Soil fertility and yield-limiting nutrients in oil palm plantations of north-eastern state Mizoram of India     

S. K. Behera  K. Suresh  B. N. Rao  K. Ramachandrudu  K. Manorama  P. Harinarayana 《Journal of plant nutrition》2017,40(8):1165-1171

A survey was conducted for assessment of soil fertility status, leaf nutrient concentration and finding yield-limiting nutrients of oil palm (Elaeis guineensis Jacq.) plantations in Mizoram state situated in the northeastern part of India. Soil pH, electrical conductivity (EC), organic carbon (OC), available potassium (K), available phosphorus (P) (Bray's-P), exchangeable calcium (Ca) (Exch. Ca) and magnesium (Mg) (Exch. Mg), available sulfur (S) (CaCl₂-S), and hot-water-soluble boron (B) (HWB) content in surface (0–20 cm depth) and subsurface (20–40 cm depth) soil layers varied widely. Diagnosis and Recommendation Integrated System (DRIS) norms were established for different nutrient expressions, and DRIS indices were computed. As per DRIS indices, the order of requirement of nutrients was found to be B > K > Mg > P > nitrogen (N). Optimum leaf nutrient ranges as per DRIS norms varied from 1.91% to 2.95%, 0.46% to 0.65%, 0.63% to 1.00%, 0.48% to 0.88%, and 9.41 to 31.0 mg kg^?1 for N, P, K, Mg, and B, respectively. On the basis of DRIS-derived optimum ranges, 32%, 9%, 27%, 12%, and 12% leaf samples had less than optimum concentration of N, P, K, Mg, and B, respectively. The optimum ranges developed could be used as a guide for routine diagnostic and advisory purpose for efficient fertilizer application.  相似文献   

Assessment of genetic diversity and species relationships in eggplant (Solanum melongena L.) using STMS markers     

T.K. Behera  Pankaj Sharma  B.K. Singh  Gunjeet Kumar  Ravinder Kumar  T. Mohapatra  N.K. Singh 《Scientia Horticulturae》2006

Ninety six accessions including 92 of Solanum melongena and four related non-tuberous species (Solanum insanum, S. incanum, S. integrifolium and S. sysimbriifolium) were taken for the assessment of genetic diversity using 23 STMS primers. Eleven of the 23 primers tested showed polymorphism. The number of alleles per primer ranged from three to six with an average of 4.4. S. melongena had maximum average similarity with its closely related species, S. insanum (0.67) and minimum average similarity with the wild species, S. sysimbriifolium (0.50). The two weedy species S. incanum and S. integrifolium showed more average similarity value of 0.62 and 0.61, respectively with the cultivated S. melongena. S. insanum. S. incanum and S. integrifolium were relatively similar to each other with similarity index value of 0.61 (between S. insanum and S. incanum), 0.63 (between S. insanum and S. integrifolium) and 0.62 (between S. incanum and S. integrifolium). In contrast S. sysimbriifolium was most divergent with the similarity value of 0.49, 0.47 and 0.51 with S. insanum, S. incanum and S. integrifolium, respectively. The closely related species S. insanum and S. incanum, which clustered along with S. melongena accessions, being crossable with cultivated species, constitute important sources of genes that can be introgressed by backcross breeding. Molecular markers can be employed to identify the hybrids and also to monitor introgression of the useful genes.  相似文献   

Genetic diversity of ash gourd [Benincasa hispida (Thunb.) Cogn.] inbred lines based on RAPD and ISSR markers and their hybrid performance     

Veerendra K. Verma  T.K. Behera  A.D. Munshi  Swarup K. Parida  T. Mohapatra 《Scientia Horticulturae》2007

