Cross-sectional study on acrylamide hemoglobin adducts in subpopulations from the European Prospective Investigation into Cancer and Nutrition (EPIC) Study     

Vesper HW  Slimani N  Hallmans G  Tjønneland A  Agudo A  Benetou V  Bingham S  Boeing H  Boutron-Ruault MC  Bueno-de-Mesquita HB  Chirlaque D  Clavel-Chapelon F  Crowe F  Drogan D  Ferrari P  Johansson I  Kaaks R  Linseisen J  Lund E  Manjer J  Mattiello A  Palli D  Peeters PH  Rinaldi S  Skeie G  Trichopoulou A  Vineis P  Wirfält E  Overvad K  Strömberg U 《Journal of agricultural and food chemistry》2008,56(15):6046-6053

Acrylamide exposure was investigated in subgroups of the EPIC study population (510 subjects from 9 European countries, randomly selected and stratified by age, gender, and smoking status) using hemoglobin adducts of acrylamide (HbAA) and its primary metabolite glycidamide (HbGA). Blood samples were analyzed for HbAA and HbGA by HPLC/MS/MS. Statistical models for HbAA and HbGA were developed including body mass index (BMI), educational level, and physical activity. A large variability in acrylamide exposure and metabolism between individuals and country groups was observed with HbAA and HbGA values ranging between 15-623 and 8-377 pmol/g of Hb, respectively. Both adducts differed significantly by country, sex, and smoking status. HbGA values were significantly lower in high alcohol consumers than in moderate consumers. With increasing BMI, HbGA in nonsmokers and HbAA in smokers decreased significantly. In the assessment of potential health effects related to acrylamide exposure, country of origin, BMI, alcohol consumption, sex, and smoking status should be considered.  相似文献   

Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus   总被引：1，自引：0，他引：1  

Nordengrahn A  Gustafsdottir SM  Ebert K  Reid SM  King DP  Ferris NP  Brocchi E  Grazioli S  Landegren U  Merza M 《Veterinary microbiology》2008,127(3-4):227-236

A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3 FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.  相似文献   

Metabolic profiles in five high-producing Swedish dairy herds with a history of abomasal displacement and ketosis     

Lena Steng?rde  Madeleine Tr?vén  Ulf Emanuelson  Kjell Holtenius  Jan Hultgren  Rauni Niskanen 《Acta veterinaria Scandinavica》2008,50(1):31

Background

Body condition score and blood profiles have been used to monitor management and herd health in dairy cows. The aim of this study was to examine BCS and extended metabolic profiles, reflecting both energy metabolism and liver status around calving in high-producing herds with a high incidence of abomasal displacement and ketosis and to evaluate if such profiles can be used at herd level to pinpoint specific herd problems.

Methods

Body condition score and metabolic profiles around calving in five high-producing herds with high incidences of abomasal displacement and ketosis were assessed using linear mixed models (94 cows, 326 examinations). Cows were examined and blood sampled every three weeks from four weeks ante partum (ap) to nine weeks postpartum (pp). Blood parameters studied were glucose, fructosamine, non-esterified fatty acids (NEFA), insulin, β-hydroxybutyrate, aspartate aminotransferase, glutamate dehydrogenase, haptoglobin and cholesterol.

Results

All herds had overconditioned dry cows that lost body condition substantially the first 4–6 weeks pp. Two herds had elevated levels of NEFA ap and three herds had elevated levels pp. One herd had low levels of insulin ap and low levels of cholesterol pp. Haptoglobin was detected pp in all herds and its usefulness is discussed.

Conclusion

NEFA was the parameter that most closely reflected the body condition losses while these losses were not seen in glucose and fructosamine levels. Insulin and cholesterol were potentially useful in herd profiles but need further investigation. Increased glutamate dehydrogenase suggested liver cell damage in all herds.  相似文献   

Therapeutic effects of systemic or intramammary antimicrobial treatment of bovine subclinical mastitis during lactation     

Sandgren CH  Waller KP  Emanuelson U 《Veterinary journal (London, England : 1997)》2008,175(1):108-117

