Biocontrol efficiency of Fusarium wilt diseases by a root-colonizing fungus Penicillium sp.     

Syed Sartaj Alam  Kazuyuki Inubushi 《Soil Science and Plant Nutrition》2013,59(2):204-212

Soil-inhabiting fungal pathogen Fusarium oxysporum often causes severe yield losses in many crops. We investigated the effect of a plant growth-promoting fungus, Penicillium sp. EU0013 on Fusarium wilt disease. In dual culture experiments, EU0013 inhibited the growth of Fusarium wilt pathogens by producing an inhibition zone. In experiments using sterile potting medium under controlled conditions, EU0013 significantly reduced the severity of Fusarium wilt on tomato (Solanum lycopersicum L.) and cabbage (Brassica oleracea L. var. capitata). In non-sterile soil, benomyl-resistant mutants of EU0013 were selected by exposing the conidial solution of EU0013 to ultraviolet light. The selected mutant EU0013_90S isolate did not show any distinct differences from EU0013 in colony characteristics, growth rate or antifungal activity against Fusarium wilt pathogens in dual culture. The effect of EU0013_90S on tomato wilt was studied under greenhouse conditions using non-sterile soil. Two-weeks old tomato seedlings were dipped in four different concentrations of EU0013_90S conidial suspension (1?×?10³, 1?×?10⁴, 1?×?10⁵, and 1?×?10⁶ conidia mL^–1). Seedlings were then planted in soil inoculated with either F. oxysporum f. sp. lycopersici race 1 CU1 or race 2 JCM 12575 (1?×?10⁶ bud-cells g^–1). We found the greatest disease suppression occurred when seedlings were dipped in the highest concentration of EU0013_90S conidia. This same inoculum concentration of EU0013_90S also resulted in the highest disease reduction in soil infested with JCM 12575. Higher root colonization with EU0013_90S showed a significant reduction in Fusarium wilt disease, suggesting that colonization by Penicillium sp. EU0013_90S is important for efficient biocontrol of these diseases.  相似文献   

A Simple PCR Procedure to Detect White Spot Syndrome Virus (WSSV) of Shrimp, <Emphasis Type="Italic">Penaeus monodon</Emphasis> (Fabricious)     

A.S. Sahul?HameedEmail author  V.?Parameswaran  S. Syed?Musthaq  R.?Sudhakaran  G.?Balasubramanian  K.?Yoganandhan 《Aquaculture International》2005,13(5):441-450

A non-stop, single tube and semi-nested polymerase chain reaction (PCR) technique with simple procedure was developed for simultaneous detection and grading the level of white spot syndrome infection in penaeid shrimp, Penaeus monodon. In this PCR procedure, three sense primers and one antisense primer with uniform annealing temperature of 55 °C were used. These primers amplify three PCR products (500, 300 and 200 base pairs [bp]) depending upon the severity of infection. Quantities of WSSV-DNA at 10 pg and greater gave three PCR products of 500, 300, 200 bp. A moderate concentration of WSSV-DNA, around 100 fg, gave two products of 300 and 200 bp and a low concentration of 1 fg or more gave only one PCR product of 200 bp. This PCR technique was assessed for early detection of WSSV in shrimp. In time-course infectivity experiments conducted on shrimp with WSSV, one PCR product (200 bp) was seen in hemolymph, tail tissue and gill at 3 h post infection (p.i.); two PCR products (300 and 200 bp) were seen in tail tissue, hemolymph, heart tissue and gill at 18 h p.i. At 30 h p.i., three PCR products (500, 300, 200 bp) were seen in all the organs tested. The samples collected from control animals showed negative for WSSV.  相似文献   

Hexane Extract of Raphanus sativus L. Roots Inhibits Cell Proliferation and Induces Apoptosis in Human Cancer Cells by Modulating Genes Related to Apoptotic Pathway     

Syed Sultan Beevi  Lakshmi Narasu Mangamoori  Murugan Subathra  Jyotheeswara Reddy Edula 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(3):200-209

Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. It has been used in indigenous system of medicine for the treatment of various human ailments in India. This present study evaluated the chemopreventive efficacy of different parts of R. sativus such as root, stem and leaves, extracted with solvents of varying polarity and investigated the molecular mechanism leading to growth arrest and apoptotic cell death in human cancer cell lines. Of the different parts, significant growth inhibitory effect was observed with hexane extract of R. sativus root. Analysis of hexane extract by GC-MS revealed the presence of several isothiocyanates (ITCs) such as 4-(methylthio)-3-butenyl isothiocyanate (MTBITC), 4-(methylthio)-3-butyl isothiocyanate (erucin), 4-methylpentyl isothiocyanate, 4-pentenyl isothiocyanate and sulforaphene. R. sativus root extract induced cell death both in p53 proficient and p53 deficient cell lines through induction of apoptotic signaling pathway regardless of the p53 status of cells. The molecular mechanisms underlying R. sativus-induced apoptosis may involve interactions among Bcl₂ family genes, as evidenced by up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic genes along with activation of Caspase-3. Our findings present the first evidence that hexane extract of R. sativus root exerts potential chemopreventive efficacy and induces apoptosis in cancer cell lines through modulation of genes involved in apoptotic signaling pathway.  相似文献   

Effect of hot acetic acid solutions on postharvest decay caused by Penicillium expansum on Red Delicious apples     

Mohsen Radi  Hassan Afshari Jouybari  Gholamreza Mesbahi  Asgar Farahnaky  Sedigheh Amiri 《Scientia Horticulturae》2010

The objective of this research was improvement of apple hot water treatment efficiency by using acetic acid. The apples (cultivar Red Delicious) were treated using hot acetic acid solutions (1, 2 and 3%) at 50 °C for 1, 2 and 3 min. The results of in vitro study showed that treatment with acetic acid at 50 °C can significantly reduce the growth of Penicillium expansum spores. The treatment of apples with 50 °C acetic acid solutions, in particular 2% acetic acid solution for 3 min or 3% acetic acid solution for 2 min, had significant impacts in reducing the extent of decay of the fruit during the short time storage experiment, while this effect was not significant in the long-time storage.  相似文献   

Evaluation of the effects induced by dietary diphenyl diselenide on common carp Cyprinus carpio     

Charlene Menezes  Jossiele Leitemperger  Adriana Santi  Glaecir Dias  Fábio Araújo Pedron  João Radünz Neto  Syed Mahammad Salman  Nilda Berenice Vargas Barbosa  Vania Lucia Loro 《Fish physiology and biochemistry》2014,40(1):141-149

Several diets employed in aquaculture are enriched with selenium (Se), as it is a fundamental element to aquatic vertebrates. Diphenyl diselenide [(PhSe)₂], which is a synthetic organoselenium compound, has been considered a potential antioxidant agent in different experimental models. Thus, the aim of this study was to evaluate the effects of dietary diphenyl diselenide at concentrations of 1.5, 3.0, and 5.0 mg/kg for 60 days and to determine its optimal supplemental level for carp, Cyprinus carpio. Neither growth retardation nor hepatoxicity was induced by the inclusion of diphenyl diselenide at concentrations ranging from 1.5 to 5.0 mg/kg. In addition, the inclusion of 3.0 mg/kg of diphenyl diselenide stimulated the weight and length of the carp. The supplementation with 1.5 and 3.0 mg/kg of diphenyl diselenide did not produce oxidative damage in the tissues, verified by peroxidation lipid and protein carbonyl assays. However, at 5.0 mg/kg, it caused an increase of the lipid peroxidation in the liver, brain, and muscle, and inhibited the cerebral acetylcholinesterase activity. An increase of the hepatic superoxide dismutase activity and non-protein thiols content in all tissues and ascorbic acid in the liver, gills, and brain was verified in carp fed with the diet containing 3.0 mg/kg of diphenyl diselenide. This diet had advantageous effects for the fish used in experiments. Therefore, this compound could be considered a beneficial dietary supplement for carp nutrition.  相似文献   

Tree Culture of Smallholder Farmers Practicing Agroforestry in Gunung Salak Valley,West Java,Indonesia     

Syed Ajijur Rahman  Terry Sunderland  James M. Roshetko  Imam Basuki  John R. Healey 《Small-Scale Forestry》2016,15(4):433-442

