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11.
J K Collins C Bruns T L Vermedahl A L Schiebel M T Jessen P C Schultheiss G M Anderson R P Dinsmore R J Callan J C DeMartini 《Journal of veterinary diagnostic investigation》2000,12(5):406-411
Using a polymerase chain reaction (PCR) test for sequences of ovine herpesvirus 2 (OHV2), this virus was shown to be significantly associated with sheep-associated malignant catarrhal fever (SA-MCF) in terminal cases of disease in 34 cattle and 53 bison. Ovine herpesvirus 2 was not detected in cattle (38) and bison (10) that succumbed to other diseases. Other persistent herpesviruses, retroviruses, and pestivirus, some of which have been previously isolated from cases of SA-MCF, were not associated with the disease. These included bovine herpesvirus 4 (BHV4), bovine lymphotrophic herpesvirus (BLHV), bovine syncytial virus (BSV, also known as bovine spumavirus), bovine immunodeficiency virus (BIV), and bovine viral diarrhea virus (BVDV). A PCR survey for OHV2 in DNA from individual cow's peripheral blood lymphocytes in 4 dairies showed that the 1 dairy that was in close contact to sheep had a prevalence of OHV2 of 21.3%, whereas the 3 other dairies had no OHV2. Prevalence of the other herpesviruses and retroviruses in the dairy cows was variable, ranging from 2% to 51% for BHV4, 52% to 78.7% for BLHV, and 10% to 34% for BSV. Bovine lymphotrophic herpesvirus and BSV were also found in a few (1-4 of 21 tested) cases of terminal SA-MCF, but BIV and BVDV were not found in either the dairy cows sampled, or in the cases of SA-MCE No significant correlation was found between the presence of any 2 viruses (OHV2, BHV4, BLHV, BSV) in the dairy cows or terminal cases of SA-MCE 相似文献
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The influence of undersown green manure crops for fallows – set-aside land – in view of vegetation-development and the dynamic of nitrate
In view of the establishment of rotational fallows - set-aside land - as well as the bridging of the vegetation-free winter period the suitability of different undersown grasses and legumes m winter cereals was investigated in field trials. Festuca rubra developed well in winter barley and winter wheat. The success of the cultivation of Lolium perenne, Trifolium pratense and Trifolium repens in winter cereals is uncertain because of the risk of light and water deficiences. Dactylis glomerata caused an negative influence on the yield of the cover crop. During the fallow period only low levels of nitrate could be found in the soil. Under fallow tilled repeatedly complete fallow- a nitrate accumulation could be observed during the fallow period. After ploughing up the fallow vegetation, nitrate concentrations - depth 0–150 cm – increased to 60 kg NO3 -N · ha-1 after the natural fallow without seeding, between 60 and 130 kg NO3 -N · ha-1 after Trifolium and between 160 and 210 kg NO3 -N · ha-1 after complete fallow. Lowest nitrate levels were observed from the grass-sites. Undersown Festuca rubra seems to be the best choice in order to compete with weeds, to form a dense sod and to prevent nitrate leaching. In general a seed rate of 8 kg · ha-1 can be recommended. 相似文献
In view of the establishment of rotational fallows - set-aside land - as well as the bridging of the vegetation-free winter period the suitability of different undersown grasses and legumes m winter cereals was investigated in field trials. Festuca rubra developed well in winter barley and winter wheat. The success of the cultivation of Lolium perenne, Trifolium pratense and Trifolium repens in winter cereals is uncertain because of the risk of light and water deficiences. Dactylis glomerata caused an negative influence on the yield of the cover crop. During the fallow period only low levels of nitrate could be found in the soil. Under fallow tilled repeatedly complete fallow- a nitrate accumulation could be observed during the fallow period. After ploughing up the fallow vegetation, nitrate concentrations - depth 0–150 cm – increased to 60 kg NO
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J L Traub-Dargatz P C Schultheiss M L Kiper T S Stashak R Wrigley J Schlipf F M Applehans 《Journal of the American Veterinary Medical Association》1992,201(4):603-607
Fibrosis of the small intestine led to recurrent colic and weight loss in 2 ponies and 5 horses. There was a reduction in the length of the small intestine to one-half normal in horses 4 to 7. Histologic examination revealed substantial small intestinal submucosal fibrosis and arteriole sclerosis. The cause was not determined, but an environmental factor was suspected because 3 horses were from the same farm and the other animals were from within a 10-mile radius of the farm. The submucosal fibrosis appeared to be secondary to sclerosis of arterioles in the submucosa and mesentery, with low blood flow state or altered vessel permeability. 相似文献
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Tryon RC White SD Famula TR Schultheiss PC Hamar DW Bannasch DL 《American journal of veterinary research》2005,66(3):437-442
OBJECTIVE: To assess heritability and mode of inheritance for hereditary equine regional dermal asthenia (HERDA) in Quarter Horses. ANIMALS: 1,295 horses with Quarter Horse bloodlines, including 58 horses affected with HERDA. PROCEDURE: Horses were classified as affected or unaffected or as undetermined when data were insufficient to assess phenotype. Pedigree data were analyzed to determine the probable mode of inheritance. Heritability was estimated by use of Bayesian statistical methods. RESULTS: Heritability (mean+/-SD) of HERDA was estimated to be 0.38+/-0.13, with both sexes having an equal probability of being affected. Results for evaluation of the pedigrees were consistent with a single Mendelian autosomal recessive mode of inheritance. CONCLUSIONS AND CLINICAL RELEVANCE: HERDA in Quarter Horses is an inherited disease, and affected horses are more likely to produce affected offspring. An autosomal recessive mode of inheritance should be considered by people making breeding decisions involving Quarter Horses when a first-degree relative has been confirmed with HERDA or has produced affected offspring. In addition, breeders whose horses have produced affected offspring can reduce the likelihood of producing affected horses in the future by avoiding inbreeding. 相似文献
