Serum vascular endothelial growth factor in dogs with soft tissue sarcomas     

G. Fernandes de Queiroz  M. Lúcia Zaidan Dagli  S. Aparecida Meira  J.  Maria Matera 《Veterinary and comparative oncology》2013,11(3):230-235

This work aimed to evaluate serum vascular endothelial growth factor (VEGF) in 25 dogs with soft tissue sarcoma, and in 30 healthy dogs. Blood was collected once time from the control animals and three times, in the same way, from animals with sarcoma. Blood count was performed in the blood collected, and serum VEGF was measured by enzyme‐linked immunosorbent assay quantitative method. Serum VEGF in control animals was similar to patients with soft tissue sarcoma. There was a reduction in serum VEGF after the sarcoma resection. There was positive correlation between serum VEGF and neutrophil counts, and negative between VEGF and hemoglobin content in animals with sarcoma. Animals with hemangiopericytoma showed higher serum VEGF levels compared to the patients with malignant peripheral nerve sheath. Circulating blood cells can contribute to elevate VEGF serum concentrations in dogs with soft tissue sarcomas and a possible role of VEGF in the angiogenesis of these tumors.  相似文献   

Assessment of the ideal ratios of digestible essential amino acids for pacu,Piaractus mesopotamicus,juveniles by the amino acid deletion method     

Andr Z. Boaratti  Thiago M. T. Nascimento  Kifayat U. Khan  Cleber F. M. Mansano  Thaís S. Oliveira  Daniel M. A. Queiroz  Rafael S. Romaneli  Andressa T. Rodrigues  Nilva K. Sakomura  Joo B. K. Fernandes 《Journal of the World Aquaculture Society》2021,52(1):88-104

The present study aimed to determine the ideal ratios of digestible essential amino acids (EAAs) for pacu (Piaractus mesopotamicus) juveniles by the amino acid (AA) deletion method. A completely randomized design which consisted of 11 treatments and three replicates each was used. The treatments included a control diet (CD) containing 55% of nonpurified natural ingredients and 45% of purified synthetic amino acids and ingredients, and other ten isonitrogenous and isoenergetic EAA limiting diets (LDs), each being deficient in 44.4 ± 0.02% of the respective EAA. Pacu juveniles with initial average body weight of 6.22 ± 0.09 g were distributed among 33 fiber glass tanks. Fish were fed with semipurified and extruded diets for 113 days two times a day until apparent satiation. The ideal ratio of each dietary EAA was calculated on the basis of the relationship between body N retention and amount of EAA deleted from the respective EAA LD. Based on the AA deletion method, the ideal ratios of digestible EAAs for pacu juveniles, relative to lysine requirement of 100% were estimated as: methionine 14.6%, threonine 35.0%, tryptophan 6.6%, arginine 62.8%, histidine 13.6%, isoleucine 26.3%, leucine 43.7%, phenylalanine 27.2%, and valine 35.8%.  相似文献   

A multi-environment trials diallel analysis provides insights on the inheritance of fumonisin contamination resistance in tropical maize     

José Maria Villela Pádua  Kaio Olímpio Das Graças Dias  Maria Marta Pastina  João Cândido de Souza  Valéria Aparecida Vieira Queiroz  Rodrigo Veras da Costa  Maria Beatriz Pereira da Silva  Carlos Alexandre Gomes Ribeiro  Claudia Teixeira Guimarães  Salvador Alejandro Gezan  Lauro José Moreira Guimarães 《Euphytica》2016,211(3):277-285

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Study of Analytical Techniques to Determine Chlorpyrifos in the Surface Waterways of the Rural Zone of Ouro Branco,Brazil: A Case Study     

Taciana Maria da Silveira  Renata Domingos Alves  Maria Eliana Lopes Ribeiro de Queiroz  Antônio Augusto Neves  Maurício Xavier Coutrim  Robson José de Cássia Franco Afonso  Gilmare Antônia da Silva 《Water, air, and soil pollution》2016,227(9):335

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Assessment of Anthropogenic Impacts on the Water Quality of Marreco River,Brazil, Based on Principal Component Analysis and Toxicological Assays     

Soraya Moreno Palácio  Fernando Rodolfo Espinoza-Quiñones  Aline Roberta de Pauli  Pitágoras Augusto Piana  Caroline Bressan Queiroz  Simony Camila Fabris  Márcia Regina Fagundes-Klen  Márcia Teresinha Veit 《Water, air, and soil pollution》2016,227(9):307

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Genetic diversity of the root‐knot nematode Meloidogyne enterolobii and development of a SCAR marker for this guava‐damaging species     

M. Tigano  K. De Siqueira  P. Castagnone‐Sereno  K. Mulet  P. Queiroz  M. Dos Santos  C. Teixeira  M. Almeida  J. Silva  R. Carneiro 《Plant pathology》2010,59(6):1054-1061

