Unilateral basement membrane zone alteration of the regenerated laminar region in equine chronic laminitis   总被引：1，自引：0，他引：1  

Kuwano A  Ueno T  Katayama Y  Nishiyama T  Arai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(7):685-691

Between the laminar epidermis and the laminar dermis of laminar region (LR) in equine foot, it can be observed the basement membrane zone (BMZ), which is composed of a basement membrane and its accompaniments like the hemidesmosome and anchoring fibril. Alteration in the BMZ in equine laminitis is possibly related with not only development but also recovery outcome and recurrence of this disease. However, there is little known about the structure of the BMZ during the recovery phase of this disease. To assess the condition of the BMZ of LR affected by chronic laminitis, the tissue was examined in three cases at two weeks, four weeks and three months after the onset of laminitis, using pathological, immunohistochemical and electron microscopic techniques. Histologically in all laminitis cases, there was a regenerated laminar epidermis with proliferating keratinocytes between the Stratum medium and the dermis, but it included the undeveloped secondary epidermal laminae (ud-SELs) structure in one side of the primary epidermal laminae, especially in the part of the deep area of LR. Immunohistochemical results were positive for the anti-type IV collagen, anti-type VII collagen and anti-laminin 5 antibodies in the most BMZs. However, partial BMZs adjacent to the ud-SELs were negative for the anti-type VII collagen and anti-laminin 5 antibodies. Ultrastructurally, in the BMZ of the ud-SEL, the lamina densa and the lamina lucida were present. In contrast, the anchoring fibrils and the hemidesmosomes were either absent, or present at lower than normal levels. In conclusion, the present study indicated that the part of regenerated LR in chronic laminitis was not able to fully restore to construct the BMZ for a long time, especially in the unilateral side of laminar epidermis. It might be related with recurrence of this disease.  相似文献   

Simultaneous down-regulation of DORMANCY-ASSOCIATED MADS-box6 and SOC1 during dormancy release in Japanese apricot (Prunus mume) flower buds     

Yuto Kitamura  Takanori Takeuchi  Ryutaro Tao 《The Journal of Horticultural Science and Biotechnology》2016,91(5):476-482

In temperate deciduous fruit crops such as Prunus spp., bud endodormancy is an important physiological phase affecting the timing of blooming and subsequent fruit development. Japanese apricot (Prunus mume) bears unmixed flower buds, separate from vegetative buds, that bloom slightly more than a month before vegetative bud burst. Seasonal expression of Prunus mume DORMANCY ASSOCIATED MADS-box genes (PmDAMs) has previously been analyzed only in vegetative buds, with an association between these genes and flower bud endodormancy release not yet confirmed. In this study, we performed a seasonal expression analysis of PmDAM1–6 genes in flower buds of two Japanese apricot genotypes – namely, high-chill and low-chill cultivars. The analysis revealed that PmDAM3, PmDAM5, and PmDAM6 expressions are closely associated with dormancy release in both flower and vegetative buds. In addition, a yeast two-hybrid screening demonstrated that PmDAM6 can interact in yeast with the homolog of Arabidopsis SOC1 (PmSOC1). Synchronized expression patterns were detected in PmDAM6 and PmSOC1 during dormancy release in flower buds of the two genotypes. Taken together, these results suggest that the dimer of PmDAM6 and PmSOC1 may play a role in the regulation of dormancy transition and blooming time in Japanese apricot flower buds.  相似文献   

Quality of Internet information related to the Mediterranean diet     

Hirasawa R  Saito K  Yachi Y  Ibe Y  Kodama S  Asumi M  Horikawa C  Saito A  Heianza Y  Kondo K  Shimano H  Sone H 《Public health nutrition》2012,15(5):885-893

  相似文献   

Export rate of dissolved organic matter from paddy fields during cultivation-activity events     

