昆虫抗真菌肽研究的科学意义及其应用前景     

杨婉莹  黄亚东 《广东蚕业》2001,35(4):45-47

昆虫抗真菌是近年来刚刚起步的一个新的研究方向，国外目前已发现6种昆虫抗真菌肽因子。国内华南农业大学蚕学分子生物学与生物技术实验室课题组在此方向也取得一些重要进展，初步发现13种昆虫可能含有新的昆虫抗真菌肽基因。对昆虫抗真菌肽及其基因的研究，将大大拓宽昆虫抗菌肽的生物技术应用前景。  相似文献   

武威地区天然草地有毒植物及其防除   总被引：12，自引：2，他引：10  

万国栋  胡发成 《草业科学》1996,13(1):4-7

对武威地区天然草地有毒植物的种类，分布及其对草地的危害情况进行了调查研究，结果表明，全区天然草地共有毒植物１７２号，分属３１科８４属，平均２．１５种／ｍ＾２，盖度达４．４１％地上部分生物量１１．２８ｇ／ｍ＾２。对草地危害的主要黄花棘豆，甘肃棘豆，小花棘豆，狼毒和马草，受侵害的草地主要是山地草甸和山地草原，通过分析，提出了有毒植物防除和利用的建议。  相似文献   

The loss of genetic diversity in Dabry's sturgeon (Acipenser dabryanus,Dumeril) as revealed by DNA fingerprinting     

Qiu‐Hong Wan  Sheng‐Guo Fang  Ya‐Nan Li 《水产资源保护:海洋与淡水生态系统》2003,13(3):225-231

• 1. Dabry's sturgeon, a large, long‐lived migratory fish is endemic to the Yangtze River. Over‐fishing and habitat destruction have caused large‐scale declines in natural stocks in the last two decades.
• 2. Examining patterns of genetic diversity has become an integral component of many management plans for endangered species. DNA fingerprinting was applied to detect genetic diversity in Dabry's sturgeon collected in 1958–1959, 1980–1981 and 1998–1999.
• 3. Studies on direct genetic parameters (genetic variability, hypervariable loci and heterozygosity) and indirect parameters (band‐sharing coefficient and allelic frequency) showed that the continuous decline in wild populations has caused the loss of genetic diversity in present‐day sturgeon.
• 4. The present‐day populations have the lowest genetic variability; thus, effective management is needed to preserve genetic diversity.
• 5. A conservation strategy is urgently required, comprising artificial rearing facilities coupled with breeding management plans.

Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

Immunogenicity of bovine parainfluenza type 3 virus proteins encapsulated in nanoparticle vaccines,following intranasal administration to mice   总被引：2，自引：0，他引：2  

Shephard MJ  Todd D  Adair BM  Po AL  Mackie DP  Scott EM 《Research in veterinary science》2003,74(2):187-190

Stimulation of long lasting, protective immunity to respiratory viruses is often difficult to achieve with conventional respiratory vaccines. Polymeric nanoparticles, incorporating viral proteins have been shown to offer sustained release of antigen, with consequent prolongued stimulation of the respiratory immune system. In this paper the efficacy of two nanoparticle vaccines (poly-lactide-co-glycolide, PLGA; polymethylmethacrylate, PMMA), incorporating proteins of bovine parainfluenza type 3 virus (BPI-3) was investigated. As a preliminary to experiments in calves, it was considered essential to demonstrate immunogenicity of the experimental vaccine in mice. Mice immunised with PLGA nanoparticles, containing BPI-3 proteins, developed higher levels of virus-specific antibody than mice immunised with the PMMA vaccine or with soluble viral proteins alone. Immunoblotting using serum from the vaccinated mice, demonstrated strong reactions against the major BPI-3 proteins.  相似文献   

Characterization of monoclonal antibodies against avian reovirus S1133 protein sigmaA synthesized in Escherichia coli     

Pai WC  Shien JH  Liu HJ  Lee LH 《Veterinary microbiology》2003,91(4):309-323

Monoclonal antibodies (MAbs) were prepared against avian reovirus S1133 protein sigmaA (esigmaA) synthesized in Escherichia coli. MAbs were characterized and used to develop a diagnostic test. Ten MAbs were selected for competitive binding assay following coupling with horseradish peroxidase. The results indicated that these MAbs delineated two epitopes I and II of esigmaA. An immuno-dot binding assay was used to detect the effect of denaturation on antibody recognition of the epitopes. All MAbs bound to esigmaA in its native form. After denaturation by boiling in SDS and 2-mercaptoethanol, the binding of MAbs recognizing epitope I was fully abolished. However, the reactivity of MAbs recognizing epitope II was not affected. MAbs 31 and 32, recognizing epitopes I and II, respectively, were selected for the cross-reactivity to heterologous reovirus strains. The results suggest that the two epitopes are highly conserved among these virus strains. A MAb capture enzyme-linked immunosorbent assay (ELISA) procedure was developed using MAbs 32 and 31 to detect reovirus protein sigmaA in samples from tendon tissues of infected bird and chicken embryo fibroblast (CEF) cell cultures. Avian reovirus sigmaA antigens in tendon specimens were detected from the inoculated birds as early as 2 days post-inoculation (PI), approximated a peak at 7 days PI, and maintained this until 16 days PI, then decreased gradually. A clear difference in absorbance values between the tendon samples of the avian reovirus- and mock-infected birds is obtained. Positive results were also obtained from avian reovirus-infected CEF and from the tendon tissues of naturally infected broilers. These results indicated that the MAb capture ELISA is a useful methods for the detection of avian reovirus from chickens suspected to have avian reovirus infections.  相似文献   

