Bone morphogenetic protein 15 supplementation enhances cumulus expansion,nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes     

Juliana de Carvalho Delgado  Thais Rose dos Santos Hamilton  Camilla Mota Mendes  Adriano Felipe Perez Siqueira  Marcelo Demarchi Goissis  José Buratini  Mayra Elena Ortiz D’Ávila Assumpção 《Reproduction in domestic animals》2021,56(5):754-763

In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus-oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production.  相似文献   

Novel Borrelia genotypes in bats from the Macaregua Cave,Colombia     

Sebastin Muoz‐Leal  lvaro A. Faccini‐Martínez  Jairo Prez‐Torres  Sandra M. Chala‐Quintero  Maria T. Herrera‐Sepúlveda  Claudia Cuervo  Marcelo B. Labruna 《Zoonoses and public health》2021,68(1):12-18

Bats have been implicated as reservoirs of relapsing fever group spirochaetes since the beginning of the last century. Recently, bat‐associated spirochaetes have been reported as human pathogens. In 1968, a spirochaete was detected in blood of the bat Natalus tumidirostris captured inside the Macaregua cave, Colombia. Data on this microorganism were never published again. The aim of this study was to evaluate the presence of Borrelia DNA in blood from bats of Macaregua cave. We performed molecular analyses using a genus‐specific real‐time PCR targeting the 16S rRNA to detect DNA of Borrelia in blood samples from 46 bats captured in the Macaregua cave. Positive samples were submitted to a battery of PCRs aiming to amply Borrelia 16S rRNA, flaB, glpQ, p66, ospC, clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA genes. Seventeen samples were positive for Borrelia after real‐time PCR. With the exception of flaB gene, attempts to amplify further loci were unsuccessful. Nucleotide and amino acid divergences of four flaB haplotypes characterized from blood of Carollia perspicillata showed Borrelia burgdorferi sensu lato (Bbsl) as the most closely related group. A phylogenetic tree including 74 sequences of the genus confirmed this trend, since Borrelia genotypes detected in bats from Macaregua formed a monophyletic group basally positioned to Bbsl. Our results suggest that Borrelia genotypes characterized from bats roosting in the Macaregua cave might constitute a new taxon within the genus. This is the first molecular characterization of a Borrelia sp. in Colombia.  相似文献   

Maize grain production,plant nutrient concentration and soil chemical properties in response to different residue levels from two previous crops     

Juan Hirzel  Pablo Undurraga  Lorenzo León  Marcelo Panichini  Jorge Carrasco  Jorge González 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(4):285-293

ABSTRACT

The incorporation of previous crop residues in agricultural management benefits soil fertility, crop production, and environment. However, there is no enough information about maximum residue application level without negative effect over next crop yield. To evaluate maize (Zea mays L.) yield under short-time conservation management with incorporation and/or importation of different residue levels, a biannual rotation experiment was conducted in ash volcanic soil in south-central Chile. The experiment consisted of two previous crops, canola (Brassica napus L.) and bean (Phaseolus vulgaris L.), and four levels of residue incorporation (0%, 50%, 100%, and 200% of generated residue; from 0 to 21.4?Mg?ha^?1 for canola and from 0 to 19.0?Mg?ha^?1 for bean). Previous crop species and residue level affected some nutrients concentrations in grain and plant and some soil chemical properties, without effect in maize yield, which averaged 16.6?Mg?ha^?1. Bean residue increased Ca and reduced S in maize plant, increasing soil P, Ca, Mg and K (P?<?0.05). Maize grain Ca content was positively and proportionally affected by canola residue level and negatively and proportionally affected by bean residue level. All canola residue levels increased soil pH and Mg, but the highest level reduced soil S; soil P concentration increased proportionally with bean residue level. The highest bean residue level increased soil S. Different crop and levels of residue did not affect maize yield but did some plant nutrient concentration, and also affected some soil chemical properties.  相似文献   

Environmental variables influencing regeneration of Nothofagus pumilio in a system with combined aggregated and dispersed retention     

Guillermo J. Martínez Pastur  Juan M. CelliniMaría V. Lencinas  Marcelo BarreraPablo L. Peri 《Forest Ecology and Management》2011,261(1):178-186

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The world of undergraduate education. Brazil. 'I do not make a distinction between teaching and research'     

Leite M 《Science (New York, N.Y.)》2007,317(5834):70-71

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Activation of central melanocortin pathways by fenfluramine     

Heisler LK  Cowley MA  Tecott LH  Fan W  Low MJ  Smart JL  Rubinstein M  Tatro JB  Marcus JN  Holstege H  Lee CE  Cone RD  Elmquist JK 《Science (New York, N.Y.)》2002,297(5581):609-611

