Association analysis for detecting significant single nucleotide polymorphisms for phosphorus-deficiency tolerance at the seedling stage in soybean [Glycine max (L) Merr.]     

Lihua Ning  Guizhen Kan  Wenkai Du  Shiwei Guo  Qing Wang  Guozheng Zhang  Hao Cheng  Deyue Yu 《Breeding Science》2016,66(2):191-203

Tolerance to low-phosphorus soil is a desirable trait in soybean cultivars. Previous quantitative trait locus (QTL) studies for phosphorus-deficiency tolerance were mainly derived from bi-parental segregating populations and few reports from natural population. The objective of this study was to detect QTLs that regulate phosphorus-deficiency tolerance in soybean using association mapping approach. Phosphorus-deficiency tolerance was evaluated according to five traits (plant shoot height, shoot dry weight, phosphorus concentration, phosphorus acquisition efficiency and use efficiency) comprising a conditional phenotype at the seedling stage. Association mapping of the conditional phenotype detected 19 SNPs including 13 SNPs that were significantly associated with the five traits across two years. A novel cluster of SNPs, including three SNPs that consistently showed significant effects over two years, that associated with more than one trait was detected on chromosome 3. All favorable alleles, which were determined based on the mean of conditional phenotypic values of each trait over the two years, could be pyramided into one cultivar through parental cross combination. The best three cross combinations were predicted with the aim of simultaneously improving phosphorus acquisition efficiency and use efficiency. These results will provide a thorough understanding of the genetic basis of phosphorus deficiency tolerance in soybean.  相似文献   

天水106份冬小麦品种（系）Yr18基因和1BL/1RS易位的分子检测     

王娜  刘鸿燕  周喜旺  张耀辉  岳维云  宋建荣  魏志平  安勤生  曹世勤 《甘肃农业科技》2019,(8):27-32

选用与Yr18基因和1BL/1RS易位系紧密连锁的SCAR和STS标记,对天水106份供试冬小麦育成品种(系)Yr18基因、1BL/1RS易位系的分布情况进行分子检测。结果表明,106份材料中,有2份材料检测到Yr18基因,有55份材料检测到1BL/1RS易位系,分别占被检测材料的1.89%、51.89%。对目前流行条锈菌小种有良好抗性且表现慢锈性的Yr18基因在甘肃天水小麦育种中的利用率较低,而1BL/1RS易位系的利用率仍然较高。品系15-16H307和07-144-2-1-1-1同时检测到1BL/1RS易位系和Yr18基因,建议在甘肃天水小麦抗条锈病育种中合理应用。  相似文献   

常利率通货膨胀下有扰动的双复合Poisson-Geometric过程的两险种风险模型     

魏广华  高启兵  刘国祥 《金陵科技学院学报》2015,(2)

考虑常利率及通货膨胀下有扰动的双复合Poisson-Geometric过程的两险种风险模型,运用鞅方法得到破产概率满足的Lundberg不等式和一般公式,当保费和索赔服从指数分布和混合指数分布时,得到破产概率的精确表达式。  相似文献   

非洲猪瘟疫情背景下兽医公共卫生学课程的教学策略     

李郁  孙裴  徐前明  刘雪兰  魏建忠 《畜牧与饲料科学》2019,40(10):102-104

兽医公共卫生学是利用一切与人类和动物健康问题有关的理论知识、实践活动和物资资源,为人类健康事业服务的一门综合性应用学科,在动物性食品安全、动物疫病及人兽共患病方面发挥着重要的防线作用。针对当前我国发生的非洲猪瘟疫情,在阐述兽医公共卫生学重要性的基础上,分析了兽医公共卫生学课程的教学策略,以期打破对兽医专业认识的局限性,强化理解兽医公共卫生的意义,提升兽医公共卫生学的课程价值,并为优化教学内容、提高教学质量提供指导。  相似文献   

