Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat     

Young-Bae Kwon  Hyun-Woo Kim  Dae-Hyun Roh  Seo-Yeon Yoon  Rong-Min Baek  Jeum-Yong Kim  HaeYong Kweon  Kwang-Gill Lee  Young-Hwan Park  Jang-Hern Lee 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(2):105-109

Recombinant human epidermal growth factor (rhEGF) stimulates the proliferation and migration of epithelial cells in human cell culture systems and animal models of partial-thickness skin wounds. This study investigated the effect of a topical rhEGF ointment on the rate of wound healing and skin re-epithelialization in a rat full thickness wound model, and verified whether or not the rhEGF treatment affected both myofibroblast proliferation and collagen synthesis in the dermis. When rhEGF (10 µg/g ointment) was applied topically twice a day for 14 days, there was significantly enhanced wound closure from the 5th to the 12th day compared with the control (ointment base treatment) group. A histological examination at the postoperative 7th day revealed that the rhEGF treatment increased the number of proliferating nuclear antigen immunoreactive cells in the epidermis layer. In addition, the immunoreactive area of alpha-smooth muscle actin and the expression of prolyl 4-hydroxylase were significantly higher than those of the control group. Overall, a topical treatment of rhEGF ointment promotes wound healing by increasing the rate of epidermal proliferation and accelerating the level of wound contraction related to myofibroblast proliferation and collagen deposition.  相似文献   

Resistance and cross-resistance to insecticides in human head lice from Florida and California   总被引：1，自引：0，他引：1  

Kyong Sup Yoon  Jian-Rong Gao  Si Hyeock Lee  Gerald C. Coles  Terri L. Meinking  David Taplin  John D. Edman  MiwaKo Takano-Lee  J. Marshall Clark   《Pesticide biochemistry and physiology》2004,80(3):192-201

Head lice from Florida (SF-HL) and California (SC-HL) were resistant to permethrin compared with colonized susceptible lice from Panama (PA-HL) (5.5- and 3.4-fold, respectively) and Ecuador (EC-HL) (8.5- and 5.3-fold, respectively). Permethrin-resistant lice were cross-resistant to pyrethrum and DDT. DNA sequencing validated presence of kdr-type mutations (T929I and L932F). Permethrin resistance was synergized by piperonyl butoxide (PBO) in SC-HL. Resistance to malathion in SF-HL (1.4–2.2-fold) and SC-HL (2.1–3.6-fold) was detected. Malathion resistance in SF-HL was synergized by S,S,S-tributylphosphorotrithioate (DEF) and by PBO in SC-HL. Malathion/permethrin-resistant lice from the UK (BR-HL) were synergized by DEF but not synerziged by PBO. PBO protected BR-HL from malathion, indicating suppressed desulfuration. Abamectin resistance in SF-HL (1.7–2.5-fold) and SC-HL (1.8–2.3-fold) was detected. No resistance to lindane was found. Thus, multiple resistance mechanisms against commonly available and widely used pediculicides and insecticides are apparently occurring.  相似文献   

Fatal measles virus infection in Japanese macaques (Macaca fuscata)     

Choi YK  Simon MA  Kim DY  Yoon BI  Kwon SW  Lee KW  Seo IB  Kim DY 《Veterinary pathology》1999,36(6):594-600

An outbreak of natural measles virus infection occurred in a group of Japanese macaques (Macaca fuscata). Over a period of 4 months, 12 of 53 Japanese macaques died following a 2-23-day history of anorexia, diarrhea, and dermatitis. The monkeys were kept in outdoor exhibits but had been moved temporarily into indoor caging and then transferred to new outdoor exhibits. Ten monkeys died while they were in temporary caging, and two monkeys died after they were moved to new outdoor exhibits. The diagnoses were made based on the results of histopathology, immunohistochemistry (IHC), in situ hybridization (ISH), and electron microscopy. Measles virus antigens were detected in the lung, stomach, skin, salivary gland, spleen, and lymph nodes. Tangled, tubular nucleocapsids compatible with paramyxovirus were noted in the lung tissue. As a result of immunosuppression following measles virus infection, various secondary infections including disseminated cytomegalovirus infection, adenoviral and bacterial pneumonia, and Candida albicans-associated gingivitis and esophagitis were noted. The primary infective source or the mode of infection could not be determined in this outbreak, but measles virus may have been transmitted to the monkeys from human visitors while the monkeys were on exhibit.  相似文献   

Effect of challenge dose and route on porcine reproductive and respiratory syndrome virus (PRRSV) infection in young swine   总被引：3，自引：0，他引：3  

