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41.
Andaluz A Moll X Abellán R Ventura R Carbó M Fresno L García F 《Veterinary journal (London, England : 1997)》2009,181(3):299-304
The purpose of this study was to evaluate plasma concentrations and pharmacokinetic parameters of buprenorphine in dogs following intravenous (IV) administration of clinical doses of the opioid. An IV bolus of 0.02 mg/kg buprenorphine was administered to six healthy Beagles and blood samples were collected through a jugular catheter before and at 1, 5, 10, 15, 20, 30 and 45 min, and 1, 2, 4, 6, 8 and 12 h after administration. Plasma buprenorphine concentrations, measured using a commercial radioimmunoassay (RIA), decreased following a three-exponential curve. The two distribution and the elimination half-lives were 2.9 ± 1.8 min, 16.5 ± 3.7 min, and 266.6 ± 82.0 min, respectively; the clearance was 329.6 ± 62.2 mL/min, and the steady state volume of distribution was 83.7 ± 26.5 L.The results demonstrated the feasibility of the RIA assay to analyse buprenorphine in dog plasma samples. Following IV administration buprenorphine showed a three-compartment kinetic profile, as has been described previously in humans, rabbits and cats. The relationship between plasma concentrations and dynamic effects in dogs remains to be established. 相似文献
42.
Zingiberene-mediated resistance to the South American tomato pinworm derived from Lycopersicon hirsutum var. hirsutum 总被引:1,自引:0,他引:1
Sebastião Márcio de Azevedo Marcos Ventura Faria Wilson Roberto Maluf Ana Cláudia Barneche de Oliveira Joelson André de Freitas 《Euphytica》2003,134(3):347-351
The Lycopersicon hirsutum var. hirsutum accession PI 127826 is recognized as a good source of resistance to arthropod pests due to the action of the allelochemical
zimgiberene, a sesquiterpene present in its glandular trichomes. Five genotypes were selected from the F2 generation of the interspecific cross Lycopersicon esculentum ‘TOM-556’ × Lycopersicon hirsutum var. hirsutum ‘PI 127826’, based on their low levels (BPX-368-clone#56) or high levels(BPX-368-clone#92, BPX-368-clone#105,BPX-368-clone#179,
BPX-368-clone#250) of zingiberene. The five F2 genotypes were tested for resistance to the South American tomato pinworm Tuta absolutaalong with accession L. esculentum ‘TOM-556’ (pinworm susceptible), and the accessions L. hirsutum var. hirsutum ‘PI 127826’ and L. pennellii ‘LA716’ (resistant). The F2 clones selected for high foliar zingiberene levels showed lower scores for leaflet lesion type(LLT), percent leaflets attacked
(PLA) and overall plant damage (OPD) than the low zingiberene genotypes. The results indicated that zingiberene mediates resistance
to the South American pinworm, based on feeding and on ovipositing deterrence, in populations derived from the interspecific
cross between Lycopersicon. esculentum and Lycopersicon hirsutum var. hirsutum. Indirect selection for high foliar zingiberene content is suggested as an efficient technique for breeding tomatoes for
resistance to the South American tomato pinworm.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
43.
