Toxicity of insecticides to the sweetpotato whitefly (Hemiptera: Aleyrodidae) and its natural enemies   总被引：1，自引：0，他引：1  

Bacci L  Crespo AL  Galvan TL  Pereira EJ  Picanço MC  Silva GA  Chediak M 《Pest management science》2007,63(7):699-706

Efficient chemical control is achieved when insecticides are active against insect pests and safe to natural enemies. In this study, the toxicity of 17 insecticides to the sweetpotato whitefly, Bemisia tabaci (Gennadius), and the selectivity of seven insecticides to natural enemies of this insect pest were evaluated. To determine the insecticide toxicity, B. tabaci adults were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion, methamidophos, bifenthrin, cypermethrin, deltamethrin, esfenvalerate, fenitrothion, fenpropathrin, fenthion, phenthoate, permethrin and trichlorphon at 50 and 100% of the field rate (FR), and to water (untreated control). To determine the insecticide selectivity, adults of Encarsia sp., Acanthinus sp., Discodon sp. and Lasiochilus sp. were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion and methamidophos at 50 and 100% FR, and to water. Groups of each insect species were exposed to kale leaves preimmersed in each treatment under laboratory conditions. Mortality of exposed individuals was recorded 24 h after treatment. Cartap and imidacloprid at 50 and 100% FR and abamectin and acetamiprid at 100% FR showed insecticidal activity to B. tabaci adults. Abamectin at 50 and 100% FR was the least insecticidal compound to the natural enemies Acanthinus sp., Discodon sp. and Lasiochilus sp. The present results suggest that abamectin at 100% FR may decrease B. tabaci field populations but can still be harmless to predators. Implications of these results within an integrated pest management context are discussed.  相似文献   

Detection of a novel hemoplasma based on 16S rRNA gene DNA in captive and free-ranging capybaras (Hydrochaeris hydrochaeris)     

Rafael F.C. Vieira  Marcelo B. Molento  Leonilda C. dos Santos  Wanderlei Moraes  Zalmir S. Cubas  Andrea P. Santos  Ana M.S. Guimaraes  Ahmed Mohamed  Ivan R. Barros Filho  Alexander W. Biondo  Joanne B. Messick 《Veterinary microbiology》2009,139(3-4):410-413

Two different species of hemoplasmas, Mycoplasma coccoides and M. haemomuris, are known to infect small rodents such as mice and rats. However, there are no previous reports of hemoplasma infection in capybara (Hydrochaeris hydrochaeris). The aim of our study was to determine whether these hemoplasmas might infect capybaras from Southern Brazil. Blood samples from 31 animals: 10 captive and 21 free-ranging capybaras were collected and packed cell volume and total plasma protein were measured. DNA was extracted and PCR assays for M. coccoides and M. haemomuris were performed. Using the M. coccoides-PCR assay 64% of the capybaras were positive, 80% free-ranging and 30% from captive animals. The prevalence of infection between the groups was significantly different (p = 0.001). Sequencing of the nearly entire 16S rRNA gene from the positive samples suggested a novel hemoplasma isolate with identity of 92% with M. coccoides and 86% with M. haemomuris. All capybara samples were negative for M. haemomuris infection. DNA of a housekeeping gene was successfully amplified from all samples. This is the first evidence of a hemoplasma infection in capybaras.  相似文献   

Characterisation of biofilm formation by a Streptococcus suis meningitis isolate     

Grenier D  Grignon L  Gottschalk M 《Veterinary journal (London, England : 1997)》2009,179(2):292-295

Biofilm formation by a strain of Streptococcus suis serotype 2 isolated from a case of meningitis in pigs was characterised. Using a polystyrene microtitre plate assay, S. suis 95-8242 produced a dense biofilm when glucose, fructose or sucrose was used as the carbohydrate source, whereas no biofilm formed in the presence of lactose. Polysaccharide production by the biofilm-forming strain was demonstrated by the Congo red agar assay. Transmission electron microscopy revealed that bacterial cells were surrounded by a thick layer of polycationic ferritin-labelled material. S. suis 95-8242 was more resistant to both penicillin G and ampicillin in biofilms than in planktonic cultures on the basis of minimal inhibitory and minimal bactericidal concentrations.  相似文献   