Yoon KJ  Zimmerman JJ  Chang CC  Cancel-Tirado S  Harmon KM  McGinley MJ 《Veterinary research》1999,30(6):629-638

Porcine reproductive and respiratory syndrome virus (PRRSV) is perceived to be highly infectious because of the rapid spread of the virus through populations of domestic swine throughout the world. However, no information has been published on the minimum infectious dose of PRRSV and the effect of challenge dose on clinical response. In this experiment, ten groups of pigs (n = 3 per group) were inoculated with one of five different quantities (10(1)-10(5) fluorescent foci units per millilitre) of PRRSV (isolate ISU-P) by either intramuscular or intranasal routes. Clinical signs and body temperature were monitored for 21 days. Serum was collected periodically throughout the study period to monitor the presence of virus in serum and the early immune response of pigs. A 2-mL inoculum containing 10(1) fluorescent foci units of virus per millilitre was found sufficient to achieve infection by either route. Time to onset of clinical signs was highly associated with challenge dose (P < 0.01), regardless of route of exposure. However, no dose- or route-dependent differences in the severity of clinical manifestation were observed. No significant differences in the time of onset or degree of humoural immune response to PRRSV infection were observed between different treatment groups. However, intramuscular exposure appeared to induce a more uniform antibody response compared to intranasal exposure. These results confirmed that PRRSV is highly infectious; a fact that should be taken into consideration when designing strategies for the prevention and control of PRRSV.  相似文献   

Phylogenetic characterization of canine distemper virus isolates from naturally infected dogs and a marten in Korea     

An DJ  Yoon SH  Park JY  No IS  Park BK 《Veterinary microbiology》2008,132(3-4):389-395

We sequenced the hemagglutinin (H) genes from four canine distemper virus (CDV) isolates obtained from three dogs and a marten in Korea. These sequences were included in subsequent H gene-focused phylogenetic tree analysis of 89 CDV strains. This analysis revealed eight clades designated as EU1, EU2, EU3, NA1, NA2, Asia 1, Asia 2 and Vaccine. Three of the Korean isolates (97Jindo, 98Marten and 07D111) occurred in the Asia 2 group that also contains many Japanese CDV strains isolated in 1998. The remaining Korean strain (07Q72) fell into the Asia 1 group. The 21 H protein sequences of 25 Asia 1 strains are generally predicted to bear nine potential N-linked glycosylation sites. In contrast, the 9 H protein sequences of 12 Asia 2 strains had eight potential N-linked glycosylation sites. The remaining strains had six (98Marten and 07D111) and seven (97Jindo) potential N-linked glycosylation sites.  相似文献   

Age-related changes of calbindin D-28k-immunoreactive neurons in the myenteric plexus of gerbil duodenum     

Choi JH  Lee CH  Chung DW  Hwang IK  Won MH  Seong JK  Yoon YS  Lee IS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):343-348

We examined the age-related changes of calbindin D-28k (CB)-immunoreactive neurons and overall populations of neurons in the myenteric plexus of gerbil duodenum using whole mount preparations and immunohistochemistry. The circumference of duodenum increased age-dependently. CB-immunoreactive neurons were observed in all groups, and most of them had the Dogiel type II morphology. The fully developed cobweb-like structures were observed in the myenteric plexus of duodenum at postnatal month (PM) 3 to 24. Although the highest numbers of CB-immunoreactive neurons and overall population were observed in PM 1.5, it is related with significant increase of the size of circumference between PM 1.5 to PM 3. CB-immunoreactive neurons were slightly decreased with age between PM 3 to PM 24. We have also found that whole numbers of myenteric neurons were also significantly decreased in PM 24 group. These results suggest that loss of overall numbers of myenteric neurons and CB-immunoreactive neurons may be related with age-related neurodegeneration and functional loss of duodenum in the gerbil.  相似文献   

Primary subcutaneous leiomyosarcoma of the hamster hind leg     

Yi JY  Kim YH  Yoon BI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):517-520

We report the first case of a primary subcutaneous leiomyosarcoma that originated in the hind leg of a hamster and metastasized to the bone marrow, lung and diaphragm. A 10-month-old female Syrian golden hamster was presented with a large, firm, white subcutaneous mass in the right hind leg. The tumor invaded into the bone marrow and small nodules were also present in the lung and diaphragm; however, no tumor masses were found in the visceral organs. Histologically, the tumors were spindle cell sarcomas, composed of densely packed pleomorphic spindle cells with oval to elongate nuclei and moderate amounts of eosinophilic cytoplasm. Immunohistochemically, the neoplastic cells were positive for vimentin, desmin, and smooth muscle actin, but negative for alpha-sarcomeric actin and S-100. Thus, the diagnosis was primary leiomyosarcoma of the hind leg with metastasis to the bone marrow, lung and diaphragm. To the best of our knowledge, this is the first report of spontaneous primary subcutaneous leiomyosarcoma of the hind leg with distant metastasis in laboratory animals.  相似文献   

