Farmers’ willingness to pay for community integrated pest management training in Nepal     

Kishor Atreya 《Agriculture and Human Values》2007,24(3):399-409

The concept of community integrated pest management (IPM), which is well developed in Indonesia and Vietnam, was recently introduced in Nepal. However, it has not been widely practiced, due mainly to lack of financial and technical support. This study determined an individual’s willingness to pay (WTP) for community IPM training. Determinants of WTP were identified; and sample average estimates, opportunity costs of training, and probability values were used to estimate WTP for a group of households. Estimated WTP revealed that individuals were in favor of community IPM, hence it could be implemented with the support of local villagers. Community IPM demand functions showed that individuals’ knowledge and awareness of pesticide pollution are crucial for implementation. The annual welfare gained by providing five days community IPM training was calculated to be US $25.23 per household. Kishor Atreya has a Master’s degree in Environmental Science and is currently working at Alternative Development and Research Center (ADRC) as Research Director. ADRC – Nepal is a national non-governmental organization dedicated to interdisciplinary research for alternative development. His research interests are in development of agri-environmental indicators, land and water quality, land use change and degradation, agricultural intensification and environment, and economic valuation of environmental pollution.  相似文献   

Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.)     

H. Kotresh  B. Fakrudin  S. M. Punnuri  B. K. Rajkumar  M. Thudi  H. Paramesh  H. Lohithswa  M. S. Kuruvinashetti 《Euphytica》2006,149(1-2):113-120

Summary Fusarium wilt (Fusarium udum Butler) is a soil borne disease of pigeonpea which causes substantial yield losses. The disease can occur at any stage of plant development, from the young seedling to the pod filling stage. Though resistance is simply inherited, transfer to locally adapted cultivars has been difficult due to linkage drag and difficulty in accurate phenotyping, except in sick plots. An attempt was made to identify RAPD markers associated with wilt phenotype by using F2 populations derived from contrasting parents; GSl (susceptible) ‘ICPL87119 (resistant) and GS1’ ICP8863 (resistant). Parents and F₂s were grown in a national _Fusarium sick-plot at Gulbarga, India and phenotyped as resistant or susceptible during the entire crop growth period. In both the crosses, resistance to wilt segregated as a monogenic dominant character. DNA samples extracted from sick plot grown, early seedling stage plants of parents and 254 F₂ plants of GS1 × ICPL87119 were held separately for marker identification. PCR reactions using 340 random decamer primers with genomic DNA of parents resulted in detection of 45 polymorphic amplicons from 39 primers. PCR testing of bulked DNA from subsets of resistant and susceptible plants revealed the presence of two amplicons at 704 bp and 500 bp (OPM03704 and OPAC11500) with susceptibility. Analysis of individual F₂ plants showed a segregation ratio of 3: 1 for the presence: absence of the amplicon in both crosses. Considering the wilt reaction and susceptibility-linked RAPD marker, it was possible to deduce genotype of every F₂ plant and the genotypic ratio for wilt reaction was 1RR: 2Rr: 1rr, as expected.  相似文献   

Gharials (Gavialis gangeticus) in Bardiya National Park,Nepal: Population,habitat and threats     

Ashish Bashyal  Sandeep Shrestha  Kishor P. Luitel  Bhupendra P. Yadav  Bed Khadka  Jeffrey W. Lang  Llewellyn D. Densmore 《水产资源保护:海洋与淡水生态系统》2021,31(9):2594-2602

