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11.
Zahra Ranjbar Mehran Torki Amir Ali Karimi Torshizi 《Journal of animal physiology and animal nutrition》2019,103(5):1418-1426
In ovo injection (IOI) of Naringin (N), flavanone was examined on post‐hatch blood biochemical parameters, antioxidant status and bone characteristics. Fertile eggs (n = 700) were distributed in seven groups with 100 eggs. On 14th and 17.5th days of incubation, four groups were injected using 15 or 30 mg active ingredient levels of naringin/0.5 ml saline/egg, low and high level, into amnion sac. Controls include sham (injected normal saline, 0.5 ml/egg on day 14 and 17.5th) and un‐injected group. IOI of high naringin and saline on 14th day of incubation resulted in lower hatchability and then higher mortality in last week of embryonic life. On day hatch, high levels of injected groups more body weight compared to the control. Chick length was increased at high levels of naringin on day 17.5th compared to control and saline injected. Quality traits of bones were improved in naringin‐injected groups compared to control. IOI of naringin influenced thyroid hormones on 14th day of incubation. Naringin groups influenced the Alkaline phosphatase (ALP), Calcium (Ca), superoxide dismutase (SOD), blood biochemical and lipids. Totally, amniotic IOI of naringin in last days of developing embryo may be useful for hatched chick, development of leg long bone or effect on biochemical metabolites by levels of flavanone that it needs more research. 相似文献
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Rouhollah Karimi Abdolhossein Rezaei Nejad Shahrokh Khanizadeh 《The Journal of Horticultural Science and Biotechnology》2016,91(4):386-395
The effects of exogenous application of abscisic acid (ABA) on anti-oxidant enzyme activities and photosynthetic capacity in ‘Sultana’ grapevine (Vitis vinifera L.) were investigated under cold stress. When vines had an average of 15 leaves, 0 (control), 50, 100, or 200 µM ABA was sprayed to run-off on all leaves of each plant. Twenty-four hours after foliar spraying with ABA, half (n = 5) of the water-only control vines and half (n = 5) of each group of ABA-treated plants were subjected to 4°C for 12 h, followed by a recovery period of 3 d under greenhouse conditions (25°/18°C day/night). The remaining plants in each treatment group were kept at 24°C. Cold stress increased H2O2 and malondialdehyde (MDA) concentrations in vine leaves, whereas all foliar ABA treatments significantly reduced their levels. Chilled plants showed marked increases in their total soluble protein contents in response to each ABA treatment. ABA significantly increased the activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase in cold-stressed grapevine leaves. In contrast, cold stress markedly decreased the rates of leaf photosynthesis (A) and evaporation (E), stomatal conductance (gs), and chlorophyll concentrations in leaves, but increased intercellular CO2 concentrations (Ci) in leaves. Treatment with all concentrations of ABA resulted in lower leaf A, E, and gs values, but higher Ci values at 24°C. However, following cold stress, ABA-treated vines showed higher leaf A, E, and gs values, but lower Ci values compared to control vines without ABA treatment. The application of 50–200 µM ABA allowed chilled vines to recover more quickly when re-exposed to normal temperatures, enabling the vines to resume their photosynthetic capacity more efficiently following cold stress. These results showed that, by stimulating anti-oxidant enzyme systems and alleviating cold-induced stomatal limitations, ABA reduced the inhibitory effect of cold stress on the rate of CO2 fixation in ‘Sultana’ grapevine plants. 相似文献
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Leila Behbood Soroush Karimi Esmaeil Mirzaei Ghobad Mohammadi Mahsa Azami Elham Arkan 《Fibers and Polymers》2018,19(7):1454-1462
The mucoadhesive Chitosan (CS) nanofibers as a drug delivery system were developed. Chitosan was modified via the immobilization of thiol groups from L-cysteine as a mucoadhesive reagent. The mucoadhesive properties of the chitosan nanofibers were evaluated by tensiometer set and via tensile studies. Drug and mucoadhesive agent loading lead to decrease diameters and increased porous of nanofibers. The release of Tetracycline (Tet) and Triamcinolone (Tri) were increased with increasing immersion time and it became constant at long immersion times. Mucoadhesion studies were done at pH 2–7 and in pH 6 maximum mucoadhesive properties observed. Release studies demonstrated a sustained release of both drug continued up to 48 hours. Microbial studies were performed on the nanofibers. The drug delivery system represented a novel tool for improve the therapeutic efficacy of various drugs that are poorly absorbed from the gastrointestinal tract. Also it is an efficient system for treatment of oral ulceration. 相似文献
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Ali Asghar Karimi Marjan Ajami Yasin Asadi Nahid Aboutaleb Fazel Gorjipour Roya Malekloo Hamidreza Pazoki-Toroudi 《Iranian Biomedical Journal》2015,19(2):111-116
Background:
Skin flap grafting is a popular approach for reconstruction of critical skin and underlying soft tissue injuries. In a previous study, we demonstrated the beneficial effects of two 5α-reductase inhibitors, azelaic acid and finasteride, on tissue survival in a rat model of skin flap grafting. In the current study, we investigated the involvement of nitric oxide and inducible nitric oxide synthase (iNOS) in graft survival mediated by these agents.Methods:
A number of 42 male rats were randomly allocated into six groups: 1, normal saline topical application; 2, azelaic acid (100 mg/flap); 3, finasteride (1 mg/flap); 4, injection of L-NG-nitroarginine methyl ester (L-NAME) (i.p., 20 mg/kg); 5, L-NAME (20 mg/kg, i.p.) + azelaic acid (100 mg/flap, topical); 6, L-NAME (20 mg/kg, i.p.) + finasteride (1 mg/flap, topical). Tissue survival, level of nitric oxide, and iNOS expression in groups were measured.Results:
Our data revealed that azelaic acid and finasteride significantly increased the expression of iNOS protein and nitric oxide (NO) levels in graft tissue (P < 0.05). These increases in iNOS expression and NO level were associated with higher survival of the graft tissue.Conclusion:
It appears that alterations of the NO metabolism are implicated in the azelaic acid- and finasteride-mediated survival of the skin flaps.Key Words: Finasteride, Azelaic acid, Surgical flaps, Nitric oxide, Nitric oxide synthase Type II 相似文献16.
