Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)     

Bhanushree Narayanswami  Bhoopal Singh Tomar  Jameel Akhtar  Tusar Kanti Behera  Ranjith Kumar Ellur  Rama Mithra V. Sevanthi  Jaiswal Sarika  Partha Saha 《Plant Breeding》2023,142(5):700-710

The present investigation was carried out to decipher inheritance of resistance and to identify linked SSR markers for Phomopsis blight resistance in eggplant. An F₂ population comprising 161 plants was developed from the cross of Pusa Kranti and BR-40-7. Genetic analysis was carried out using Chi square test. Artificial inoculation of fruits was carried out using pin prick method, and scoring was done as per the standard scoring scale. The F₂ plants segregated into 92 susceptible (77—highly susceptible, 15—susceptible): 69 resistant (17—highly resistant, 27—resistant, 25—moderately resistant) suggesting complimentary epistasis with ratio of 9:7. To identify the putatively linked markers to resistance gene, parental polymorphic markers were subjected to bulk segregant analysis (BSA), and two markers (emk03O04 and emf11A03) could differentiate resistant and susceptible bulk and co-segregated with resistance gene. The genetic distance between the identified markers was found to be 18.12 cM using QTL IciMapping V3.2 software depicting two new QTLs on chromosome number 6. The identified QTLs have great significant importance in marker assisted breeding programme.  相似文献   

Cytogenetic analysis of wild emmer populations from Turkey and Israel   总被引：3，自引：0，他引：3  

Kanti Rawal  J. R. Harlan 《Euphytica》1975,24(2):407-411

Summary Chromosome behavior during meiosis of the F₁ hybrids involving two races of wild emmer Triticum turgidum var. dicoccoides and var. timopheevi is described. Four of the six Turkish accessions were similar to the Israeli group in pairing relationships and seed set percentages. The collection (# 189) is cytologically similar to var. timopheevi. The other Turkish accession (# 191) showed good chromosome pairing with both var. dicoccoides and var. timopheevi.  相似文献   

Distribution pattern of bovine viral diarrhoea virus type 1 genome in lymphoid tissues of experimentally infected sheep     

M. Karikalan  K. Rajukumar  N. Mishra  M. Kumar  S. Kalaiyarasu  K. Rajesh  V. Gavade  S. P. Behera  S. C. Dubey 《Veterinary research communications》2016,40(2):55-61

In this study, cellular localization and the distribution pattern of BVDV genome in lymphoid tissues during the course of experimental acute BVDV-1 infection of sheep was investigated. Tonsils, mesenteric lymph nodes (MLN) and spleen were collected on 3, 6, 9, 12 and 15 days post infection (dpi) from twenty 4-month-old lambs, experimentally inoculated intra-nasally with 5?×?10⁵ TCID₅₀ of a non-cytopathic (ncp) BVDV-1 isolate, Ind-17555. Tissues collected from ten mock-infected lambs served as controls. In situ hybridization (ISH) was carried out in paraformaldehyde fixed paraffin embedded tissue sections using digoxigenin labelled riboprobe targeting 5′-UTR of BVDV-1. BVDV genome was detected at all the intervals from 3 dpi to 15 dpi in the lymphoid tissues with variations between the intervals and also amongst the infected sheep. During the early phase of acute infection, presence of viral genome was more in tonsils than MLN and spleen, whereas the distribution was higher in MLN during later stages. BVDV-1 genome positive cells included lymphocytes, macrophages, plasma cells, reticular cells and sometimes crypt epithelial cells. Genome distribution was frequently observed in the lymphoid follicles of tonsils, MLN and spleen, besides the crypt epithelium in tonsils, paracortex and medullary sinus and cords of MLN. Most abundant and widespread distribution of BVDV-1 genome was observed on 6 dpi while there was a reduction in number and intensity of positive signals by 15 dpi in most of the infected animals. This is the first attempt made to study the localisation of BVDV-1 in lymphoid tissues of acutely infected sheep by in situ hybridization. The results show that the kinetics of BVDV-1 distribution in lymphoid tissues of experimentally infected non-pregnant sheep follows almost a similar pattern to that demonstrated in BVDV infected cattle.  相似文献   

Estimation of potassium concentration in oil palm (Elaeis guineensis Jacq.) leaf tissue by simple and inexpensive water extraction method     

