Effect of eCG dose on ovarian haemodynamics,hormonal profiles and prolificacy rate when oestrus was induced during low-breeding season in Beetal goats     

Babar Sharif  Mubbashar Hassan  Usman Arshad  Muhammad Z. Tahir  Ejaz Ahmad  Muhammad I. Khan  Muhammad Shahzad  Imran Mohsin  Froylan Sosa  Abdul Rehman 《Reproduction in domestic animals》2023,58(1):48-59

The objectives of the experiment were to determine the effect of two doses of equine chorionic gonadotropin (eCG) in a standard synchronization protocol based on a short-term progesterone (P₄) priming on ovarian structures and haemodynamics, concentrations of steroid hormones and prolificacy rate when oestrus was induced during low-breeding season (LBS) in Beetal dairy goats. We hypothesized that inclusion of eCG in a short-term P₄ priming-based synchronization protocol would increase the blood perfusion to ovarian structures leading to enhance oestrous and ovulatory responses and prolificacy rate in goats. Forty-two multiparous acyclic goats were blocked by body condition and, within block, assigned randomly to receive saline as control (CON), low eCG (L-eCG; 300 IU) or high eCG (H-eCG; 600 IU) dose. Initially, a controlled internal drug release (CIDR) device was placed in the anterior vagina on d −8, followed by removal of CIDR on d −3, concurrent with the administration of PGF_2α and eCG according to their respective treatments. Goats were monitored for oestrous response. B-mode and Doppler ultrasonography was performed with 12-h interval, starting from day −3 until natural breeding (day 0), and then on days 5, 10, 15 and 20 post-breeding to monitor follicular and luteal dynamics and blood flow, respectively. Blood was sampled at 0, 12, 24, 36 and 60 h after CIDR removal to quantify plasma concentrations of estradiol-17β (E₂), whereas plasma concentrations of P₄ were assayed at days 5, 10, 15 and 20 after breeding. Pregnancy and prolificacy rates were determined at day 30 and 150 after breeding, respectively. Data were analysed with mixed-effects models, and orthogonal contrasts were used to evaluate the effect of treatment [Con vs. (½ L-eCG + ½ H-eCG)] and dose of eCG (L-eCG vs. H-eCG). Data are presented in sequence as CON, L-eCG, H-eCG (LSM ± SEM). The oestrous intensity score (152.9 vs. 182.7 vs. 186.5 ± 15.1; p = .02) was greater in eCG-treated goats as compared to CON. Administration of eCG reduced the intervals to standing oestrus (66.2 vs. 41.8 vs. 48.9 h ± 5.5; p = .05), breeding (70.2 vs. 44.4 vs. 45.4 h ± 4.5; p = .03) and ovulation (84.5 vs. 61.2 vs. 63.4 h ± 6.2; p = .05) compared with CON goats. The mean growth rate of pre-ovulatory follicle was greater (1.11 vs. 1.49 vs. 1.45 mm ± 0.08; p = .01) in eCG-treated goats resulting in an increased diameter of pre-ovulatory follicle (6.27 vs. 7.20 vs. 7.31 mm ± 0.07; p < .01) and corpora lutea (6.75 vs. 8.26 vs. 8.07 mm ± 0.42; p = .04) than CON. The mean follicular blood flow did not differ among treatments; however, the mean luteal blood flow was greater in L-eCG-treated goats (0.81 vs. 1.61 vs. 1.07 cm² ± 0.12; p = .001). The mean concentrations of E₂ (4.03 vs. 5.21 vs. 4.78 pg/ml ± 0.42; p = .04) and P₄ (4.85 vs. 6.39 vs. 6.22 ng/ml ± 0.34; p = .04) were greater in eCG-treated goats. The twinning rate did not differ between treatments; nevertheless, prolificacy rate was greater (p = .04) in L-eCG-treated goats. Collectively, our data suggest that the administration of eCG improves the induction of oestrous and ovarian dynamics. Administration of L-eCG enhances prolificacy rate, therefore, a low dose of eCG might be practically beneficial to improve reproduction during LBS in acyclic Beetal dairy goats.  相似文献   

Antioxidant and Anticancer Activities of Walnut (Juglans regia L.) Protein Hydrolysates Using Different Proteases     

Raheleh Jahanbani  S. Mahmood Ghaffari  Maryam Salami  Kourosh Vahdati  Houri Sepehri  Nazanin Namazi Sarvestani  Nader Sheibani  Ali Akbar Moosavi-Movahedi 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(4):402-409

  相似文献   

Using synergistic exogenous phytohormones to enhance somatic embryogenesis from leaf explants of a Eucalyptus grandis clone     

