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31.
Field tests at three sites over a period of three to four years have shown that PVYo spread in New Brunswick starts in mid-to late July when plants reach maximum height. Some 62 different genera or species of aphids were collected in experimental plots. Only seven of these were known vector species. Of the colonizing species, the alate green peach aphid,Myzus persicae (Sulzer), seems to be the most important vector. However, because it is not always present when disease spread starts, non-colonizing aphids are probably responsible for the early spread of PVYo. Five of these species of aphids were tested in the laboratory to determine their vector efficiency. A list of potential vectors is presented. The impact of these findings on the management of PVYo is discussed.  相似文献   
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Honey bees (Linnaeus, Hymenoptera: Apidae) are widely used as commercial pollinators and commonly forage in agricultural and urban landscapes containing neonicotinoid-treated plants. Previous research has demonstrated that honey bees display adverse behavioral and cognitive effects after treatment with sublethal doses of neonicotinoids. In laboratory studies, honey bees simultaneously increase their proportional intake of neonicotinoid-treated solutions and decrease their total solution consumption to some concentrations of certain neonicotinoids. These findings suggest that neonicotinoids might elicit a suboptimal response in honey bees, in which they forage preferentially on foods containing pesticides, effectively increasing their exposure, while also decreasing their total food intake; however, behavioral responses in semifield and field conditions are less understood. Here we conducted a feeder experiment with freely flying bees to determine the effects of a sublethal, field-realistic concentration of imidacloprid (IMD) on the foraging and recruitment behaviors of honey bees visiting either a control feeder containing a sucrose solution or a treatment feeder containing the same sucrose solution with IMD. We report that IMD-treated honey bees foraged less frequently (–28%) and persistently (–66%) than control foragers. Recruitment behaviors (dance frequency and dance propensity) also decreased with IMD, but nonsignificantly. Our results suggest that neonicotinoids inhibit honey bee foraging, which could potentially decrease food intake and adversely affect colony health.  相似文献   
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