1. The Critically Endangered gharial (Gavialis gangeticus) residing within Bardiya National Park (BNP) in Nepal constitutes the sixth major sub-population of this unique lineage; however, this population is not well studied. A 46 km protected stretch of the Babai River and a 60 km stretch of the Karnali River in and around BNP were surveyed for gharials in early 2017 and in 2019.
2. Gharial counts in 2017 – 17 from the Babai and one from the Karnali rivers – consisted of 10 adults, five sub-adults and three juveniles. In 2019, 19 gharials were counted – 18 for the Babai and one for the Karnali – comprising 11 adults, six sub-adults and two juveniles. In the Babai River within BNP, four breeding groups were identified. Gharials in BNP prefer sandy vs. rocky banks for basking and completely avoid sand-grass and clay banks. Habitat preference did not vary with size class.
3. Protected stretches of both rivers inside BNP (76 km) have resident gharial, intact habitats and few human threats. In contrast, the unprotected stretch of the Karnali (30 km) is threatened by boulder quarrying, sand mining and unlicensed fishing, and is avoided by gharials despite the availability of habitat.
4. This study established baseline data, including indices of population size, distribution and habitat preferences, and documented resident gharials that are breeding in the Babai River in BNP. The conservation of this Babai population is crucial for the species’ survival in BNP.
5. Based on this study, we recommend the following conservation actions on the Babai stretch: (a) determine hatching success; (b) assess juvenile recruitment; (c) consider supplementation by releasing captives; (d) study riverine features important as baselines; (e) accommodate upstream movements on the protected Babai stretch; and (f) protect the downstream Babai outside the boundary of the BNP.

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Applications of reduced tillage in hills of central Nepal     

Kishor Atreya  Subodh Sharma  Roshan Man Bajracharya  Neeranjan Prasad Rajbhandari 《Soil & Tillage Research》2006,88(1-2):16-29

Conventional tillage practices on steep and fragile landscape of Himalayan hills result in significant loss of topsoil during rainy season. Soil erosion in Nepal mid-hills is the most critical during pre-monsoon season. Many reviews argue that reduced tillage could be an option to tackle this problem. However, very few field experiments to evaluate reduced tillage systems have to date been conducted in this region. Thus, a field experiment was initiated in factorial randomized complete block design on acidic sandy loam soil (Lithic Dystochrept) during the summer season of 2001 at Kathmandu University (1500 masl) to assess the effects of tillage and cropping patterns on soil and nutrient losses, crop yield and soil fertility. Two main treatments viz. conventional and reduced till, and two sub-treatments viz. sole maize (Zea mays) and maize + soybean (Glycine max) were considered. Soil organic carbon (OC), total nitrogen (N), plant available phosphorus (P) and exchangeable potassium (K) were determined for the original soil and eroded sediment using standard methods. Two years of data indicated annual soil and nutrient losses to be significantly lowered by reduced till as compared to conventional till. Total annual soil loss from conventional and reduced till was 16.6 and 11.1 Mg/ha, respectively. Similarly, annual nutrient losses associated with the eroded sediment were 188 kg OC/ha, 18.8 kg N/ha, <1 kg P/ha and 3.8 kg K/ha for conventional till and 126 kg OC/ha, 11.8 kg N/ha, <1 kg P/ha and 2.4 kg K/ha for reduced till. Soil OC and N losses were significantly higher in conventional till and this may be one of the major causes of fertility depletion in the Nepalese hills. Soil chemical properties did not differ due to tillage and cropping systems; however, over years pH, N and P were increased irrespective of treatments. Although treatments were at par for maize grain yield, conventional till + soybean produced highest grain yield (4.0 Mg/ha) followed by reduced till + soybean (3.9 Mg/ha) and conventional till sole maize (3.8 Mg/ha). Mixed cropping of legumes and maize do not help conserve soil and nutrient loss in hills of central Nepal. Thus, reduced till could be a viable option for minimizing soil and nutrient losses without sacrificing economic yields in central hills of Nepal.  相似文献   

Association of polymorphism in the prolactin promoter and egg quality traits in laying hens     

Bhattacharya TK  Chatterjee RN  Sharma RP  Rajkumar U  Niranjan M  Reddy BL 《British poultry science》2011,52(5):551-557

1. Single strand conformation polymorphism analysis and DNA sequencing was performed in White Leghorn hens to explore the polymorphisms present in the promoter of the prolactin gene. The effects of different genotypes on egg production and quality traits were determined, and expression of the prolactin gene in different genotypes was quantified by real time-PCR.