Ten inbred lines of ash gourd [Benincasa hispida (Thunb.) Cogn.] were crossed to produce 45 F₁ hybrids (without reciprocal) which were evaluated along with the parents for 20 growth- and yield-related traits, in a replicated field trial. High level of heterosis was observed among the hybrids for most of the traits examined, including yield. These inbred lines were analysed by using 42 RAPD primers those produced 282 DNA marker bands. A total of 130 RAPD markers were obtained with a mean of 3.1 per primer, which in combination discriminated all the inbreds from each other. Pair-wise genetic distance measurements ranged from 0.07 to 0.31, suggesting a wide genetic diversity for these inbreds. These inberds were also analysed with five ISSR primers of which four were informative. Twenty-six ISSR marker bands were generated of which 11 were polymorphic with an average of 2.80 per primers. The percentage of polymorphic bands produced were higher in ISSR markers (>80%) than generated through RAPD markers (46%). Although the results indicated significant positive correlations of genetic distance with hybrid performance and heterosis, the RAPD based genetic distance measures and use of limited ISSR markers in this present study could not effectively predict hybrid performance in this crop. The genetic variation among ash gourd inbred lines examined, herein, defined a marker array (combined ISSR and RAPD) for the development of a standard reference for further genetic analyses, and the selection of potential parents for predicting hybrid performance and heterosis.  相似文献   

Effects of high temperature on spikelet sterility in rice (Oryza sativa L.): association between molecular markers and allelic phenotypic effect in field condition     

Chidambaranathan  Parameswaran  Balasubramaniasai  Cayalvizhi  Behura  Niranjana  Purty  Mohini  Samantaray  Sanghamitra  Subudhi  Hatanath  Ngangkham  Umakanta  Devanna  B. N.  Katara  Jawahar Lal  Kumar  Awadhesh  Behera  Lambodar 《Genetic Resources and Crop Evolution》2021,68(5):1923-1935

Genetic Resources and Crop Evolution - Rice varieties are generally bred for higher yield but may possess genomic regions conferring tolerance to abiotic stresses. Climate change driven heat stress...  相似文献   

Genetic Dissection of Grain Size Traits Through Genome-Wide Association Study Based on Genic Markers in Rice     

Amrit Kumar Nayak  Anilkumar C  Sasmita Behera  Rameswar Prasad Sah  Gera Roopa Lavanya  Awadhesh Kumar  Lambodar Behera  Muhammed Azharudheen Tp 《水稻科学》2022,29(5):462-472

Grain size plays a significant role in rice, starting from affecting yield to consumer preference, which is the driving force for deep investigation and improvement of grain size characters. Quantitative inheritance makes these traits complex to breed on account of several alleles contributing to the complete trait expression. We employed genome-wide association study in an association panel of 88 rice genotypes using 142 new candidate gene based SSR (cgSSR) markers, derived from yield-related candidate genes, with the efficient mixed-model association coupled mixed linear model for dissecting complete genetic control of grain size traits. A total of 10 significant associations were identified for four grain size-related characters (grain weight, grain length, grain width, and length-width ratio). Among the identified associations, seven marker trait associations explain more than 10% of the phenotypic variation, indicating major putative QTLs for respective traits. The allelic variations at genes OsBC1L4, SHO1 and OsD2 showed association between 1000-grain weight and grain width, 1000-grain weight and grain length, and grain width and length-width ratio, respectively. The cgSSR markers, associated with corresponding traits, can be utilized for direct allelic selection, while other significantly associated cgSSRs may be utilized for allelic accumulation in the breeding programs or grain size improvement. The new cgSSR markers associated with grain size related characters have a significant impact on practical plant breeding to increase the number of causative alleles for these traits through marker aided rice breeding programs.  相似文献   

Molecular diversity and phenetic relationship of Momordica spp. of Indian occurrence     

L. K. Bharathi  S. K. Parida  A. D. Munshi  T. K. Behera  K. V. Raman  T. Mohapatra 《Genetic Resources and Crop Evolution》2012,59(5):937-948

