Genetic diversity of ash gourd [Benincasa hispida (Thunb.) Cogn.] inbred lines based on RAPD and ISSR markers and their hybrid performance     

Veerendra K. Verma  T.K. Behera  A.D. Munshi  Swarup K. Parida  T. Mohapatra 《Scientia Horticulturae》2007

Ten inbred lines of ash gourd [Benincasa hispida (Thunb.) Cogn.] were crossed to produce 45 F₁ hybrids (without reciprocal) which were evaluated along with the parents for 20 growth- and yield-related traits, in a replicated field trial. High level of heterosis was observed among the hybrids for most of the traits examined, including yield. These inbred lines were analysed by using 42 RAPD primers those produced 282 DNA marker bands. A total of 130 RAPD markers were obtained with a mean of 3.1 per primer, which in combination discriminated all the inbreds from each other. Pair-wise genetic distance measurements ranged from 0.07 to 0.31, suggesting a wide genetic diversity for these inbreds. These inberds were also analysed with five ISSR primers of which four were informative. Twenty-six ISSR marker bands were generated of which 11 were polymorphic with an average of 2.80 per primers. The percentage of polymorphic bands produced were higher in ISSR markers (>80%) than generated through RAPD markers (46%). Although the results indicated significant positive correlations of genetic distance with hybrid performance and heterosis, the RAPD based genetic distance measures and use of limited ISSR markers in this present study could not effectively predict hybrid performance in this crop. The genetic variation among ash gourd inbred lines examined, herein, defined a marker array (combined ISSR and RAPD) for the development of a standard reference for further genetic analyses, and the selection of potential parents for predicting hybrid performance and heterosis.  相似文献   

Yield and Zinc,Copper, Manganese and Iron Concentration in Maize (Zea mays L.) Grown on Vertisol as Influenced by Zinc Application from Various Zinc Fertilizers     

Sanjib K. Behera  Arvind K. Shukla  M. V. Singh  Ravi H. Wanjari  Pooja Singh 《Journal of plant nutrition》2015,38(10):1544-1557

Zinc (Zn) deficiency in soils and field crops is widespread across the world, including India, resulting in severe reduction in yield. Hence, soil application of Zn fertilizers is recommended for ameliorating Zn deficiency in soil and for obtaining higher crop yield and better crop quality. Zinc sulfate is commonly used Zn fertilizer in India because of its solubility and less cost. However, good quality and adequate quantity of zinc sulfate is not available in the market round the year for farmers' use. Field experiments were therefore conducted during rainy season of 2010 and 2011 at research farm of Indian Institute of Soil Science, Bhopal, India to assess the influence of Zn application through zinc sulfate monohydrate (33% Zn), zinc polyphosphate (21% Zn) and Zn ethylenediaminetetraacetate (EDTA) (12% Zn) on yield and micronutrient concentration and uptake by maize (Zea mays L.). In both the years, grain and vegetative tissue (stover) yield of maize increased significantly with successive application of Zn up to 1 kg ha^?1 added through zinc sulfate monohydrate and zinc polyphosphate. Addition of 2.5 kg Zn ha^?1 did not increase yield further but resulted in highest stover Zn concentration. Zinc, copper (Cu), manganese (Mn), and iron (Fe) concentration in maize grain varied from 22.2 to 27.6, 1.6 to 2.5, 3.5 to 4.7 and 19.9 to 24.5 mg kg^?1 respectively in both the years. Maize stover had 25.9 to 36.2, 7.9 to 9.8, 36.7 to 44.9 and 174 to 212 mg kg^?1 Zn, Cu, Mn, and Fe, respectively. Zinc application did not influence Cu, Mn and Fe concentration in both grain and stover of maize. Transfer coefficients (TCs) of micronutrients varied from 0.72 to 0.95, 0.18 to 0.30, 0.08 to 0.13 and 0.10 to 0.15 for Zn, Cu, Mn, and Fe respectively. Total Zn uptake significantly increased with Zn application from 0.5 to 2.5 kg ha^?1 supplied through zinc sulfate monohydrate and zinc polyphosphate. Recovery efficiency of Zn declined with increased Zn rates.  相似文献   

Cytomorphology of <Emphasis Type="Italic">Gentiana kurroo</Emphasis>: an important endangered bitter plant of temperate Himalaya     