2. Five genotypes and four alleles at each of two Fragments of the promoter were found, of which the FG genotype in Fragment 1 and the PQ genotype in Fragment 2 were the most predominant genotypes.

3. The genotypes of Fragment 1 had significant effects (P?

4. Prolactin expression in the genotypes of Fragment 1 differed significantly and GH genotyped birds had the highest level of expression. The genotypes of Fragment 2 did not show any significant differences of expression.

5. It was concluded that the prolactin gene promoter was highly polymorphic, and had significant effects on egg quality traits in White Leghorn hens.  相似文献   

Genome organization in coffee as revealed by EST PCRRFLP,SNPs and SSR analysis     

Manoj Kumar Mishra  Patrizia Tornincasa  Barbara De Nardi  Elisa Asquini  René Dreos  Lorenzo Del Terra  Rajkumar Rathinavelu  Paola Rovelli  Alberto Pallavicini  Giorgio Graziosi 《Journal of Crop Science and Biotechnology》2011,14(1):25-37

An EST-based PCR-RFLP method was employed to gain insight into genome organization in eight allopolyploid Coffea arabica cultivars and seven diploid coffee species. The PCR-amplified products at 19 EST loci were digested with 46 different restriction enzymes and size fractioned in agarose gels. Most often, the sum of the fragments length was double or more than the PCR product. In arabica, this condition could be explained by assuming the presence of duplicated loci in paralogous chromosomes and this was supported by considerable evidence of multiple loci SSR amplification. Based on the RFLP analysis, 12 EST loci were polymorphic. The level of polymorphism was higher in different species compared to the arabica varieties. Sequencing of the amplified products revealed a SNP frequency of 0.021 among diploid species and of 0.007 among arabica varieties. We propose that the involvement of two genomes in C. arabica maintains a residual level of heterozygosity in the form of paralogous chromosomes, while the self-fertilization in this species tends to drive of homozygosity. The heterozygosity of paralogous chromosomes in arabica creates valuable polymorphism essential for species diversity and survival in various ecological niches, while self-fertility tends to preserve in homozygosity many genes of functional significance.  相似文献   

Partitioning of phosphorus in different height isolines of winter wheat     

Kishor Sherchand  Gary M. Paulsen 《Journal of plant nutrition》2013,36(12):1147-1160

Wheat (Triticum aestivum L.) grain yields and composition are influenced greatly by P and its interactions with other nutrients. Because most P in grain, like N, is remobilized from vegetative parts, high harvest index of dwarf wheats may adversely influence partitioning of P to grain. Isogenic tall, single semidwarf, and double dwarf ‘Pawnee’ wheat lines were used to characterize P partitioning, P nutrition before and after anthesis, and relationship between P and N. Plants were grown in hydroponic cultures with three P levels; the first study determined P, N, and dry matter accumulation from anthesis to maturity, the second study determined the effect of withholding P after anthesis on P, N, and dry matter partitioning during grain development. Plants continued to absorb P when it was available after anthesis, but grain growth was influenced more by P nutrition before anthesis than after anthesis. Leaf blades, sheaths, and stems were major sources of P for grain growth when the nutrient was withheld and major sites of P accumulation when the nutrient was supplied after anthesis. Content of N in plants was favored by ample P levels. Dwarfing genes increased harvest index, but had little effect on P partitioning because of differences in dry matter accumulation.  相似文献   

Effects of Treatment for Anestrus in Water Buffaloes with PGF2α and GnRH in Comparison with Vitamin-Mineral Supplement,and Some Factors Influencing Treatment Effects     

Bhuminand DEVKOTA  Toshihiko NAKAO  Kosaku KOBAYASHI  Hiroshi SATO  Shyam Kishor SAH  Dinesh Kumar SINGH  Ishwori Prasad DHAKAL  Norio YAMAGISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1623-1627