B. Companioni N. Mora L. Díaz A. Pérez M. Arzola P. Espinosa M. Hernández J. Ventura M. C. Pérez R. Santos J. C. Lorenzo 《Plant Breeding》2005,124(1):79-85
Among the most important crops in developing countries are banana and plantain. However, the production is threatened by increasingly virulent forms of Fusarium wilt, and therefore, intensive breeding programmes are being carried out worldwide. As conventional field studies of banana resistance to this disease are time‐consuming and destructive, an easy‐to‐do procedure was previously developed to differentiate field‐grown resistant and susceptible banana cultivars at leaf level. Such a procedure involved the in vitro treatment of fungal culture filtrates on to field‐grown adult leaves and the measurement of lesion areas 48 h later. The present report includes measurements of other indicators such as biochemical compounds. The cultivar ‘Gross Michel’ (susceptible) and cv. ‘FHIA‐01’ (resistant) leaves were treated with Fusarium oxysporum f. sp. cubense race 1 culture filtrates. Evaluations were performed 48 h after leaf treatment. Compared with culture medium‐treated leaves (control treatment), fungal metabolites produced leaf lesions, decreased freephenolic contents and increased protein levels in both cultivars. In ‘FHIA‐01’, the culture filtrate increased contents of cell wall‐linked phenolics and the pool of aldehydes (except malondialdehyde). Fungal metabolites did not cause variations in peroxidase activity, chlorophyll pigment contents or malondialdehyde level in any cultivar. The use of Fisher's linear discriminant analysis to differentiate resistant and susceptible banana cultivars in breeding programmes is also a novel aspect of this report. Such an estimation was performed from a data matrix that included the effects of the fungal metabolites (leaf lesion area and levels of free and cell wall‐linked phenolics, aldehydes, except malondialdehyde, and proteins) on banana leaves of seven cultivars (four susceptible and three resistant). 相似文献
44.
45.
The feeding preference of the collembolan Protaphorura armata in the presence of Fusarium culmorum and Gaeumannomyces graminis var. tritici, two soil-borne fungi pathogenic for winter cereals, was studied in a simplified experimental system including wheat seedlings. Analysis of gut content of all animals from microcosms containing inoculum of both fungi showed that F. culmorum was clearly preferred but that G. graminis var. tritici was also fed. At microscopic examination the majority of F. culmorum conidia present in the gut lacked cytoplasmic content, and only few conidial cells were intact. The feeding preference of P. armata favoured G. graminis var. tritici over F. culmorum in the competition for infection sites on wheat plants; in fact, the former resulted the prevalent cause of plant disease.
The viability of fungal propagules after passage through the gut of P. armata was also studied. No colonies of G. graminis var. tritici and only a few colonies of F. culmorum developed from faecal pellets set on agar medium. Fungal propagules dispersed by springtails were not sufficient to induce disease, as demonstrated by introducing animals, previously fed on fungal cultures separately, into microcosms containing a sterile substrate where wheat kernels were seeded. 相似文献
46.
The cocoa roasting process at different temperatures (at 125 and 135 degrees C for 3 min, plus 44 and 52 min, respectively, heating-up times) was evaluated by measuring the initial and final free amino acids distribution, flavor index, formol number, browning measurement, and alkylpyrazines content in 15 cocoa bean samples of different origins. These samples were also analyzed in manufactured cocoa powder. The effect of alkalinization of cocoa was studied. Results indicated that the final concentration and ratio of tetramethylpyrazine/trimethylpyrazine (TMP/TrMP) increased rapidly at higher roasting temperatures. The samples roasted with alkalies (pH between 7.20 and 7.92), such as sodium carbonate, or potassium plus air injected in the roaster during thermal treatment, exhibited a greater degree of brown color formation, but the amount of alkylpyrazines generated was adversely affected. The analysis of alpha-free amino acids at the end of the roasting process demonstrated the importance of the thermal treatment conditions and the pH values on nibs (cocoa bean cotyledons), liquor, or cocoa. Higher pH values led to a lower concentration of aroma and a higher presence of brown compounds. 相似文献
47.
48.