Muhammad Nakhooda  Eshani Mandiri 《Southern Forests》2016,78(1):73-80

Somatic embryogenesis (SE) in Eucalyptus spp. has been limited to germinated seeds, flowers, lignotubers or zygotic embryos. The low yield of somatic embryos from leaf explants has hampered progress, even though leaves offer a more viable source of clonal explants from superior selected genotypes. It was hypothesised that SE from leaf explants could be enhanced through pairing of synergistic exogenous plant growth regulators, such as natural auxins with natural cytokinins. Callus and embryo induction using 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthalene acetic acid (NAA), indole acetic acid (IAA), and indole butyric acid (IBA), each at either 1.0 or 3.0?mg L^?1, indicated that IAA and IBA favoured significantly higher numbers of embryos compared with 2,4-D or NAA. Hence, IAA and IBA were used for subsequent experiments, combining them (at 1.0?mg L^?1) with either the synthetic cytokinin, kinetin, or the natural cytokinin, trans-zeatin, both at 0.1?mg L?1. The combination of trans-zeatin and either IAA or IBA resulted in a significant increase in SE (e.g. 86 ± 17.2% and 23 ± 3.2% for IAA with trans-zeatin and kinetin, respectively), compared with kinetin, or with these auxins alone. Embryo maturation and plantlet regeneration was highest in those calli that were induced with IAA and trans-zeatin, indicating that maturation was dependent on auxin depletion, based on the stability of the analogue used for induction. For the E. grandis clone under study, the use of synergistic plant growth regulators significantly enhanced SE from leaf explants, thus presenting the opportunity to benefit from the advantages that SE offers over conventional organogenesis.  相似文献   

The Effect of Chelating Agents on Enhancement of 1,1,1-Trichloroethane and Trichloroethylene Degradation by Z-nZVI-Catalyzed Percarbonate Process     

Muhammad Danish  Xiaogang Gu  Shuguang Lu  Xiang Zhang  Xiaori Fu  Yunfei Xue  Zhouwei Miao  Ayyaz Ahmad  Muhammad Naqvi  Abdul Sattar Qureshi 《Water, air, and soil pollution》2016,227(9):301

  相似文献   

Boron Application Improves Growth,Yield and Net Economic Return of Rice   总被引：1，自引：1，他引：0  

Mubshar HUSSAIN  M.Ayaz KHAN  M.Bismillah KHAN  Muhammad FAROOQ  Shahid FAROOQ 《水稻科学》2012,19(3):259-262

A field trial was conducted to evaluate the role of boron (B) application at different growth stages in improving the growth,yield and net economic return of rice at farmer’s fields during summer season,2009.Boron was soil applied (1.5 kg/hm2) at the transplanting,tillering,flowering and grain formation stages of rice;foliar applied (1.5% B solution) at the tillering,flowering and grain formation stages of rice,and dipped seedling roots in 1.5% B solution before transplanting;while control plots did not apply any B.Boron application (except dipping of seedling roots in B solution,which caused toxicity and reduced the number of tillers and straw yield than control) substantially improved the rice growth and yield.However,soil application was better in improving the number of grains per panicle,1000-grain weight,grain yield,harvest index,net economic income and ratio of benefit to cost compared with the rest of treatments.Overall,for improving rice performance and maximizing the net economic returns,B might be applied as soil application at flowering.  相似文献   

Comparative studies on some yield contributing traits of wheat sown in binary mixtures     

Sabina Asghar  Muhammad Kashif  Abdus Salam Khan  Ihsan Khaliq  Jehanzeb Farooq 《Frontiers of Agriculture in China》2011,5(2):141-145

Cultivar mixtures have been suggested as ways to increase crop productivity. This field study was conducted to investigate the competition among five cultivated varieties of wheat (Triticum aestivum L.) for the traits like plant height, flag leaf area, fertile tillers per plant, spike length, spikelets per spike, spike density, grain weight per spike, and seed index in relation to competition with yield per plant. It was observed that cultivar mixtures ought to be more productive than corresponding pure stands. Four binary combinations of Seher2006-Kohistan97, Farid2006-SH2002, SH2002-Shahkar95, and SH2002-Kohistan97 were superior in performance, showing land equivalent ratio (LER) values greater than one for plant height, fertile tillers per plant, spike density, grain weight per spike, and grain yield per plant. It was observed that the performance of binary mixtures was mainly due to the increased average performance in spike density, grain weight per spike, and grain yield per plant. The competitive ability of wheat varieties in mixtures was independent of each other for the respective traits, which lead to cumulative effects for the increased performance within the associates in mixtures.  相似文献   

Screening of mungbean germplasm against mungbean yellow mosaic India virus and its vector Bemisia tabaci     

Khalid P. Akhtar  Ghulam SarwarGhulam Abbas  Muhammad J. AsgharNighat Sarwar  Tariq M. Shah 《Crop Protection》2011,30(9):1202-1209

