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11.
Sumaira Qutab Rizwan Rasheed M. Arslan Ashraf Iqbal Hussain Nudrat Aisha Akram 《Archives of Agronomy and Soil Science》2017,63(13):1900-1911
The effects of selenium (Se) cadmium (Cd) interactions on plant growth and metabolism are not fully clear. In the present study, we assessed whether Se could alleviate the toxic effects of Cd on growth and metabolism of maize. Seeds of maize variety FH-985 were sown in pots filled with sand treated with CdCl2 (0, 50 and 100 µM) and Se (0, 2 and 4 mg L?1) through Hoagland’s nutrient solution. Low Se (2 mg L?1) increased germination percentage and rate, while high Se (4 mg L?1) increased fresh and dry biomass under Cd stress. Interestingly, all Se concentrations were effective in alleviating the toxic effects of Cd on photosynthetic pigments, whereas higher Se mitigated the Cd-induced oxidative stress and increased flavonoids both in the shoots and roots while phenolics in the roots. The results demonstrated that root zone Se altered tissue-specific primary metabolism in maize. Furthermore, low Se mitigated the Cd-induced decrease in total proteins in the root. Overall, Se-mediated decrease in the oxidative stress in the shoots while increase of secondary metabolites in the roots helped the plants to grow faster at early growth stage and caused increase in the biomass under different Cd regimes. 相似文献
12.
Oluwaseun Serah Iyasere David Oluwatowo Adeuyi Rasheed Adebayo Alade Samuel Olutunde Durosaro Mathew Wheto Olajide James Ogunbanjo Lucky Ebhodage Oyakhilome Samuel Emmanuel James Olamitibo Daramola 《Reproduction in domestic animals》2020,55(5):567-573
To date, there is no reported standard mating ratio for Nigerian native chickens despite their large population in the country. This study investigated the effect of mating ratio on behaviour, semen quality and level of stress. Sixty-three (9 cocks and 54 hens) sexually matured birds were randomly assigned to three mating ratios (MR) of 1 cock to 3 hens (1C:3H), 1 cock to 6 hens (1C:6H) and 1 cock to 9 hens (1C:9H), and each MR was replicated three times. Each cock spent 2 weeks in a particular MR before it was moved to another. By the 6th week, all cocks had experienced the three mating ratios. Behaviour of the hens and sexual behaviour of the cocks were observed in the morning and evening, respectively, for 6 weeks. At the end of 2nd week in each MR, semen samples were collected and evaluated for motility, morphology, liveability and concentration, and blood samples were also taken from the cocks to determine the heterophil/lymphocyte (H:L) ratio, an indicator of stress. There was also no significant effect of mating ratio on the fertility of eggs and hatchability of the chicks. The number of hen–hen aggression instances, frequency of visits to the nest box and duration of nesting was not influenced by the mating ratio. However, the duration of nest seeking was higher at 1C:3H and lower at 1C:9H. A mating ratio of 1C:9H is therefore recommended for farmers interested in raising Nigerian native chickens. 相似文献
13.
Rizwan Rasheed Muhammad Iqbal Iqbal Hussain Fahad Shafiq Atiqa Yousaf 《The Journal of Horticultural Science and Biotechnology》2018,93(4):385-391
Glycine betaine (GB) is an important organic compound mediating plant responses to environmental stresses. However, despite ample research on this biomolecule, the potential of GB in mitigating the effects of waterlogging in plants has not been established. Therefore, we studied the influence of GB on growth and physiology of tomato plants under waterlogged conditions. Waterlogging reduced plant growth, degraded chlorophyll, and increased concentration of malondialdehyde and hydrogen peroxide that deteriorated membrane integrity. Waterlogging increased catalase and peroxidase activities. Waterlogging increased the concentration of Na and reduced concentrations of K. Reductions in root Ca were also recorded. GB enhanced growth, concentration of chlorophyll and activities of superoxide dismutase, catalase, and peroxidase that in turn protected the plants from oxidative damage. GB decreased Na while increasing leaf and root K and stem and fruit Ca under waterlogging. Waterlogging reduced fruit quality. There was decrease in protein, fat, and total dissolved solids and an increase in fruit moisture, P, and Na in plants under waterlogging. GB enhanced fruit quality largely by improving fruit protein, ash, fat, TDS, and Ca, while it decreased fruit Na. The results of this study suggest the use of GB for commercial production of tomato where waterlogging is likely. 相似文献
14.
