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21.
P.T. Manoharan V. Shanmugaiah N. Balasubramanian S. Gomathinayagam Mahaveer P. Sharma K. Muthuchelian 《European Journal of Soil Biology》2010,46(2):151-156
The present study investigated the effects of arbuscular mycorrhizal (AM) fungus, Glomus mosseae on the growth and physiology state of Erythrina variegata Linn, grown in sandy loam soil with four water stress levels viz. ?0.06 MPa (well watered/control), ?1.20 MPa (mild), ?2.20 MPa (moderate) and ?3.20 MPa (severe) in a completely randomized design. Plants were harvested after 90 days (60 days after stress induction) of growth. Growth parameters (root &, shoot, dry weight and, leaf area); physiological parameters (chlorophyll content, carotenoids, soluble starch, sugar, protein and proline in shoots); and microbiological parameter (percentage of mycorrhizal infection) were determined. AM fungal plants had significantly higher plant biomass, higher chlorophyll content (chlorophyll a and b), carotenoids and protein content in shoots than non-AM-plants. The AM-inoculation in stressed plants significantly declined the soluble sugar and starch in shoots. Moreover, AM-inoculation also reduced the proline accumulation in shoots and the reduction was significant when plants were severely stressed (?3.2 MPa). Mycorrhizal colonization in roots of E. variegata depressed significantly due to increased water stress. However, the AM colonization did not decline below 11% and enabled the plants to maintain osmotic adjustments and enhanced the plants tolerance against water stress. 相似文献
22.
Annamalai Malarvizhi Manoharan Saravanan Rama Krishnan Poopal Jang-Hyun Hur Mathan Ramesh 《Water, air, and soil pollution》2017,228(8):310
Acute (24 h) and sublethal (35 days) effects of cadmium chloride (CdCl2) were examined in Cirrhinus mrigala using various endpoints (accumulation pattern, thyroid hormones (THs), and antioxidants). The mean concentrations of CdCl2 for 24 and 96 h were found to be 35.974 and 22.387 mg L?l, respectively. LC50 concentration of CdCl2 for 24 h (35.97 mg L?l) was used for the acute study. For the sublethal studies, fish were exposed to 3.59 mg L?1 (Treatment I) and 7.19 mg L?1 (Treatment II) corresponding to 1/10th and 1/5th of 24 h LC50 of the CdCl2. During acute exposure, higher accumulation of CdCl2 was noticed in the gill, liver, and kidney of C. mrigala, which is found in the order gill > liver > kidney tissues. Similarly, in sublethal treatments (Treatment I and II), a concentration and time-dependent increase of CdCl2 accumulation was noticed in the order of gill > liver > kidney. GSH, GST, and GPx activities were found to be relatively lower from the treated groups in both acute and sublethal treatments. However, LPO activity was significantly increased in CdCl2-treated fish C. mrigala. Further, plasma T3 reduction was more pronounced than T4 in acute study. During sublethal treatments, both T4 and T3 levels showed a continuous decrease as the exposure period extended. All the values in this study were statically significant (P < 0.01 and P < 0.05). 相似文献
23.
Manoharan S Parthiban M Prabhakar TG Ravikumar G Koteeswaran A Chandran ND Rajavelu G 《Veterinary research communications》2004,28(4):339-346
An immunocomb-based dot-ELISA, employing specially designed apparatus, was used to measure the antibody status for the three major poultry diseases--Newcastle disease, infectious bursal disease and infectious bronchitis--in single test sera. Positive samples could be classified into strong, moderate and weak positives by comparison with the colour reaction given by known strong and weak positive serum controls. The simultaneous dot-immunobinding assay gave reproducible results and allowed considerable savings on the cost of reagents compared to liquid ELISA. The antigen-coated immunocomb can be stored under refrigeration and the test can be performed rapidly under field conditions by trained personnel. 相似文献
24.
R. Balasubramanian K. Balakrishnan S. Manoharan 《Journal of Agronomy and Crop Science》1992,168(1):10-12
Experiment conducted with nine rice varieties during Pishanum season of 1989–90 revealed that Co 37 performed better in ratoon crop and recorded 3.2 t/ha grain yield. It was evident that the ratooning ability was influenced by stem thickness of stubble, carbohydrate content and leaf senescence of main crop. The study also suggests that by maintaining higher seed size and productive tiller in ratoon crop, there is ample possibilities to increase grain yield. 相似文献
25.
