全文获取类型
收费全文 | 1618篇 |
免费 | 130篇 |
专业分类
林业 | 81篇 |
农学 | 33篇 |
基础科学 | 7篇 |
274篇 | |
综合类 | 172篇 |
农作物 | 50篇 |
水产渔业 | 90篇 |
畜牧兽医 | 885篇 |
园艺 | 55篇 |
植物保护 | 101篇 |
出版年
2024年 | 5篇 |
2023年 | 28篇 |
2022年 | 31篇 |
2021年 | 70篇 |
2020年 | 58篇 |
2019年 | 68篇 |
2018年 | 61篇 |
2017年 | 61篇 |
2016年 | 44篇 |
2015年 | 46篇 |
2014年 | 66篇 |
2013年 | 91篇 |
2012年 | 134篇 |
2011年 | 166篇 |
2010年 | 76篇 |
2009年 | 65篇 |
2008年 | 116篇 |
2007年 | 84篇 |
2006年 | 97篇 |
2005年 | 62篇 |
2004年 | 62篇 |
2003年 | 56篇 |
2002年 | 35篇 |
2001年 | 17篇 |
2000年 | 15篇 |
1999年 | 10篇 |
1998年 | 10篇 |
1997年 | 10篇 |
1996年 | 3篇 |
1995年 | 13篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 7篇 |
1991年 | 12篇 |
1990年 | 9篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 4篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1977年 | 3篇 |
1973年 | 4篇 |
1972年 | 2篇 |
1970年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有1748条查询结果,搜索用时 15 毫秒
41.
42.
Parker S Campbell J McIntosh K Gajadhar A 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(9):732-734
The diagnostic test for Tritrichomonas foetus in bulls is microscopic examination of cultured preputial samples. Trichomonads other than T. foetus can be present in a preputial sample. Both a staining technique and a polymerase chain reaction assay were useful in differentiating between T. foetus and another trichomonad observed in samples from virgin bulls. 相似文献
43.
Full pedigree quantitative trait locus analysis in commercial pigs using variance components 总被引:1,自引:0,他引:1
de Koning DJ Pong-Wong R Varona L Evans GJ Giuffra E Sanchez A Plastow G Noguera JL Andersson L Haley CS 《Journal of animal science》2003,81(9):2155-2163
In commercial livestock populations, QTL detection methods often use existing half-sib family structures and ignore additional relationships within and between families. We reanalyzed the data from a large QTL confirmation experiment with 10 pig lines and 10 chromosome regions using identity-by-descent (IBD) scores and variance component analyses. The IBD scores were obtained using a Monte Carlo Markov Chain method, as implemented in the LOKI software, and were used to model a putative QTL in a mixed animal model. The analyses revealed 61 QTL at a nominal 5% level (out of 650 tests). Twenty-seven QTL mapped to areas where QTL have been reported, and eight of these exceeded the threshold to claim confirmed linkage (P < 0.01). Forty-two of the putative QTL were detected previously using half-sib analyses, whereas 46 QTL previously identified by half-sib analyses could not be confirmed using the variance component approach. Some of the differences could be traced back to the underlying assumptions between the two methods. Using a deterministic approach to estimate IBD scores on a subset of the data gave very similar results to LOKI. We have demonstrated the feasibility of applying variance component QTL analysis to a large amount of data, equivalent to a genome scan. In many situations, the deterministic IBD approach offers a fast alternative to LOKI. 相似文献
44.
Kahn S Geale DW Kitching PR Bouffard A Allard DG Duncan JR 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(5):349-354
Vaccination of susceptible animals against foot-and-mouth disease (FMD) is a well established strategy for helping to combat the disease. Traditionally, FMD vaccine has been used to control a disease incursion in countries where the disease has been endemic rather than in countries considered free of the disease. In 2001, the use of vaccine was considered but not implemented in the United Kingdom (1), whereas vaccine was used to help to control FMD in The Netherlands (2,3). Canadian contingency plans provide for the use of vaccine; Canada is a member of the North American Foot-and-Mouth Disease Vaccine Bank, which could supply vaccine if needed. This article explains why Canada might use FMD vaccine to combat an outbreak and the factors that are relevant to the disposal of vaccinated animals and their products. It concludes that vaccination is an important mechanism in Canada's preparedness for an outbreak of FMD and that products from vaccinated animals are safe for human consumption. 相似文献
45.
