Leptospira in slaughtered fattening pigs in southern Vietnam: presence of the bacteria in the kidneys and association with morphological findings     

Boqvist S  Montgomery JM  Hurst M  Thu HT  Engvall EO  Gunnarsson A  Magnusson U 《Veterinary microbiology》2003,93(4):361-368

One kidney was collected from each of 32 fattening pigs at an abattoir in southern Vietnam in 2001 in order to demonstrate infecting Leptospira serovar and to associate renal macro- and microscopic findings with the presence of renal leptospires. Leptospires were demonstrated in 22 (69%) of the investigated kidneys by immunofluorescence. Multifocal interstitial nephritis (MFIN) and gross renal lesions (white spots) were each demonstrated in 24 (75%) kidneys. Leptospira interrogans serovar bratislava was isolated from one kidney. There was no association between presence of leptospires and MFIN (P=0.19), respectively and white spots (P=0.98), respectively. These data suggest that Leptospira infection is common among fattening pigs in the study area and that these animals may be considered as an occupational human health hazard. It is also suggested that the presence of white spots is an unreliable indicator of the presence of renal leptospires.  相似文献   

A field survey of changes in the composition of corn borer populations in Israel     

Venezia Melamed-Madjar  S. Tam 《Phytoparasitica》1980,8(3):201-204

A survey of corn borer populations in fields of sweet and fodder corn showed thatChilo agamemnon (Bles.) was the main pest of corn in Israel in 1969 and 1970, after which the population size gradually decreased. The population of another pest,Sesamia cretica (Led.), also decreased, probably due to reductions in the sorghum acreage in Israel; the European corn borer,Ostrinia nubilalis (Hb.), was found in very small numbers. On the other hand,Sesamia nonagrioides (Lef.) has gradually increased in number and is now the main pest attacking all corn varieties in Israel. These changes are believed to be due to alterations in agricultural practices — such as irrigation, to changes in the acreage of the host plants, and to the introduction of new varieties.  相似文献   

Lapine rotavirus: preliminary studies on epizoology and transmission.          下载免费PDF全文

M Petric  P J Middleton  C Grant  J S Tam    C M Hewitt 《Canadian journal of veterinary research》1978,42(1):143-147

Antibody to rotavirus was found in sera of domestic rabbits, wild cottontail rabbits and Snowshoe hares. Eight out of 187 stools from a rabbitry contained rotavirus, though no clinical symptoms were apparent. The virus extracted from one such stool infected a five week old seronegative rabbit and led to distention and congestion of the colon, excretion of virus in the stool and a serological response to virus. Human and Simian rotaviruses did not replicate in the rabbits.  相似文献   

First laboratory confirmation of Xylophilus ampelinus in Slovenia*     

T. Dreo  G. Seljak  J. D. Janse  I. Van Der Beld  L. Tjou‐Tam‐Sin  P. Gorkink‐Smits  M. Ravnikar 《EPPO Bulletin》2005,35(1):149-155

Bacterial blight of grapevine is caused by a slow‐growing bacterium Xylophilus ampelinus. It has been suspected to occur in Slovenia on the basis of visual observation of characteristic symptoms in the 1960s. In the present study, symptoms were recorded in an infected vineyard during three consecutive years (2002/2004). Samples from this vineyard were tested by nested‐PCR and isolation of bacteria on media was attempted. In the first year, angular lesions on leaves were highly expressed and an isolate morphologically similar to X. ampelinus was obtained from one sample. It was purified and identified as X. ampelinus using biochemical and nutritional tests, fatty acid analysis, immuno‐fluorescence, nested PCR and partial sequencing of the 16S rRNA gene. The 16S rDNA sequence showed 99–100% homology to known sequences of X. ampelinus strains, including the type strain. Pathogenicity of the isolate was confirmed in tissue‐cultured and potted grapevine plants. In the following two years, symptoms of bacterial blight were only faintly expressed. Using isolation on media and nested‐PCR, 23 and 17 extracts prepared from 10 and 8 grapevines, respectively, were analysed. In 2003, no positive sample was found, but X. ampelinus was again isolated and identified by colony morphology and nested‐PCR in 2004.  相似文献   

Liquid culture as a positive condition to induce and enhance quality and quantity of somatic embryogenesis of Lilium longiflorum     

Duong Tan Nhut  Nguyen Thuy Minh Hanh  Pham Quoc Tuan  Le Thi Minh Nguyet  Nguyen Thi Huyen Tram  Ngo Chung Chinh  Nguyen Hoang Nguyen  Do Nang Vinh 《Scientia Horticulturae》2006

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A novel in vitro hydroponic culture system for potato (Solanum tuberosum L.) microtuber production     

Duong Tan Nhut  Nguyen Hoang NguyenDang Thi Thu Thuy 《Scientia Horticulturae》2006

