植物激素乙烯的分子生物学研究进展     

姚瑞亮　关雄李杨瑞  陈如凯 《广西农业生物科学》1999

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Sequence data suggests big liver and spleen disease virus (BLSV) is genetically related to hepatitis E virus.   总被引：6，自引：0，他引：6  

C J Payne  T M Ellis  S L Plant  A R Gregory  G E Wilcox 《Veterinary microbiology》1999,68(1-2):119-125

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An experimental vaccine against Aeromonas hydrophila can induce protection in rainbow trout,Oncorhynchus mykiss (Walbaum)     

S E LaPatra  K P Plant  S Alcorn  V Ostland  J Winton 《Journal of fish diseases》2010,33(2):143-151

A candidate vaccine against Aeromonas hydrophila in rainbow trout, Oncorhynchus mykiss, was developed using a bacterial lysate. To test the strength of protection, A. hydrophila challenge models were compared using injection into both the intraperitoneal (IP) cavity and the dorsal sinus (DS) with selected doses of live bacteria washed in saline or left untreated. Unlike the IP route, injection into the DS with either saline washed or unwashed cells resulted in consistent cumulative mortality and a dose response that could be used to establish a standard challenge having an LD₅₀ of approximately 3 × 10⁷ colony forming units per fish. Survivors of the challenge suffered significantly lower mortality upon re‐challenge than naïve fish, suggesting a high level of acquired resistance was elicited by infection. Passive immunization using serum from hyper‐immunized fish also resulted in significantly reduced mortality indicating protection can be transferred and that some portion of resistance may be antibody mediated. Vaccination of groups of rainbow trout with A. hydrophila lysate resulted in significant protection against a high challenge dose but only when injected along with Freund’s complete adjuvant. At a low challenge dose, mortality in all groups was low, but the bacterial lysate alone appeared to offer some protection.  相似文献   

Vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), with recombinant and DNA vaccines produced to Flavobacterium psychrophilum heat shock proteins 60 and 70     

K P Plant  S E LaPatra  K D Cain 《Journal of fish diseases》2009,32(6):521-534

Flavobacterium psychrophilum heat shock proteins (Hsp) 60 and 70 are highly immunogenic and were therefore investigated as potential vaccine candidates. Recombinant Hsps were purified from Escherichia coli and rainbow trout ( Oncorhynchus mykiss ) were intraperitoneally injected with phosphate buffered saline/Freunds complete adjuvant (FCA), 8 μg of rHsp60/FCA, rHsp70/FCA or a combination of 4 μg each of rHsp60 and rHsp70/FCA. Antibody responses against recombinant Hsp60 and Hsp70 8 weeks post-immunization were observed, but only fish immunized with rHsp70 exhibited highly elevated antibody levels against F. psychrophilum whole cell lysate. Some cross reactivity occurred, which may have been due to the V5 tag common to both proteins. Protection against F. psychrophilum challenge was not observed in any treatments at 8 weeks post-immunization. To further investigate any protective effect of these proteins, hsps were polymerase chain reaction amplified and cloned into pVAX1. Rainbow trout were intramuscularly injected with 8 μg of pVAX1hsp60, pVAX1hsp70 or a combination of 4 μg each of pVAX1hsp60 and pVAX1hsp70. Antibody responses at 4 weeks post-immunization were low and protection was not observed following challenge at 6 or 10 weeks post-immunization. Although Hsps of F. psychrophilum have been shown to be immunodominant, these antigens do not appear to be good vaccine candidates when delivered alone or in combination.  相似文献   

Synthesis,conformational studies and anthelmintic activity of a constrained PF1022A analogue     

Jürgen Scherkenbeck  Hubert Dyker  Daniel Gondol  Achim Harder  Andrew Plant  Felix Reichel 《Pest management science》1999,55(4):457-461

A mono-proline analogue of the potent anthelmintic cyclooctadepsipeptide PF1022A has been synthesized. NMR studies in combination with molecular dynamics simulations demonstrate that the confirmation of this analogue is both rigidified and distorted compared with the asymmetric conformation of PF1022A. The backbone conformation is significant with respect to anthelmintic activity. © 1999 Society of Chemical Industry  相似文献   

