Does the Use of Chitosan Contribute to Oxalate Kidney Stone Formation?     

Moacir Fernandes Queiroz  Karoline Rachel Teodosio Melo  Diego Araujo Sabry  Guilherme Lanzi Sassaki  Hugo Alexandre Oliveira Rocha 《Marine drugs》2015,13(1):141-158

Chitosan is widely used in the biomedical field due its chemical and pharmacological properties. However, intake of chitosan results in renal tissue accumulation of chitosan and promotes an increase in calcium excretion. On the other hand, the effect of chitosan on the formation of calcium oxalate crystals (CaOx) has not been described. In this work, we evaluated the antioxidant capacity of chitosan and its interference in the formation of CaOx crystals in vitro. Here, the chitosan obtained commercially had its identity confirmed by nuclear magnetic resonance and infrared spectroscopy. In several tests, this chitosan showed low or no antioxidant activity. However, it also showed excellent copper-chelating activity. In vitro, chitosan acted as an inducer mainly of monohydrate CaOx crystal formation, which is more prevalent in patients with urolithiasis. We also observed that chitosan modifies the morphology and size of these crystals, as well as changes the surface charge of the crystals, making them even more positive, which can facilitate the interaction of these crystals with renal cells. Chitosan greatly influences the formation of crystals in vitro, and in vivo analyses should be conducted to assess the risk of using chitosan.  相似文献   

Considerations on the Use of Malachite Green in Aquaculture and Analytical Aspects of Determining the Residues in Fish: A Review     

Juliana Campos Hashimoto  Jonas Augusto Rizzato Paschoal  Júlio Ferraz de Queiroz 《Journal Of Aquatic Food Product Technology》2013,22(3):273-294

Aquaculture in Brazil has shown expressive development since the 1990s with growth rates superior to those of cattle and poultry. In order to achieve greater productivity, intensive fish cultivation systems are employed, which can cause greater susceptibility to diseases caused by viruses, bacteria, fungi, and parasites. The reduced availability of veterinary medications approved for use in aquaculture in Brazil has lead fish farmers to the indiscriminate use of several chemical substances with antimicrobial activity, such as the dye malachite green (MG). As a result of this use, residues of MG and its main biotransformation product, leucomalachite green (LMG), may be present in fish available for consumption. The presence of residues of these compounds represents a risk to human health due to their toxicity, as well as a potential impact on the environment, and could also raise barriers for commercialization in the country and for exportation. The objective of this review is to provide the context and evidence of the use of MG in aquaculture and of its toxicological and legislative aspects. A review of the analytical methods used to determine MG residues in fish, with emphasis on mass spectrometry, is also presented.  相似文献   

A New Application for <Emphasis Type="Italic">Klebsiella oxytoca</Emphasis> in Bioremediation: Treatment of Manganese-Laden Wastewaters     

Natália R. Barboza  Mônica M. Cordeiro  Pricila A. Santos  Pollyana S. Queiroz  Versiane A. Leão  Renata Guerra-Sá 《Water, air, and soil pollution》2018,229(1):19

Bioremediation is a method of removing manganese from wastewaters, and many bacterial species with a role in manganese bioremediation have been identified over several decades. Using K medium with Mn(II), a bacterial consortium was obtained from water sample collected from a mine drainage in southeastern Brazil (Minas Gerais, Brazil), and the isolates were identified as Klebsiella oxytoca using both biochemical and 16S rRNA phylogenetic analysis. Among such isolates, sample SA8 was tested for its manganese removal capability during a week-long experiment in K medium amended with manganese sulfate. Growth of isolate SA8 produced an increase in pH concomitantly to Mn(II) removal, which was not observed in the abiotic control. Manganese removal occurred through oxidation as confirmed by reaction with leucoberbelin blue, and therefore, a new potential application for K. oxytoca in mine water bioremediation is proposed.  相似文献   

Development and evaluation of a standard area diagram set for the severity of phomopsis leaf blight on eggplant     