Sooyong Shim  Bomchul Kim  Yoshihiko Hosoi  Takanori Masuda 《Paddy and Water Environment》2005,3(4):211-218

The aim of this study is to quantify the dissolved organic carbon (DOC) of drainage water from paddy fields in agricultural areas of Tottori prefecture, Japan. In four experimental paddy fields, DOC concentration varied much from 1.1 to 10.1 mg C l⁻¹, and was the highest during heavy runoff that occurred in April when there was a non-agricultural period. However, variation in DOC concentration did not always correspond to rainfall, but depended more on cultivation-activity events such as tilling, planting, draining in summer, and final draining in autumn. The water discharge rate from each experimental field was estimated by using a hydrologic model (the Tank Model and a genetic algorithm). Daily DOC export rate per unit area of three experimental paddy fields was calculated to be 0.0074, 0.0052, and 0.0081 kg a⁻¹ day⁻¹, respectively. The daily DOC export rate showed large seasonal variation with the highest value in May and June. It can be concluded that DOC export from paddy fields can be a substantial source of DOC in receiving waters, and the export rate depends much on cultivation method practice. It might be suggested that DOC export from paddy fields can be controlled by a better water management practice of farmers.  相似文献   

Pneumocystis Pneumonia in a Thoroughbred Racehorse     

Takanori Ueno  Hidekazu Niwa  Yuta Kinoshita  Yoshinari Katayama  Seiji Hobo 《Journal of Equine Veterinary Science》2014

Pneumocystis pneumonia is an opportunistic respiratory infection that occurs in immunocompromised animals. In horses, pneumocystic pneumonia is observed mostly in foals and often progresses rapidly. Here, we report pneumocystic pneumonia in a Thoroughbred racehorse. A 3-year-old Thoroughbred racehorse colt had marked respiratory symptoms for 3 weeks and was unresponsive to antibiotic treatment. At necropsy, firm, tan, patchy lesions were scattered diffusely in the lungs. Microscopically, alveolar septa thickened by proliferation of collagen fibers and infiltration of inflammatory cells were observed. In the alveolar spaces, many brown-black yeast-like organisms similar to cystic forms of Pneumocystis carinii were recognized by staining with Gomori's methenamine silver. Bronchoalveolar lavage fluid (BALF) obtained before necropsy included macrophages engulfing the fungus bodies. Amplified products were obtained from BALF and lung tissue samples by Pneumocystis-specific nested PCR. Phylogenetic analysis based on the 18S rRNA gene sequence revealed that the P. carinii organism from BALF was related to the Pneumocystis spp. detected in other animals and was especially close to P. carinii derived from ferrets. This is a rare case of pneumocystic pneumonia in a colt with chronic pulmonary lesions.  相似文献   

Ingested delphinidin-3-rutinoside is primarily excreted to urine as the intact form and to bile as the methylated form in rats   总被引：2，自引：0，他引：2  

Matsumoto H  Ichiyanagi T  Iida H  Ito K  Tsuda T  Hirayama M  Konishi T 《Journal of agricultural and food chemistry》2006,54(2):578-582