水稻白叶枯病菌酸性还原酮加双氧酶的鉴定分析     

夏更寿  张燕  宋从凤  王金生 《植物病理学报》2014,44(1):46-53

 酸性还原酮加双氧酶(ARD)催化很多原核和真核生物中的甲硫氨酸急救途径(MSP)倒数第二步。本研究鉴定了水稻白叶枯病菌Xanthomonas oryzae pv. oryzae (Xoo)中的酸性还原酮加双氧酶, 命名为xard。Xoo 菌株PXO99^A、MAFF311018和KACC10331中的xard核苷酸序列完全相同。xard基因突变菌株在甲硫基腺苷(MTA)为唯一硫源时不能正常生长。这一结果证明Xard在MSP中起作用。xard突变体和野生型菌株PXO99^A 接种水稻IR24后病斑长度数据表明该基因突变对Xoo在水稻上的毒性没有影响。  相似文献   

水稻突变体des2和des5的研究和定位(英文)     

赫圣博  何玮  陈红旗  朱旭东  杨军  胡筱荷  罗达 《广西农业生物科学》2009,(1)

小穗发育是决定水稻产量的主要农艺性状,鉴定控制小穗发育的关键基因对研究和分析调控农艺性状的分子机理是至关重要的。本文中,我们鉴定了一组小穗数目明显减少的突变体,命名为decreased spikelets(des),这里详细研究des2和des5两个突变体。结果显示des2是由单基因隐性位点控制,图位克隆将此位点定位到6号染色体的长臂上,并最终克隆了此基因,发现des2是moc1的一个新的等位突变体。定位克隆和序列分析显示在des5中,LAX基因的编码HLH(螺旋-环-螺旋)结构域的区域发生了一个点突变,暗示des5是lax的一个新的等位突变体。我们的结果暗示小穗和水稻叶腋分枝的发育受相同的遗传途径调控。  相似文献   

cDNA芯片检测棉花纤维发育相关基因的表达     

徐瑜  施永辉  冯建勋  朱玉贤 《分子植物育种》2004,2(3):348-353

利用cDNA芯片技术分析E6、Lipid transfer protein(LTP)、Proline-rich protein(PRP）、Expansin、Tubulin、Annexin等家族的63个棉花纤维发育相关基因,在陆地棉徐州-142开花后10d纤维组织及其无长绒无短绒突变体开花后10d胚珠中的表达差异,发现属于E6、LTP、PRP、Expansin家族的基因在两种组织中存在显著的表达差异,符合前人的报道。其中E6、LTP家族的基因在两种组织中的表达差异最大,个别基因表达差异甚至达到15倍之多。而Tubulin家族的基因在两种组织中的表达差异不大,检测的24个Tubulin家族基因中,仅2个基因在纤维组织和胚珠中存在显著表达差异。cDNA芯片技术可以高通量鉴定棉花纤维相关基因以及研究棉花发育相关基因的表达谱。  相似文献   

抗病虫基因新资源:海洋绿藻孔石莼凝集素基因   总被引：5，自引：0，他引：5  

王盛  钟伏弟  吴祖建  谢联辉  林奇英 《分子植物育种》2004,2(1):153-155

植物凝集素(Lecin)具有抵抗植物病原菌和农业害虫的活性以及抗病毒活性,在植物保护上具有广阔的应用前景。从海洋绿藻孔石莼中分离到一种凝集素基因序列,为培育抗病虫作物新品种提供了新资源。  相似文献   

AFLP analysis of genetic variation of Hyphantria cunea （Drury） populations in Beijing and a nearby site     

TAO Jing  AI Yi-wen    LUO You-qing  YANG Liu  YAN Wei  CHEN MinKey Laboratory for Silviculture  Conservation of Ministry of Education  Beijing Forestry University  Beijing  P.R.China College of Environment  Plant Protection  Hainan University  Danzhou  P.R.China 《中国林学(英文版)》2009,(1)

Hyphantria cunea(Drury) is a severe invasive pest in Beijing.It is important to understand the reason for its successful invasion and outbreak.Accordingly,this study was tailored to assess molecular variation and genetic relationships among three H.cunea populations in Beijing and one population from Sanhe in Hebei Province as control(totally 100 individuals).A silver-labeled amplified fragment length polymorphisms(AFLP) DNA profiling method was used to detect the genetic diversity and relationship among th...  相似文献