M. C. Behera  R. Raina 《林业研究》2011,22(4):621-626

Gentiana kurroo, a potent bitter drug plant of Indian subcon- tinent, is under threat due to over exploitation and destruction of natural habitat. We studied the morphophenology and chromosomes of G. kur- roo on both wild and field grown plants, which is very important for proper identification, conservation and domestication. Results reveal that G. kurroo is a perennial herb, and its shoot is represented by flowering branches only. Stem is modified to rhizome. The older rhizomes split into four parts at collar region appearing to fuse together at the ends and is an important diagnostic feature for crude raw materials. Two types of leaves i.e. radical leaves at the base of the plant and cauline leaves on flowering shoot are present. Flowering occurs during September to Octo- ber with 1-9 inflorescences per plant. Inflorescence is terminal monoca- sial cysome type. Flowers are protrandus. Anthesis starts around 7.30 am and continued till 10.0 am. Ovary is bicarpillary syncarpous unilocular. Fruit is Capsule and takes 18 20 days to mature after fertilization. Seeds are very small elliptical and 1 000 seeds weigh to 0.1275 g. Chromoso- mal studies made by usual squash method reveals the species is a ge- nomic allotetraploid with n = 13. The anaphase-I segregation was normal and in none of the cells at Anaphase-I or Telophase-I could any abnor- mality like laggards, bridges, micronuclei etc. be observed.  相似文献   

Genetic Dissection of Grain Size Traits Through Genome-Wide Association Study Based on Genic Markers in Rice     

Amrit Kumar Nayak  Anilkumar C  Sasmita Behera  Rameswar Prasad Sah  Gera Roopa Lavanya  Awadhesh Kumar  Lambodar Behera  Muhammed Azharudheen Tp 《水稻科学》2022,29(5):462-472

Grain size plays a significant role in rice, starting from affecting yield to consumer preference, which is the driving force for deep investigation and improvement of grain size characters. Quantitative inheritance makes these traits complex to breed on account of several alleles contributing to the complete trait expression. We employed genome-wide association study in an association panel of 88 rice genotypes using 142 new candidate gene based SSR (cgSSR) markers, derived from yield-related candidate genes, with the efficient mixed-model association coupled mixed linear model for dissecting complete genetic control of grain size traits. A total of 10 significant associations were identified for four grain size-related characters (grain weight, grain length, grain width, and length-width ratio). Among the identified associations, seven marker trait associations explain more than 10% of the phenotypic variation, indicating major putative QTLs for respective traits. The allelic variations at genes OsBC1L4, SHO1 and OsD2 showed association between 1000-grain weight and grain width, 1000-grain weight and grain length, and grain width and length-width ratio, respectively. The cgSSR markers, associated with corresponding traits, can be utilized for direct allelic selection, while other significantly associated cgSSRs may be utilized for allelic accumulation in the breeding programs or grain size improvement. The new cgSSR markers associated with grain size related characters have a significant impact on practical plant breeding to increase the number of causative alleles for these traits through marker aided rice breeding programs.  相似文献   

Revealing Genetic Relationship and Prospecting of Novel Donors Among Upland Rice Genotypes Using qDTY-Linked SSR Markers     

Mitadru Mukherjee  Barada Padhy  Bharathkumar Srinivasan  Pradosh Mahadani  Sk Yasin Baksh  Ravindra Donde  Onkar Nath Singh  Lambodar Behera  Padmini Swain  Sushanta Kumar Dash 《水稻科学》2018,25(6):308-319

A total of 17 simple sequence repeat (SSR) markers linked to QTLs (qDTYs) governing grain yield under reproductive stage (RS) drought stress were used to assess the genetic relationship and prospecting new donors for qDTYs among 32 popular upland rice genotypes. These SSR markers generated a total of 36 alleles with an average allele count of 2.1 per locus. Polymorphic information content value of the markers ranged from 0.376 to 0.662 with an average value of 0.484. The expected heterozyogosity ranged from 0.381 to 0.632. STRUCTURE analysis divided the 32 genotypes into three sub-populations. Subsequent phenotyping revealed that all the tolerant genotypes were grouped into one sub-population, whereas the moderately tolerant and susceptible genotypes were grouped into separate sub-populations. Phylogenetic tree constructed by the unweighted neighbour-joining method also divided the genotypes into three clusters. The grouping pattern of genotypes into the clusters was similar to that into the STRUCTURE analysis, on the basis of drought tolerance level. The average value of genetic dissimilarity coefficient among the genotypes was observed to be 0.486. Furthermore, by combining genotyping data with phenotyping data, 16 new donors for 6 qDTYs were identified.  相似文献   