S. K. Behera  K. Suresh  B. N. Rao  K. Manoja  K. Manorama  K. Ramachandrudu 《Journal of plant nutrition》2016,39(9):1250-1256

Estimation of potassium (K) concentration in oil palm leaf tissue is routinely carried out in oil palm plantations to manage fertilizer application for getting higher fresh fruit bunch (FFB) production. Since K in plant tissue is not bound to organic complexes and it is extractable by water, this study was carried out to extract K from oil palm leaf tissue by water extraction method. The results were compared with other established methods like 1 normal (N) ammonium acetate (NH₄OAc) extraction, 0.5 N hydrochloric acid (HCl) extraction, and diacid digestion. The proposed water extraction method consists of shaking of 0.5 g finely ground oil palm leaf tissue with distilled water at 1:60 ratio [sample-to-water weight (w)/volume (v)] for a period of 20 min in a reciprocating shaker, filtration of the content, and measurement of K concentration in filtrate by flame photometer. The results of analysis of 30 oil palm leaf samples collected from various production systems under different soil types and management practices for K concentration revealed the close agreement of water extraction method with other established methods. The mean value of K extracted by water extraction method was within 1–10% of the K extracted by other established methods. Water-extractable K was significantly correlated with K extracted by other methods and it could be predicted by other methods. The values of standard error and coefficient of variation for K extracted by different methods were very low, which indicated that the water extraction method was comparable with other established methods.  相似文献   

An experimental investigation into the mechanical behaviour of 3D woven fabrics for structural composites     

B. K. Behera  B. P. Dash 《Fibers and Polymers》2014,15(9):1950-1955

3D woven composites provide efficient delamination suppression, enhanced damage tolerance, superior impact, ballistic and blast performance characteristics over 2D fabric laminates. At the same time, this type of composites, having practically straight in-plane fibers, show significantly better in-plane stiffness and strength properties than respective properties of conventional composites. But, how the in-plane elastic and strength characteristics of this type of fabrics compare with respective in-plane properties of equivalent 2D woven fabrics. This paper presents a comprehensive experimental study of the comparison of in-plane tensile, bending, crimp interchange properties of UD, 2D, 3D orthogonal, 3D angle-interlock and 3D warp interlock multi-layer structures woven from E-glass tow. The results depict that the 3D woven fabrics have considerably superior mechanical properties with much lesser crimp compared to 2D fabrics.  相似文献   

Characterizing Shorea robusta communities in the part of Indian Terai landscape     

V. S. Chitale  M. D. Behera  S. Matin  P. S. Roy  V. K. Sinha 《林业研究》2014,25(1):121-128

Shorea robusta Gaertn.f.(Sal)is one of the important timber-yielding plants in India,which dominates the vegetation of Terai landscape of Uttar Pradesh state in India forming various communities based on its associations.The present study deals with delineation,mapping and characterization of various communities of Sal(Shorea robusta)forests in Terai landscape of Uttar Pradesh,India ranging across over 16districts.Field survey and visual interpretation based forest vegetation type classification and mapping was carried out as part of the project entitled‘Biodiversity characterization at landscape level using remote sensing and GIS’.Indian Remote Sensing-P6(Resourcesat-1)Linear Imaging Self Scanner-III satellite data was used during the study.The total area covered by different Sal forests was found to be approximately2256.77 km2.Sal communities were identified and characterized based on their spectral properties,physiognomy and phytosociological characteristics.Following nine Sal communities were identified,delineated and mapped with reasonable accuracy viz.,Chandar,Damar,dry plains,moist plains,western alluvium,western alluvium plains,mixed moist deciduous,mixed dry deciduous and Siwalik.It is evident from the area estimates that mixed moist deciduous Sal is the most dominant community in the region covering around(1613.90 km2),other major communities were found as western alluvium plains Sal(362.44 km2),mixed dry deciduous Sal(362.44 km2)and dry plains Sal(107.71 km2).The Terai landscape of Uttar Pradesh faces tremendous anthropogenic pressure leading to deterioration of the forests.Community level information could be used monitoring the status as well as for micro level conservation and planning of the Sal forests in Terai Landscape of Uttar Pradesh.  相似文献   

Oxidative stress indices in the erythrocytes from lactating cows after treatment for subclinical ketosis with antioxidant incorporated in the therapeutic regime     

