城市化与“三农”问题及云南的路径选择     

彭尔瑞  王穗  张川  张建生  杨志雷 《广东农业科学》2010,37(9):267-268,280

通过阐述城市化和"三农"问题的特点以及城市化对"三农"的影响进行分析探讨,并结合云南"三农"发展现状提出了云南城市化是解决"三农"问题的重要途径,提出了建设五级核心城市带动辐射周边的城市化发展思路和重点加大农村小城镇建设对促进云南农业、农村、农民发展的建议。  相似文献   

火炬松芽苗移栽容器育苗技术     

侯娜  陈骏  杨春华  张金国 《贵州林业科技》2012,(4):50-52

介绍了火炬松形态特征及生物学特性,并论述了其种子处理、苗床、育苗基质的准备,播种、芽苗移栽、幼苗的管理以及病虫害防治等技术措施。  相似文献   

绿壳蛋鸡IGF-Ⅰ基因SNPs分析及其与末期产蛋性能的相关性研究     

崔梓扬  杨洪雁  张宜强  白秀娟 《中国畜牧兽医》2012,39(12):125-128

本研究以胰岛素样生长因子Ⅰ(insulin-like growth factors-Ⅰ, IGF-Ⅰ)基因作为候选基因,运用PCR-RFLP法检测基因变异,并分析其变异与鸡末期产蛋性能的相关性。以绿壳蛋鸡(n=113)作为试验材料,对IGF-Ⅰ基因5'调控区进行遗传多样性研究。研究结果表明,该调控区扩增产物经PstⅠ酶切后出现AA、AB、BB共3种基因型,经χ²检验绿壳蛋鸡在IGF-Ⅰ位点上符合Hardy-Weinberg平衡状态(P＞0.05)。相关分析表明,IGF-Ⅰ基因5'调控区PstⅠ位点对于产蛋末期(14和15月龄)的产蛋性能没有显著影响(P＞0.05)。  相似文献   

Effects of High Fluoride and Low Iodine on Thyroid Function in Offspring Rats     

GE Ya-ming  NING Hong-mei  GU Xin-li  YIN Mei  YANG Xue-feng  QI Yong-hua  WANG Jun-dong 《中国农业科学(英文版)》2013,(3):502-508

Thirty-two Wistar rats were divided randomly into four groups of eight with six females and two males in each group. The rats were exposed to high fluoride drinking water (45 mg F- L-1 from 100 mg NaF L-1), low dietary iodine (0.0855 mg kg-1), or both together in order to assess the effects of these three regimens on the thyroid function of the offspring rats. After the animal model was established, the offspring rats were bred and 10-, 20-, 30-, 60-, and 90-d-old rats were used for the experiment. The treatments for the offspring rats were the same as those of their parents. In comparison with control rats, the relative thyroid glands were changed by three regimens, but the mean values of thyroid weight in the experimental groups saw no marked difference. Serum TT3 levels were increased in all stages in the low iodine (LI) group. In the high fluoride (HiF) group, increase in TT3 levels was observed except in 20-d-old rats. Decrease in TT3 at 20- and 90-d and increase in TT3 at 30- and 60-d were found in HiF+LI group. Serum TT4 levels first saw an increase, and then dropped in the LI and HiF+LI group. However, an increase in TT4 was found in the HiF group. The levels of TSH in serum rocketed at d 20, and then dropped in the next stages in experimental groups. The results suggested that thyroid disorder could be induced by high flroride in drinking water, low iodine diet, or both of them. Exposure time to fluoride or low iodine diet was one of the important factors that fluoride can induce the development of thyroid dyfunction.  相似文献   

Screening and Analysis of Proteins Interacting with TaPDK from Physiological Male Sterility Induced by CHA in Wheat   总被引：1，自引：0，他引：1  

ZHANG Long-yu  ZHANG Gai-sheng  ZHAO Xin-liang  YANG Shu-ling 《中国农业科学(英文版)》2013,(6):941-950

