Hypertrophic osteopathy associated with hepatocellular carcinoma in a dog     

Victoria D. Randall  Carlos Souza  Daniel Vanderhart  Sarah Boston 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(7):741-744

A 9-year-old spayed female dog diagnosed with hepatocellular carcinoma and hypertrophic osteopathy was negative for additional lesions on computed tomography of the thorax and abdomen. Resection of the affected liver lobe resulted in resolution of clinical signs. This is the first case of hypertrophic osteopathy secondary to hepatocellular carcinoma.  相似文献   

Effects of interferon-γ knockdown on vaccine-induced immunity against Marek’s disease in chickens     

Kamran Haq  Sarah K. Wootton  Neda Barjesteh  Serguei Golovan  Andrew Bendall  Shayan Sharif 《Canadian journal of veterinary research》2015,79(1):1-7

Interferon (IFN)-γ has been shown to be associated with immunity to Marek’s disease virus (MDV). The overall objective of this study was to investigate the causal relationship between IFN-γ and vaccine-conferred immunity against MDV in chickens. To this end, 3 small interfering RNAs (siRNAs) targeting chicken IFN-γ, which had previously been shown to reduce IFN-γ expression in vitro, and a control siRNA were selected to generate recombinant avian adeno-associated virus (rAAAV) expressing short-hairpin small interfering RNAs (shRNAs). An MDV challenge trial was then conducted: chickens were vaccinated with herpesvirus of turkey (HVT), administered the rAAAV expressing shRNA, and then challenged with MDV. Tumors were observed in 4 out of 10 birds that were vaccinated with HVT and challenged but did not receive any rAAAV, 5 out of 9 birds that were administered the rAAAV containing IFN-γ shRNA, and 2 out of 10 birds that were administered a control enhanced green fluorescent protein siRNA. There was no significant difference in MDV genome load in the feather follicle epithelium of the birds that were cotreated with the vaccine and the rAAAV compared with the vaccinated MDV-infected birds. These results suggest that AAAV-based vectors can be used for the delivery of shRNA into chicken cells. However, administration of the rAAAV expressing shRNA targeting chicken IFN-γ did not seem to fully abrogate vaccine-induced protection.  相似文献   

Red blood cell storage lesion          下载免费PDF全文

Rafael Obrador LVS  DACVECC  Sarah Musulin DVM  DACVECC  Bernie Hansen DVM  MS  DACVECC  DACVIM 《Journal of Veterinary Emergency and Critical Care》2015,25(2):187-199

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Formation of amyloid-like fibrils by ovalbumin and related proteins under conditions relevant to food processing     

Pearce FG  Mackintosh SH  Gerrard JA 《Journal of agricultural and food chemistry》2007,55(2):318-322

Protein aggregation is important in food processing, and this work investigated the aggregation of food proteins as a source of amyloid fibrils for use in bionanotechnology. Both purified and crude mixtures of albumin proteins were denatured by heat, which caused aggregation to occur. Protein denaturation was measured by using circular dichroism spectrometry and by following thioflavin T fluorescence, which is widely used as a diagnostic test for amyloid formation. There was a good correlation between the increase in thioflavin T fluorescence and loss of helical structure as the temperature was increased. Formation of thioflavin T fluorescence was dependent on temperature, but less dependent on salt and protein concentration. X-ray fiber diffraction patterns of denatured bovine serum albumin suggested that the protein had a similar cross-beta structure to that of amyloid fibrils. These results are consistent with the aggregates seen during food processing, being amyloid-like in nature.  相似文献   

Physiological and metabolic properties of a digestion-resistant maltodextrin, classified as type 3 retrograded resistant starch     

Brouns F  Arrigoni E  Langkilde AM  Verkooijen I  Fässler C  Andersson H  Kettlitz B  van Nieuwenhoven M  Philipsson H  Amado R 《Journal of agricultural and food chemistry》2007,55(4):1574-1581

There is a growing interest in highly fermentable dietary fibers having the potential to reduce risks of disease through the production of short-chain fatty acids (SCFA). Recently a digestion-resistant retrograded maltodextrin (RRM), classified as type 3 resistant starch was developed. Systematic work to determine its molecular and physiological properties was carried out to determine (1) the fraction resistant to digestion in vitro and in vivo, (2) its postconsumption effect on blood glucose in healthy volunteers, and (3) its in vitro fermentation pattern, at different ages, by use of pooled fresh human fecal inoculum. RESULTS: The digestion resistant fraction obtained in vivo from ileostomy patients (59.4%) is similar to that obtained by the AOAC method for measuring retrograded resistant starch (59.7%). The relative glycemic response after consumption of 50 g of RRM was 58.5% compared to glucose set as 100%. When exposed to colonic microbiota, in vitro obtained indigestible fractions behave similarly to those obtained in vivo in ileostomy patients. Fermentation of RRM and production of butyric acid is negligible during the first months of life but develops subsequently during weaning. In adults, RRM fermentation results in a high yield of SCFA, with butyrate representing 21-31 mol % of total SCFA. The high yield of SCFA during colonic fermentation, observed from weaning age on, as well as the potential to help reduce glycemic load may be of benefit to a number of health-related functions in the host. Further study on clear clinical end points is warranted.  相似文献   