Sahoo SS  Patra RC  Behera PC  Swarup D 《Veterinary research communications》2009,33(3):281-290

The present investigation was carried out to assess the erythrocytic oxidative stress indices such as lipid peroxides level and activities of antioxidant enzymes such as superoxide dismutase and catalase, and some hematological parameters after treatment of subclinically ketotic lactating cows with antioxidants, vitamin E and selenium, incorporated in conventional treatment regimen. The study was carried out using lactating cows reared in small dairy herds in and around Bhubaneswar. Out of 250 urine samples examined, 42 cows were diagnosed positive for subclinical ketosis with an overall incidence of 16.8%. Blood samples were collected on day 0 (before treatment) and treatment was initiated on day 1 and the second sampling was carried out on day 7. The hematological parameters improved significantly following treatment as compared to pre-treatment level (day 0). A significant (P < 0.05) improvement was observed on day 7 with respect to hemoglobin level (8.66 ± 0.23 vs. 9.12 ± 0.14 gm/dl) and packed cell volume (29.7 ± 0.56 vs. 31.1 ± 0.50%) in the group given routine treatment along with antioxidants. The comparison of mean lipid peroxides level before (day 0) and after treatment (day 7) revealed a significant difference in group I (routine treatment, P < 0.05) and group II (routine treatment plus antioxidants, P < 0.01). The maximum reduction in SOD activity was recorded in group II animals after treatment with conventional regimes along with supplemented antioxidants (1.29 ± 0.08 against 1.89 ± 0.15 units/ mg of Hb, P < 0.01). The mean value of SOD activity recorded in group III animals kept as non-treated positive control (PC) on day 7 (2.59 ± 0.14 units/mg of Hb) was significantly (P < 0.05) higher than the mean level recorded on day 0 (1.91 ± 0.31 units/mg of Hb). An almost similar trend was observed in erythrocytic catalase activity where there was a significant (P < 0.05) reduction in catalase activity in the group II (RT+A) but there was no significant increase in catalase activity in non-treated positive control (PC) animals.  相似文献   

Non-enzymatic antioxidant status and modulation of lipid peroxidation in the muscles of <Emphasis Type="Italic">Labeo rohita</Emphasis> by sub lethal exposure of CuSO<Subscript>4</Subscript>     

S. D. Jena  M. Behera  J. Dandapat  N. Mohanty 《Veterinary research communications》2009,33(5):421-429

Xenobiotics-mediated environmental stress is an important determining factor in the maintenance of fish health as fishes are frequently exposed to such components. Increasing evidences indicate that acute and chronic xenobiotic exposure modulates ROS production, suppresses immune response and increase the incidence of fish diseases. In the present context an attempt has been made to study the in vivo effect of different concentrations of CuSO₄ (0.5, 1.00 or 2.00 ppm) on lipid peroxidation (an index of oxidative stress) and non enzymatic antioxidant status (glutathione and Ascorbic acid), in the muscle of a widely consumed freshwater fish Labeo rohita. From the out come of this study it is concluded that comparatively low dose of copper (0.5 ppm) induce mild oxidative stress in the experimental fish with concomitant elevation of GSH and AsA content of the muscle. However, high concentration of CuSO₄ (2.00 ppm) in the ambient water leads to severe oxidative stress manifested in the form of LPX and morphoanatomical alteration.  相似文献   

Advances on plant salinity stress responses in the post-genomic era: a review     

Behera  Lalita Mohan  Hembram  Padmalochan 《Journal of Crop Science and Biotechnology》2021,24(2):117-126

Journal of Crop Science and Biotechnology - Plant productivity is significantly affected by several environmental stresses. Excess amount of salt in the soil is one of the environmental stresses...  相似文献   

The effect of tropical forest conversion on soil microbial biomass   总被引：3，自引：0，他引：3  

S. Basu  N. Behera 《Biology and Fertility of Soils》1993,16(4):302-304

We investigated the effects of converting forest to savanna and plough land on the microbial biomass in tropical soils of India. Conversion of the forest led to a significant reduction in soil organic C (40–46%), total N (47–53%), and microbial biomass C (52–58%) in the savanna and the plough land. Among forest, savanna, and plough land, basal soil respiration was maximum in the forest, but the microbial metabolic quotient (qCO₂ was estimated to be at a minimum in the forest and at a maximum in the plough land.  相似文献