Kamila Câmara Correia  João Victor Jansen de Queiroz  Ricardo Brainer Martins  Alessandro Nicoli  Emerson Medeiros Del Ponte  Sami Jorge Michereff 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(2):269-276

A standard area diagram set (SADs) with eight severity values ranging from 0.5 to 32% was evaluated as a tool to improve the accuracy of the estimates of Phomopsis leaf blight severity on eggplant by ten inexperienced raters. A first assessment in a 50-leaf dataset of digital images was performed unaided (UN, no SAD). Two further aided (A) assessments, conducted two (A1) and four weeks after the first (A2) unaided one were performed. The precision of the estimates, as indicated by the Pearson’s correlation coefficient (r), improved significantly when using the aid (A1: r = 0.96 and A2: r = 0.96) compared to unaided (UN: r = 0.79). However, the generalized bias (C _b) was not significantly affected because the unaided estimates were already quite accurate (C _b = 0.93). The overall concordance (ρ _c) was significantly improved due to the large gains in precision when using the SADs. The raters’ estimates were more uniform (ρ _c > 0.9) when using the aid, with progressive gains in the reliability of the estimates among them, indicated by the concordance correlation coefficient statistics. The SADs proposed in this study will be useful in severity estimation during field work involving multiple raters, especially as use of the SADs results in less variable estimates, improving accuracy and reliability among raters.  相似文献   

Evaluation of sustainability in Eastern Amazon under proambiente program     

J. S. R. Oliveira  O. R. Kato  T. F. Oliveira  J. C. B. Queiroz 《Agroforestry Systems》2010,78(3):185-191

This study evaluates the sustainability of the innovative practices of smallholders who have extended their traditional farming and backyard gardening to other production parcels, such as agroforest systems in Eastern Amazon, Northeast Pará, under the PROAMBIENTE Program at Capim River Pole. According to these smallholders, these practices have assured food supplies and yields with the inclusion into the consumer market through produce diversity obtained by agroforest arrangement and increased purchase of material goods to the system. The smallholders’ perceptions also permit the evaluation of the sustainability of their experiences through the “Amoeba” method, which consolidates economic, social, cultural, and environmental indicators.  相似文献   

Assessment of Orchidometry and Scrotal Circumference in Coatis (Nasua nasua)     

JPAF Queiroz  FFPC Barros  GL Lima  TS Castelo  CIA Freitas  AR Silva 《Reproduction in domestic animals》2010,45(6):e382-e386

This study describes the morphometry of the testicles of captive coatis (Nasua nasua) and verifies the existence of correlations between the testicles and the semen producing characteristics. Twelve ejaculates were obtained from five adult specimens that were restraint by anaesthesia. Visual and morphometric evaluation of the external genitalia was performed by using a caliper. The animals presented a scrotal circumference of 9.4 ± 0.7 cm. The right testicle presented a width of 1.7 ± 0.2 cm, length of 2.6 ± 0.2 cm and volume of 3.9 ± 1.1 cm³. The left testicle presented a width of 1.7 ± 0.1 cm, length of 2.5 ± 0.3 cm and volume of 3.6 ± 0.7 cm³. No significant correlations were obtained between the scrotal circumference or total testicular volume and the semen producing characteristics (volume and sperm concentration). For the first time in this field, this study presents an important contribution to the knowledge of the reproductive morphophysiology of coatis.  相似文献   

Effect of sewage sludge and sugarcane bagasse biochar on soil properties and sugar beet production     

Barbara Samartini Queiroz ALVES  Katherin Prissila Sevilla ZELAYA  Fernando COLEN  Ledivan Almeida FRAZ&#;O  Alfredo NAPOLI  Sanjai J. PARIKH  Luiz Arnaldo FERNANDES 《土壤圈》2021,31(4):572-582