Serological and molecular evidence of hepatitis E virus in swine in Brazil     

dos Santos DR  Vitral CL  de Paula VS  Marchevsky RS  Lopes JF  Gaspar AM  Saddi TM  Júnior NC  Guimarães Fde R  Júnior JG  Ximenes LL  Souto FJ  Pinto MA 《Veterinary journal (London, England : 1997)》2009,182(3):474-480

Active hepatitis E virus (HEV) infections in two Brazilian swine herds were investigated. In study 1, 26 piglets born to five anti-HEV positive sows were monitored from birth to post-partum week 22. Serum samples were screened for the detection of anti-HEV antibodies and a nested RT-PCR used to examine the HEV genome. Passive transfer of immunity was confirmed. At week 22, 23/26 (88.4%) of the piglets had seroconverted. Genome amplification was achieved in a feces pool from one holding pen and in one serum sample, both from 13-week-old animals. Histology was suggestive of a potential HEV infection. In the second study, 47 piglets born to six anti-HEV-positive sows were monitored after weaning. Seroconversion was determined in eight animals at 6-8 weeks of age. HEV RNA was detected in two pools from a holding pen for 12-16-week-old animals. Brazilian isolates were classified as genotype 3. This is the first molecular evidence of HEV infection in Brazilian pig herds.  相似文献   

Seed blends of pyramided Cry/Vip maize reduce Helicoverpa zea populations from refuge ears     

Guo  Jianguo  Oyediran  Isaac  Rice  Marlin E.  Brown  Sebe  Dimase  Marcelo  Lin  Shucong  Walker  Wade  Yu  Wenbo  Niu  Ying  Huang  Fangneng 《Journal of pest science》2021,94(3):959-968

Journal of Pest Science - The Vip3A, a vegetative Bacillus thuringiensis (Bt) gene, has been introduced into many Cry maize and cotton varieties that can manage the recently occurred...  相似文献   

Effect of Nitrogen and Trinexapac-Ethyl Rates on the SPAD Index of Wheat Leaves     

Marcelo Curitiba Espindula  Valterley Soares Rocha  Paulo Cezar Rezende Fontes  Raul Carvalho Corrêa da Silva  Leandro Torres de Souza 《Journal of plant nutrition》2013,36(11):1956-1964

ABSTRACT

The objective of this study was to evaluate the effect of nitrogen (N) and trinexapac-ethyl (TE) rates on the SPAD index in wheat flag leaf. The treatments were five N rates (30, 60, 90, 120, 150 kg ha^?1) combined with four TE rates (0, 63, 125, 188 g ha^?1). The experiment was carried out in a randomized block design with four repetitions. SPAD index, leaf N content and grain yield showed quadratic response to the increase in N rates, whereas area, wet and dry weight of flag leaf presented linear increase. TE caused linear increase in SPAD index, linear decrease in leaf area, reduction in grain yield with smaller N rates and increase with larger N rates. The N content, and the wet and dry weight of flag leaf were not affected by TE. SPAD calibration to estimate N status in wheat should be specific for each TE rate.  相似文献   

Genetic diversity and path analysis for nitrogen use efficiency in popcorn inbred lines     

Gabriel Borges Mundim  José Marcelo Soriano Viana  Ciro Maia  Geísa Pinheiro Paes  Rodrigo Oliveira DeLima 《Euphytica》2013,191(2):291-299

Popcorn inbred lines with more efficient nitrogen use are better able to uptake nitrogen from the soil and convert it into higher grain yield, resulting in lower environmental and economic impacts caused by nitrogen fertilization. The objectives of this study were to (i) identify inbred lines superior in nitrogen use (ii) assess the genetic diversity between popcorn inbred lines under high and low N conditions and (iii) investigate the causal effects of several traits in nitrogen use efficiency (NUE). We evaluated 25 popcorn inbred lines under high and low N, and several traits related to NUE and its components were measured. Efficient and inefficient inbred lines under both N levels were identified and can be useful for generating a segregating population for quantitative trait loci mapping. The genetic diversity assessment based on phenotypic traits grouped the inbred lines into four clusters under both N levels, and the efficient inbred lines were grouped together, as were the inefficient lines. The most divergent inbred lines under high N were classified as efficient and intermediate and can be useful to generate a divergent breeding population with a high frequency of favorable genes for NUE. Nitrogen uptake efficiency (NUpE) was considered the most important trait for NUE. To improve the accuracy of selection for NUE, a selection index involving the total root length (TRL), daily growth and NUpE traits under both N levels is recommended, as these traits had high correlations with and direct effects on NUE.  相似文献   

