Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma     

Samuel White  Meriel Moore‐Colyer  Eliane Marti  Laurent Coüetil  Duncan Hannant  Eric A. Richard  Marcos Alcocer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(5):2327-2335

  相似文献   

Intestinal microbiota of broilers submitted to feeding restriction and its relationship to hepatic metabolism and fat mass: Fast‐growing strain     

Raquel Lunedo  Luiz R. Furlan  Miguel F. Fernandez‐Alarcon  Gustavo H. Squassoni  Daniel M. B. Campos  Dani Perondi  Marcos Macari 《Journal of animal physiology and animal nutrition》2019,103(4):1070-1080

The present study was conducted to verify how feed restriction affects gut microbiota and gene hepatic expression in broiler chickens and how these variables are related to body weight gain. For the experiment, 21‐d‐old Cobb500^TM birds were distributed in a completely randomized experimental design with three treatments: T1. Control (ad libitum—3.176 Mcal/kg ME—metabolizable energy—and 19% CP—crude protein); T2. Energetic restriction (2.224 Mcal/kg ME and 19% CP) from 22 to 42 days with consumption equivalent to control; T3. Quantitative restriction (70% restriction, i.e., restricted broilers ingested only 30% of the quantity consumed by the control group—3.176 Mcal/kg ME and 19% CP) for 7 days, followed by refeeding ad libitum from 28 to 42 days. Ileum and caecum microbiota collections were made at 21, 28 and 42 days of age. Hepatic tissue was collected at 28 and 42 days old for relative gene expression analyses. At 43‐d‐old, body composition was quantified by DXA (Dual‐energy X‐ray Absorptiometry). Both feed restriction programmes decreased Lactobacillus and increased Enterococcus and Enterobacteriaceae counts. No differences were found in the refeeding period. Energetic restriction induced the expression of CPT1‐A (Carnitine palmitoyltransferase 1A) gene, and decreased body fat mass. Quantitative feed restriction increased lipogenic and decreased lipolytic gene expression. In the refeeding period, CPT1‐A gene expression was induced, without changing the broilers body composition. Positive associations were found between BWG (Body Weight Gain) and Lactobacillus and Clostridium cluster IV groups, and negatively associations with Enterobacteriaceae and Enterococcus bacterial groups. In conclusion, differences found in microbiota were similar between the two feed restriction programmes, however, hepatic gene expression differences were only found in quantitative restriction. Higher counts of Lactobacillus and Clostridium cluster IV groups in ileum are likely to be related to better broiler performance and low expression of lipogenic genes.  相似文献   

Genetic association between different criteria to define sexual precocious heifers with growth,carcass, reproductive and feed efficiency indicator traits in Nellore cattle using genomic information     

Martin Bonamy  Sabrina Kluska  Elisa Peripolli  Marcos Vinícius Antunes de Lemos  Sabrina Thaise Amorim  Roberto Jose Vaca  Raysildo Barbosa Lbo  Letícia Mendes de Castro  Carina Ubirajara de Faria  Fabio Borba Ferrari  Fernando Baldi 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2019,136(1):15-22

The aim of this study was to estimate genetic parameters for different precocious calving criteria and their relationship with reproductive, growth, carcass and feed efficiency in Nellore cattle using the single‐step genomic BLUP. The reproductive traits used were probability of precocious calving (PPC) at 24 (PPC24), 26 (PPC26), 28 (PPC28) and 30 (PPC30) months of age, stayability (STAY) and scrotal circumference at 455 days of age (SC455). Growth traits such as weights at 240 (W240) and 455 (W455) days of age and adult weight (AW) were used. Rib eye area (REA), subcutaneous fat thickness (SFT), rump fat thickness (RFT) and residual feed intake (RFI) were included in the analyses. The estimation of genetic parameters was performed using a bi‐trait threshold model including genomic information in a single‐step approach. Heritability for PPC traits was moderate to high (0.29–0.56) with highest estimates for PPC24 (0.56) and PPC26 (0.50). Genetic correlation estimates between PPC and STAY weakened as a function of calving age. Correlation with SC455, growth and carcass traits were low (0.25–0.31; ?0.22 to 0.04; ?0.09 to 0.18, respectively), the same occurs with RFI (?0.09 to 0.08), this suggests independence between female sexual precocity and feed efficiency traits. The results of this study encourage the use of PPC traits in Nellore cattle because the selection for such trait would not have a negative impact on reproductive, growth, carcass and feed efficiency indicator traits. Stayability for sexual precocious heifers (PPC24 and PPC26) must be redefined to avoid incorrectly phenotype assignment.  相似文献   

Influence of storage time and processing on chemical composition and in vitro ruminal fermentation of olive cake     

Carlos N. Marcos  Trinidad de Evan  Paloma García‐Rebollar  Carlos de Blas  Maria Dolores Carro 《Journal of animal physiology and animal nutrition》2019,103(5):1303-1312