长梗润楠扦插生根对IBA、NAA和IAA的响应   总被引：2，自引：0，他引：2  

孙昂  李莲芳  郭樑  苏柠 《北方园艺》2017,(16)

长梗润楠(Machilus longipedicellata)是西南地区的特有树种之一,也是优良的绿化树种。以长梗润楠为试材,采用U_7(7~6)方法,研究了IBA、NAA、IAA对长梗润楠扦插生根的影响。结果表明:扦插9个月时,平均愈伤组织产生率、生根率、一级根数、一级根长和一级根直径分别为78.9%~85.6%、84.5%~92.2%、2.7~3.7条·株~-1、9.7~14.6cm和1.22~1.58mm;0.75g·L~(-1) IAA+0.4g·L~(-1) IBA组合的生根率、平均一级根数、一级根直径和根长分别达85.5%、3.5条·株-1、1.58mm和13.5cm,为生根性状综合指标较优的处理组合。穗条的生根率与愈伤组织产生率间呈极显著正相关(P≈0.0000.01),二者的相关模型为Y=26.846+0.629 X(R2=0.506,R2Adj=0.484)。  相似文献   

Sequence Analysis and Prokaryotic Expression of NSP1 Gene of Bovine Rotavirus UK Strain     

RAN Xu-hua  CAO Si  ZHANG Yao  WEI Xiao-man  WEN Xiao-bo  NI Hong-bo  ZHU Zhan-bo 《中国畜牧兽医》2015,42(5):1104-1109

Rotavirus is a major cause of acute diarrhea in both many kinds of young animals and children under 5 years old.Rotavirus NSP1, a 55 ku RNA binding protein, is the product of gene 5, which can subvert innate immune responses and be one of virulent determinant factors.According to the sequence in GenBank, specific primers targeting to NSP1 gene were designed and the gene was amplified by RT-PCR, following by being cloned into the pET-28a(+) vector.It showed that the full length of NSP1 gene was 1 473 bp, encoding 491 amino acids.The NSP1 shared the highest identity with WC3 strain.The recombinant protein was induced in E.coli Rosetta(DE3) by IPTG and was analyzed by SDS-PAGE and Western blotting.The results revealed that NSP1 recombinant protein existed in the form of inclusion body with the molecular weight of 55 ku.The purified recombinant protein could be recognized by His-tag antibody.This study laid the foundation for further research on the relationship between the intracytoplasmic location of NSP1 protein and its activity.  相似文献   

创新方法在农业领域科研项目实施过程中的应用     

黄倩  金鑫  张燕鹏  胥伟  王斌 《现代农业科技》2020,(17)

本文基于创新方法在我国农业领域科研项目中的应用现状,总结概括了其在实施过程中不同的创新特征,以“七大作物育种为例”分析了创新方法应用过程中存在的问题及相关建议,为创新方法在科研项目实施过程中发挥驱动作用提供参考,提高科研创新效率。  相似文献   

玉米植株数量性状配合力及其利用价值分析     

胡军  魏开锋  刘万  郭鹏程  徐风雨  潘洪玉  张鑫生 《黑龙江农业科学》2020,(1):1-8

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巴马县超级稻纹枯病的发生特点与防控对策     

莫桂康  ;韦袭芹 《现代农业科技》2014,(14):118-119

超级稻是巴马县近年来主推的重要水稻品系之一。由于其生长旺盛,在生育中后期长势浓郁,稻秆基部易遭受纹枯病的侵染危害。对该县甲篆乡等乡镇超级稻纹枯病的发生进行调查,分析其发病的主要原因,并提出有效的防治对策,以供稻田生产参考。  相似文献   

Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice     

LIU Lin  #  ZHANG Ying xin  #  LI Zhi  LIU Qun en  YU Ning  SUN Bin  YANG Zheng fu  ZHOU Quan  CHENG Shi hua  CAO Li yong 《中国水稻科学》2014,28(5):465

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