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61.
The volatile constituents ofTirmania nivea (white desert truffle) have been analysed, using gas chromatography/mass spectrometric technique. 11 compounds have been identified in the ascocarp volatiles. The major components were found to be unsaturated fatty acids; whereas haxadecanoic acid represented 49% of the volatiles isolate.  相似文献   
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A cross-sectional study was conducted to identify risk factors for herd infection by Brucella spp. in dairy cattle in the suburbs of Asmara, Eritrea. Data were collected from 64 herds, randomly selected from a total of 99 herds with a minimum herd size of 9 cows. A questionnaire was used to gather data on management, hygiene and herd structure. Serum samples collected from all pregnant heifers, cows and bulls, were screened for Brucella infection by the Rose Bengal test (RBT), and all RBT-positive sera re-tested with the complement-fixation test (CFT) for confirmation. A seropositive herd was defined as one in which at least one animal tested positive in the CFT. There were 23 (36%) positive herds among the 64 studied. Both multiple logistic and multiple betabinomial regression modeling were used to analyze the data. Mixed-breed herds, compared to single (exotic)-breed herds, were found to be independently associated with increased herd seroprevalence (OR=5.2, 95% confidence interval 1.4–18.7) in the multiple logistic model with the herd infection status as the dependent variable. The importance of this variable was supported by the multiple betabinomial regression model (OR=3.3, 1.4–7.6) with animal-level prevalence within herd as the outcome variable. Both models also revealed the presence of a negative association between seropositivity and cattle stocking density.  相似文献   
64.
The antibacterial activities of the chloroform, methanol and aqueous extracts of Dichrostachys cinerea fruits and leaves are reported.  相似文献   
65.
The antioxidant status of rats experimentally infected with Trypanosoma evansi isolated from a camel was studied using established parasitological, haematological and biochemical methods. The results indicated that infections in all rats resulted in a fulminating parasitaemia. Changes in blood parameters in T. evansi-infected rats indicated leukocytosis and a macrocytic hypochromic anaemia. A degree of anisocytosis was also observed. The activities of plasma glucose-6-phosphate dehydrogenase and glutathione peroxidase in whole blood of infected rats were significantly higher (p<0.05 and p<0.001, respectively) compared with control. No statistically significant difference was observed in the activity of superoxide dismutase in infected and control rats. Results obtained indicated that trypanosomosis caused oxidative stress and induced antioxidant enzymes.  相似文献   
66.
An experiment was conducted to determine the effects of supplemental dietary l-carnitine, chromium picolinate (Cr-Pic) and their combination on growth performance and serum total protein, cholesterol, triglyceride and glucose of rainbow trout (Oncorhynchus mykiss). A total of 276 rainbow trout were randomly allocated to four groups. Fish (average initial body weight = 151 ± 1.69 g) were fed a basal diet without supplemental l-carnitine and chromium picolinate in the control group. The basal diet was supplemented with either 500 mg/kg l-carnitine, 1.6 mg/kg chromium picolinate or 500 mg/kg l-carnitine plus 1.6 mg/kg chromium picolinate for experimental groups 1 (C), 2 (Cr-Pic) and 3 (C + Cr-Pic), respectively. Fish were fed twice a day to apparent satiation for 58 days. Weight gain, growth rate, feed consumption and feed conversion ratio (feed/gain) were calculated for the whole period. At the end of the study, six fish were chosen randomly from each tank to represent the experiment and frozen at −20°C for subsequent dorsal muscle analysis. The results showed no significant differences in final body weight, weight gain or feed conversion ratio among groups. There were also no significant differences in serum total protein, cholesterol, triglyceride or glucose concentrations among groups. Significantly higher lipid concentration of dorsal muscle was observed in experimental groups 1 and 3. In conclusion, supplemental dietary l-carnitine, chromium picolinate and their combination have no beneficial effects on improving growth performance and feed conversion ratio in rainbow trout. However, dietary l-carnitine slightly increased lipid concentration in dorsal muscle of rainbow trout.  相似文献   
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In their natural environment, microbes organize into communities held together by an extracellular matrix composed of polysaccharides and proteins. We developed an in vivo labeling strategy to allow the extracellular matrix of developing biofilms to be visualized with conventional and superresolution light microscopy. Vibrio cholerae biofilms displayed three distinct levels of spatial organization: cells, clusters of cells, and collections of clusters. Multiresolution imaging of living V. cholerae biofilms revealed the complementary architectural roles of the four essential matrix constituents: RbmA provided cell-cell adhesion; Bap1 allowed the developing biofilm to adhere to surfaces; and heterogeneous mixtures of Vibrio polysaccharide, RbmC, and Bap1 formed dynamic, flexible, and ordered envelopes that encased the cell clusters.  相似文献   
69.