Olive oil extraction generates olive cake (OC) that could be used in ruminant feeding. However, the chemical composition of OC is affected by multiple factors, being therefore highly variable. The objective of this study was to analyse the influence of storage time and further processing: crude, exhausted (subjected to a second oil extraction) and cyclone (obtained from a cyclone separator) on nutritive value of OC samples. Twelve samples (six crude and six exhausted) were obtained monthly from the same pond from 1 to 6 storage months, and nine samples (three crude, three exhausted and three cyclone) were obtained monthly from a different pond from 6 to 9 months storage. Chemical composition was analysed, and OC samples were fermented in vitro with sheep rumen fluid. Increasing storage time up to 6 months decreased sugars and total soluble polyphenols content but increased fibre content in OC. Dry matter effective degradability (DMED) decreased linearly (p < 0.001) by 35.9 and 45.5% as storage time augmented from 1 to 6 months for crude and exhausted OC, respectively. Crude OC had lower DMED values than exhausted OC (averaged values 0.255 and 0.294 g/g, respectively). Both potential production and rate of gas production were lower (p ≤ 0.018) in crude compared with exhausted OC, which was attributed to the high fat content of crude OC (≥86 g/kg dry matter). For samples stored longer than 6 months, cyclone had greater (p < 0.05) DMED than crude and exhausted OC (averaged values 0.207, 0.164 and 0.164 g/g, respectively). The results indicate that ruminal degradability of OC is reduced with advancing storage time, but only subtle changes were observed during the first two months. Cyclone showed greater degradability than crude and exhausted OC, but differences between crude and exhausted OC became negligible after five storage months.  相似文献   

Comparison of demographic data,disease severity and response to treatment,between dogs with atopic dermatitis and atopic‐like dermatitis: a retrospective study     

Larissa S. Botoni  Sheila M. F. Torres  Sandra N. Koch  Marcos B. Heinemann  Adriane P. Costa‐Val 《Veterinary dermatology》2019,30(1):10-e4

  相似文献   

Unilateral polydactyly in two foals     

A. Gray  S. Marcos  A. Thomas  O. M. Lepage 《Equine Veterinary Education》2019,31(9):462-467

This case report describes unilateral supernumerary digits of the forelimb in two foals. A diagnosis of polydactyly of the right forelimb in one case and of the left forelimb in the other was made by clinical examination and radiographic interpretation. Surgical excision was performed under general anaesthesia with a good cosmetic and functional outcome in both cases.  相似文献   

Nutritional evaluation and productivity of supplemented sheep grazing in semiarid rangeland of northeastern Brazil     

Araújo  A. R.  Rodriguez  N. M.  Rogério  Marcos Cláudio Pinheiro  Borges  I.  Saliba  E. O. S.  Santos  S. A.  Pompeu  R. C. F. F.  Fernandes  F. E. P.  Monteiro  J. P.  Muir  J. P. 《Tropical animal health and production》2019,51(4):957-966

Tropical Animal Health and Production - Sheep production systems in Brazilian caatinga rangelands require supplementation adapted to changes in floristic and chemical composition as dry seasons...  相似文献   

Relationship between performance,metabolic profile,and feed efficiency of lactating beef cows     

Souza  Luana Lelis  Zorzetto  Mariana Furtado  Ricci  Túlio José Terra  Canesin  Roberta Carrilho  Dias e Silva  Nhayandra Christina  Negrão  João Alberto  dos Santos Gonçalves Cyrillo  Joslaine Noely  Mercadante  Maria Eugênia Zerlotti 《Tropical animal health and production》2019,51(7):2045-2055

Tropical Animal Health and Production - Twenty-seven Nellore cow-calf pairs were submitted for feed efficiency testing. The animals were weighed every 21 ± 5&nbsp;days to...  相似文献   

Protective activity against oocyst shedding in cats vaccinated with crude rhoptry proteins of the Toxoplasma gondii by the intranasal route     

Garcia JL  Navarro IT  Biazzono L  Freire RL  da Silva Guimarães Junior J  Cryssafidis AL  Bugni FM  da Cunha IA  Hamada FN  Dias RC 《Veterinary parasitology》2007,145(3-4):197-206

This study evaluated a vaccine made from crude rhoptry proteins of Toxoplasma gondii with Quil-A, which was administered to cats by the intranasal route. Eleven short-hair domestic cats were divided into four groups: G1 (n=3) received three doses (200 microg/dose) of the rhoptry vaccine with Quil-A (20 microg); G2 (n=3) received PBS with Quil-A (20 microg); G3 (n=3) and G4 (n=2) received only PBS. Treatments were administered at days 0, 21, and 42 by the intranasal route. Challenge was done to G1, G2, and G3 animals with 600 cysts of the VEG strain on day 51 (challenge day); G4 animals were unchallenged. The anti-T. gondii IgG and IgA antibody levels from sera were measured by indirect enzyme-linked immunosorbent assay (ELISA). At challenge, two animals from G1 revealed antibody levels for both IgG and IgA; oocysts were not detected in feces of these two cats. There were no differences in hematological values between groups throughout the experiment (p>0.10). Preventable fractions were 67% in G1 and 0% in G2 and G3. Comparatively, G1 animals shed 89.3% and 90.8% less oocysts than G3 and G4, respectively. Two out of three cats were protected against T. gondii oocyst shedding when the rhoptry vaccine was administered by the intranasal route. This is the first study using crude rhoptry proteins as vaccine by the intranasal route in cats to evaluate protection against oocysts shedding.  相似文献   

The recovery of maleic hydrazide from processed potato products     

A. I. Dias  H. J. Duncan 《Potato Research》1999,42(1):95-99

Summary A method used for estimating extractable maleic hydrazide (MH) concentrations in fresh potato material, concentration range 5–17 mg kg⁻¹, was found not to be suitable for processed potato products (10–33% recoveries) although, boiling potato pieces enhanced recovery by 20%. Each step of the determination was examined and a modified procedure developed with particular emphasis on the extraction of MH from the dried potato matrix, and the quality of the HPLC column used. Potato slices and model systems based on filter papers plus additives were used. Recoveries from fried potato slices were 74±6%. Based on the effect of glucose in reducing extractable MH recoveries, it is suggested that the remainder of the MH (20–25%) is converted into a conjugated structure on reaction with dehydrated sugar (Diels-Alder reaction).  相似文献