Identification of novel quantitative trait loci associated with brown planthopper resistance in the rice landrace Salkathi     

Sangram Keshori Mohanty  Rudraksh Shovan Panda  Soubhagya Laxmi Mohapatra  Arundhati Nanda  Lambodar Behera  Mayabini Jena  Rabindra Kumar Sahu  Sarat Chandra Sahu  Trilochan Mohapatra 《Euphytica》2017,213(2):38

The brown planthopper (BPH) is a potent pest of rice in Asia and Southeast Asia. Host resistance has been found to be the most suitable alternative to manage the insect. But varietal resistance has been found to be short-lived. There has been a constant search for alternate resistance genes. We developed an F₈ recombinant inbred population for the BPH resistance gene in Salkathi, an indica landrace from Odisha, India. Phenotyping of RILs against the BPH population at Cuttack, Odisha showed continuous skewed variation with four peaks at 2.1–3.0, 4.1–5.0, 6.1–7.0 and 8.1–9.0 SES score, suggesting the involvement of quantitative loci for resistance to BPH in Salkathi. Mapping showed the presence of two QTLs on the short arm of chromosome 4. One QTL, with phenotype variance of 37.02% is located between the markers RM551 and RM335. The other QTL, with phenotype variance of 7.1% is located between markers RM335 and RM5633. The two QTLs have been designated as qBph4.3 and qBph4.4. QBph4.3 seems to be a novel QTL associated with BPH resistance. We have successfully transferred qBph4.3 and qBph4.4 into two elite rice cultivars, Pusa 44 and Samba Mahsuri. Fine mapping of the identified QTLs may lead to a successful transfer of QTLs into other elite germplasm backgrounds.  相似文献   

Is the Organic System Economically Viable? The Case of Pineapple in India’s Northeast     

Ujjwal Kanti Paul  Gurudas Das  Avishek Ray  Tanuj Mathur 《International Journal of Fruit Science》2017,17(3):269-279

This study intends to fill the gaps in the organic agriculture literature emerging from infrequent use of farm budget-related data, overlooking the imputed costs and non-existence of long-run return and profitability estimates. The results suggest that the organic system is economically unviable as the growers fail to recover their total production cost. However, growers’ returns exceed their ‘perceived’ production cost, which excludes their imputed expenses. Temporal, spatial, and size-based analysis also attests to this. On the contrary, cultivation in two sample villages where the household’s size, its head’s age, percentage of land, and yield are significantly higher, remain economically viable.  相似文献   

Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses     

Bidhan C. De  D. K. Meena  B. K. Behera  Pronob Das  P. K. Das Mohapatra  A. P. Sharma 《Fish physiology and biochemistry》2014,40(3):921-971

Aquaculture is emerging as one of the most viable and promising enterprises for keeping pace with the surging need for animal protein, providing nutritional and food security to humans, particularly those residing in regions where livestock is relatively scarce. With every step toward intensification of aquaculture practices, there is an increase in the stress level in the animal as well as the environment. Hence, disease outbreak is being increasingly recognized as one of the most important constraints to aquaculture production in many countries, including India. Conventionally, the disease control in aquaculture has relied on the use of chemical compounds and antibiotics. The development of non-antibiotic and environmentally friendly agents is one of the key factors for health management in aquaculture. Consequently, with the emerging need for environmentally friendly aquaculture, the use of alternatives to antibiotic growth promoters in fish nutrition is now widely accepted. In recent years, probiotics have taken center stage and are being used as an unconventional approach that has numerous beneficial effects in fish and shellfish culture: improved activity of gastrointestinal microbiota and enhanced immune status, disease resistance, survival, feed utilization and growth performance. As natural products, probiotics have much potential to increase the efficiency and sustainability of aquaculture production. Therefore, comprehensive research to fully characterize the intestinal microbiota of prominent fish species, mechanisms of action of probiotics and their effects on the intestinal ecosystem, immunity, fish health and performance is reasonable. This review highlights the classifications and applications of probiotics in aquaculture. The review also summarizes the advancement and research highlights of the probiotic status and mode of action, which are of great significance from an ecofriendly, sustainable, intensive aquaculture point of view.  相似文献   