Many reports have described the bioavailability of anthocyanins; however, most of these reports investigated only the amount of anthocyanins excreted in urine. In the present study, we calculated the pharmacokinetic bioavailability of anthocyanins in rats by measuring the plasma concentration of delphinidin-3-rutinoside that had been administered orally or intravenously. Delphinidin-3-rutinoside was primarily absorbed in the blood and excreted into urine as unmetabolized forms with a T(max) of 26.3 min and a C(max) of 0.285 +/- 0.071 micromol/L. We detected small amounts of the metabolite 4'-O-methyl-delphinidin-3-rutinoside in the plasma, but we detected neither anthocyanidin (aglycone) nor glucuro- or sulfoconjugates. For the 8 h period after intake, delphinidin-3-rutinoside and 4'-O-methyl-delphinidin-3-rutinoside were excreted to urine at 795 +/- 375 and 12.3 +/- 2.91 nmol, respectively. Relative to intravenous injection, oral administration of delphinidin-3-rutinoside resulted in complete bioavailability (0.49 +/- 0.06%). Analysis of delphinidin-3-rutinoside plasma concentrations in bile cannulated rats revealed that, for the 8-h period after intake, the intact delphinidin-3-rutinoside excretion ratio in bile was 11% of the excretion ratio of 4'-O-methyl-delphinidin-3-rutinoside, 1.91 +/- 0.35 nmol versus 17.4 +/- 8.67 nmol, respectively. Setting the bile duct cannulation in a Bollman-type cage, however, significantly increased the bioavailability of orally administered delphinidin-3-rutinoside (18.14 +/- 6.24%). This effect appears to stem immobilization stress by reducing gastrointestinal motility. The cumulative excretion of delphinidin-3-rutinoside and 4'-O-methyl-delphinidin-3-rutinoside in urine and bile was 2.67 +/- 1.24% (w/w) of the dose ingested. Studies report that several metabolites are formed after oral ingestion of anthocyanins. Examples include glucuronyl from cyanidin-3-glucoside and both glucuronyl and sulfate conjugates from pelargonidin-3-glucoside. Our results indicate that delphinidin-3-rutinoside might be metabolized differently from cyanidin-3-glucoside and pelargonidin-3-glucoside.  相似文献   

Effects of 5'-uridylic acid feeding on postprandial plasma concentrations of metabolites and metabolic hormones in pre-weaning goats     

Yoshihisa OHTANI  Yosuke KOBAYASHI  Tatsuyuki TAKAHASHI  Satoshi HAGA  Mutsuo TAKAGI  Takanori MASHIKO  Kazuo KATOH  Yoshiaki OBARA 《Animal Science Journal》2009,80(5):570-576

5'-Uridylic acid (UMP), which is present at high concentrations in cow's colostrum, has been shown to cause a reduction in increased plasma levels of insulin and glucose after ingestion of milk replacer in pre-weaning calves. However, the precise mechanisms of UMP action have not been investigated, and its action has not been investigated in other pre-weaning ruminants. In order to demonstrate whether UMP causes changes in postprandial metabolic and hormonal parameters in pre-weaning goats, 11 Saanen kids were given milk replacer (twice a day) without ( n  = 5) or with ( n  = 6) UMP (1 g for each meal, 2 g/day for each head) for 14 days. Analysis of blood samples taken in the morning of day 14 demonstrated that the feeding of milk replacer with UMP abolished the significant changes in postprandial plasma glucose, NEFA, GH and insulin concentrations induced by feeding of milk replacer alone, and demonstrated a tendency to increase IGF-I levels. However, there was no significant difference between the two groups at any sampling time. We conclude that UMP feeding with milk replacer showed a tendency to blunt the postprandial changes in levels of some plasma metabolites and hormones that are induced by replacer alone in pre-weaning goats.  相似文献   

Effect of skeletal muscle decorin on collagen fibrillogenesis in vitro     

Takanori NISHIMURA  Ayaka TANEICHI  Jun-ichi WAKAMATSU  Akihito HATTORI 《Animal Science Journal》2003,74(5):399-405

The effect of skeletal muscle decorin on collagen fibrillogenesis was investigated, in order to provide background for understanding the functions of decorin in skeletal muscle. The self‐assembly of type I and III collagen with the addition of decorin or the core protein of decorin from bovine neonatal skeletal muscle was monitored using a spectrophotmeter. Time course changes in the absorbance of collagen solutions showed typical sigmoidal curves composed of three phases. The time of the initial phase was not different between the collagen solution with decorin and that without decorin. The increase rate of the absorbance in the second phase decreased with concentration of decorin added in collagen solutions. Similar effects on fibrillogenesis of type I and III collagens were observed when the core protein of decorin was added in collagen solutions. These results suggest that regulation of collagen fibrillogenesis by decorin depends on its core protein. The networks of reconstructed collagen fibrils with decorin were looser than those without decorin. Bovine skeletal muscle decorin could participate in the regulation of collagen fibrillogenesis and in the arrangement of collagen fibrils in the intramuscular connective tissue.  相似文献   