The short- and long-term treatment efficacy of administrating penicillin for bovine subclinical mastitis during lactation when using intramuscular (IM; 9.5 mg [15,000 IU]/kg bodyweight of benzyl penicillin potassium) injections twice daily for 5 days, or intramammary (IMM; 0.3g [300,000 IU] penethamate hydroiodide) administration once daily for 5 days was compared with a control group receiving no treatment. One hundred and twenty-six cows met the inclusion criteria, which were lack of clinical symptoms, no recent treatment with antimicrobials, and findings of penicillin-sensitive Staphylococcus aureus, Streptococcus dysgalactiae, or Streptococcus uberis in combination with an inflammatory reaction. At follow-up 42-58 days after treatment, the proportion of cows negative for the original infection was significantly higher in IM and IMM groups compared to controls, but the difference between antimicrobial treatment groups was not significant. The udder quarter milk somatic cell count (SCC) was significantly lower at follow-up in IM and IMM groups than in controls, but milk production did not differ between treatments. The culling rate during the 10-month period following treatment was significantly higher in the group treated with IMM penicillin than in the other two groups, but the risk of new mastitis treatments within 10 months did not differ between the three groups. The cure rate was significantly affected by lactation number (lower in older cows), breed (lower in the Swedish Holstein breed), pathogen (lower for S. aureus), and pre-treatment SCC (higher for above average SCC). In conclusion, beneficial long-term effects of antimicrobial treatment during lactation of subclinical mastitis caused by S. aureus, Str. dysgalactiae or Str. uberis were not found in the present study.  相似文献   

A longitudinal study of seroprevalence and seroconversion of Neospora caninum infection in dairy cattle in northeast Thailand     

Chanlun A  Emanuelson U  Frössling J  Aiumlamai S  Björkman C 《Veterinary parasitology》2007,146(3-4):242-248

A long-term study was carried out in 11 dairy herds in the Khon Kaen province of northeast Thailand between August 2001 and November 2004. The objective was to investigate seroprevalence dynamics of Neospora caninum infection in the herds and to demonstrate patterns of seroconversion in individual cattle. Each herd was visited once a year, in total four times, and sera from cattle > 3 months of age and farm dogs as well as a sample from the bulk milk were collected. All samples were analysed for presence of specific antibodies by an N. caninum iscom ELISA. The overall percentage of antibody-positive cattle was constant and varied only between 10 and 13% over the 4 years, but the variation in within-herd seroprevalence between herds was substantial. Two herds had > or = 20% seropositive animals at all samplings and consistently high bulk milk OD, whereas two herds had no seropositive animal at the last two samplings and low bulk milk OD. Five herds had a decreasing trend of within-herd seroprevalence, whereas the remaining six herds had a higher portion of test-positive individuals at the end of the study. A total of 424 individuals were sampled more than once; 344 (81%) and 32 (8%) were consistently antibody-negative and antibody-positive, respectively. The proportions of animals that changed from being seronegative to seropositive and from being seropositive to seronegative between the years were 3.9-4.6% and 19-39%, respectively. Apparent vertical and horizontal transmission rates were 58% (95% CI; 44-71%) and 5% (95% CI; 3-7%), respectively. In conclusion, the overall percentage of N. caninum antibody-positive cattle was constant over the years, but the within-herd seroprevalence varied substantially between the herds. Seroconversions were likely to occur in individual cattle although most animals had consistent serological status throughout the study.  相似文献   

Effect of trawling with traditional and ‘T90’ trawl codends on fish size and on different quality parameters of cod <Emphasis Type="Italic">Gadus morhua</Emphasis> and haddock <Emphasis Type="Italic">Melanogrammus aeglefinus</Emphasis>     

Hanne Digre  Ulrik Jes Hansen  Ulf Erikson 《Fisheries Science》2010,76(4):549-559

The effect of trawling on fish size and on different quality parameters of cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) was evaluated after conducting 16 valid hauls using two trawls in a double rig fitted with a traditional and a novel ‘T90’ codend, respectively. The total catch volume during the fishing period was 47.6 metric tons, with an average catch per codend of 1.5 (range 0.5–2.9) tons. The mean haul duration was 5 h. The catch was assessed according to fish size, mortality, external damage, initial white muscle pH and development of rigor mortis. Fillet quality (colour, blood spots, gaping) was assessed after 1 week of freeze-storage. Our results showed there was no difference between the two types of nets in terms of catch volume, but significantly slightly bigger fish were caught with T90 than with the traditional trawl net (p < 0.05). Haddock caught with the traditional trawl net had more external injuries related to the trawl gear than haddock caught with the T90 gear (p < 0.05). The gaping frequency for cod caught with the traditional trawl net tended to be higher than cod caught with the T90 gear, but the difference was not significant (p = 0.07). No other differences in fish quality between fish caught in the trawl nets were observed.  相似文献   

Bleeding of anaesthetized and exhausted Atlantic salmon: body cavity inspection and residual blood in pre‐rigor and smoked fillets as determined by various analytical methods     

Ulf Erikson  Ekrem Misimi  Britta Fismen 《Aquaculture Research》2010,41(4):496-510