McTavish S 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(6):463-465
A 9-year-old mixed-breed dog was evaluated for chronic intermittent vomiting, hematemesis, and melena lasting several months. Biopsy specimens obtained during exploratory laparotomy revealed eosinophilic gastroenteritis. Treatment included drug therapy to reduce gastrointestinal inflammation and dietary management to limit antigenic exposure. 相似文献
46.
Sarah Cox M.A. Mayo A. Teifion Jones 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(4):353-364
Analysis was made of dsRNA in 37 cultivars and species of Ribes, that were healthy, naturally affected with the virus-like diseases, blackcurrant yellows, blackcurrant infectious variegation, gooseberry veinbanding or blackcurrant reversion, or graft-inoculated with scions from such diseased plants. Various dsRNA species, differing in size (from ca. 2 to 11kbp), number and staining intensity in gels, were detected in some or all assays of all plants, including those held as virus-tested stock. In different plant tissues from individual plants, the dsRNA species were usually similar in size and number but, in some sources, the dsRNA profile from flowers and/or bark differed greatly from the profiles of dsRNA obtained from leaves. No dsRNA species were associated consistently with any of these diseases. A 499kbp cDNA probe was obtained that in Northern blot analysis was specific to a ca. 5kbp dsRNA species present in the blackcurrant cv. Baldwin. It also detected a similarly sized dsRNA species in plants of many other blackcurrant cultivars, but it did not react with a similarly sized dsRNA species in redcurrant and gooseberry tissues. The 156 amino acid sequence encoded by the cDNA was very similar to sequences in the RNA-directed RNA polymerases of virus species in the family Totiviridae, especially Saccharomyces cerevisiae viruses L-1 and L-A. The significance of these findings and the possible origin of these dsRNA species are discussed. 相似文献
47.
48.
49.
C Kopp A Sukura E Tuunainen I Gustavsson M Parvinen M Andersson 《Reproduction in domestic animals》2007,42(2):208-213
The development and use of modern techniques, such as intracytoplasmic sperm injection (ICSI), gene knockout and sperm fluorescence in situ hybridization with chromosome- specific probes, have significantly increased our knowledge about sperm defects. We describe a new oligoasthenoteratozoospermic defect in a bull. Because of its morphological characteristics the defect was named the multinuclear-multiflagellar sperm defect. All spermatozoa in the ejaculate were abnormal. Many of the spermatozoa had multiple nuclei and multiple sperm tails. All spermatozoa lacked an acrosome, and only seldom did spermatozoa have a mitochondrial helix in the midpiece area. Meiosis and spermiogenesis were severely affected in this otherwise phenotypically normal bull. The sperm defects resembled the phenotype of a targeted gene knockout Hrb(-/-) (HIV-1 Rev-binding/interacting protein) mutant mouse strain, which is expressed as sterility in males, while females remain fertile. Since the father of this bull has been extensively used in at least three countries the defective gene has possibly become widespread in the red and white breeds (Ayrshire, Swedish Red and White, Norwegian Red) in the Nordic countries. However, it is not proved that the father of this bull is a carrier of this defect. 相似文献
50.
This article offers information regarding selected veterinary information resources, along with basic search strategies for locating clinical evidence within these resources. No one database provides adequate indexing and abstracting to all literature relevant to the veterinary clinical question. An understanding of a database's syntax and field structure is necessary to formulate a functional search strategy and evaluate the outcome of search results. Flexibility when identifying, selecting, and combining search terms is also required to avoid overlimiting a search. 相似文献