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Fenimore A Varanat M Maggi R Schultheiss P Breitschwerdt E Lappin MR 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(3):613-616
Background: Several Bartonella species (spp.) have been identified in dogs diagnosed with infectious endocarditis (IE) or myocarditis. Objective: To interrogate cardiac tissues of dogs with suspected IE for the presence of Bartonella spp. DNA of dogs in the Rocky Mountain states. Animals: Nine dogs with a clinical diagnosis of endocarditis from January 1990 to June 2008 were included. Methods: In this retrospective study, medical records at the Veterinary Teaching Hospital were searched. Animals were excluded if there was no diagnosis of IE in the original necropsy report. Paraffin embedded tissue blocks and medical records were available from 9 dogs. Total DNA was extracted from the cardiac tissues and assessed for Bartonella spp. DNA by 3 polymerase chain reaction (PCR) methods. For positive samples, the Bartonella spp. were determined by genetic sequencing or fluorogenic real‐time PCR. Results: Bartonella henselae DNA was amplified from the tissues of 7 dogs; Bartonella vinsonii subsp berkhoffii DNA was amplified concurrently from 3 dogs. Six dogs were from Colorado and 1 was from Wyoming. Flea or tick infestations were reported in 2 dogs. Conclusions and Clinical Importance: Bartonella spp. should be on the differential list for dogs in the Rocky Mountain states. The results emphasize the need for routine use of external parasite control products even in regions perceived to have low risk for flea and tick infestations. 相似文献
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Mucopolysaccharidosis VII was diagnosed in a domestic shorthair cat from California. The cat was small and had multiple abnormalities, including a small body disproportionate to the size of the skull, angular deformities of the ribs, abnormally short forelimbs, luxating patellas, generalized epiphyseal dysplasia involving the vertebrae and long bones, cuboidal vertebrae, pectus excavatum, subluxation of both hips, osteosclerosis of the tentorium cerebelli and left petrous temporal bone, tracheal hypoplasia, and corneal clouding. Beta-glucuronidase activity was markedly decreased in peripheral blood leukocytes. The cat died at 21 months of age, and a complete necropsy was performed. Tissues were examined by light and transmission electron microscopy. Large clear, round vacuoles representing distended lysosomes were present in many epithelial and connective tissue cells, including fibrocytes, chondrocytes, smooth muscle cells, hepatocytes, astrocytes, and macrophages. 相似文献
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White SD Affolter VK Schultheiss PC Ball BA Wessel MT Kass P Molinaro AM Bannasch DL Ihrke PJ 《Veterinary dermatology》2007,18(1):36-40
A Quarter horse filly bred from two horses affected with HERDA (hereditary equine regional dermal asthenia) was observed clinically and its skin histologically for the 1.5 years of its life. Severe signs of the disease did not manifest until 1.5 years of age, and were not temporally related to saddling. Histological comparison to an age-, breed- and sex-matched control did not show any consistent diagnostic features. Monitoring of the proband substantiated previous reports of (i) the autosomal recessive nature of the disease, (ii) mares affected with HERDA being able to foal without damage to the skin or reproductive tract, (iii) HERDA foals appearing phenotypically normal throughout the first year of life, and (iv) demonstrated that histological interpretation of skin specimens from grossly normal skin may be insufficient to differentiate HERDA-affected horses from controls. 相似文献
20.
P C Schultheiss J K Collins T R Spraker J C DeMartini 《Journal of veterinary diagnostic investigation》2000,12(6):497-502
Three bison herds in Colorado experienced high mortality from malignant catarrhal fever (MCF). In comparison with cattle, the bison had a more rapidly progressive disease, fewer clinical signs, and milder inflammatory histologic lesions. There was consistent association with ovine herpesvirus-2 (OHV-2). Contact with sheep was not consistent. Of 17 animals in herd A, 15 died of acute MCF; 1 was slaughtered while healthy; and 1 developed clinical signs of MCF, was treated with corticosteroids and antibiotics, and died of fungal abomasitis and rhinitis after 5 months. In herds B and C, approximately 300 of 900 and 18 of 20 died of MCF following brief clinical disease. The nearest sheep were 1 mile away from herd A, but direct contact with sheep could be documented in herds B and C. Complete gross and histologic examinations were conducted on 34 animals, including all animals in herd A, and MCF was diagnosed in 31. In addition, field necropsies were performed on all dead animals in herd B and most in herd C and MCF was diagnosed on the basis of the gross lesions in most animals. Clinical signs of each animal in herd A were recorded. Illness was brief, usually 8-48 hours. Clinical signs were subtle; separation from the herd was often observed. In all 3 herds, hemorrhagic cystitis and multifocal ulceration of the alimentary tract were consistently found at necropsy. Mild lymphocytic vasculitis was present in multiple organs. Ovine herpesvirus-2 was found by polymerase chain reaction (PCR) in 71 of 105 formalin-fixed tissue specimens from 29 of 31 animals with MCF. In herd A, blood samples from 13 animals were collected at 5 time points and tested by PCR for the presence of OHV-2 viral sequences in peripheral blood leukocytes. Nine bison with a positive PCR test and 4 with negative results prior to clinical illness died of MCF. 相似文献