The objectives of this work were to evaluate the genetic variability of Meloidogyne enterolobii by molecular markers, and develop species‐specific molecular markers for application in detection. Sixteen M. enterolobii isolates from different geographical regions (Brazil and other countries) and hosts were used in this study. The identification and purification of the populations were carried out based on isoenzyme phenotype. The DNA amplification of the intergenic region (IGS) of the rDNA and of the region between the cytochrome oxidase subunit II (COII) and 16S rRNA genes (mtDNA) produced specific fragments of the expected size for this nematode, i.e. 780 and 705 bp, respectively. Intraspecific variability among the isolates was evaluated with three different neutral molecular markers: AFLP, ISSR and RAPD. The results showed a low level of diversity among the isolates tested, indicating that M. enterolobii is a genetically homogeneous root‐knot nematode species. The RAPD method allowed the identification of a species‐specific RAPD fragment for M. enterolobii. This fragment was cloned and sequenced, and from the sequence obtained, a set of primers was designed and tested. The amplification of a 520‐bp‐long fragment occurred only for the 16 isolates of M. enterolobii and not for the 10 other Meloidogyne species tested. In addition, positive detection was achieved in a single individual female, egg‐mass and second stage juvenile of this nematode. This SCAR species‐specific marker for M. enterolobii represents a new molecular tool to be used in the detection of this nematode from field samples and as a routine diagnostic test for quarantine devices .  相似文献   

Phenolic, polysaccharidic, and lipidic fractions of mushrooms from northeastern Portugal: chemical compounds with antioxidant properties     

Heleno SA  Barros L  Martins A  Queiroz MJ  Santos-Buelga C  Ferreira IC 《Journal of agricultural and food chemistry》2012,60(18):4634-4640

Mushrooms do not constitute a significant portion of the human diet, but their consumption continues to increase due to their functional benefits and presence of bioactive compounds. Some of those compounds can be found in the phenolic, polysaccharidic, and lipidic fractions of edible and inedible species. Herein, those fractions of five wild mushrooms (Coprinopsis atramentaria, Lactarius bertillonii, Lactarius vellereus, Rhodotus palmatus, and Xerocomus chrysenteron) from northeastern Portugal were studied for their chemical composition and antioxidant properties. Protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were found in the phenolic fraction; rhamnose, xylose, fucose, arabinose, fructose, glucose, manose, mannitol, sucrose, maltose, and trehalose were quantified in polysaccharidic fraction; and linoleic and stearic (only in Lactarius sp.) acids, and β- and γ-tocopherols were the main compounds in the lipidic fraction. C. atramentaria and X. chrysenteron phenolic fractions gave the highest free radical scavenging activity, reducing properties, and lipid peroxidation inhibition in brain homogenates, which is in agreement with its highest content in total phenolics. Furthermore, among the polysaccharidic fractions C. atramentaria also gave the highest antioxidant activity, which is in agreement with its highest total polysaccharides content and sugars obtained after hydrolysis.  相似文献   

Blood pressure and BMI in adolescents in Aracaju, Brazil     

Polderman J  Gurgel RQ  Barreto-Filho JA  Roelofs R  Ramos RE  de Munter JS  Wendte JF  Agyemang C 《Public health nutrition》2011,14(6):1064-1070

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Sodium hydroxide or urea pretreatment of acerola (Malpighia emarginata) fruit residue increases dry matter degradability and reduces methane production in in vitro rumen fermentation     

Moura  Lais Micaelle Lopes  da Silva Pereira  Flávia Denise  de Lima  Patrícia Rodrigues  Nascimento  Júlio Cesar Silva  dos Santos de Oliveira  Andresca  de Souza Rodrigues  Rafael Torres  Ferreira  Clébio Pereira  Moraes  Salete Alves  Queiroz  Mário Adriano Ávila  Menezes  Daniel Ribeiro 《Tropical animal health and production》2020,52(5):2433-2441

Tropical Animal Health and Production - The aim of this study was to evaluate the effect of adding different concentrations of either urea or NaOH in dehydrated acerola (Malpighia emarginata) fruit...  相似文献   

Quantitative and descriptive histological aspects of jaguar (Panthera onca Linnaeus, 1758) ear skin as a step towards formation of biobanks     

Érika A. Praxedes  Luiza B. de Queiroz Neta  Alana A. Borges  Maria B. Silva  Maria V. O. Santos  Leandro R. Ribeiro  Herlon V. R. Silva  Alexsandra F. Pereira 《Anatomia, histologia, embryologia》2020,49(1):121-129

Skin of mammals vulnerable to extinction, such as the jaguar, is used as a source of material in conservation strategies. The composition of skin is not uniform among species, and the ability to distinguish similarities in skin morphology in animal groups is fundamental in the application of skin tissue for use in biobanks. The aim of our study was to evaluate the structure, composition and capacity for culture of ear skin from the yellow and black jaguars. Both qualitative and quantitative methods were used, focusing on skin thickness, cell quantification and distribution, collagen density, proliferative activity and viability. Histomorphometrical study of the skin showed a total thickness of 273.2 and 274.6 µm for the yellow and black jaguars, respectively. Melanocytes and fibroblasts were, respectively, 9.7 and 23.0 for the yellow jaguar and 11.3 and 26.8 for the black jaguar. A collagen density of 67.0% and 49.0% was observed for yellow and black jaguars, respectively. Both animals presented a proliferative activity varying between 1.20 and 1.30. All tissues could promote cellular detachment, reaching subconfluence in 10–15 days. This kind of information from histomorphometrical features and cell cultures can be essential for a more targeted application of ear skin cryopreservation in this species, as such information will enable understanding the action of substances on tissues during the conservation process.  相似文献