Drought Tolerance in Rice: Focus on Recent Mechanisms and Approaches     

Debabrata PANDA  Swati Sakambari MISHRA  Prafulla Kumar BEHERA 《水稻科学》2021,28(2):119-132

Drought stress is a serious limiting factor to rice production,which results in huge economic losses.It is becoming a more serious issue with respect to the global climate change.Keeping in view of the current and forecasted global food demand,it has become essential to enhance the crop productivity on the drought-prone rainfed lands with priority.In order to achieve the production target from rainfed areas,there is a requirement of rice varieties with drought tolerance,and genetic improvement for drought tolerant should be a high priority theme of research in the future.Breeding for drought tolerant rice varieties is a thought-provoking task because of the complex nature and multigenic control of drought tolerant traits would be a major bottleneck for the current research.A great progress has been made during last two decades in our understanding of the mechanisms involved in adaptation and tolerance to drought stress in rice.In this review,we highlighted the recent progresses in physiological,biochemical and molecular adaptation of rice to drought tolerance.A brief discussion on the molecular genetics and breeding approaches for drought tolerance in rice will be focused for the future crop improvement program for development of drought tolerant rice varieties.  相似文献   

Suitability of Lime,Green Leaf,and Farm Yard Manure and Inorganic Fertilizers for Sustaining Green Gram Yield and Rainwater Use Efficiency Under Moist Sub-Humid Alfisols in India     

Ashok Mishra  B. Behera  A. K. Pal  S. K. Mohanty  B. S. Rath 《Communications in Soil Science and Plant Analysis》2017,48(12):1417-1427

Field experiments were conducted to assess the effect of nutrients management practices on yield and rainwater use efficiency of green gram (Vigna radiata), and soil fertility under moist sub-humid Alfisols at Phulbani, India, during 2005–2008. Ten treatment combinations of lime @ 10% and 20% of lime requirement (LR) @ 8.3 t ha^?1, farmyard manure (FYM) @ 5 t ha^?1, green leaf manure @ 5 t ha^?1, and nitrogen, phosphorus, and potassium (N–P–K) (20–40–20 kg ha^?1) were tested. The analysis of variance indicated that treatments differed significantly from each other in influencing yield and rainwater use efficiency. Application of lime @ 20% LR + FYM @ 5 t/ha + 40 kg P + 20 kg K ha^?1 was superior with maximum mean yield of 531 kg ha^?1, while lime @ 10% LR + FYM @ 5 t ha^?1 + 40 kg P + 20 kg K ha^?1 was the second best with 405 kg ha^?1 and maintained maximum soil fertility of nutrients. The superior treatment gave maximum sustainability yield index of 67.5%, rainwater use efficiency of 0.49 kg ha^?1 mm^?1, improved soil pH, electrical conductivity, and soil nutrients over years.  相似文献   

Soil Carbon Sequestration in Long-Term Fertilization Under Jute-Rice-Wheat Agro-Ecosystem     

A. K. Singh  M. S. Behera  S. P. Mazumdar  D. K. Kundu 《Communications in Soil Science and Plant Analysis》2019,50(6):739-748

