全文获取类型
| 收费全文 | 108篇 |
| 免费 | 7篇 |
专业分类
| 林业 | 7篇 |
| 农学 | 3篇 |
| 13篇 | |
| 综合类 | 6篇 |
| 农作物 | 10篇 |
| 水产渔业 | 15篇 |
| 畜牧兽医 | 53篇 |
| 园艺 | 1篇 |
| 植物保护 | 7篇 |
出版年
| 2023年 | 2篇 |
| 2022年 | 5篇 |
| 2021年 | 9篇 |
| 2020年 | 11篇 |
| 2019年 | 4篇 |
| 2018年 | 3篇 |
| 2017年 | 7篇 |
| 2016年 | 8篇 |
| 2015年 | 1篇 |
| 2014年 | 9篇 |
| 2013年 | 7篇 |
| 2012年 | 4篇 |
| 2011年 | 9篇 |
| 2010年 | 5篇 |
| 2009年 | 2篇 |
| 2008年 | 4篇 |
| 2007年 | 9篇 |
| 2006年 | 2篇 |
| 2005年 | 1篇 |
| 2003年 | 3篇 |
| 2002年 | 2篇 |
| 2000年 | 2篇 |
| 1998年 | 3篇 |
| 1993年 | 1篇 |
| 1990年 | 1篇 |
| 1974年 | 1篇 |
排序方式： 共有115条查询结果，搜索用时 140 毫秒
41.
Cardoso SR Queiroz LB Goulart VA Mourão GB Benedetti E Goulart LR 《Research in veterinary science》2011,91(3):e107-e112
Candidate genes have been associated with milk production in bovines, such as the diacylglycerol O-acyltransferase 1 (DGAT1) and leptin (LEP); however, they have not been simultaneously investigated nor have been evaluated in the Brazilian Girolando breed (Gir × Holstein, backcrossed to Holstein). Our aim was to determine the influence of fat-related genes, DGAT1 and LEP, and their polymorphisms on performance traits of milk production in the Girolando breed. Results indicated that the K allele of the DGAT1 gene showed a significant association with total and average daily milk production with additive effect. The LEP gene showed that the A allele and its homozygote are highly prevalent and almost fixed in this population and may have been favorably selected during backcrossing for the origin of this breed. The important impact of the K allele of the DGAT1 gene on milk production corroborates the initiative of performing marker-assisted selections with this gene in breeding programs of the Girolando breed. 相似文献
42.
The present investigation was intended to show a different immunohistochemical profile of matrix metalloproteinase-2 and Tissue
inhibitor metalloproteinase-2 in bovine uteri with adenomyosis during follicular phase. Uterine samples of 32 cows in reproductive
age were taken from the medial third of one of the uterine horns and grouped according to the adenomyosis degree (superficial
and deep). Tissue sections (4 μm) were incubated overnight at 4°C with monoclonal antibody for matrix metalloproteinase-2
and Tissue inhibitor metalloproteinase-2. Staining intensities were evaluated in the luminal epithelium, ectopic and dystopic
endometrial tissue (stroma, capillaries and glands), endometrial-myometrial border, myometrium, myometrial vessels (middle
tunic and endothelium). The matrix metalloproteinase-2 expression was higher for deep adenomyosis samples, showing a differential
mean reactivity in superficial endometrium, myometrial vessels, myometrium adjacent to adenomyotic focus and endometrial-myometrial
border (P < 0.05). Moreover, matrix metalloproteinase-2 expression was higher in deep adenomyosis samples than that of Tissue inhibitor
metalloproteinase-2 in almost all uterine structures analyzed (except for the endometrial and myometrial vessels and endometrial-myometrial
border). The opposite was observed in the follicular phase, for both normal specimens and with superficial adenomyosis, where
Tissue inhibitor metalloproteinase-2 expression was higher than that of matrix metalloproteinase-2. In conclusion, a differential
pattern of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 was observed in cow uteri with adenomyosis. 相似文献
43.
In developing countries such as Brazil, where canine rabies is still a considerable problem, samples from wildlife species are infrequently collected and submitted for screening for rabies. A collaborative study was established involving environmental biologists and veterinarians for rabies epidemiological research in a specific ecological area located at the Sao Paulo State, Brazil. The wild animals’ brains are required to be collected without skull damage because the skull’s measurements are important in the identification of the captured animal species. For this purpose, samples from bats and small mammals were collected using an aspiration method by inserting a plastic pipette into the brain through the magnum foramen. While there is a progressive increase in the use of the plastic pipette technique in various studies undertaken, it is also appreciated that this method could foster collaborative research between wildlife scientists and rabies epidemiologists thus improving rabies surveillance. 相似文献
44.