Twenty-one RAPD and twelve ISSR primers were used for assessment of genetic diversity and establishing phenetic relationships among 35 genotypes of six currently cultivated Indian Momordica species and five genotypes of two Luffa species. A total of 436 RAPD and 230 ISSR scorable fragments were produced of which 99.8% fragments showed polymorphism among the species and varieties of Momordica and Luffa. The level of polymorphism detected by the 33 random primers was higher among the species (99.8%) of Momordica than that estimated among the varieties (61.3%). The varieties belonging to dioecious Momordica species (75.6%) showed a higher level of polymorphism as compared to monoecious species (50.3%). A significant level (68.6%) of polymorphism however was detected by the two marker types among the Indian varieties of monoecious M. charantia species. A wider range of molecular diversity (16–95%) detected by both RAPD and ISSR markers reflected presence of high level of genetic variation among the species and Indian varieties of Momordica and Luffa. The level of inter-specific diversity was maximum (90%) between annual monoecious M. charantia and perennial dioecious M. cochinchinensis whereas the extent of intra-specific diversity was highest particularly in dioecious species (51%) as compared to monoecious species like M. charantia (38%). Wider divergence of the taxon of controversial identity, M. cymbalaria from the other Indian cultivated Momordica species and their evolutionary closeness with Luffa species was evident. The clustering pattern obtained among the 40 genotypes belonging to different Momordica and Luffa species corresponded well with their morphological, cytological and taxonomic classification, which was further supported by high boot-strap values and PCA analysis. Species and genotype-specific fragments detected by the random markers would be useful in introgression breeding for genetic improvement of Momordica cultivated in India. A smaller set of 28 informative random markers screened in this study could precisely differentiate the Momordica genotypes from each other and thus would be of use in many marker-based genotyping applications in Momordica.  相似文献   

Isolation and pathotyping of H9N2 avian influenza viruses in Indian poultry     

Nagarajan S  Rajukumar K  Tosh C  Ramaswamy V  Purohit K  Saxena G  Behera P  Pattnaik B  Pradhan HK  Dubey SC 《Veterinary microbiology》2009,133(1-2):154-163

A total of 1246 faecal and tissue samples collected/received from 119 farms located in various states of India were processed for isolation of avian influenza viruses (AIV) during 2003-2004 as part of a program to monitor AIV infection in Indian poultry population. Avian influenza virus was isolated for the first time in India from poultry farms with history of drop in egg production, respiratory illness and increased mortality in Haryana state. A total of 29 H9N2 AIV isolates were obtained from the states of Punjab, Haryana, Uttar Pradesh, Gujarat, and Orissa and Union Territory Delhi. Subtyping was done by HI, RT-PCR and neuraminidase inhibition assay. Pathotyping of six representative isolates by intravenous pathogenicity index (0.0/3.0) in 6-8 weeks old chicken, trypsin dependency in cell culture and HA cleavage site analysis (335RSSR*GLF341) confirmed that these isolates are low pathogenic. Nucleotide sequence analysis of the HA gene showed that the Indian isolates are very closely related (95.0-99.6%) and shared a homology of 92-96% with H9N2 isolates from Germany and Asian regions other than that of mainland China. Deduced amino acid sequences showed the presence of L226 (234 in H9 numbering) which indicates a preference to binding of alpha (2-6) sialic acid receptors. Two of the six isolates had 7 glycosylation sites in the HA1 cleaved protein and the remaining four had 5 sites. Phylogenetic analysis showed that they share a common ancestor Qa/HK/G1/97 isolate which had contributed internal genes of H5N1 virus circulating in Vietnam. Further characterization of Indian H9N2 isolates is required to understand their nature and evolution.  相似文献   

Suitability of Lime,Green Leaf,and Farm Yard Manure and Inorganic Fertilizers for Sustaining Green Gram Yield and Rainwater Use Efficiency Under Moist Sub-Humid Alfisols in India     

Ashok Mishra  B. Behera  A. K. Pal  S. K. Mohanty  B. S. Rath 《Communications in Soil Science and Plant Analysis》2017,48(12):1417-1427