Yellow mosaic disease (YMD) caused by whitefly (Bemisia tabaci) transmitted mungbean yellow mosaic India virus (MYMIV) is an important constraint of mungbean in Pakistan. One hundred sixty-two mungbean genotypes from eight different geographic regions were evaluated for resistance to MYMIV and its whitefly vector B. tabaci. Resistance levels were assessed by visual scoring of symptoms in the field under natural conditions, in net-house by artificial transmission through whitefly and in greenhouse using graft inoculation for two consecutive years in 2008 and 2009. None of the tested genotypes was found to be disease free, while considerable variation in responses was observed among the genotypes and testing methods. All genotypes were found to be systemically infected in the field. In 2008, 32 genotypes were found to be resistant and 67 were moderately resistant with severity index (SI) values ranging from 1.0 to 1.4 and from 1.5 to 2.4, respectively. In 2009 34 genotypes were found to be resistant and 38 were moderately resistant with the same SI value ranges as in 2008, and the remaining genotypes were moderately susceptible to highly susceptible. However, only 35 genotypes were found to be moderately resistant with SI values of 1.5-2.4 and latent periods (LP) of 17-21 days when evaluated using artificial transmission through whiteflies. Similar results were obtained when these genotypes were evaluated using graft inoculation. Significant differences in adult whitefly densities among tested genotypes were also observed during both years but no correlation between the number of whiteflies and disease severity was observed. The results indicated that these genotypes did not have a high level of resistance against MYMIV, however, they may provide sources of partial resistance which can be exploited in the breeding programmes to develop mungbean genotypes resistant to MYMD or they can be used directly as varieties to manage MYMD after evaluation for acceptable agronomic characteristics, adaptation and stability in various regions.  相似文献   

Optimization of alkaline hydrolysis of paddy straw for ferulic acid extraction   总被引：1，自引：0，他引：1  

Noor Hasyierah Mohd Salleh  Mohamed Zulkali Mohamed Daud Dachyar Arbain  Muhammad Syarhabil AhmadKu Syahidah Ku Ismail 《Industrial Crops and Products》2011,34(3):1635-1640

Response Surface Methodology (RSM) via Central Composite Design (CCD) was used to optimize the alkaline hydrolysis of paddy straw to improve ferulic acid extraction. The parameters involved, namely temperature (°C), concentration of NaOH (M) and duration of extraction (h) are screened by Full Factorial Studies (FFD). The result obtained from FFD was 0.518% (5.18 mg/g). The interactions between each parameters involved were studied using Central Composite Design (CCD). Upon optimization, the percentage of ferulic acid improved from 0.518% to 0.817% (8.17 mg/g). The optimum conditions obtained from this study are 125 °C, 3.90 M, 2.30 h for temperature, concentration of NaOH and extraction time, respectively. This result shows a significant improvement than the previous published work. Details of the experimental design, statistical analysis and interpretation are discussed below.  相似文献   

Alleviation of arsenic toxicity by phosphate is associated with its regulation of detoxification,defense, and transport gene expression in barley     

Gerald Zvobgo  Jonas Lwalaba Wa Lwalaba  Tichaona Sagonda  James Mutemachani Mapodzeke  Noor Muhammad  Imran Haider Shamsi  Guo-ping ZHANG 《农业科学学报》2019,18(2):381-394

Arsenic(As) contamination in soils has posed a severe threat to safe crop production. The previous studies showed the antagonism between phosphorus(P) and As in plant growth and As uptake, while the mechanisms of alleviating As toxicity by P is not completely clear. Due to the limiting P condition, it is imperative to understand how low P addition can be used to suppress arsenate As(V) uptake and the subsequent mechanisms involved. Thus in this study we investigated the effect of P addition on As uptake, anti-oxidative enzyme activity, and anti-oxidant content, and the relative expression of transport, defense, and detoxification genes using two barley genotypes differing in As toxicity tolerance. P addition significantly reduced As concentration in plant tissues, and caused the great changes in activities of catalase and superoxide dismutase, glutathione content, and the relative expression of examined genes when the plants of the two barley genotypes were exposed to 100 μmol L~(–1) As, with ZDB160(As-tolerant) being much more affected than ZDB475(As-sensitive). The current results show that P addition can alleviate As toxicity by regulating the expression of As transport, defense, and detoxification genes to a greater extent in As tolerance of barley, suggesting the possibility of controlling As uptake and toxicity by applying low amount of P fertilizers in the As-contaminated soils.  相似文献   

Sorption and Desorption Behavior of FTuoroquinolone Antibiotics in an Agricultural Soil     

Luqman RIAZ  Tariq MAHMOOD  YANG Qingxiang  Muhammad Wahab YASIR  Audil RASHID  Mark S.COYNE  Elisa D'ANGELO 《土壤圈》2019,(5)

<正>Dear Editor,Fluoroquinolones(e.g.,ciprofloxacin(CIP),enrofloxacin(ENR),and levofloxacin(LEV))are an extensively used family of antibiotics prescribed for various infections;however,they are also increasingly used  相似文献