Queiroz TM Machado NT Furtado FF Oliveira-Filho AA Alustau MC Figueiredo CS Miranda GE Barbosa-Filho JM Braga VA Medeiros IA 《Marine drugs》2011,9(10):2075-2088
This study aimed to investigate the cardiovascular effects elicited by Dictyota pulchella, a brown alga, using in vivo and in vitro approaches. In normotensive conscious rats, CH(2)Cl(2)/MeOH Extract (CME, 5, 10, 20 and 40 mg/kg) from Dictyota pulchella produced dose-dependent hypotension (-4 ± 1; -8 ± 2; -53 ± 8 and -63 ± 3 mmHg) and bradycardia (-8 ± 6; -17 ± 11; -257 ± 36 and -285 ± 27 b.p.m.). In addition, CME and Hexane/EtOAc Phase (HEP) (0.01-300 μg/mL) from Dictyota pulchella induced a concentration-dependent relaxation in phenylephrine (Phe, 1 μM)-pre-contracted mesenteric artery rings. The vasorelaxant effect was not modified by the removal of the vascular endothelium or pre-incubation with KCl (20 mM), tetraethylammonium (TEA, 3 mM) or tromboxane A(2) agonist U-46619 (100 nM). Furthermore, CME and HEP reversed CaCl(2)-induced vascular contractions. These results suggest that both CME and HEP act on the voltage-operated calcium channel in order to produce vasorelaxation. In addition, CME induced vasodilatation after the vessels have been pre-contracted with L-type Ca(2+) channel agonist (Bay K 8644, 200 nM). Taken together, our data show that CME induces hypotension and bradycardia in vivo and that both CME and HEP induce endothelium-independent vasodilatation in vitro that seems to involve the inhibition of the Ca(2+) influx through blockade of voltage-operated calcium channels. 相似文献
45.
Lívia Ribeiro de Souza Kay Knöller Ana Cláudia Queiroz Ladeira 《Journal of Soils and Sediments》2016,16(7):1986-1994
Purpose
The present study investigated lake and river sediments affected by metals from an acid mine drainage (AMD) from a former uranium mine. The role of bacterial sulfate reduction in the immobilization of contaminants was evaluated, and the analyses of acid volatile sulfide (AVS) and sequential extraction were performed. Consequently, the potential mobility and bioavailability of contaminants were established.Materials and methods
Sulfur isotopic fractionation (δ34S), AVS, and sequential extraction procedure were used to assess the sulfate bacterial reduction and the availability of contaminants in the environment at six sampling stations.Results and discussion
The δ34S indicated that bacterial reduction is a key process in the natural attenuation of contamination in the Águas Claras reservoir, precipitating metal sulfides. According to the USEPA criteria, adverse biological effects are expected for sample S1 (inside the reservoir) which is likely to be toxic, while for sediment S4 (in the river), the toxicity is uncertain. The other samples were classified as non-toxic, likely because of the decreased solubility of zinc sulfide. A decrease in the concentration of the contaminants downstream of the reservoir was observed. The predominance of U (0.4 %) in the labile fraction and the elevated concentrations of Zn (0.5 %) and Mn (0.7 %) in the sediments inside the reservoir raises concerns regarding the availability of these contaminants in the environment.Conclusions
The main environmental impact appears to be concentrated in the Águas Claras reservoir, whereas the Antas creek does not seem to be affected by the AMD process. Although the bacterial sulfate reduction is effective in its production of sulfides capable of immobilizing the contaminants, the presence of Zn and U in the labile and reducible fraction is a matter of concern due to its long-term bioavailability. Thus, continuous monitoring of the redox potential of the waters and sediments, mainly in the reservoir, is recommended in order to assess and possibly prevent later dissolution of sequestered contaminants.46.
de Souza Junior Francisco Jorge Carlos dos Santos Vieira Willie Anderson Veloso Josiene Silva Duarte Ingrid Gomes Amaral Ana Gabriele Gurgel Laranjeira Delson Balbino Valdir De Queiroz Doyle Vinson P. Câmara Marcos Paz Saraiva 《European journal of plant pathology / European Foundation for Plant Pathology》2021,160(3):745-751
European Journal of Plant Pathology - Bauhinia forficata is an important plant commonly used in traditional medicine, and as an ornamental plant in parks and gardens. Its use as an ornamental plant... 相似文献
47.