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Mohammadzadeh Sedigheh Milla Sylvain Ahmadifar Ehsan Karimi Masoumeh Dawood Mahmoud A. O. 《Fish physiology and biochemistry》2021,47(4):951-960
The use of recombinant gonadotropin-releasing hormone (rGnRH) has very rarely been tested in fish to promote spawning. This study evaluated the impact of recombinant chicken gonadotropin-releasing hormone (rcGnRH) with metoclopramide on the release of sex steroids and final maturation induction in goldfish (Carassius auratus) broodstock. For this purpose, goldfish broodstock was divided into four groups and treated with 0.9% NaCl with 20 mg/kg metoclopramide (Met) (C); 10 μg/kg body weight (BW) rcGnRH with 20 mg/kg metoclopramide (rcGn10); 15 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn15); and 20 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn20). The capability of the rcGnRH for eliciting biological response was tested in vivo by evaluating the changes of 17β estradiol (E2), testosterone (T), and 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) and the induced spawning. Blood samples were obtained at 0 h, 12 h, and 24 h after injection. The rcGn10, rcGn15, and rcGn20 treatments induced lower E2 concentration, especially 24 h post-injection. T levels were significantly higher in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection than at 0 h and then decreased at 24 h post-injection. Furthermore, the rcGnRH tested significantly enhanced DHP secretion in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection before a decline at 24 h post-injection. No significant difference between the sampling times was found in the C treatment for the 3 sex steroids tested. The results also displayed that rcGnRH at 10–20 µg/kg of body weight can trigger spawning with the highest speed and efficiency of spawning at 20 µg/kg. The obtained results represent a possible strategy for enhancing the artificial reproduction and ovulation of broodstock fish by rGnRH and further support the use of recombinant hormones to promote reproduction in aquaculture.
相似文献19.
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Issa Yar Mohammad Karimi Mohammed Abdul Mohsen Ali Salem Abdul Jaleel Cheruth Fayas Thayale Purayil Sreeramanan Subramaniam 《Journal of plant nutrition》2018,41(7):888-902
In the arid regions, turfgrass cover is an integral part of landscape to protect the soil from erosion, enhance the aesthetic value, and improve the microclimate. The salinity and the scarcity of fresh water of the arid region are the major challenging factors in turfgrass production. Therefore, the need for salt tolerant turfgrass with functional quality is necessary to improve the turf performance. The detrimental effects of salinity include growth suppression, and lowered osmotic potential ultimately leading to firing of the leaf blades. In this context, the study was undertaken to determine the relative salt tolerance and growth response of turfgrass genotypes in order to recommend turfgrass cultivars that can tolerate high salinity irrigation and maintain excellent visual and functional qualities under United Arab Emirates (UAE) condition. The paspalum cultivars maintained the highest succulence percentage compared to the bermudagrass cultivars under enhanced salinity levels. The shoots count, fresh weight (FW), and dry weight (DW) were found highest in paspalum types. The chlorophyll a, chlorophyll b and the total chlorophyll content was found higher in bermuda grass types under high salinity levels. The bermudagrass cultivars showed significantly higher carotenoids, anthocyanins and proline compared to the paspalum types under salt stress condition. In the case of princess 77 and Yukon, an inherently high amount of proline was recorded which confirmed an increase up to 10,000 ppm and drastically declined beyond this concentration. Sea Dwarf paspalum and Sea Isle 2000 maintained uniformity in the proline level at all levels of salinity without significant variation. These findings point to the fact that both paspalum and bermuda types exhibited varied responses to different physiological and biochemical parameters under the saline conditions. Paspalum types have an edge over the bermudagrass in terms of shoot density, which is a potential factor in determining the high-quality turfs. Bermudagrass types can be applied in lower salinity conditions based on the responses as evidenced from the present results. 相似文献