Recently, biochar has shown to be an alternative to waste disposal and a source of nutrients, acting as a soil amendment. The effects of two types of biochar on soil properties and sugar beet production as well as potential for carbon (C) sequestration were evaluated:biochar produced from sewage sludge (SB) and biochar produced from a 1:1 mixture of sewage sludge and sugarcane bagasse (MB). A greenhouse pot experiment was conducted using a sandy loam soil from the Brazilian savanna under treatments of MB applications at 2.5%, 5.0%, 7.5%, and 10.0%, SB application at 5.0%, and a conventional fertilization (CF) using lime and mineral fertilizers, with no fertilization as a control. After incubation for 45 d, seedlings were transplanted into each pot and cultivated for 55 d. Biochar characterization showed that pyrolysis reduced the biomass volume drastically, but concentrated the trace elements per unit of biochar weight. The MB treatments increased soil total C (by 27.8%) and pH (by 0.6), reduced the concentrations of nutrients, except for potassium (K), and chromium (Cr), and did not significantly alter lead (Pb) and cadmium (Cd) concentrations. Results of stable isotopes showed that all biochar treatments increased the total soil C stock and stability, suggesting a potential for application in C sequestration, and improved overall soil fertility. However, the biochar treatments also increased the concentrations of trace elements in the soil and plants. The sugar beet yields at 10.0% MB and 5.0% SB corresponded to 55% and 29% of the yield obtained in the CF treatment, respectively. These results may be due to biochar nutrients not being bioavailable when required by plants or to biochar nutrient adsorption.  相似文献   

In vitro Bioaccessibility of Proteins,Phenolics, Flavonoids and Antioxidant Activity of Amaranthus viridis     

Silva  Alisson David  Ávila  Suelen  Küster  Renata Tulio  dos Santos  Mayara Padovan  Grassi  Marco Tadeu  de Queiroz Pereira Pinto  Christiane  Miguel  Obdulio Gomes  Ferreira  Sila Mary Rodrigues 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):478-486

Plant Foods for Human Nutrition - The aim of this study was to investigate the chemical composition, mineral content and report the effect of gastrointestinal digestion on the proteins, phenolics,...  相似文献   

Molecular characterization of encephalitic bovine listeriosis from southern Brazil     

Selwyn A. Headley  Juliana T. T. Fritzen  Gustavo R. Queiroz  Rodrigo A. M. Oliveira  Alice F. Alfieri  Giovana W. Di Santis  Júlio A. N. Lisbôa  Amauri A. Alfieri 《Tropical animal health and production》2014,46(1):19-25

  相似文献   

Pharmacopuncture Versus Acepromazine in Stress Responses of Horses During Road Transport     

Tatianne Leme Oliveira Santos Godoi  Julia Dias Villas-Boas  Norma Aparecida dos Santos Almeida  Pablo Ignacio Trigo  Fernando Queiroz de Almeida  Magda Alves de Medeiros 《Journal of Equine Veterinary Science》2014

Acupuncture has been shown to have the beneficial effect of reducing stress responses in animals and humans. Pharmacopuncture is the injection of subclinical doses of drugs into acupoints to give therapeutic results without side effects. This study compared the effects of injecting the usual dose of acepromazine (ACP; 0.1 mg/kg, intramuscularly [I.M.]) with those of pharmacopuncture (1/10 ACP dose at the governing vessel 1 [GV 1] acupoint) on the stress responses of healthy horses undergoing road transport for 2.5 hours. Four different treatments were applied immediately before loading, with 8 animals/treatment: injection of saline or ACP (0.1 mg/kg, I.M.) at the base of the neck; and injection of saline or 1/10 ACP (0.01 mg/kg) at the GV 1 acupoint. The road transport increased heart rate (HR), respiratory rate, body temperature, and serum cortisol of the untreated horses (injected with saline at the base of the neck). Pharmacopuncture at GV 1 reduced the average HR and transport-induced increase in HR at unloading, without changing the other variables. On the other hand, ACP (0.1 mg/kg) produced significant sedation and reduced the transport-induced increase in respiratory rate but without preventing the stress-induced increase of cortisol. Other acupuncture points and drugs should be tested to verify the beneficial effect of this therapy to reduce stress in horses during road transport.  相似文献