Studies on N-terminal glycation of peptides in hypoallergenic infant formulas: quantification of alpha-N-(2-furoylmethyl) amino acids     

Penndorf I  Biedermann D  Maurer SV  Henle T 《Journal of agricultural and food chemistry》2007,55(3):723-727

To obtain information about the extent of the early Maillard reaction between the N-termini of peptides and lactose, alpha-N-(2-furoylmethyl) amino acids (FMAAs) were quantified together with epsilon-N-(2-furoylmethyl)lysine (furosine) in acid hydrolyzates of hypoallergenic infant formulas, conventional infant formulas, and human milk samples using RP-HPLC with UV-detection. FMAAs are formed during acid hydrolysis of peptide-bound N-terminal Amadori products (APs), and furosine is formed from the Amadori products of peptide-bound lysine. Unambiguous identification was achieved by means of LC/MS and UV-spectroscopy using independently prepared reference material. The extent of acid-induced conversion of APs to FMAAs was studied by RP-HPLC with chemiluminescent nitrogen detection (CLND). Depending on the corresponding alpha-N-lactulosyl amino acid, between 6.0% and 18.1% of FMAAs were formed during hydrolysis for 23 h at 110 degrees C in 8 N HCl. From epsilon-N-lactulosyllysine, 50% furosine is formed under these conditions. Whereas furosine was detectable in all assayed samples, five different FMAAs, alpha-FM-Lys, alpha-FM-Ala, alpha-FM-Val, alpha-FM-Ile, and alpha-FM-Leu, were exclusively detected in acid hydrolyzates of hypoallergenic infant formulas in amounts ranging from 35 to 396 mumol/100 g protein. Taking the conversion factors into account, modification of N-terminal amino acids in peptides by reducing carbohydrates was between 0.3% and 8.4%. This has to be considered within the discussion concerning the nutritional quality of peptide-containing foods.  相似文献   

Olfactory responses of Argentine stem weevil to herbivory and endophyte-colonisation in perennial ryegrass     

Hennessy  Louise M.  Popay  Alison J.  Glare  Travis R.  Finch  Sarah C.  Cave  Vanessa M.  Rost&#;s  Michael 《Journal of pest science》2022,95(1):263-277

Journal of Pest Science - Argentine stem weevil adults (ASW, Listronotus bonariensis) feed on the leaves of agricultural grasses and their larvae mine the pseudostem, causing extensive damage that...  相似文献   

Spatial and temporal genetic variation of Drosophila suzukii in Germany     

Petermann  Sarah  Otto  Sabine  Eichner  Gerrit  Schetelig  Marc F. 《Journal of pest science》2021,94(4):1291-1305

Native to Southeast Asia, the spotted wing drosophila (SWD), Drosophila suzukii Matsumura, rapidly invaded America and Europe in the past 20 years. As a crop pest of soft-skinned fruits with a wide range of host plants, it threatens the fruit industry worldwide, causing enormous economic losses. To control this invasive pest species, an understanding of its population dynamics and structure is necessary. Here, we report the population genetics and development of SWD in Germany from 2017–19 using microsatellite markers over 11 different sample sites. It is the first study that examines SWD’s genetic changes over 3 years compared to multiple international SWD laboratory strains. Results show that SWD populations in Germany are highly homogenous without differences between populations or years, which indicates that populations are well adapted, migrate freely, and multiple invasions from outside Germany either did not take place or are negligible. Such high genetic variability and migration between populations could allow for a fast establishment of the pest species. This is especially problematic with regard to the ongoing spread of this invasive species and could bear a potential for developing pesticide resistance, which could increase the impact of the SWD further in the future.

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Total Cystectomy for Treatment of Transitional Cell Carcinoma of the Urethra and Bladder Trigone in a Dog     

Sarah Boston DVM  DVSc  Diplomate ACVS  Ameet Singh DVM  DVSc  Diplomate ACVS 《Veterinary surgery : VS》2014,43(3):294-300

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A Systematic Review of the Literature Describing the Efficacy of Surgical Treatments for Canine Hip Dysplasia (1948–2012)     

Mary Sarah Bergh DVM  MS  Diplomate ACVS & ACVSMR  Steven C. Budsberg DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》2014,43(5):501-506

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