Field experiments were conducted to assess the effect of nutrients management practices on yield and rainwater use efficiency of green gram (Vigna radiata), and soil fertility under moist sub-humid Alfisols at Phulbani, India, during 2005–2008. Ten treatment combinations of lime @ 10% and 20% of lime requirement (LR) @ 8.3 t ha^?1, farmyard manure (FYM) @ 5 t ha^?1, green leaf manure @ 5 t ha^?1, and nitrogen, phosphorus, and potassium (N–P–K) (20–40–20 kg ha^?1) were tested. The analysis of variance indicated that treatments differed significantly from each other in influencing yield and rainwater use efficiency. Application of lime @ 20% LR + FYM @ 5 t/ha + 40 kg P + 20 kg K ha^?1 was superior with maximum mean yield of 531 kg ha^?1, while lime @ 10% LR + FYM @ 5 t ha^?1 + 40 kg P + 20 kg K ha^?1 was the second best with 405 kg ha^?1 and maintained maximum soil fertility of nutrients. The superior treatment gave maximum sustainability yield index of 67.5%, rainwater use efficiency of 0.49 kg ha^?1 mm^?1, improved soil pH, electrical conductivity, and soil nutrients over years.  相似文献   

Soil Carbon Sequestration in Long-Term Fertilization Under Jute-Rice-Wheat Agro-Ecosystem     

A. K. Singh  M. S. Behera  S. P. Mazumdar  D. K. Kundu 《Communications in Soil Science and Plant Analysis》2019,50(6):739-748

Soil organic carbon (SOC) sequestration in response to long-term fertilizer management practices under jute-rice-wheat agro-ecosystem in alluvial soils was studied using a modeling approach. Fertilizer management practices included nitrogen (N), phosphorus (P) and potassium (K) fertilization, manure application, and root-stubble retention of all three crops. Soil carbon (C) model RothC was used to simulate the critical C input rates needed to maintain initial soil C level in long timescale (44 years). SOC change was significantly influenced by the long-term fertilizer management practices and the edaphic variable of initial SOC content. The effects of fertilizer combination “100%NPK+FYM” on SOC changes were most significant over “100%NPK” fertilization. If the 100% NPK fertilizer along with manure applied with stubble and roots retention of all crops, alluvial soils of such agro-ecosystem would act as a net C sink, and the average SOC density kept increasing from 18.18 Mg ha^?1 during 1972 to the current average of ～22 Mg ha^?1 during 2065 s. On an average, the critical C input was estimated to be 5.30 Mg C ha^?1 yr^?1, depending on local soil and climatic conditions. The critical C input could be effectively estimated using a summary model driven by current SOC level, mean annual temperature, precipitation, and soil clay content. Such information will provide a baseline for assessing soil C dynamics under potential changes in fertilizer and crop residues management practices, and thus enable development of management strategies for effectively mitigating climate change through soil C sequestration.  相似文献   

Beta-glucan: an ideal immunostimulant in aquaculture (a review)   总被引：1，自引：1，他引：1  

D. K. Meena  Pronob Das  Shailesh Kumar  S. C. Mandal  A. K. Prusty  S. K. Singh  M. S. Akhtar  B. K. Behera  Kundan Kumar  A. K. Pal  S. C. Mukherjee 《Fish physiology and biochemistry》2013,39(3):431-457

The major hindrance in the development and sustainability of aquaculture industry is the occurrence of various diseases in the farming systems. Today, preventive and management measures are central concern to overcome such outbreak of diseases. Immunostimulants are considered as an effective tool for enhancing immune status of cultured organisms. Among different immunostimulants used in aquaculture practices, β-glucan is one of the promising immunostimulant, which is a homopolysaccharide of glucose molecule linked by the glycoside bond. It forms the major constituents of cell wall of some plants, fungi, bacteria, mushroom, yeast, and seaweeds. Major attention on β-glucan was captivated with the gain in knowledge on its receptors and the mechanism of action. The receptor present inside the animal body recognizes and binds to β-glucan, which in turn renders the animal with high resistance and enhanced immune response. This review highlights β-glucan as an immunostimulant, its effective dosages, and route of administration and furthermore provides an outline on role of β-glucan in enhancing growth, survival, and protection against infectious pathogens pertaining to fishes and shellfishes. Study also summarizes the effect of β-glucan on its receptors, recognition of proteins, immune-related enzymes, immune-related gene expression and their mechanisms of action.  相似文献