A Review on Heavy Metal Ions and Dye Adsorption from Water by Agricultural Solid Waste Adsorbents     

Sharmeen Afroze  Tushar Kanti Sen 《Water, air, and soil pollution》2018,229(7):225

Agricultural solid wastes either in natural or in modified forms have been successfully used for decades as non-conventional cost-effective adsorbents for removing metal ions and dyes from their aqueous phase and have been recognized as a sustainable solution for wastewater treatment. Therefore, this review article provides extensive literature information about heavy metals and dyes, their classifications and toxicity, various treatment methods with emphasis on adsorption characteristics by numerous agricultural solid wastes, or agricultural solid waste-derived adsorbents under various physicochemical process conditions. This review article not only provided an up-to-date information on the application of sustainable low-cost alternative adsorbents such as agricultural solid wastes, agricultural by-products, and biomass-based cost-effective activated carbon and various other natural materials in the batch adsorptive removal of heavy metal and dye from aqueous phase but also presented a comprehensive compilation of adsorptive pollutant removal information based on various reported continuous column operation studies which is one of the new aspect to this review article. The effectiveness of various batch and column operational process parameters on mechanistic adsorptive removal of both heavy metals and dyes by various agricultural solid waste-based adsorbents has been critically discussed here. Batch and column adsorption mechanism, batch kinetics, column dynamic modeling, and adsorptive behavior of adsorbents under various process parameters have also been critically analyzed and compared. Finally, literature information on recovery and regeneration through desorption techniques and cost comparison of various agricultural solid waste adsorbents with commercial activated carbons have also been reported here. Conclusions have been drawn from the literature reviewed, and few suggestions for future research direction are proposed.  相似文献   

Soil fertility and yield-limiting nutrients in oil palm plantations of north-eastern state Mizoram of India     

S. K. Behera  K. Suresh  B. N. Rao  K. Ramachandrudu  K. Manorama  P. Harinarayana 《Journal of plant nutrition》2017,40(8):1165-1171

A survey was conducted for assessment of soil fertility status, leaf nutrient concentration and finding yield-limiting nutrients of oil palm (Elaeis guineensis Jacq.) plantations in Mizoram state situated in the northeastern part of India. Soil pH, electrical conductivity (EC), organic carbon (OC), available potassium (K), available phosphorus (P) (Bray's-P), exchangeable calcium (Ca) (Exch. Ca) and magnesium (Mg) (Exch. Mg), available sulfur (S) (CaCl₂-S), and hot-water-soluble boron (B) (HWB) content in surface (0–20 cm depth) and subsurface (20–40 cm depth) soil layers varied widely. Diagnosis and Recommendation Integrated System (DRIS) norms were established for different nutrient expressions, and DRIS indices were computed. As per DRIS indices, the order of requirement of nutrients was found to be B > K > Mg > P > nitrogen (N). Optimum leaf nutrient ranges as per DRIS norms varied from 1.91% to 2.95%, 0.46% to 0.65%, 0.63% to 1.00%, 0.48% to 0.88%, and 9.41 to 31.0 mg kg^?1 for N, P, K, Mg, and B, respectively. On the basis of DRIS-derived optimum ranges, 32%, 9%, 27%, 12%, and 12% leaf samples had less than optimum concentration of N, P, K, Mg, and B, respectively. The optimum ranges developed could be used as a guide for routine diagnostic and advisory purpose for efficient fertilizer application.  相似文献   

Suitability of Lime,Green Leaf,and Farm Yard Manure and Inorganic Fertilizers for Sustaining Green Gram Yield and Rainwater Use Efficiency Under Moist Sub-Humid Alfisols in India     

Ashok Mishra  B. Behera  A. K. Pal  S. K. Mohanty  B. S. Rath 《Communications in Soil Science and Plant Analysis》2017,48(12):1417-1427