Barbarella Matos de Macchi Farlen José Bebber Miranda Fernanda Silva de Souza Eulógio Carlos Queiroz de Carvalho Ant?nio Peixoto Albernaz José Luiz Martins do Nascimento Renato Augusto DaMatta 《Veterinary research》2013,44(1):8
Malaria is a serious infectious disease caused by parasites of the Plasmodium genus that affect different vertebrate hosts. Severe malaria leads to host death and involves different pathophysiological phenomena such as anemia, thrombocytopenia and inflammation. Nitric oxide (NO) is an important effector molecule in this disease, but little is known about its role in avian malaria models. Plasmodium gallinaceum- infected chickens were treated with aminoguanidine (AG), an inhibitor of inducible nitric oxide synthase, to observe the role of NO in the pathogenesis of this avian model. AG increased the survival of chickens, but also induced higher parasitemia. Treated chickens demonstrated reduced anemia and thrombocytopenia. Moreover, erythrocytes at different stages of maturation, heterophils, monocytes and thrombocytes were infected by Plasmodium gallinaceum and animals presented a generalized leucopenia. Activated leukocytes and thrombocytes with elongated double nuclei were observed in chickens with higher parasitemia; however, eosinophils were not involved in the infection. AG reduced levels of hemozoin in the spleen and liver, indicating lower inflammation. Taken together, the results suggest that AG reduced anemia, thrombocytopenia and inflammation, explaining the greater survival rate of the treated chickens. 相似文献
48.
VRP Barros AYP Cavalcante TJS Macedo RS Barberino TLB Lins BB Gouveia VG Menezes MAA Queiroz VR Araújo RC Palheta Jr MCP Leite MHT Matos 《Reproduction in domestic animals》2013,48(6):1025-1033
The expression of melatonin type 1 (MT1) and FSH (FSHR) receptors in caprine ovaries and the effects of these hormones on the in vitro development of isolated pre‐antral follicles were evaluated. Follicles (≤200 μm) were cultured for 12 days in α‐MEM (control) or melatonin (100 or 1000 pg/ml) or sequential melatonin medium (100 pg/ml: from day 0 to day 6; 1000 pg/ml: from day 6 to day 12; experiment 1) and in control or sequential FSH (100 ng/ml from day 0 to day 6; 500 ng/ml from day 6 to day 12) or sequential melatonin or this latter plus sequential FSH (experiment 2). MT1 and FSHR expressions were observed in granulosa cells from secondary and antral follicles. The oocytes from primordial and primary follicles also express FSHR. Sequential melatonin increased the percentage of normal follicles and oocyte recovery compared with the control or melatonin (1000 pg/ml) at day 12. In experiment 2, all the treatments increased the normal follicles and growth compared with the control. In conclusion, this study demonstrated the presence of MT1 and FSHR in caprine ovaries. The addition of increased concentrations of melatonin (sequential medium) or FSH can be used to promote the in vitro development of caprine pre‐antral follicles. 相似文献
49.
50.
F. M. Paterno M. E. Buzanskas W. Koury Filho R. B. Lôbo S. A. Queiroz 《Tropical animal health and production》2017,49(3):467-473
The aims of our study were to estimate genetic parameters for body weight and visual scores and to evaluate their inclusion as selection criteria in the Nelore breeding program in Brazil. The traits studied were the body weight adjusted to 210 (W210) and to 450 (W450) days of age and visual scores for body structure, finishing precocity, and muscling evaluated at weaning (BSW, FPW, and MSW) and yearling (BSY, FPY, and MSY) ages. A total of 33,242, 26,259, 23,075, and 26,057 observations were considered to analyze W210, W450, and visual scores at weaning and yearling. The significant (P?<?0.05) fixed effects for all traits were farm, birth season, birth year, sex, and management group. Single-trait analyses were performed to define the most fitting model to our data using the average information restricted maximum likelihood algorithm, for weaning traits. Subsequently, these models were used in single- and two-trait analyses considering the Bayesian inference algorithm. Two-trait Bayesian analyses resulted in average direct heritability estimates for BSW, FPW, MSW, W210, BSY, FPY, MSY, and W450 of 0.28, 0.30, 0.27, 0.28, 0.40, 0.44, 0.39, and 0.50, respectively. Genetic correlations varied from 0.40 to 0.96. Benefits to animal performance can best be achieved by considering body structure, finishing precocity, and muscling as selection criteria in the Nelore breeding programs. The decision to use visual scores measured at weaning should be considered in order to decrease generation interval and assist pre-selecting individuals, expecting carcass improvements in the future progeny. 相似文献