侵染西瓜的小西葫芦花叶病毒的生物学特性鉴定   总被引：1，自引：0，他引：1  

张大伟  范卫红  王杰  李世访  成卓敏 《植物保护学报》2004,31(4):437-438

西瓜病毒病是制约西瓜产量及品质的顽疾.据报道,该病害造成的经济损失达到30%～50%.1981～1999年皖、苏、鲁、豫西瓜的病毒病曾几次大发生.危害西瓜的病毒病有西瓜花叶病毒1号(WMV-1)、西瓜花叶病毒2号(WMV-2)、黄瓜花叶病毒(CMV)、烟草花叶病毒(TMV)、烟草环斑病毒(TRSV)、南瓜花叶病毒(SqMV)等32种[1].小西葫芦花叶病毒(ZYMV)是侵染葫芦科的主要病毒之一,1973年首次在意大利北部发现此病毒,至今已成为一个全球性的植物病毒[2].笔者对ZYMV合肥分离物的生物学特性进行了研究.  相似文献   

玉米叶片受新月弯孢菌侵染后的细胞病理学变化   总被引：6，自引：0，他引：6  

黄丽丽  王兰  康振生  赵杰  张管曲 《植物病理学报》2004,34(1):21-26

 本文利用透射电子显微镜技术与细胞化学技术研究了玉米叶片受弯孢菌侵染后的超微结构和细胞壁的组成成份变化。透射电镜观察发现,病菌侵入后,菌丝先在寄主细胞间扩展,随着寄主细胞病变、坏死,菌丝可进入寄主细胞形成胞内菌丝。随病菌侵入和在寄主体内扩展,寄主细胞先后发生了一系列的超微结构变化,叶绿体、液泡等细胞器解体,出现质壁分离现象,并最终解体、坏死、变形。细胞化学标记定位发现,受侵寄主细胞壁中纤维素、木聚糖和果胶质的标记密度明显低于未接种的健康组织,表明细胞壁降解酶(如纤维素酶、木聚糖酶和果胶酶)的产生与病菌侵染和致病过程密切相关。  相似文献   

基于GIS的祁连山森林景观格局分析   总被引：24，自引：2，他引：24  

杨国靖  肖笃宁  赵成章 《干旱区研究》2004,21(1):27-32

在地理信息系统软件ArcGIS环境下 ,将祁连山区DEM图和坡向图分别同植被图叠加 ,分析研究区各景观组分在空间的分布特征 ;用定量分析景观结构和景观格局程序Fragstats计算景观和各景观组分的相关指数 ,分析其连通性、完整性、破碎化程度及聚集程度。结果表明 :研究区各景观组分分布受海拔高度和坡向的影响非常明显。各景观组分的完整性、连通性和破碎化程度也很不均衡。草地是研究区面积最大、连通性和完整性最好的景观组分 ;青海云杉林呈斑块状或带状分布在阴坡和半阴坡 ,形状最为不规则 ,平均斑块面积小且距离近 ,易受干扰而发生重大变化 ;宜林地和祁连圆柏林相对于农田、疏林地有较强的扩张特征 ;而杨类阔叶林各斑块间相隔距离大 ,斑块之间的邻接性差 ,破碎化程度最为严重。  相似文献   

Model establishment and biological behaviour observation of mouse blastocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metastatic potential in vitro     

LI Da-qiang  WANG Zhi-biao  BAI Jin  ZHAO Jie  HU Kai  WANG Yuan  DU Yong-hong 《园艺学报》2004,20(1):32-37

AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P＜0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P＞0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life.  相似文献   

Regulation of MMP-2 and TIMP-3 expression by uPA signal transduction system in human bone giant cell tumor     

XU Ruo-bing  WEN Jian-ming  ZHANG Meng  LV Chang-hai  XIAO Gang  ZHANG Wen-min  LIANG Hui-zhen 《园艺学报》2004,20(11):1982-1988