Soil organic carbon (SOC) sequestration in response to long-term fertilizer management practices under jute-rice-wheat agro-ecosystem in alluvial soils was studied using a modeling approach. Fertilizer management practices included nitrogen (N), phosphorus (P) and potassium (K) fertilization, manure application, and root-stubble retention of all three crops. Soil carbon (C) model RothC was used to simulate the critical C input rates needed to maintain initial soil C level in long timescale (44 years). SOC change was significantly influenced by the long-term fertilizer management practices and the edaphic variable of initial SOC content. The effects of fertilizer combination “100%NPK+FYM” on SOC changes were most significant over “100%NPK” fertilization. If the 100% NPK fertilizer along with manure applied with stubble and roots retention of all crops, alluvial soils of such agro-ecosystem would act as a net C sink, and the average SOC density kept increasing from 18.18 Mg ha^?1 during 1972 to the current average of ～22 Mg ha^?1 during 2065 s. On an average, the critical C input was estimated to be 5.30 Mg C ha^?1 yr^?1, depending on local soil and climatic conditions. The critical C input could be effectively estimated using a summary model driven by current SOC level, mean annual temperature, precipitation, and soil clay content. Such information will provide a baseline for assessing soil C dynamics under potential changes in fertilizer and crop residues management practices, and thus enable development of management strategies for effectively mitigating climate change through soil C sequestration.  相似文献   

Beta-glucan: an ideal immunostimulant in aquaculture (a review)   总被引：1，自引：1，他引：1  

D. K. Meena  Pronob Das  Shailesh Kumar  S. C. Mandal  A. K. Prusty  S. K. Singh  M. S. Akhtar  B. K. Behera  Kundan Kumar  A. K. Pal  S. C. Mukherjee 《Fish physiology and biochemistry》2013,39(3):431-457

The major hindrance in the development and sustainability of aquaculture industry is the occurrence of various diseases in the farming systems. Today, preventive and management measures are central concern to overcome such outbreak of diseases. Immunostimulants are considered as an effective tool for enhancing immune status of cultured organisms. Among different immunostimulants used in aquaculture practices, β-glucan is one of the promising immunostimulant, which is a homopolysaccharide of glucose molecule linked by the glycoside bond. It forms the major constituents of cell wall of some plants, fungi, bacteria, mushroom, yeast, and seaweeds. Major attention on β-glucan was captivated with the gain in knowledge on its receptors and the mechanism of action. The receptor present inside the animal body recognizes and binds to β-glucan, which in turn renders the animal with high resistance and enhanced immune response. This review highlights β-glucan as an immunostimulant, its effective dosages, and route of administration and furthermore provides an outline on role of β-glucan in enhancing growth, survival, and protection against infectious pathogens pertaining to fishes and shellfishes. Study also summarizes the effect of β-glucan on its receptors, recognition of proteins, immune-related enzymes, immune-related gene expression and their mechanisms of action.  相似文献   

Genotypic variability in plant water status of French bean under drought stress     

Apurba Kanti Choudhury  Abdul Karim  Moynul Haque  Qazi Abdul Khaliq  Jalal Uddin Ahmed  Mofazzal Hossain 《Journal of Crop Science and Biotechnology》2011,14(1):17-24

Seven genotypes of French bean (Phaseolus vulgaris L.) were evaluated under semi-controlled conditions at the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh to analyze genotypic variability in leaf water status under water stress. The plants were grown under two moisture regimes, viz. 80% field capacity (FC) and 50% FC throughout the growing season. The genotypes showed significant variation in water relation traits. Genotypes BB24 and BB43 maintained higher relative water content (RWC), but lower turgid weight/dry weight ratio (TW/DW) and water uptake capacity (WUC). When drought susceptibility index (DSI) among the genotypes was considered, BB24 was found the most tolerant to drought and BB04 was the most susceptible one. A close positive relationship between leaf TW/DW and DSI under drought was recorded (R ² = 0.627). Leaf TW/DW was decreased considerably due to water stress by 10% in genotype BB24 followed by BB43 (13%), and both BARI bushbean-2 and BB04 (19%). Stomatal aperture and whole plant transpiration rate were found minimal in the BB24 and BB43 compared to that of BB04 and BARI bushbean-2. Considering these water relation traits, genotypes BB24 and BB43 may be considered as relatively tolerant to tissue dehydration. The study also revealed that the TW/DW, WUC, stomatal aperture, and whole plant transpiration rate was negatively and significantly associated with yield; however, the RWC was positively correlated with yield under water stress conditions.  相似文献   