Thomas Larsen Marc Ventura Diane M. O'Brien Bente Aa. Lomstein 《Soil biology & biochemistry》2011,43(4):749-759
Soil animal detritivores play an important role in facilitating decomposition processes but little information is available on how the quality of dietary resources affects their stoichiometry of carbon (C) nitrogen (N) and phosphorus (P), and turnover of C and N. This study investigated how a fungal diet, Fusarium culmorum, with a low N content and imbalanced amino acid (AA) composition affected the physiology of three soil-dwelling collembolans (Folsomia candida, Protaphorura fimata and Proisotoma minuta) in comparison to a control diet, Saccharomyces cerevisiae, with a high N content and balanced AA composition. We compared the elemental composition of animals, their growth rates and tissue replacement of C and N. We also measured the individual AA δ13C to investigate the extent that Collembola may rely on endogenous sources to compensate for scarcity of essential AAs. The results showed that animal's N content tracked closely the composition of their diets, decreasing from around 10 to 7% N from the high to low N diet. They also had a significant increase of C and a decrease of P. P. fimata was less affected than F. candida and P. minuta. The total incorporation of C and N in the animals due to growth and tissue replacement decreased from 11-17 to 6-12% DM d−1 on the high and low N diet respectively with P. fimata experiencing the smallest change. Essential AAs δ13C did not always match perfectly between Collembola species and their diets; particularly on the low N diet. Isotope patterns of AAs indicate that bacteria may have been the alternative source of essential AAs. While the results of this study cannot be extrapolated directly to the dynamics of Collembola populations in the field, they serve to demonstrate their flexibility in adapting physiologically to the temporal and spatial patchiness of the soil environment. 相似文献
49.
Detection of two viral genomes in single cells by double-label hybridization in situ and color microradioautography 总被引:5,自引:0,他引:5
A T Haase D Walker L Stowring P Ventura A Geballe H Blum M Brahic R Goldberg K O'Brien 《Science (New York, N.Y.)》1985,227(4683):189-192
Double labeling and color microradioautography were used in a new method of hybridization in situ to identify different genes in individual cells. The method is based on the unequal penetration of 3H and 35S into two layers of nuclear track emulsion separated by a thin barrier film. Hybridization of a 35S-labeled probe specific for one kind of gene results in silver grains over cells in both layers of emulsion; a 3H-labeled probe for a second gene provides grains only in the first layer of emulsion. Silver grains are converted to magenta-colored grains in the first layer and to cyan-colored grains in the second to facilitate enumeration of grains in each layer. This technique should be widely applicable in analyses of differential gene expression in single cells or in discrete populations of cells. 相似文献
50.
Ester Abranches Costa Portela Jorge Ventura Ferreira-Cardoso José Luis Louzada 《Journal of plant nutrition》2013,36(9):1245-1253
Although boron (B) deficiency in chestnuts (Castanea sativa Mill.) has been identified in Portugal and B fertilization was carried out in some orchards, the post-treatment evaluations have not been made to date. So the objective of this trial was to confirm the B deficiency and to quantify the effect of B applications to the soil on nut yield and quality. In October 2006, a fertilizer trial was established in a 15 year-old orchard in a very acid soil derived from siliceous schists. Soil liming and a basal fertilization were carried out in 16 trees and two levels of sodium tetraborate (Granubor, 14.6% B) were applied to eight trees: control (B0) and 100 g of Granubor per tree (B1). In the beginning of September chestnut leaves were collected from five trees and analyzed for macro- and micronutrients. Nut productivity was measured per tree and some nut quality parameters were evaluated, including the chemical composition of the kernel (soluble sugars, starch, total fiber, crude protein, and crude fat) in 2007, and dry matter, nut caliber, and fruit damage in 2007 and 2008. Boron fertilization of chestnuts significantly increased nut production: 75% in the first year (8 kg and 14 kg per tree, respectively, in the B0 and B1) and, in the second year, was over four times higher (4 kg and 17 kg per tree, respectively, in the B0 and B1). No significant differences were found in dry matter, nut caliber, nut damage and chemical composition of the kernel in the first year, but in the second year lower fruit damage by chestnut tortrix (P<0.0089) was observed. Foliar analyses exhibit relatively low B concentrations in the control trees (average value of 8 mg kg?1) in both years, while in the fertilized trees the foliar B concentration were in average 61 mg kg?1 in the first year, and 34 mg kg?1 in the second year. The large drop in the foliar B concentration in the second year suggests the need to monitoring the nutrient status of chestnut orchards or more frequent of B application than usual practice. 相似文献