Disease incidence and severity was studied for winter wheat variety Bezostaya 1 and susceptible checks based on data from international nurseries from 1969 to 2010 and from 51 countries across major winter wheat production regions totalling 1,047 reports. The frequency of leaf rust and stripe rust occurrence was stable over time with marked increases in severity in 2001–2010 especially in Europe and Central and West Asia. Substantial global reductions in stem rust occurrence were recorded and attributed primarily to use of resistance genes although the recent emergence of race Ug99 makes wheat more vulnerable. The occurrence of powdery mildew remained globally stable over time. It was the most important foliar disease in Western and Southern Europe, where the frequency was very high for all time periods coupled with slight increases in severity during 2001–2010. The durable resistance of variety Bezostaya 1 to all four diseases was demonstrated in the study using comparisons of disease severities of Bezostaya 1 and the most susceptible entries. The Lr34/Yr18/Pm38 pleiotrophic set possessed by Bezostaya 1 is currently an important target for selection because it is now amenable to molecular selection. Increased use of genes like Lr34 combined with strategies to minimize cultivation of extremely susceptible varieties will contribute to long term maintenance of low and non-damaging disease levels. The durable disease resistance of Bezostaya 1, combined with its adaptability and good end-use quality, was a significant reason for its huge impact in agriculture over the last 50?years.  相似文献   
70.
Race-specific resistance against powdery mildews is well documented in small grains but, in other crops such as grapevine, controlled analysis of host-pathogen interactions on resistant plants is uncommon. In the current study, we attempted to confirm powdery mildew resistance phenotypes through vineyard, greenhouse, and in vitro inoculations for test cross-mapping populations for two resistance sources: (i) a complex hybrid breeding line, 'Bloodworth 81-107-11', of at least Vitis rotundifolia, V. vinifera, V. berlandieri, V. rupestris, V. labrusca, and V. aestivalis background; and (ii) Vitis hybrid 'Tamiami' of V. aestivalis and V. vinifera origin. Statistical analysis of vineyard resistance data suggested the segregation of two and three race-specific resistance genes from the two sources, respectively. However, in each population, some resistant progeny were susceptible in greenhouse or in vitro screens, which suggested the presence of Erysiphe necator isolates virulent on progeny segregating for one or more resistance genes. Controlled inoculation of resistant and susceptible progeny with a diverse set of E. necator isolates clearly demonstrated the presence of fungal races differentially interacting with race-specific resistance genes, providing proof of race specificity in the grape powdery mildew pathosystem. Consistent with known race-specific resistance mechanisms, both resistance sources were characterized by programmed cell death of host epidermal cells under appressoria, which arrested or slowed hyphal growth; this response was also accompanied by collapse of conidia, germ tubes, appressoria, and secondary hyphae. The observation of prevalent isolates virulent on progeny with multiple race-specific resistance genes before resistance gene deployment has implications for grape breeding strategies. We suggest that grape breeders should characterize the mechanisms of resistance and pyramid multiple resistance genes with different mechanisms for improved durability.  相似文献   
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