Flaxseed (Linum usitatissimum L.) and Its Total Non-digestible Fraction Influence the Expression of Genes Involved in Azoxymethane-induced Colon Cancer in Rats     

Marcelo Hernández-Salazar  Ramón G. Guevara-González  Andrés Cruz-Hernández  Lorenzo Guevara-Olvera  Luis Arturo Bello-Pérez  Eduardo Castaño-Tostado  Guadalupe Loarca-Piña 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(3):259-267

The influence of flaxseed (Linum usitatissimum L.) and its total non-digestible fraction (TNDF) on the expression of genes involved in azoxymethane (AOM)-induced colon cancer in Sprague Dawley rats was analyzed. The dose used in the animal model was two tablespoons of flaxseed per day, which is the dose recommended for humans. Flaxseed significantly decreased the crypt multiplicity (10.50?±?3.5) compared with the AOM treatment (34.00?±?11.0), which suggests that flaxseed exhibits a preventive effect against colon cancer. Both treatments (flaxseed and TNDF) influence the overexpression of genes involved in cell cycle arrest and mitochondrial apoptosis: p53, p21, bcl-2, bax and caspase-3. Flaxseed induced the expression of p53 and p21, whereas TNDF triggered the p21-independent expression of p53. This finding suggests that both of these treatments induced cell cycle arrest. In addition, TNDF induced mitochondrial apoptosis because the TNDF + AOM group exhibited the expression of caspase-3, decreased bcl-2 expression and increased bax expression. These results suggest that the expression of the analyzed genes is associated with the presence of dietary antioxidants linked to the cell wall of flaxseed.  相似文献   

Relationship Between Height Growth and Foliar Nutrient N and P Concentration in a Eucalyptus grandis Plantation in Northeastern Argentina     

Sarah L. Burns  Carolina A. Pérez  Marcelo F. Arturi  Juan F. Goya  Corina Graciano 《Journal of Sustainable Forestry》2013,32(4):313-320

The relationship between the height growth, foliar concentrations of nitrogen (N) and phosphorus (P), and the N:P ratio was analyzed in a fourth rotation plantation that did not respond to fertilization with these nutrients. Spearman's nonparametric correlation analysis was used to evaluate the association between growth in height—calculated as the difference between heights measured at 1 yr and 2 yr after planting—and foliar N and P concentrations, as well as N:P ratio, determined in the 1-yr-old plantation. Only a weak positive correlation between height growth and foliar P concentration in planted seedlings was found. A significant negative relation was observed between the growth and N:P ratio in seedlings. Foliar concentrations suggest that there were neither P nor N limitations for growth in the analyzed period. This may be due to the management of harvest residues, which were abandoned on-site without burning.  相似文献   

Identification of origin and analysis of population structure of field-selected imidazolinone-herbicide resistant red rice (<Emphasis Type="Italic">Oryza sativa</Emphasis>)     

Ives Clayton Gomes dos Reis Goulart  Marcelo Teixeira Pacheco  Anderson Luis Nunes  Aldo MerottoJr 《Euphytica》2012,187(3):437-447

Several red rice biotypes have evolved resistance to imidazolinone herbicides. The origin of resistance has been attributed to gene flow from the herbicide-resistant rice cultivars, but independent evolution of spontaneous mutation can also contribute to the herbicide resistance. The objective of this study was to quantify the occurrence of gene flow and independent selection as the mechanisms of origin of imidazolinone-resistance in red rice to correctly define the management practices for red rice control. Three single nucleotide polymorphism markers were used to identify acetolactate synthase (ALS) gene mutations, and four simple sequence repeat markers were used to identify hybrids of imidazolinone-resistant rice cultivars and red rice. In addition, genetic diversity and population structure analyses were performed. Artificial hybrids were used as controls. Gene flow was the main origin of imidazolinone herbicide resistance, but independent selection occurred in 1.1 % of the evaluated red rice plants. Two red rice plants that independently evolved herbicide resistance had the ALS gene mutation, Gly₆₅₄Glu. Population structure analysis also indicated intense gene flow from rice cultivars to red rice, but some populations maintained a high genetic identity based on a small amount of gene introgression from the rice cultivars. These results indicate the importance of adopting controls of red rice escapees to avoid gene flow from the imidazolinone-resistant rice, and the necessity of biotechnological approaches to mitigating gene flow in the development of new herbicide-resistant rice cultivars.  相似文献