Molecular diversity and phenetic relationship of Momordica spp. of Indian occurrence     

L. K. Bharathi  S. K. Parida  A. D. Munshi  T. K. Behera  K. V. Raman  T. Mohapatra 《Genetic Resources and Crop Evolution》2012,59(5):937-948

Twenty-one RAPD and twelve ISSR primers were used for assessment of genetic diversity and establishing phenetic relationships among 35 genotypes of six currently cultivated Indian Momordica species and five genotypes of two Luffa species. A total of 436 RAPD and 230 ISSR scorable fragments were produced of which 99.8% fragments showed polymorphism among the species and varieties of Momordica and Luffa. The level of polymorphism detected by the 33 random primers was higher among the species (99.8%) of Momordica than that estimated among the varieties (61.3%). The varieties belonging to dioecious Momordica species (75.6%) showed a higher level of polymorphism as compared to monoecious species (50.3%). A significant level (68.6%) of polymorphism however was detected by the two marker types among the Indian varieties of monoecious M. charantia species. A wider range of molecular diversity (16–95%) detected by both RAPD and ISSR markers reflected presence of high level of genetic variation among the species and Indian varieties of Momordica and Luffa. The level of inter-specific diversity was maximum (90%) between annual monoecious M. charantia and perennial dioecious M. cochinchinensis whereas the extent of intra-specific diversity was highest particularly in dioecious species (51%) as compared to monoecious species like M. charantia (38%). Wider divergence of the taxon of controversial identity, M. cymbalaria from the other Indian cultivated Momordica species and their evolutionary closeness with Luffa species was evident. The clustering pattern obtained among the 40 genotypes belonging to different Momordica and Luffa species corresponded well with their morphological, cytological and taxonomic classification, which was further supported by high boot-strap values and PCA analysis. Species and genotype-specific fragments detected by the random markers would be useful in introgression breeding for genetic improvement of Momordica cultivated in India. A smaller set of 28 informative random markers screened in this study could precisely differentiate the Momordica genotypes from each other and thus would be of use in many marker-based genotyping applications in Momordica.  相似文献   

Isolation and pathotyping of H9N2 avian influenza viruses in Indian poultry     

Nagarajan S  Rajukumar K  Tosh C  Ramaswamy V  Purohit K  Saxena G  Behera P  Pattnaik B  Pradhan HK  Dubey SC 《Veterinary microbiology》2009,133(1-2):154-163

A total of 1246 faecal and tissue samples collected/received from 119 farms located in various states of India were processed for isolation of avian influenza viruses (AIV) during 2003-2004 as part of a program to monitor AIV infection in Indian poultry population. Avian influenza virus was isolated for the first time in India from poultry farms with history of drop in egg production, respiratory illness and increased mortality in Haryana state. A total of 29 H9N2 AIV isolates were obtained from the states of Punjab, Haryana, Uttar Pradesh, Gujarat, and Orissa and Union Territory Delhi. Subtyping was done by HI, RT-PCR and neuraminidase inhibition assay. Pathotyping of six representative isolates by intravenous pathogenicity index (0.0/3.0) in 6-8 weeks old chicken, trypsin dependency in cell culture and HA cleavage site analysis (335RSSR*GLF341) confirmed that these isolates are low pathogenic. Nucleotide sequence analysis of the HA gene showed that the Indian isolates are very closely related (95.0-99.6%) and shared a homology of 92-96% with H9N2 isolates from Germany and Asian regions other than that of mainland China. Deduced amino acid sequences showed the presence of L226 (234 in H9 numbering) which indicates a preference to binding of alpha (2-6) sialic acid receptors. Two of the six isolates had 7 glycosylation sites in the HA1 cleaved protein and the remaining four had 5 sites. Phylogenetic analysis showed that they share a common ancestor Qa/HK/G1/97 isolate which had contributed internal genes of H5N1 virus circulating in Vietnam. Further characterization of Indian H9N2 isolates is required to understand their nature and evolution.  相似文献