Construction of a linkage map for watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) using random amplified polymorphic DNA (RAPD)     

Toshiharu Hashizume  Ikuhiro Shimamoto  Yoshiaki Harushima  Mamiko Yui  Takanori Sato  Tsuyoshi Imai  Masashi Hirai 《Euphytica》1996,90(3):265-273

Summary A linkage map for watermelon (Citrullus lanatus) was constructed on the basis of RADP, ribosomal DNA restriction fragment length polymorphism (RFLP), isozyme, and morphological markers using F₁BC1. A segregating population of 78 individuals was the result of a backcross of a cultivated inbred line (H-7; Citrullus lanatus; 2n=22) and a wild form (SA-1; C. lanatus; 2n=22), in which the latter was the recurrent (male) parent. A total of 69 RAPD, one RFLP, one isozyme, and three morphological markers was found to segregate in the BC1 population. Linkage analysis revealed that 62 loci could be mapped to 11 linkage groups that extended more than 524 centimorgans (cM), while 12 loci segregated independently of all other markers. The locus for exocarp color was linked to two RAPD markers within a region of 5 cM on linkage group 4. The locus for flesh color was linked to a RAPD marker within a region of 30 cM on linkage group 6. The isozyme marker GOT was located on the linkage group 1. Linkage group 2 contained a locus for ribosomal DNA within 5 cM of a RAPD marker. Half of the RAPD markers on the linkage group 7 displayed severely distorted segregation. The construction of linkage map using molecular markers is necessary for the breeding of watermelon to introduce useful gene of wild watermelon efficiently. However the linkage map that was constructed for the most part on the basis of RAPD markers could not cover significant parts of the genome, the linkage map provides breeders of watermelons the possibility of tagging useful agronomic traits, as well as the gene for exocarp color.Abbreviations RAPD random amplified polymorphic DNA - RFLP restriction fragment length polymorphism - GOT glutamate oxaloacetate transaminase - MDH malate dehydrogenase - ACP acid phosphatase - 6PGH 6-phosphogluconate dehydrogenase  相似文献   

The Importance of Sr Isotopic Compositions as an Indicator of Acid-Soluble Minerals in Arid Soils in China     

Yoriko Yokoo  Takanori Nakano  Masataka Nishikawa  Hao Quan 《Water, air, and soil pollution》2001,130(1-4):763-768

Aeolian particles originating from arid areas in China, termed Kosa, are considered to be a major source of non-sea-salt Ca, a dominant base cation, in acid precipitation in northeastern Asia. We extracted evaporite minerals (halite, gypsum, and carbonate) from the surface soil collected at eight desert and loess areas in northern China with water and acetic acid. Most ⁸⁷Sr/⁸⁶Sr ratios of the extracted fractions fell in a restricted range (0.7115 ± 0.0015) and differed from those of bulk soils and acid-insoluble minerals (0.712–0.717). The selective dissolution of the soil carbonates in the atmosphere is consistent with two facts: (1) their ⁸⁷Sr/⁸⁶Sr ratios are close to the maximum ⁸⁷Sr/⁸⁶Sr ratios of spring precipitation in Japan, when Kosa activity is marked; and (2) their mole ratios of Mg/Ca (0.15) and Sr/Ca (0.001) are almost identical to those of precipitation in China and Japan when Kosa activity is marked. The ⁸⁷Sr/⁸⁶Sr, Mg/Ca, and Sr/Ca ratios in the acid-soluble components can be used as indexes of Kosa aerosols in the precipitation of the northwestern Pacific.  相似文献