D-fenfluramine (d-FEN) was once widely prescribed and was among the most effective weight loss drugs, but was withdrawn from clinical use because of reports of cardiac complications in a subset of patients. Discerning the neurobiology underlying the anorexic action of d-FEN may facilitate the development of new drugs to prevent and treat obesity. Through a combination of functional neuroanatomy, feeding, and electrophysiology studies in rodents, we show that d-FEN-induced anorexia requires activation of central nervous system melanocortin pathways. These results provide a mechanistic explanation of d-FEN's anorexic actions and indicate that drugs targeting these downstream melanocortin pathways may prove to be effective and more selective anti-obesity treatments.  相似文献   

Brominated compounds from marine sponges of the genus Aplysina and a compilation of their 13C NMR spectral data     

Lira NS  Montes RC  Tavares JF  da Silva MS  da Cunha EV  de Athayde-Filho PF  Rodrigues LC  da Silva Dias C  Barbosa-Filho JM 《Marine drugs》2011,9(11):2316-2368

Aplysina is the best representative genus of the family Aplysinidae. Halogenated substances are its main class of metabolites. These substances contribute greatly to the chemotaxonomy and characterization of the sponges belonging to this genus. Due to their pharmacological activities, these alkaloids are of special interest. The chemistry of halogenated substances and of the alkaloids has long been extensively studied in terrestrial organisms, while the number of marine organisms studied has just started to increase in the last decades. This review describes 101 halogenated substances from 14 species of Aplysina from different parts of the world. These substances can be divided into the following classes: bromotyramines (A), cavernicolins (B), hydroverongiaquinols (C), bromotyrosineketals (D), bromotyrosine lactone derivatives (E), oxazolidones (F), spiroisoxazolines (G), verongiabenzenoids (H), verongiaquinols (I), and dibromocyclohexadienes (J). A compilation of their (13)C NMR data is also part of the review. For this purpose 138 references were consulted.  相似文献   

Organic and Conventional <Emphasis Type="Italic">Coffea arabica</Emphasis> L.: A Comparative Study of the Chemical Composition and Physiological,Biochemical and Toxicological Effects in Wistar Rats     

Carvalho Ddo C  Brigagão MR  dos Santos MH  de Paula FB  Giusti-Paiva A  Azevedo L 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):114-121

Differentiation between organic and conventional coffee has increased due to the growing demand and high consumption of healthy foods that contain compounds with antioxidant potential, which have been associated with the reduction of chronic diseases. We used organic and conventional coffee in powder 4% (w/w) and infusions 5%, 10% and 20% (w/v) incorporated in a commercial diet to test in vivo. The levels of chlorogenic acid, caffeine and trigonelline were determined by high performance liquid chromatography. The body weight, weight gain, food consumption, aberrant foci crypt, mucin depleted foci, stress biomarkers protein carbonyl and malondialdehyde, biochemical parameters and behavior of the rats were compared between the experimental and control groups within a framework of colon carcinogenesis. The organic coffee showed higher levels of chlorogenic acid, caffeine and trigonelline than conventional, however, this difference did not significantly affect behavior. The infusions had an antioxidant effect, reducing the levels of malondialdehyde; however, the biochemical parameters of the serum were not altered, and there was neither induction nor prevention of preneoplasic lesions.  相似文献   

Conservation and use of genetic resources of cacao (Theobroma cacao L.) by gene banks and nurseries in six Latin American countries     

Ceccarelli  Viviana  Lastra  Sphyros  Loor Sol&#;rzano  Rey Gast&#;n  Chac&#;n  Walter Wenceslao  Nolasco  Mario  Sotomayor Cantos  Ignacio Antonio  Plaza Avell&#;n  Luis Fernando  L&#;pez  Diana Aracelly  Fern&#;ndez Anchundia  Fabian Marcelo  Dessauw  Dominique  Orozco-Aguilar  Luis  Thomas  Evert 《Genetic Resources and Crop Evolution》2022,69(3):1283-1302

Genetic Resources and Crop Evolution - Cacao (Theobroma cacao L.) is among the most important cash crops in tropical countries. The existing cacao genetic diversity represents a key resource to...  相似文献   

Natural occurrence of Beauveria bassiana on adults of the invasive mango seed weevil Sternochetus mangiferae (Coleoptera: Curculionidae) in Brazil     

Silva  Alessandra Carvalho  Ricalde  Marcelo Perrone  Scalzer  Raíza Ritielle Carvalho  Zilli  Jerri Edison  Lopes  Rogerio Biaggioni 《植物病害和植物保护杂志》2022,129(1):79-84

Journal of Plant Diseases and Protection - The mango seed weevil Sternochetus mangiferae (Fabricius) (Coleoptera: Curculionidae) was detected in Brazil in 2014, becoming a potential threat to mango...  相似文献