An innovative in vitro hydroponic culture system used in potato (Solanum tuberosum L.) microtuber production is described in this paper. In vitro potato plantlets, 6–8 cm in height, derived from meristems of potato tubers cultured on 1/2 Murashige and Skoog (MS) nutrient medium after 30 days culture were cut into 1.5 cm stem node segments and used as explants. These stem nodes were cultured in a novel system called in vitro hydroponic culture system containing 1/2 MS medium supplemented with 0.5 μM naphthaleneacetic acid (NAA), 0.3 μM gibberellic acid (GA₃), 3.7 μM adenine sulfate, 10% coconut water, 0.5 g/l activated charcoal, 80 g/l sucrose with or without 8 g l⁻¹ agar. Liquid medium was distributed to the carrier substrates in each storey of the system with the aid of capillary robes. In the present paper, the effects of porous material used as substrate carrier and the number of storeys involved in the culture system on microtuber formation and their morphological characteristics are reported. Cotton layer substrate is more stable for organogenesis of potato microtubers. Microtubers, 3.19 mm in diameter and 49.82 mg in weight, could be harvested from a one-storey in vitro hydroponic culture system containing filter paper as substrate. However, microtubers cropped from three-storey in vitro hydroponic culture system with cotton layer were bigger and weightier than those from three-storey system containing filter paper. The above results of the in vitro hydroponic system examined in this study might open up a new approach in producing potato and other hygrophilous microtuber.  相似文献   

Liver lipid metabolism   总被引：1，自引：0，他引：1  

Nguyen P  Leray V  Diez M  Serisier S  Le Bloc'h J  Siliart B  Dumon H 《Journal of animal physiology and animal nutrition》2008,92(3):272-283

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Quantitative real-time RT-PCR measurement of cytokine mRNA expression in the skin of normal cats and cats with allergic skin disease     

Taglinger K  Van Nguyen N  Helps CR  Day MJ  Foster AP 《Veterinary immunology and immunopathology》2008,122(3-4):216-230

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Explaining Usutu virus dynamics in Austria: model development and calibration     

Rubel F  Brugger K  Hantel M  Chvala-Mannsberger S  Bakonyi T  Weissenböck H  Nowotny N 《Preventive veterinary medicine》2008,85(3-4):166-186

Usutu virus (USUV), a flavivirus of the Japanese encephalitis virus complex, was for the first time detected outside Africa in the region around Vienna (Austria) in 2001 by Weissenb?ck et al. [Weissenb?ck, H., Kolodziejek, J., Url, A., Lussy, H., Rebel-Bauder, B., Nowotny, N., 2002. Emergence of Usutu virus, an African mosquito-borne flavivirus of the Japanese encephalitis virus group, central Europe. Emerg. Infect. Dis. 8, 652-656]. USUV is an arthropod-borne virus (arbovirus) circulating between arthropod vectors (mainly mosquitoes of the Culex pipiens complex) and avian amplification hosts. Infections of mammalian hosts or humans, as observed for the related West Nile virus (WNV), are rare. However, USUV infection leads to a high mortality in birds, especially blackbirds (Turdus merula), and has similar dynamics with the WNV in North America, which, amongst others, caused mortality in American robins (Turdus migratorius). We hypothesized that the transmission of USUV is determined by an interaction of developing proportion of the avian hosts immune and climatic factors affecting the mosquito population. This mechanism is implemented into the present model that simulates the seasonal cycles of mosquito and bird populations as well as USUV cross-infections. Observed monthly climate data are specified for the temperature-dependent development rates of the mosquitoes as well as the temperature-dependent extrinsic-incubation period. Our model reproduced the observed number of dead birds in Austria between 2001 and 2005, including the peaks in the relevant years. The high number of USUV cases in 2003 seems to be a response to the early beginning of the extraordinary hot summer in that year. The predictions indicate that >70% of the bird population acquired immunity, but also that the percentage would drop rapidly within only a couple of years. We estimated annually averaged basic reproduction numbers between R (0)=0.54 (2004) and 1.35 (2003). Finally, extrapolation from our model suggests that only 0.2% of the blackbirds killed by USUV were detected by the Austrian USUV monitoring program [Chvala, S., Bakonyi, T., Bukovsky, C., Meister, T., Brugger, K., Rubel, F., Nowotny, N., Weissenb?ck, H., 2007. Monitoring of Usutu virus activity and spread by using dead bird surveillance in Austria, 2003-2005. Vet. Microbiol. 122, 237-245]. These results suggest that the model presented is able to quantitatively describe the process of USUV dynamics.  相似文献   

Experimental results and clinical impact of using autologous rectus fascia sheath for vascular replacement     

Kóbori L  Németh T  Nagy P  Dallos G  Sótonyi P  Fehérvári I  Nemes B  Görög D  Patonai A  Monostory K  Doros A  Sárváry E  Fazakas J  Gerlei Z  Benko T  Piros L  Járay J  De Jong KP 《Acta veterinaria Hungarica》2008,56(3):411-420

Vascular complications are major causes of graft failure in liver transplantation. The use of different vascular grafts is common but the results are controversial. The aim of this study was to create an 'ideal' arterial interponate for vascular replacements in the clinical field. An autologous, tubular graft prepared from the posterior rectus fascia sheath was used for iliac artery replacement in dogs for 1, 3, 6 and 12 months. Forty-one grafts were implanted and immunosuppression was used in separate groups. The patency rate was followed by Doppler ultrasound. Thirty-seven grafts remained patent, 2 cases with thrombosis and 2 cases with stenosis occurred. There was no evidence of necrosis or aneurysmatic formation. The histological analysis included conventional light microscopic and immunohistochemical examinations for CD34 and factor VIII. The explanted grafts showed signs of arterialisation, appearance of elastin fibres, and smooth muscle cells after 6 months. Electron microscopy showed intact mitochondrial structures without signs of hypoxia. In conclusion, the autologous graft presents acceptable long-term patency rate. It is easy to handle and the concept of beneficial presence of the anti-clot mesothelium until endothelialisation seems to work. The first clinical use was already reported by our group with more than 2 years survival.  相似文献