To further research the regulatory network of pyruvate dehydrogenase kinase (designated as TaPDK) in physiological male-sterility (PHYMS) of wheat induced by chemical hybridizing agent (CHA) SQ-1, an anther cDNA library was constructed, and the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR. Yeast-two hybrid screening was performed by mating yeast strain Y2HGold containing BD-TaPDK bait plasmid with yeast strain Y187 including anther cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium (SD/-Ade/-His/-Leu/-Trp) (QDO), and further were incubated on QDO medium containing AbA and X-α-Gal. The interactions between TaPDK and the proteins obtained from positive colonies were further confirmed by co-transformation validation. After plasmids DNA were extracted from blue colonies and sequenced, the sequences results were analyzed by bioinformatic methods. Finally, 24 colonies were obtained, including eight genes, namely non-specific lipid-transfer protein precursor (TanLTP), polyubiquitin (TaPUbi), glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen (TaPCNA), CBS domain containing protein (TaCBS), actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and three new genes with unknown function. The results of quantitative RT-PCR showed that the expression levels of TanLTP, TaPUbi, and TaPCNA were obviously up-regulated in PHYMS anther, and TaCBS expression was only increased at the tricellular stage in PHYMS anther compared with in fertile lines. Whereas, the expression of TaPDK was obviously down-regulated in PHYMS lines. Collectively, these datas indicated that the majority of candidate proteins might be related to pollen abortion in PHYMS lines, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.  相似文献   

四川短尾鼩不同体重个体分布及种群繁殖力变化     

龙贵兴  杨再学  何永贵  彭忠勇 《山地农业生物学报》2013,32(4)

对贵州省大方县1996 ～2012年采集的134只四川短尾鼩体重进行统计分析,结果表明:四川短尾鼩体重最低为12.20 g,最高达48.00 g,平均体重为(31.12±6.48)g,其雌、雄个体体重分布均呈常态分布,个体体重主要分布在22.1 ～40.0 g之间,占总兽类数的85.07％,是活动能力较强的个体.四川短尾鼩达到性成熟的体重界线为22.0 g,怀孕个体最低体重为26.00 g,睾丸下降个体最低体重为23.80 g,随着个体的增长,体重的增加,种群繁殖力不断增强,体重达30.0 g以上时,种群繁殖力迅速增加,平均怀孕率、睾丸下降率和繁殖指数分别为43.75％、80.95％和1.53,说明体重达30.0 g以上个体大部分已达到性成熟,是参与繁殖的主要个体.  相似文献   

基于移动端作物大田测试数据采集技术研究与实现     

王虎  杨耀华  李绍明  刘哲  许哲  张晓东  朱德海 《中国农业科技导报》2013,15(4):156-162

作物品种田间测试是评价、筛选新品种不可或缺的关键环节，是品种大规模推广应用的主要依据。育种单位每年试验小区数量巨大，单一育种公司每年新产生大约1 000万个数据点，依靠传统的人工纸质录入效率低、错误率高。针对测试数据田间野外录入的特点，研究设计了一套高效的测试数据快速录入、及时传输的技术方案，并详细介绍了客户端与服务器数据交互融合、拍照采集条码、数据分块存取等关键技术。该系统进行了Windows Mobile 6.1及以上多个手机平台的应用测试，结果表明此方法是高效可行的。  相似文献   

基于组态王的玉米果穗烘干监控系统设计     

张大龙  赵武云  杨婉霞  蒋五洋 《中国农业科技导报》2013,15(2):115-119

为充分保证种子烘干质量和发芽率，避免种子烘干效率低、人为操作失误等问题，采用研华ADAM4000系列数据采集模块，设计了基于组态王6.53的玉米果穗烘干监控系统，实现了对影响玉米果穗烘干质量的温度、压力数据的采集、处理和监控。在工控机上使用组态王实现了对数据分析处理、故障报警等功能，为提高果穗烘干质量提供了技术保障。应用情况表明，系统运行稳定可靠，人机界面友好，能够满足监控系统实时性、可靠性的要求。  相似文献   

基于SRAP的西瓜种质资源遗传多样性评价     

李朋飞  霍秀爱  程永强  代亮  杨炳艳  段会军 《中国农业科技导报》2013,15(2):89-96

运用SRAP分子标记技术分析了80份西瓜种质资源的遗传多样性，28对不同的引物组合共扩增出308条谱带，其中多态性谱带68条。引物组合的多态性频率最低为9.1%，最高为45.5%，平均22.1%。基于全部材料的SRAP基因型数据评价品种间的遗传关系，80份西瓜种质的遗传相似系数变化范围在0.94～1.00之间，表明供试西瓜种质资源的遗传背景比较狭窄。利用NTSYS软件聚类分析，80份西瓜材料被划分为3个类群，类群间相似度较高。来源地不同的西瓜材料也被划分到一个类群，进一步说明供试材料遗传背景狭窄。西瓜育种要取得突破性进展，必须加强种质资源的引进与创新。  相似文献   

中国门窗的发展     

杨冰冰  宋魁彦* 《林业机械与木工设备》2013,(1):19-21

介绍了中国古代门窗和现代门窗的发展历程,阐明了各时期门窗的特点及发展状况;探讨了古代门窗形制的变化特点、现代门窗用材和制作工艺的发展历程。  相似文献