This paper investigates the types of agroforestry system that exist in Gunung Salak Valley, West Java, Indonesia in order to characterize the differences in their basic structure and associated crop plant diversity. Data were collected through rapid rural appraisal, field observation and focus groups, followed by household survey of a sample of 20 agroforestry farmers. Five main agroforestry systems (homegardens, fruit tree system, timber tree system, mixed fruit–timber system, and cropping in the forest understory) exist in the study area, and all of them exhibit a noticeable diversity in terms of both species composition and utilization. Products from farming accounted for an average 24 % of household income. They comprised agroforestry products which contributed IDR 3.25 million/year and other agricultural products contributing IDR 1.66 million/year. The observed agroforestry systems include not only a form of forest dominated by ‘cultivated trees’, but also an anthropogenic vegetation formation derived from agricultural antecedents. In land-use classifications agroforestry systems are not recognized as forestry, but like forests they provide tree products and services. Classification will always be disfunctional if a binary system is applied, thus a more sophisticated approach should be adopted that incorporates the economic and environmental characteristics of a wider range of systems.  相似文献   

Development of breeding populations from interspecific hybrids between Trifolium repens L. and T. occidentale Coombe          下载免费PDF全文

Syed Wajid Hussain  Isabelle Marjorie Verry  Warren Mervyn Williams 《Plant Breeding》2016,135(1):118-123

Trifolium occidentale is a diploid wild relative of T. repens (white clover) with adaptation to dry, saline coastal habitats. Transfer of drought and salt‐tolerant adaptive traits to white clover could be potentially valuable if interspecific hybridization can be achieved efficiently and leads to fertile hybrid populations. To achieve hybridization, 4x plants of T. occidentale were generated. Efficient techniques for generation of 4x plants and their identification using dry pollen shape are described. Interspecific 4x F₁ plants were achieved without embryo rescue. F₂ populations and first backcross hybrids to white clover were also efficiently achieved. Although male and female fertility were lower than in white clover, they were adequate to produce large amounts of seed from small numbers of inflorescences. Thus, early generation interspecific hybrid prebreeding populations can be readily developed, opening the way for transfer of traits from T. occidentale to white clover.  相似文献   

QTLs for oil yield components in an elite oil palm (Elaeis guineensis) cross     

Tzer-Ying Seng  Enrique Ritter  Siti Hawa Mohamed Saad  Ling-Jiun Leao  Rajinder Singh Harminder Singh  Faridah Qamaruz Zaman  Soon-Guan Tan  Sharifah Shahrul Rabiah Syed Alwee  Vengeta Rao 《Euphytica》2016,212(3):399-425

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Does acidification of a soil biofilter compromise its methane-oxidising capacity?     

Rashad Syed  Surinder Saggar  Kevin Tate  Bernd H. A. Rehm 《Biology and Fertility of Soils》2016,52(4):573-583

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Isolation and characterization of influenza A virus (subtype H5N1) that caused the first highly pathogenic avian influenza outbreak in chicken in Bhutan     

Dubey SC  Dahal N  Nagarajan S  Tosh C  Murugkar HV  Rinzin K  Sharma B  Jain R  Katare M  Patil S  Khandia R  Syed Z  Tripathi S  Behera P  Kumar M  Kulkarni DD  Krishna L 《Veterinary microbiology》2012,155(1):100-105

We characterized Influenza A/H5N1 virus that caused the first outbreak of highly pathogenic avian influenza (HPAI) in chickens in Bhutan in 2010. The virus was highly virulent to chicken, killing them within two days of the experimental inoculation with an intravenous pathogenicity index (IVPI) of 2.88. For genetic and phylogenetic analyses, complete genome sequencing of 4 viral isolates was carried out. The isolates revealed multiple basic amino acids at their hemagglutinin (HA) cleavage site, similar to other "Qinghai-like" H5N1 isolates. The receptor-binding site of HA molecule contained avian-like amino acids ((222)Q and (224)G). The isolates also contained amino acid residue K at position 627 of the PB2 protein, and other markers in NS 1 and PB1 proteins, highlighting the risk to mammals. However, the isolates were sensitive to influenza drugs presently available in the market. The sequence analysis indicated that the Bhutan viruses shared 99.1-100% nucleotide homology in all the eight genes among themselves and 2010 chicken isolate from Bangladesh (A/chicken/Bangladesh/1151-11/2010) indicating common progenitor virus. The phylogenetic analysis indicated that the Bhutan isolates belonged to sub-clade 2.2.3 (EMA 3) and shared common progenitor virus with the 2010 Bangladesh virus. Based on the evidence of phylogeny and molecular markers, it could be concluded that the outbreaks in Bhutan and Bangladesh in 2010 were due to independent introductions of the virus probably through migratory birds.  相似文献