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11.
充分发挥本科林业院校的学科、资源优势,紧紧把握高等职业技术教育的特点与需求,重点抓好专业设置,始终坚持产、学、研结合,不断深化教学改革,努力探索一条具有林业特色的高等职业技术教育之路. 相似文献
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法氏囊活性肽对鸡体增重及饲料转化率的影响 总被引:3,自引:0,他引:3
把不同剂量的法氏囊活性肽 (BS)冻干粉与传染性法氏囊病 (IBD)活苗混合后 ,对 2 1日龄SPF鸡滴鼻点眼 ,同时另选一批同样生长状态的鸡 ,在颈部皮下接种IBD细胞毒油苗 ,同时肌肉注射上述不同剂量法氏囊活性肽 ,观察法氏囊活性肽对鸡生长性能的影响。结果发现 :免疫后7d ,活苗 0 2mLBS组增长最快 ,比免疫对照组多增 82 5 % ,并且饲料报酬高。但从整个试验期看 ,0 4mLBS组增重效果较好 ,比免疫对照组多增 1 9 8% ,并且有较高的饲料转化率。而肌肉注射BS组中 ,高剂量 (0 8mL)BS组的增重效果一直很好 ,比对照组总增重平均多增 7 2 3 % ,并有较高的饲料报酬。这说明滴鼻点眼或是肌肉注射 ,BS都有促生长的作用 相似文献
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中国果树类病毒的发生及其研究进展(英文) 总被引:4,自引:0,他引:4
概述了在中国发生且己鉴定明确的5种果树类病毒,即苹果绣果类病毒(Apple scar skin viroid,ASSVd)、梨泡状溃疡类病毒(Pear blister canker viroid,PBCVd)、葡萄黄斑类病毒-1(Grapevine yellow speckle viroid-1,GYSVd-1)、柑橘裂皮类病毒(Citrus exocortis viroid,CEVd)和桃潜隐花叶类病毒(Peachlatent mosaic viroid,PLMVd)的研究进展,包括病害的首次发现、症状特征、发病规律、检测方法与防治对策以及这些类病毒的生物学与分子生物学特性。 相似文献
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邱德玉 《四川畜牧兽医学院学报》2005,3(4):5-8
文化底蕴越是丰厚的旅游资源,开发价值就越高。正是众多文人贤士游剡访古,使浙东剡溪的历史、文化与自然山水完美地结合在一起,形成了绚丽多彩的剡溪山水文化。文化被认为是旅游业的灵魂,是一种潜在的旅游产品。要提高山水旅游产品的文化品位,必须将山水文化的潜在价值转化为旅游产品。本文对剡溪山水文化旅游资源的开发提出了以下设想:再现“唐诗之路”山水旅游美景,并着重于按唐诗及有关文献、传说等增添新景点,沿江、沿山、于水中进行山水、文化全面开发,形成立体式、全景式的剡中山水文化游道;可开发出文化生态、修学、科考、宗教、节庆等剡溪山水文化旅游产品。 相似文献
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AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen. 相似文献
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LING Yun-biao XU Rui-yun QIU Wan-shou CHU Zhong-hua YANG Hong-zhi TAN Zhi OU Qing-jia 《园艺学报》2003,19(12):1649-1652
AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[3H]H]proline and [[3H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P<0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P<0.05). Portal serum decreasea collagen synthesis (P<0.05), but circumferential serum had no effect (P>0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action. 相似文献
18.
AIM: To investigate the effect of berberine (Ber) on the activation and proliferation of T lymphocytes and its mechanism of action. METHODS: Whole peripheral blood from normal subjects was stimulated with phytohemagglutinin (PHA) or phorbol ester (PDB) plus ionomycin (Ion) and the expression levels of CD69 and CD25 were evaluated with flow cytometry after the staining with appropriate fluorescent monoclonal antibody. The distribution of cell cycles was analyzed by propidium iodide staining and dead cells by 7-aminoactinomycin live staining. RESULTS: 100 μmol/L and 50 μmol/L of Ber had significant inhibition of the expression of CD69 on T cells stimulated with PDB plus Ion or PHA, while effect of 25 μmol/L Ber was not significant. And as time of action extended, the extent of inhibition decreased. For the expression of CD25, Ber at the concentrations as above all exerted significant inhibitory effect in a dose-dependent manner. Moreover, Ber could block lymphocytes cell cycle progression from G0/G1 phase to S and G2/M phase without phase specificity. Besides, live staining analysis revealed that Ber did not have significant cytotoxicity on lymphocytes. CONCLUSIONS: Ber significantly inhibits the expression of early and mid activation antigens of T cells and also blocks the progression of lymphocytes cell cycles. These results suggest that Ber exerts immunosuppression effect through inhibiting the activation and proliferation of T cells. 相似文献
19.
昆虫病原线虫rDNA多态性分析 总被引:1,自引:0,他引:1
本文对国内外昆虫病原线虫斯氏属和异小杆属的47个品系进行rDNA—ITS PCR—RFLP分析,研究其DNA多态性,并构建了分子系统发育树状图。各品系的ITS区无明显的长度差异,PCR—RFLP分析将47个品系分为斯氏属和异小杆属两大类,两属线虫又分别分为11组和4组。所得结果丰富了ITS PCR—RFLP图谱库,为弄清我国的昆虫病原线虫与国外种类的分子系统发育关系及未定名线虫的鉴定提供重要依据,同时为筛选适合的线虫种类防治害虫奠定基础。 相似文献
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