Field experiments were conducted to assess the effect of nutrients management practices on yield and rainwater use efficiency of green gram (Vigna radiata), and soil fertility under moist sub-humid Alfisols at Phulbani, India, during 2005–2008. Ten treatment combinations of lime @ 10% and 20% of lime requirement (LR) @ 8.3 t ha^?1, farmyard manure (FYM) @ 5 t ha^?1, green leaf manure @ 5 t ha^?1, and nitrogen, phosphorus, and potassium (N–P–K) (20–40–20 kg ha^?1) were tested. The analysis of variance indicated that treatments differed significantly from each other in influencing yield and rainwater use efficiency. Application of lime @ 20% LR + FYM @ 5 t/ha + 40 kg P + 20 kg K ha^?1 was superior with maximum mean yield of 531 kg ha^?1, while lime @ 10% LR + FYM @ 5 t ha^?1 + 40 kg P + 20 kg K ha^?1 was the second best with 405 kg ha^?1 and maintained maximum soil fertility of nutrients. The superior treatment gave maximum sustainability yield index of 67.5%, rainwater use efficiency of 0.49 kg ha^?1 mm^?1, improved soil pH, electrical conductivity, and soil nutrients over years.  相似文献   

Soil Carbon Sequestration in Long-Term Fertilization Under Jute-Rice-Wheat Agro-Ecosystem     

A. K. Singh  M. S. Behera  S. P. Mazumdar  D. K. Kundu 《Communications in Soil Science and Plant Analysis》2019,50(6):739-748

Soil organic carbon (SOC) sequestration in response to long-term fertilizer management practices under jute-rice-wheat agro-ecosystem in alluvial soils was studied using a modeling approach. Fertilizer management practices included nitrogen (N), phosphorus (P) and potassium (K) fertilization, manure application, and root-stubble retention of all three crops. Soil carbon (C) model RothC was used to simulate the critical C input rates needed to maintain initial soil C level in long timescale (44 years). SOC change was significantly influenced by the long-term fertilizer management practices and the edaphic variable of initial SOC content. The effects of fertilizer combination “100%NPK+FYM” on SOC changes were most significant over “100%NPK” fertilization. If the 100% NPK fertilizer along with manure applied with stubble and roots retention of all crops, alluvial soils of such agro-ecosystem would act as a net C sink, and the average SOC density kept increasing from 18.18 Mg ha^?1 during 1972 to the current average of ～22 Mg ha^?1 during 2065 s. On an average, the critical C input was estimated to be 5.30 Mg C ha^?1 yr^?1, depending on local soil and climatic conditions. The critical C input could be effectively estimated using a summary model driven by current SOC level, mean annual temperature, precipitation, and soil clay content. Such information will provide a baseline for assessing soil C dynamics under potential changes in fertilizer and crop residues management practices, and thus enable development of management strategies for effectively mitigating climate change through soil C sequestration.  相似文献   

Beta-glucan: an ideal immunostimulant in aquaculture (a review)   总被引：1，自引：1，他引：1  

D. K. Meena  Pronob Das  Shailesh Kumar  S. C. Mandal  A. K. Prusty  S. K. Singh  M. S. Akhtar  B. K. Behera  Kundan Kumar  A. K. Pal  S. C. Mukherjee 《Fish physiology and biochemistry》2013,39(3):431-457

The major hindrance in the development and sustainability of aquaculture industry is the occurrence of various diseases in the farming systems. Today, preventive and management measures are central concern to overcome such outbreak of diseases. Immunostimulants are considered as an effective tool for enhancing immune status of cultured organisms. Among different immunostimulants used in aquaculture practices, β-glucan is one of the promising immunostimulant, which is a homopolysaccharide of glucose molecule linked by the glycoside bond. It forms the major constituents of cell wall of some plants, fungi, bacteria, mushroom, yeast, and seaweeds. Major attention on β-glucan was captivated with the gain in knowledge on its receptors and the mechanism of action. The receptor present inside the animal body recognizes and binds to β-glucan, which in turn renders the animal with high resistance and enhanced immune response. This review highlights β-glucan as an immunostimulant, its effective dosages, and route of administration and furthermore provides an outline on role of β-glucan in enhancing growth, survival, and protection against infectious pathogens pertaining to fishes and shellfishes. Study also summarizes the effect of β-glucan on its receptors, recognition of proteins, immune-related enzymes, immune-related gene expression and their mechanisms of action.  相似文献