Hasan Riaz Muhammad Rizwan Yousuf Aixin Liang Guo Hua Hua Liguo Yang 《Animal Science Journal》2019,90(4):473-480
This study was aimed to address melatonin receptor expression, mRNA level of hypothalamus and hypophysis hormone receptors (GnRHR, FSHR, and LHR), steroidogenesis, cell cycle, apoptosis, and their regulatory factors after addition of melatonin for 24 hr in cultured buffalo granulosa cells (GCs). The results revealed that direct addition of different concentrations of melatonin (100 pM, 1 nM, and 100 nM) resulted in significant upregulation (p < 0.05) of mRNA level of melatonin receptor 1a (MT1) without affecting melatonin receptor 1b (MT2). Melatonin treatment significantly downregulated (p < 0.05) mRNA level of FSH and GnRH receptors, whereas 100 nM dose of melatonin significantly increased mRNA level of LH receptor. Treatment with 100 nM of melatonin significantly decreased the basal progesterone production with significant decrease (p < 0.05) in mRNA levels of StAR and p450ssc, and lower mRNA level of genes (Insig1, Lipe, and Scrab1) that affect cholesterol availability. Melatonin supplementation suppressed apoptosis (100 nM, p < 0.05) and enhanced G2/M phase (1 nM, 100 nM, p < 0.05) of cell cycle progression which was further corroborated by decrease in protein expression of caspase‐3, p21, and p27 and increase in bcl2. Our results demonstrate that melatonin regulates gonadotrophin receptors and ovarian steroidogenesis through MT1. Furthermore, the notion of its incorporation in apoptosis and proliferation of buffalo GCs extends its role in buffalo ovaries. 相似文献
15.
Awais Rasheed Tariq Mahmood Alvina Gul Kazi Abdul Ghafoor Abdul Mujeeb-Kazi 《Journal of Crop Science and Biotechnology》2012,15(1):1-7
The objective of this study was to identify allelic variations at Glu-1 loci of wheat (Triticum aestivum L.) advanced lines derived from hybridization of bread wheat and synthetic hexaploid wheats (2n = 6x = 42; AABBDD). Locally
adapted wheat genotypes were crossed with synthetic hexaploid wheats. From the 134 different cross combinations made, 202
F8 advanced lines were selected and their HMW-GS composition was studied using SDS-PAGE. In total, 24 allelic variants and 68
HMW-GS combinations were observed at Glu-A1, Glu-B1, and Glu-D1 loci. In bread wheat, the Glu-D1 locus is usually characterized by subunits 1Dx2+1Dy12 and 1Dx5+1Dy10 with the latter having a stronger effect on bread-making
quality. The subunit 1Dx5+1Dy10 was predominantly observed in these advanced lines. The inferior subunit 1Dx2+1Dy12, predominant
in adapted wheat germplasm showed a comparative low frequency in the derived advanced breeding lines. Its successful replacement
is due to the other better allelic variants at the Glu-D1 locus inherited in these synthetic hexaploid wheats from Aegilops tauschii (2n = 2x = 14; DD). 相似文献
16.
Baloch Abdul Rasheed Franěk Roman Saito Taiju Pšenička Martin 《Fish physiology and biochemistry》2021,47(3):777-784
Fish Physiology and Biochemistry - Dead end (dnd) is a germ plasm-specific maternal RNA discovered in zebrafish and then in other vertebrates. Dnd protein is essential for migration and motility of... 相似文献
17.
Application of natural plant extracts improves the tolerance against combined terminal heat and drought stresses in bread wheat 
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下载免费PDF全文 M. Farooq M. Rizwan A. Nawaz A. Rehman R. Ahmad 《Journal of Agronomy and Crop Science》2017,203(6):528-538
Drought and heat are among the main abiotic stresses causing severe damage to the cereal productivity when occur at reproductive stages. In this study, ten wheat cultivars were screened for combined heat and drought tolerance imposed at booting, heading, anthesis and post‐anthesis stages, and role of the foliage applied plant extracts was evaluated in improving the performance of differentially responding wheat cultivars under terminal heat and drought stresses. During both years, wheat crop was raised under ambient temperature and 70% water holding capacity (WHC) till leaf boot stage. The plant extracts (3% each) of sorghum, brassica, sunflower and moringa were foliage applied at booting, anthesis and post‐anthesis stage; and after one week of application of these plant extracts, combined heat and drought was imposed at each respective stage. Heat and drought stresses were imposed at each respective stage by placing pots in glass canopies with temperature of 4 ± 2°C above than the ambient temperature in combination with drought stress (35% WHC) until maturity. Combination of drought and heat stresses significantly reduced the performance of tested wheat cultivars; however, stress at the booting and heading stages was more damaging than the anthesis and post‐anthesis stages. Cultivars Mairaj‐2008 and Chakwal‐50 remained green with extended duration for grain filling, resulting in the maintenance of number of grains per spike and 100‐grain weight under stress conditions and thus had better grain yield and water‐use efficiency. However, in cultivars Fsd‐2008, and Shafaq‐2006, the combined imposition of drought and heat accelerated the grain filling rate with decrease in grain filling duration, grain weight and grain yield. Foliar application of all the plant extracts improved the wheat performance under terminal heat and drought stress; however, brassica extract was the most effective. This improvement in grain yield, water‐use efficiency and transpiration efficiency due to foliage applied plant extracts, under terminal heat and drought stress, was owing to better stay‐green character and accumulation of more soluble phenolics, which imparted stress tolerance as indicated by relatively stable grain weight and grain number. In crux, growing of stay‐green wheat cultivars with better grain filling and foliage application of plant extracts may help improving the performance of bread wheat under combined heat and drought stresses. 相似文献
18.