Sindumani A. Manoharan Rebecka S. Hess 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(2):726
BackgroundIncreased risk of neoplasia in humans with diabetes mellitus (DM) is well documented. It is unknown if dogs with DM have increased risk of neoplasia.ObjectiveDetermine if dogs with DM have an overall increased risk of neoplasia and risk for specific forms of neoplasia compared to dogs without DM.AnimalsSeven hundred dogs with DM and 700 breed, age, and sex‐matched dogs without DM, examined during the same years.MethodsRetrospective case‐control study. Odds ratios (OR), corresponding 95% confidence intervals (CI), and P‐values were calculated using conditional logistic regression to determine if dogs with DM had increased odds of developing neoplasia compared to dogs without DM.ResultsThe overall odds of developing neoplasia were not significantly different in dogs with and without DM. However, dogs with DM had significantly higher odds of developing an adrenal mass (OR, 4; 95% CI, 1.1‐14.2; P = .03) compared to dogs without DM. The odds of developing a splenic mass in dogs with DM (OR, 1.2; 95% CI, 0.99‐1.39) were increased compared to dogs without DM, but this difference was not significant (P = .07).Conclusions and Clinical ImportanceDogs with DM may be at increased risk for adrenal neoplasia. Awareness of this risk can facilitate early diagnosis of this life‐threatening comorbidity. Larger studies are needed to confirm these findings. 相似文献
26.
Kowsalya R Vishwanathan P Manoharan S 《Pakistan journal of biological sciences: PJBS》2011,14(11):619-626
Chemoprevention, a useful and attractive approach in experimental oncology, helps to investigate the cancer preventive potential of natural products and synthetic entities. Present study evaluated the chemopreventive potential of glycyrrhetinic acid in 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumor was developed in the buccal pouch of golden Syrian hamsters by painting with DMBA three times a week for 14 weeks. The tumor incidence and the status of phase I and phase II detoxification enzymes were assessed in hamsters treated with DMBA alone and DMBA+glycyrrhetinic acid treated hamsters. One hundred percent tumor formations, which were histopathologically confirmed as well-differentiated squamous cell carcinoma, were observed in hamsters treated with DMBA alone. Also, the status of detoxification enzymes were markedly altered in the liver and buccal mucosa of hamsters treated with DMBA alone. Oral administration ofglycyrrhetinic acid at a dose of 45 mg kg(-1) body weight to hamsters treated with DMBA completely prevented the tumor formation as well as restored the status of detoxification enzymes. Present study thus demonstrated the chemopreventive potential of glycyrrhetinic acid in DMBA induced oral carcinogenesis. 相似文献
27.
Cinnamaldehyde, its derivatives and curcumin are reported to have strong antifungal activity. In this work we report and compare anticandidal activity of curcumin (CUR) and α-methyl cinnamaldehyde (MCD) against 38 strains of Candida (3; standard, fluconazole sensitive, 24; clinical, fluconazole sensitive, 11; clinical, fluconazole resistant). The minimum inhibitory concentrations (MIC90) of CUR ranged from 250 to 650 μg/ml for sensitive strains and from 250 to 500 μg/ml for resistant strains. MIC90 of MCD varied between 100 and 250 μg/ml and 100–200 μg/ml for sensitive and resistant strains, respectively. Higher activity of MCD as compared to CUR was further reinforced by spot assays and growth curve studies. At their respective MIC90 values, in the presence of glucose, average inhibition of H+-efflux caused by CUR and MCD against standard, clinical and resistant isolates was 24%, 31%, 32% and 54%, 52%, 54%, respectively. Inhibition of H+-extrusion leads to intracellular acidification and cell death, average pHi for control, CUR and MCD exposed cells was 6.68, 6.39 and 6.20, respectively. Scanning electron micrographs of treated cells show more extensive damage in case of MCD. Haemolytic activity of CUR and MCD at their highest MIC was 11.45% and 13.00%, respectively as against 20% shown by fluconazole at typical MIC of 30 μg/ml. In conclusion, this study shows significant anticandidal activity of CUR and MCD against both azole-resistant and sensitive clinical isolates, MCD is found to be more effective. 相似文献
28.
Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N') of Riemerella anatipestifer. 总被引：9，自引：0，他引：9
Bin Huang Sumathi Subramaniam Joachim Frey Hilda Loh Hai-Meng Tan Charlene J Fernandez Jimmy Kwang Kim-Lee Chua 《Veterinary microbiology》2002,84(3):219-230
The generation of protective immunity against Riemerella anatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (GST) fusion's proteins of OmpA, a 42kDa major outer membrane protein, and P45N', a 41kDa N-terminal fragment of a newly identified 45kDa potential surface protein from R. anatipestifer. The DNA encoding OmpA and P45N' were isolated from R. anatipestifer serotype 15 (field strain 110/89) and serotype 19 (reference strain 30/90), respectively. Immunoblotting and ELISA results showed that the purified recombinant proteins induced the production of antibodies in immunized ducks. However, neither was protective against subsequent challenge with the virulent serotype 15 strain, 34/90. All the five ducks immunized with formalinized R. anatipestifer strain 34/90 survived the challenge with the homologous strain whereas six out of seven ducks in the non-immunized control group died within a week following the challenge. 相似文献
29.
Papadakis SJ De Poortere EP Manoharan HC Shayegan M Winkler R 《Science (New York, N.Y.)》1999,283(5410):2056-2058
Experiments on a constant-density two-dimensional hole system in a gallium arsenide quantum well revealed that the metallic behavior observed in the zero-magnetic-field temperature dependence of the resistivity depends on the symmetry of the confinement potential and the resulting spin splitting of the valence band. 相似文献