AIM: To study effects of urokinase-type plasminogen activator (uPA) signal transduction on expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in giant cell tumor of bone (GCT). METHODS: Expression of uPAR, MMP-2 and TIMP-3 in GCT tissue was detected by immunohistochemistry. Phosphorylation level of mitogen-activated protein kinase (p44) in uPA/uPAR signal pathway in cultured GCT cells was detected by immunoprecipitation. The expression of MMP-2 and TIMP-3 in cultured cells after treatment with uPA-ATF or anti-uPAR antibody was also detected by Western blotting. RESULTS: 1) Urokinase-type plasminogen activator receptor (uPAR) was positive on the cell membrane and in cytoplasm of some mononuclear stromal cells (MSCs) and multinucleated giant cells (MGCs); 2) MMP-2 was positive in the cytoplasm and on the cell membrane of almost all of MSCs and some of MGCs. The polar distribution of MMP-2 in the cytoplasm of MGCs was especially obvious; 3) The expression of TIMP-3 of some MSCs and MGCs in GCT was much lower than MMP-2. The positive signal also showed a prominent polarity; 4) After treatment with uPA-ATF, the phosphorylation level of p44 in GCT cultured cells was much higher than the control. Addition of anti-uPAR antibody in the cells remarkably down-regulated the phosphorylation level of p44 as compared with the control group, suggesting that uPA-ATF participates cell signal transduction and this reaction can be inhibited by anti-uPAR antibody; 5) uPA-ATF cell signal pathway up-regulated expression of MMP-2 and TIMP-3, while anti-uPAR antibody down-regulated the expression of MMP-2 and TIMP-3. CONCLUSION: These results demonstrate for the first time that uPA-ATF directly regulates the expression of MMP-2 and TIMP-3 by signal transduction pathway, and the over-expression of MMP-2 and TIMP-3 may play an important role in local osteolysis of GCT.  相似文献   

Relationship of p21^WAF1 gene polymorphisms with protein expression in breast carcinomas     

XIAO Ning-xin  LI Zhong-hong  JIN Yi  WANG Guo-qiang  SU Zu-lan 《园艺学报》2004,20(6):976-980

AIM: To investigate the relationship between p21^WAF1gene polymorphisms and protein expression in breast carcinoma. METHODS: Polymerase chain reaction single-strand conformation polymorphisms technique (PCR-SSCP) and immunohistochemical assay of S-P immunostaining technique were used to study polymorphisms of p21^WAF1 and protein expression respectively on the specimen of paraffin-embedded tissues in 100 cases of breast carcinomas and 40 benign breast diseases as control. RESULTS: Two p21^WAF1 gene polymorphisms were found in 18% (18/100) of breast carcinomas and 5% (2/40) of control samples. The difference between the two groups was statistically significant (χ²=3.94, P＜0.05). The positive immunohistochemical reaction of p21^WAF1 protein were found in 50% (50/100) of breast carcinomas and 12.5% (5/40) of control samples. The difference between the two groups was statistically significant (χ²=16.84, P＜0.01). The positive immunohistochemical reaction of p21^WAF1 protein were found in 100% (18/18) of breast carcinomas with p21^WAF1 gene polymorphisms and 39% (32/82) of no p21^WAF1 gene polymorphisms. The difference between two groups was statistically significant (χ²=21.95, P＜0.01). The p21^WAF1 gene polymorphisms were correlated with the protein expression in breast carcinomas (r=0.576, P＜0.01). CONCLUSION: p21^WAF1 gene polymorphisms may create the different copies of mRNA and may make relevant protein molecules.  相似文献   

Induction of tumor-specific cytotoxic T lymphocytes in vitro by dendritic cells pulsed with different glioma antigens     