Y Pan Y Cui S Yu Q Zhang J Fan B Abdul Rasheed K Yang 《Reproduction in domestic animals》2014,49(6):970-976
Growth factors play critical role in cell proliferation, regulate tissue differentiation and modulate organogenesis. Several growth factors have been identified in the testes of various mammalian species in last few years. In present investigation, the objective was to determine the expression of epidermal growth factor (EGF) and the epidermal growth factor receptor (EGFR) in yak testicular tissue by relative quantitative real time polymerase chain reaction (RT‐PCR), Western blot (WB) and immunohistochemistry (IHC) from mRNA and protein levels. The testicular tissues were collected from male yak at 6 and 24 months old. Results of RT‐PCR and WB showed that the expression quantity of EGF and EGFR at 24 months of age was higher than at 6 months, and the increase rate of EGFR on mRNA and protein levels was higher than the increase rate EGF during post‐natal testes development. Positive staining for EGF and EGFR was very low and mainly localized to Leydig cells testes at 6 months of age with immunohistochemistry, and seminiferous tubules were not observed. At 24 month of age, both the EGF and EGFR could be detected in Leydig cells, peritubular myoid cells, sertoli cells and germ cells of the yak testes. However, EGF and EGFR were localized to preferential adluminal compartment and basal compartment in the seminiferous tubules, respectively. In conclusion, the findings in present studies suggest that EGF and EGFR as important paracrine and/or autocrine regulators in yak testes development and spermatogenesis. 相似文献
19.
Heaphy CM de Wilde RF Jiao Y Klein AP Edil BH Shi C Bettegowda C Rodriguez FJ Eberhart CG Hebbar S Offerhaus GJ McLendon R Rasheed BA He Y Yan H Bigner DD Oba-Shinjo SM Marie SK Riggins GJ Kinzler KW Vogelstein B Hruban RH Maitra A Papadopoulos N Meeker AK 《Science (New York, N.Y.)》2011,333(6041):425
The proteins encoded by ATRX and DAXX participate in chromatin remodeling at telomeres and other genomic sites. Because inactivating mutations of these genes are common in human pancreatic neuroendocrine tumors (PanNETs), we examined the telomere status of these tumors. We found that 61% of PanNETs displayed abnormal telomeres that are characteristic of a telomerase-independent telomere maintenance mechanism termed ALT (alternative lengthening of telomeres). All of the PanNETs exhibiting these abnormal telomeres had ATRX or DAXX mutations or loss of nuclear ATRX or DAXX protein. ATRX mutations also correlate with abnormal telomeres in tumors of the central nervous system. These data suggest that an alternative telomere maintenance function may operate in human tumors with alterations in the ATRX or DAXX genes. 相似文献
20.
The removal of nitrate reductase phosphorylation enhances tolerance to ammonium nitrogen deficiency in rice 下载免费PDF全文
下载免费PDF全文 HAN Rui-cai XU Zhi-rong LI Chen-yan Adnan Rasheed PAN Xiao-hua SHI Qing-hua WU Zi-ming 《农业科学学报》2022,21(3):631-643
Nitrate reductase (NR) is a key enzyme for nitrogen assimilation in plants, and its activity is regulated by posttranslational phosphorylation. To investigate the effects of dephosphorylation of the NIA1 protein on the growth and the physiological and biochemical characteristics of rice under different forms of nitrogen supplies, the phenotypes, nitrogen metabolism and reactive oxygen metabolism were measured in NIA1 phosphorylation site-directed mutant lines (S532D and S532A), an OsNia1 over-expression line (OE) and Kitaake (wild type, WT). Compared with WT and OE, S532D and S532A have stronger nitrogen assimilation capacities. When ammonium nitrate served as the nitrogen source, the plant heights, dry weights of shoots and chlorophyll (Chl) contents of S532D and S532A were lower than those of the WT and OE, whereas hydrogen peroxide (H2O2), malondialdehyde (MDA) and nitrite contents were higher. When potassium nitrate served as the nitrogen source, the plant heights, dry weights of shoots and Chl contents of S532D and S532A were higher than those of the WT and OE, there were no significant differences in the contents of H2O2 and MDA in the leaves of the test materials, and the difference in nitrite contents among different lines decreased. When ammonium sulfate served as the nitrogen source, there were no significant differences in the physiological indexes of the test materials, except NR activity. Compared with ammonium nitrate and ammonium sulfate, the content of NH4+-N in the leaves of each plant was lower when potassium nitrate was used as the nitrogen source. The qPCR results showed that OsGS and OsNGS1 were negatively regulated by downstream metabolites, and OsNrt2.2 was induced by nitrate. In summary, when ammonium nitrate served as the nitrogen source, the weak growth of NIA1 phosphorylation site-directed mutant lines was due to the toxicity caused by the excessive accumulation of nitrite. When potassium nitrate served as the nitrogen source, the assimilation rates of nitrate, nitrite and ammonium salt were accelerated in NIA1 phosphorylation site-directed mutant lines, which could provide more nitrogen nutrition and improve the tolerance of rice to ammonium nitrogen deficiency. These results could provide a possible method to improve the efficiency of nitrogen utilization in rice under low-nitrogen conditions. 相似文献