Assessment of antibiotic resistance phenotype and integrons in Salmonella enterica serovar Typhimurium isolated from swine     

Rayamajhi N  Kang SG  Kang ML  Lee HS  Park KY  Yoo HS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(10):1133-1137

Salmonella enterica serovar Typhimurium (S. Typhimurium) isolated and identified from swine were subjected for the analysis of antibiotic resistance pattern and clinically important class 1 and 2 integrons. In addition, S. Typhimurium isolates exhibiting ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline and florfenicol (ACSSuTF) resistance pattern as described in most Salmonella enterica serotype Typhimurium definitive type 104 (DT104) were characterized by polymerase chain reaction. All the isolates were resistant to more than four antibiotics and showed the highest resistance to streptomycin (94.1%), followed by tetracycline (90.1%), ampicillin (64.7%), chloramphenicol (56.8%) and gentamicin (54.9%). MIC value for the ten isolates ranged between 0.125-2 mug/ml for ciprofloxacin. Among the beta-lactams used, only one of the isolate exhibited resistance to ceftiofur (MIC 8 microg/ml). Sixty eight percent of these multi drug resistance (MDR) S. Typhimurium isolates carried clinically important class 1 integron with 1kb (aadA) and/or 2kb (dhfrXII-orfF-aadA2) resistance gene cassettes. This study reports the increasing trend of multi drug resistance (MDR) S. Typhimurium with clinically important class 1 integron in pigs. In addition, emergence of the ACSSuTF-type resistance in S. Typhimurium PT other than DT104 may limit the use of resistance gene markers in its detection methods by PCR.  相似文献   

牛生长激素基因第五外显子Msp I多态性在几个牛种中的差异   总被引：4，自引：0，他引：4  

曹红鹤  尹斗鹤  郝金平 《畜牧兽医学报》2000,31(2):191-192

牛生长激素基因位于第 1 9号染色体上 ,含有大约 1 793个核苷酸 ,由 5个外显子及 4个内含子序列组成。为了研究生长激素基因位点在不同牛品种中的多态性差异 ,根据ＧｅｎｅＢａｎｋ的登记号为Ｍ 5 776 4的牛生长激素基因序列 ,设计了一对引物 ,上游引物 :5’ＧＧＧＣＡＧＡＴＣ ＣＴＣＡＡＧＣＡＧＡ 3’ ;下游引物 :5’ＡＴＣＣＴＣＣＣＣＣＴＴＧＣＴＧＴＣＣ 3’。扩增片段从 2 1 6 5位至2 5 0 4位。产物长度为 34 0ｂｐ ,含第五外显子区。以西门塔尔牛 ( 1 2 6头 ) ,鲁西黄牛 ( 1 4头 ) ,纯种皮埃蒙特牛 ( 8头 ) ,皮埃蒙特×南阳杂种牛 …  相似文献   

Synthesis of hydrophilic copolyesters and the characterization of their moisture-related cooling properties     

Chan Sol Kang  Ho Jin Yoon  Choong Hee Hong  Sun Jin Kwon  Hyun Sun Kim  Doo Hyun Baik 《Fibers and Polymers》2015,16(12):2513-2518

A series of copolyesters (Co-PETs) containing poly(ethylene glycol) (PEG), 5-sodiumsulfodimethyl isophthalate (DMS), and dimethyl isophthalate (DMI) were synthesized via the conventional two-step melt-polycondensation method. The synthesized Co-PETs were characterized by ¹H-NMR spectroscopy, FT-IR spectroscopy, differential scanning calorimeter (DSC), and thermogravimetric analyzer (TGA). The DSC results showed that the melting temperature (T _m) and the heats of fusion (ΔH _m) of Co-PETs decreased with increasing the DMS content in Co-PET, while the inclusion of PEG did not affect their thermal properties significantly. The water absorption and the water contact angle of the Co-PET films were found to be significantly affected by the DMS content rather than PEG content. The moisture-related cooling properties of the fabric samples made of Co-PET 5 as well as PET were evaluated by using liquid moisture management tester (MMT) and Q _max measurements. The MMT and Q _max results indicated that Co-PET 5 fabric containing DMS 1.0 mol% and PEG 10.0 wt% in Co-PET seemed to be a good candidate for the fabric having durable cooling effects.  相似文献