溶菌酶在食品工业中的应用   总被引：2，自引：0，他引：2  

谷绒  车振明  万国福 《乳业科学与技术》2006,28(6):264-266

本文综述了溶菌酶的基本性质，着重讨论了溶菌酶抑制的微生物种类、机理，系统的阐述了溶菌酶的制备和在食品工业生产过程中应用的进展情况。  相似文献   

双重排水条件下控制措施对银南灌区水稻田水盐关系的影响分析   总被引：4，自引：0，他引：4  

贾忠华  罗纨  方树星  景卫华  WANG Liang 《干旱区资源与环境》2006,20(5):213-216

针对宁夏银南灌区目前存在的排水过量问题,研究者提出了利用控制排水措施,在适当时段减少排水量的建议。然而,灌区在农沟以及更深层的支、干沟及黄河(低地)的影响下,受着深层排水和浅层排水的双重作用。其中深层排水一般不会受到田间措施的影响,它的存在“淡化或中和”了由于浅层排水的减少引起的田间含盐量的增加。本文在对双重排水情况下的水盐运动进行概化的基础上,建立了基于特定地区排水特性的“浅层排水与深层排水比-浅层排水控制率-排水含盐量增加率”的关系。最后结合根据地下水洗淋的临界情况,确定了达到这一目标控制指标以及浅层地下水控制率。结果显示,对于研究的典型情况,当地下水的盐分含量达到临界的1.62 g/l时,可以将1m深的农沟控制至30cm深;这时的浅层地下水仅为现状的14%,即从731mm减少到了102mm。  相似文献   

鸭副黏病毒的分离与鉴定   总被引：1，自引：0，他引：1  

李建军  丁巧玲  袁生  袁圣丹  万碧燕  朱晓玲 《中国家禽》2006,28(24):135-136,139

从广东省某鸭场的病死番鸭肝脏中分离一株病毒,该病毒对雏鸭、种鸭、蛋鸭均可引起发病。接种鸭胚传至第6代时病毒对鸡红细胞的血凝性稳定,通常在72h开始致死非免疫鸭胚,死亡率为80%。经磷钨酸负染电镜观察,病毒粒子多呈现椭圆形,长轴为167～264nm,短轴为131～139nm,病毒外表有囊膜,囊膜外层有排列整齐的纤突。病毒与禽流感病毒无抗原相关性,能被禽副黏病毒I型阳性血清所中和。根据试验结果,初步将分离的病毒判定为鸭副黏病毒。  相似文献   

Guided bone regeneration with beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone membrane in partial defects of canine humerus     

Taehoon Oh  Md. Mizanur Rahman  Ji-Hey Lim  Mi-Sun Park  Dae-Yong Kim  Jung-hee Yoon  Wan Hee Kim  Masanori Kikuchi  Junzo Tanaka  Yoshihisa Koyama  Oh-Kyeong Kweon 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(1):73-77

This study was performed to evaluate the effect of beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone (TCP/PLGC) membrane in the repair of partial bone defects in canine proximal humerus. Three adult mixed-breed dogs were used during the experimental period. The length of the defect was quarter of the full length of humerus, and width of the defect was quarter of middle diameter of the lateral aspect of humerus. The humeri of each dog were divided into treatment (TCP/PLGC) and control groups. The defect was covered with TCP/PLGC membrane in treatment group. To evaluate regeneration of the bone, computerized tomography (CT) and histopathologic examination were performed. The radiopaque lines were appeared at the original defect sites in TCP/PLGC group but below the original site in control at 4th week. Radiopacity and thickness of the defect sites, and radiopaque lines were more increased at 8th week than those of 4th week. Histopathologic findings revealed fibrous connective tissue migration into the defect and the migration inhibited the structure of new cortex to be placed in the original level in control whereas new cortex growth was found in the level of original line in TCP/PLGC group. However, the new cortical bone in the TCP/PLGC group was thinner and less organized than the adjacent intact cortex, and the amount of new cancellous bones were also scanty. The result suggested that TCP/PLGC membrane is a good guided bone regeneration material to restore the original morphology of humerus in partial defect.  相似文献   

水杨酸与蔬菜作物抗性关系的研究进展   总被引：4，自引：0，他引：4  

万正林  罗庆熙 《西南园艺》2006,34(3):30-32

水杨酸在植物的抗病性方面发挥着重要的作用,近年来的研究表明,水杨酸在植物抗冷、热、盐、干旱、臭氧、重金属等环境胁迫方面有明显的作用。对近年来有关水杨酸在蔬菜抗逆境胁迫方面的研究进展进行综述,并提出一些研究思路。  相似文献