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1. The concentrations of 17 beta-oestradiol (E2) and progesterone (P) were determined in the plasma of layer breeder hens at the end of their first laying season, in relation to neoplasms of the oviduct, including magnum tumours and leiomyomas. 2. The plasma concentration of E2 was higher in tumorous than in non-tumorous hens indicating it may be involved in the development of oviduct tumours. 3. The plasma concentrations of P in tumorous and non-tumorous hens were not significantly different, suggesting no active role of P in the aetiology of the tumours. 4. The E2: P ratio was higher in tumorous than in non-tumorous hens. This ratio could be of value in predicting hens at risk of developing oviduct tumours.  相似文献   
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Transboundary animal diseases (TADs) are highly contagious and have the potential to spread very rapidly irrespective of the national borders, causing serious economic losses. These diseases cause high morbidity and mortality in susceptible animal populations. Furthermore, the extent of their consequences may significantly affect the food security and national economics. In Pakistan, the main TADs include rinderpest, peste des petits ruminants, foot and mouth disease and avian influenza. In the present paper the status of these diseases in Pakistan is discussed.  相似文献   
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Imidacloprid has been used as a key insecticide for controlling sucking insect pests of cotton, whereas Spodoptera litura also has been indirectly exposed to this insecticide in Pakistan. To evaluate the risk of resistance evolution and to develop a better resistance management strategy, a field collected population was selected with imidacloprid in the laboratory. Thereafter, fitness cost, realized heritability and cross resistance of imidacloprid resistance in S. litura were investigated. After 14 generations of selection with imidacloprid, S. litura developed a 137.48-fold resistance to the insecticide. Bioassay revealed that this strain showed cross-resistance to acetamiprid (RR 8.52) and a little to lamdacyhalothrin (1.92) but negative cross-resistance was found to methomyl (−0.19). The resistant strain had a relative fitness of 0.38, with substantially lower rates of larval survival, larval duration, male pupal duration, development time, emergence rate of healthy adults, fecundity, hatchability, and prolonged larval and pupal duration. Mean relative growth rate of the larvae, intrinsic rate of population increase, and biotic potential was lower for the selected populations. The estimated realized heritability (h2) of imidacloprid resistance was 0.15 in the resistant strain of S. litura. Development of the resistance may cost significant fitness for the resistant population. This study provided valuable information for further understanding the impact of imidacloprid resistance on physiological parameters of S. litura and for facilitating the development of resistance management strategies.  相似文献   
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Estimating variation in grain mineral concentration and bioavailability in relation to grain yield and the year of cultivar release is important for breeding wheat with increased content of bioavailable minerals. The grain yield and yield components, grain phytate concentration, and concentration and bioavailability of minerals (zinc Zn, iron Fe and calcium Ca) in wheat grains were estimated in 40 wheat cultivars released in Punjab (Pakistan) during the last five decades. Mean grain Zn and Ca concentrations in current-cultivars were significantly lower (≥14%) than in obsolete cultivars released during the Green Revolution (1965–1976). Much of this variation was related to increased grain weight in current-cultivars. There was a positive correlation among minerals (r = 0.39 or higher, n = 40) and minerals with phytate in wheat grains (r = 0.38 or higher, n = 40). The tested cultivars varied widely in grain yield and grain phytate-to-mineral molar ratios (phytate:mineral). Compared to obsolete cultivars, the current-cultivars had a higher phytate:mineral ratio in grains, indicating poor bioavailability of minerals to humans. The study revealed a non-significant relationship between grain yield and phytate:mineral ratios in grains. Therefore, breeding for lower phytate:mineral ratios in wheat grains can ensure increased mineral bioavailability without significant reduction in the yield potential. Future breeding should be focused on developing new genotypes suitable for mineral biofortification and with increased mineral bioavailability in grains.  相似文献   
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In the present study, embryogenic calli of sugarcane variety BL4 were induced using 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin in different concentrations and combinations. In contrast to earlier studies, embryogenic callus sectors were identified and isolated microscopically within 1–2 weeks. Subsequently, 51 media formulations were used for regeneration of proliferated embryogenic callus, using MS medium supplemented with three different cytokinins [kinetin, 6-Benzylamino purine (BAP), and thidiazuron (TDZ)] and auxins (IAA/NAA and IBA) in different combination and concentrations. After acclimatization, the genomic DNA of regenerated plants was studied to explore the insertion polymorphism of transposable elements in order to ascertain the variation among somaclones. Though low concentration of kinetin with 2,4-D was found supportive to embryogenic callus development, the highest number of regenerated plantlets was observed using BAP (1 μM), however the plantlets had very low fresh weight (2.2 g). Conversely, TDZ alone supported a significant increase in the number of plantlets regenerated (38–40) with higher fresh weight. The somaclones generated during this study showed considerable positional polymorphism of activator-like transposable elements possibly due to the stress associated with in vitro culture. This study provides a procedure to produce regenerated sugarcane plants from embryogenic callus in a relatively short time.  相似文献   
69.
Urea is a common ingredient of the diets of intensively fed lambs, but is increasingly required for industrial processes. Maize steep liquor (MSL) is a by-product of maize grain degradation to produce starch that may be a suitable replacement. Fifty growing lambs were fed on equinitrogenous diets in which between 0% and 80% of the urea was replaced by MSL; their growth and metabolism were recorded over 70 days. Increasing replacement of urea by MSL increased feed intake and nutrient digestibilities, leading to increased growth rates, more efficient feed conversion, and increased nitrogen retention. Concentrations of triiodothyroxin, thyroxin, glucose, and methionine were increased by replacement of urea by liquor, and plasma urea was reduced. This study suggests that MSL is a suitable replacement for up to 80% of urea in the diet of rapidly growing lambs.  相似文献   
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The present paper reports the prevalence of coccidiosis in industrial broiler chickens in Faisalabad, Punjab, Pakistan to determine the occurrence of different species of Eimeria in the area and to assess their correlation with the environmental conditions including temperature, humidity and rainfall. The study was conducted from January 2009 to December 2010. Examination of chicken guts revealed 43.89% (n = 3,283/7,480) prevalence of coccidiosis. The highest prevalence (27.04%) was recorded of Eimeria tenella followed by Eimeria maxima (22.42%), Eimeria acervulina (19.89%) and Eimeria necatrix (4.02%). The prevalence of disease was significantly higher (P < 0.05) in autumn (60.02 ± 4.38) followed by summer (47.42 ± 2.92), spring (36.92 ± 2.27) and winter (29.89 ± 3.43). Likewise, prevalence of different species of Eimeria also varied (P < 0.05) in different seasons of the year except that of E. necatrix. A strong correlation (P < 0.05) of environmental conditions with the overall and species-wise prevalence of coccidiosis was recorded except E. necatrix.  相似文献   
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