome coronavirus virus-2 (SARS-CoV-2), causing COVID-19 pandemic in humans, has been implicated to have animal origin, also reported from few animal species, though its zoonotic concerns are still under investigation. This review discusses SCoVs and their epidemiology, virology, evolution, pathology, wildlife reservoirs, interspecies transmission, spill-over events and highlighting their emerging threats to swine population. The role of pigs amid ongoing SARS-CoV-2 pandemic will also be discussed. A thorough investigation should be conducted to rule out zoonotic potential of SCoVs and to design appropriate strategies for their prevention and control.  相似文献   

Indicators of soil quality and crop productivity assessment at a long-term experiment site in the lower Indo-Gangetic plains     

Partha Sarathi Ghorai  Sunanda Biswas  Tapan Jyoti Purakayastha  Nayan Ahmed  Tapas Kumar Das  Radha Prasanna  Bharat Hanamant Gawade  Kallol Bhattacharyya  Kanchan Sinha  Priya Singh  Sujit Das 《Soil Use and Management》2023,39(1):503-520

Deterioration of soil quality under resource-intensive modern agriculture in the face of global climate change poses a huge risk to food security. Because of the complex nature, estimators of soil quality often rely upon a limited set of soil attributes, along with statistical data reduction techniques, for developing quality indices, whilst overlooking biological aspects and regional climatic variability. This study screened the most suitable soil quality indexing approaches for a rice-oilseed-based cropping system in the lower Indo-Gangetic plains (IGP). For this, surface soil samples (0–15 cm) were collected from an ongoing long-term fertilizer experiment with a rice-mustard-sesame cropping system in the IGP. The following treatments were assessed for their effect on soil quality: T₁-control, T₂-NPK (recommended NPK doses), T₃-NPKG (NPK + in situ green manuring), T₄-NPKGB (NPK + in situ green manuring + biofertilizer) and T₅-NPKF (NPK + farm yard manure FYM). We found that total organic carbon (TOC), β-glucosidase, CaCl₂ extractable S, alkaline KMnO₄ oxidizable N, activity of urease, amidase enzyme and mean weight diameter (MWD) were sensitive key indicators of soil quality. The NPKF treatment maintained the highest soil quality status (0.80–0.91), both under productivity and environmental protection goals, owing to the availability of decomposable carbon. Regression analysis showed a better agreement of equivalent rice yield with expert opinion (EO; R² = 0.89) than principal component analysis (PCA; R² = 0.76). Finally, we found that the expert opinion approach with the nonlinear scoring function was the best tool for soil quality assessment of the region.  相似文献