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Response of upland cotton (<Emphasis Type="Italic">G.hirsutum</Emphasis> L.) genotypes to drought stress using drought tolerance indices     

Chandrakant?SinghEmail author  Vijay?Kumar  Indivar?Prasad  Vishal?R.?Patil  B.?K.?Rajkumar 《Journal of Crop Science and Biotechnology》2016,19(1):53-59

Drought tolerance as such is often not considered to be an independent trait by plant breeders. The objective of this study was to evaluate eight drought tolerance indices, namely stress susceptibility index (SSI), yield stability index (YSI), yield reduction ratio (Yr), yield index (YI), tolerance index (TOL), mean productivity (MP), geometric mean productivity (GMP), and stress tolerance index (STI) in upland cotton (G. hirsutum L.) genotypes. For this purpose, 16 genotypes were sampled during the 2013-2014 growing seasons under both normal and drought-stress field conditions at the Main Cotton Research Station of Navsari Agricultural University, Surat, India. The drought tolerance indices were calculated based on seed cotton yield under drought stress and non-stress conditions. Mean comparison of drought tolerance indices and seed cotton yield validated the significant influences of drought stress on yield as well as significant differences among genotypes. Results of calculated correlation coefficients and multivariate analyses showed that GMP, MP and STI indices were able to discriminate drought-sensitive and tolerant genotypes. Cluster analysis using the drought-tolerance indices divided the 16 genotypes into tolerant and susceptible groups. Two genotypes, G.Cot.16 × H-1353/10 and H-1353/10 × G.Cot.16 gave good yield response under drought conditions leading to their stability during water stress conditions. Based on multivariate analyses using the indices individually or in combinations, it was possible to identify the most yield-stable genotypes across the environments. Overall, we concluded that GMP, MP and STI indices can be efficiently exploited not only for screening drought tolerance but also to identify superior genotypessuitable for both stress and non-stress field conditions.  相似文献   

Role of foresters' perspectives in orienting agroforestry programmes     

Kamal Kishor Sood  C. Paul Mitchell 《Forest Policy and Economics》2009,11(4):213-220

The foresters' perspectives of agroforestry is an important aspect that can influence strategies concerned with agroforestry uptake by the farmers. Keeping this in view the empirical studies of foresters' perspective of agroforestry have gained considerable importance. Present study, therefore, investigates foresters' perspectives on different aspects related to agroforestry. This study was conducted in Mandi district located in Indian Western Himalayan province of Himachal Pradesh. Not only the traditional forest management but also agroforestry training of foresters has remained oriented towards learning silvi-technical (plantation management and nursery management) rather than extension (social) and agricultural aspects. Consequently, there was a felt need amongst foresters for training in extension and agricultural aspects for effective implementation of agroforestry programmes. This provides a window of opportunity for re-orienting their training curriculum towards learning extension (social) and agricultural besides the silvi-technical skills. For effective implementation of agroforestry programmes the manpower requirement, particularly that of extension specialist and well trained agroforesters, of the department needs to be estimated and strategy should be devised to minimise political interference. The study emphasises a need for interaction between foresters and farmers, mechanism for better co-ordination with other departments and absolving agroforesters of their policing role for effective planning and implementation of agroforestry programmes. There were significant variations in the opinion of forestry staff about the effectiveness of various strategies to meet farmers tree-based needs, increase tree cover in the state and expected plantation survival rate. There were significant variations in the opinion of foresters about the different factors discouraging private tree growing but ‘restriction on felling green trees growing on private land and selling them in the market’ was viewed as the most important factor. “Provision of incentives to the villagers for tree growing on private land” was the major factor from the foresters' perspective that will encourage tree growing on private land. As there was a lack of congruence of foresters' perception on majority of issues pertaining to agroforestry, a need to incorporate the uniform understanding of issues pertaining to agroforestry amongst the foresters was emphasised. This study also identifies a need to investigate the perspectives staff of agriculture, horticulture departments for better planning and execution of agroforestry programmes, and their uptake by the farmers.  相似文献