ZHU Xin-mei  QIAO Jian  XIAO Bao-guo  LU Chuan-zhen 《园艺学报》2004,20(4):584-589

AIM: The goal of this study was to compare different methods for tumor antigen preparation, to observe the induction of tumor-specific cytotoxic T lymphocytes in rats by dendritic cells (DCs) pulsed with different tumor antigens. METHODS: The precursors of dendritic cells were isolated from bone marrow of rats, stimulated in vitro with recombinent rat granulocyte-macrophage colony-stimulating factor (rrGM-CSF) and interleukin-4 (rrIL-4). Then rat DCs were pulsed with C6 tumor cell antigens prepared with different methods: freeze-thaw, boiling or total protein extracted from ultrasonic crushed tumor cell. Subsequently primed DCs were cocultured with T lymphocytes isolated from spleen to induce CTL. Lymphocyte chemoattractant factor from DCs and cytokine IFN-γ release were determined by ELISA, the cytotoxicity of CTL was assayed by JAM test. RESULTS: DCs pulsed with boiled tumor cell in vitro induced an enhanced ability of T-cell proliferation and cytotoxic T lymphocyte activity.CONCLUSION: Our results demonstrated that DCs primed with boiled tumor cell may represent a method for inducing immune responses against the entire repertoire of tumor antigens of malignancies.  相似文献   

禽流感病毒夹心ELISA快速检测方法的研究   总被引：21，自引：1，他引：21  

肖运才  李自力  胡思顺  石德时  许青荣  程峰  刘梅  毕丁仁  王桂枝 《畜牧兽医学报》2004,35(5):536-541

以禽流感病毒(AIV)湖北分离株(H9N2亚型)提纯物为免疫原，制备出鸡抗AIV和兔抗AIV抗体，经用琼脂扩散法测得效价分别为1:32和1:16。以纯化的鸡抗AIVIgG为包被抗体，兔抗AIVIgG为第二抗体，建立了检测AIV抗原的夹心ELISA法。结果表明，鸡抗AIVIgG的最佳包被浓度为1μg/mL，兔抗AIV IgG的最适工作浓度为5μg/mL；对已知的阳性样品，用夹心ELISA法测得的病毒滴度比血球凝集滴度高16倍以上，且能检出其它亚型的禽流感病毒；与新城疫病毒、传染性支气管炎病毒、减蛋综合征病毒、传染性喉气管炎病毒、传染性法氏囊病病毒、鸡痘病毒、马立克氏病病毒等无交叉反应，说明本方法有很高的特异性及敏感性。对14个鸡场送检的、患有呼吸道疾病或有腹膜炎、眼炎、产蛋下降、怀疑为禽流感感染的病鸡进行了检测，结果有7个鸡场为阳性。本方法的建立为禽流感病鸡群的临床检验提供了一种方便、敏感、快速的检测方法。  相似文献   

利用土壤筛选紫花苜蓿高效共生根瘤菌的初步研究   总被引：5，自引：0，他引：5  

刘杰  王赟文  李颖  汤辉  汪玲玲 《草业科学》2005,22(2):21-25

采用西北地区土壤试管栽培法对12株不同地区来源的根瘤茵和2个紫花苜蓿Medicago sativa品种(品系)进行接种效果的研究，旨在使筛选结果尽量接近实际生产环境，筛选出符合实际效果的高效共生菌株。结果表明，紫花苜蓿与根瘤菌之间表现出共生效果的多样性。绝大多数接种根瘤菌的紫花苜蓿在总瘤数、有效瘤数、地上部干质量方面与不接种的对照相比分别增加了7％～240％，10％～367％，7％～150％。根瘤菌与紫花苜蓿品种之间存在着互作关系，且受土壤因子尤其是土壤养分状况的影响。初步筛选出的与2个紫花苜蓿品种(品系)最佳共生匹配的根瘤菌菌株分别是：中苜一号的73317，01006，01055；金皇后的83092，01006，01055。  相似文献   

密花香薷挥发油成分的分析研究   总被引：9，自引：0，他引：9  

张继  王振恒  姚健  黄爱仑  谷丽萍  王杰  郭香玲 《草业学报》2005,14(1):112-116

采用水蒸气蒸馏法和气相色谱-质谱-计算机联用法对密花香薷的挥发性成分进行了分析和鉴定.分离出93个峰,确认了其中的84种化合物,其含量占全油的88.34%.主要化学成分为大根香叶烯(18.83%);D-柠檬烯(11.17%);2,5,5-三四基-1,3,6-庚三烯(6.30%);6-亚甲基-双环[3,1,0]己烷(5.90%);氧化石竹烯(3.94%);石竹烯(3.36%);4-碳-3,5-二甲基环己-1-烯(2.88%);α-3-环己烯-1-醇(2.06%).  相似文献