The bleeding efficiency of anaesthetized and exhausted Atlantic salmon was studied. Unbled fish were used as control groups for both treatments. Several analytical methods (computer vision, Minolta Chroma Meter, transflectance spectroscopy, haemoglobin iron and visual assessment of smoked fillets) were used to evaluate colour or residual blood contents of pre‐rigor and smoked fillets. In all cases, the amount of residual blood in the fillets was modest and blood was not considered a quality problem in terms of fillet appearance. Perimortem stress did not affect residual blood contents of pre‐rigor fillets. Only salting and smoking had a significant effect on filet colour. The low levels of residual blood was partly attributed to filleting shortly after killing allowing washing before the blood had time to coagulate. In addition, a computer vision method was developed for automated blood inspection of the body cavity after gutting and washing. A classifier (‘no blood’ or ‘blood present’) based on linear discriminant analysis was tested and the classification accuracy was over 90% as evaluated with the leave‐one‐out method.  相似文献   

Research into fluorinated pyrethroid alcohols—an episode in the history of pyrethroid discovery     

Klaus Naumann 《Pest management science》1998,52(1):3-20

An account of pyrethroid research from 1975 to 1985 at Bayer AG is given. The exploitation of fluorine chemistry for this purpose led to increased activity of known 3-phenoxybenzyl pyrethroid esters and to the commercialisation of the broad-spectrum insecticide cyfluthrin, the particularly tick-toxic flumethrin and the rapid-acting household insecticides fenfluthrin and transfluthrin. The last two constituted in 1976 a novel type of pyrethroid, based on polyfluorinated benzyl alcohols, off the mainstream of published pyrethroid research. Transfluthrin, the single isomer (1R)trans-permethric acid ester of 2,3,5,6-tetrafluorobenzyl alcohol has just been introduced to the market. The history of its discovery and structure–activity data as well as resistance considerations regarding cyfluthrin, are presented. © 1998 SCI.  相似文献   

The alkaloid Ageladine A, originally isolated from marine sponges, used for pH-sensitive imaging of transparent marine animals     

Bickmeyer U 《Marine drugs》2012,10(1):223-233

The brominated pyrrole-imidazole Ageladine A was used for live imaging of the jellyfish (jellies) Nausithoe werneri, the sea anemone Metridium senile and the flatworm Macrostomum lignano. The fluorescence properties of Ageladine A allow for estimation of pH values in tissue and organs in living animals. The results showed that Nausithoe werneri had the most acidic areas in the tentacles and close to the mouth (pH 4-6.5), Metridium senile harbours aggregates of high acidity in the tentacles (pH 5) and in Macrostomum lignano, the rhabdoids, the gonads and areas close to the mouth were the most acidic with values down to pH 5.  相似文献   

Inner Alpine conifer response to 20th century drought swings     

Pascale Affolter  Ulf Büntgen  Jan Esper  Andreas Rigling  Pascale Weber  Jürg Luterbacher  David Frank 《European Journal of Forest Research》2010,129(3):289-298

Tree rings from the Alpine area have been widely used to reconstruct variations in summer temperature. In contrast, estimates of changes in the hydrological cycle are rather scarce. In order to detect altitudinal and species-specific patterns of growth responses to anomalous dry and wet conditions, a large network of 53 tree-ring width site chronologies along the Rhone valley (Valais, Switzerland) covering the 1751–2005 period was compiled and analyzed. A total of 1,605 measurement series from four conifer species—pine, larch, spruce, fir—were detrended to allow inter-annual to multi-decadal scale variability to be preserved. Site chronologies were combined to four altitudinal (colline: <800 m asl, sub-montane: 800–1,000 m asl, montane: 1,000–1,450 m asl, sub-alpine: >1,450 m asl) and species-specific mean time-series. These records were compared with temperature, precipitation, and drought (scPDSI) data. Among the altitudinal records, the colline chronologies showed highest correlation with June precipitation and scPDSI (0.5 and 0.7). Altitudinal effects, via control on climatic conditions, were superimposed upon species-specific characteristics in affecting tree growth and response to moisture variations. In particular, species-specific differences affected the significance level of drought response, with decreasing drought sensitivity towards higher elevations. Growth conditions were found to be optimal at ~1,300 m asl, with precipitation/drought limiting tree growth below and temperature above. Common years of extreme drought and low growth for the colline sites occurred in 1921, 1944, 1976, 1992, and 1998. Our results demonstrate the potential of lower elevation conifers for reconstructing long-term changes in Alpine hydro-climate.  相似文献