西葫芦新品种济葫2号的选育     

李秀启  卢立轩  陈坤  马朝喜  刘星明  黄莎莎 《长江蔬菜》2005,(4):48-49

济葫2号是以QL0133和QLO119配制而成的西葫芦一代杂交种,植株生长旺盛、株型紧凑、矮蔓、早熟、丰产,抗病毒病、白粉病、蔓枯病.瓜浅绿色,嫩瓜长20.0 cm,粗6.0 cm,粗细均匀、品质佳.每667 m2产量达6 500kg,适于早春茬、秋冬茬及越冬茬栽培.  相似文献   

从甜瓜子叶中提取总DNA   总被引：4，自引：1，他引：4  

陆璐  赵长增  陶兴林 《果树学报》2005,22(6):748-750

用分子标记方法检测甜瓜杂交种子纯度,如果从真叶中提取DNA,检测周期至少需要15d。为了缩短检测周期,我们分别以甜瓜的成熟干种子、吸胀水后“露白”种子、3d龄刚转绿子叶、6d龄子叶、9d龄子叶和12d龄子叶为材料,进行了提取总DNA的研究。结果表明:除了干种子和吸胀水后“露白”种子外,其他的材料都可以提取到DNA,但是DNA的质量因子叶日龄不同而存在很大的差异。不同日龄的子叶中,以3d龄子叶提取的DNA质量好。子叶6d龄时,提取的DNA质量次于3d龄,少量DNA开始出现降解,以后随着子叶日龄的增加,DNA降解加重。另外,与以真叶为材料提取的DNA样品相比较,子叶DNA样品中的蛋白质等杂质含量高,应增加氯仿抽提纯化次数。  相似文献   

青脚鸡杂交组合肉用性能的研究     

窦套存  王克华  陆俊贤  童海兵 《中国家禽》2005,9(Z1):31-33

以江苏省家禽科学研究所家禽育种中心崇仁麻鸡♂×(崇仁麻鸡♂×隐性白羽鸡新品系♀)♀(配套1)和固始鸡♂×(崇仁麻鸡♂×隐性白羽鸡新品系♀)♀(配套2)为素材,进行肉用性能试验,结果表明①两配套组合体重差异极显著(P＜0.01),配套2比配套1重109.6克且饲料报酬高0.171;②两配套组合龙骨长、冠长、冠高、胸宽差异不显著(P＞0.05),体斜长、胫长、胫围、胸角差异极显著(P＜0.01),配套2均高于配套1.③两配套组合屠体率、半净膛率、全净膛率、胸肌率、腹脂率差异不显著(P＞0.05),配套1腿肌率显著高于配套2(P＜0.05).④两配套组合胸肌失水率、PH值、肉色、剪切力差异不显著(P＞0.05).总体而言,配套2的肉用性能优于配套1,隐性白羽鸡新品系在优质鸡配套系中应用是可行的.  相似文献   

转基因苜蓿研究进展   总被引：4，自引：0，他引：4  

胡清泉  玉永雄 《中国草地》2005,27(5):58-62

就苜蓿基因工程在育种上的研究、转基因技术在苜蓿上的进展做了简要介绍。  相似文献   

山区农业面源污染的防治途径及对策     

喻尚其刘光德  李其林赵中金 《四川畜牧兽医学院学报》2005,3(4):1-4

结合重庆市地形地貌特点和山区农业面源污染发生的因子，提出山区农业面源污染的防治对策。一方面必需结合当地的自然环境条件和经济发展水平提出农业面源污染的防治规划；另一方面必需结合生态农业建设这一主线，使物质、能量在生态系统内良性循环。紧紧抓住产生农业面源污染源的因子——化肥、农药、农田秸杆、畜禽粪便、农村生活废物等，提出科学的防治和减少途径。  相似文献   

吉林省荒地资源生态适宜性评价     

张妍  尚金城  于相毅 《干旱区资源与环境》2003,17(3):35-38

本文从吉林省荒地资源的实际出发 ,在荒地资源生态适宜性评价基本理论的指导下 ,采用地理信息系统方法 ,对吉林省荒地资源进行了生态适宜性评价。通过空间数据和属性数据的融合、分析、处理 ,可以直观获得荒地资源的生态适宜度等级 ,从而提高评价效率 ,为荒地资源的合理开发利用提供科学依据。  相似文献   

乌梁素海湿地环境与资源地理信息系统的研究   总被引：6，自引：0，他引：6  

尚士友  吴利斌  郁志宏  岳海军  李洁  马清艳 《干旱区资源与环境》2003,17(5):59-64

本系统建立乌梁素海湿地基础地形库、生态库、资源库、工程档案库、遥感影像库等基础数据库及信息发布系统 ,使 GPS、GIS、RS紧密结合 ,同时实现了专家决策支持系统与地理信息系统之间的一体化集成。本系统可以为乌梁素海湿地生态治理与资源开发提供全方位的数据信息及其管理 ,对生态工程规划与实施方案进行优化设计 ,并提供实时监控服务。  相似文献   

Increased expression of CD40 ligand by peripheral blood mononuclear cells in patients with systemic lupus erythematosus     

LI Yu-jie  LI You-ji  YE Ren-gao  KONG Qing-yu  DONG Xiu-qing  YU Xue-qing 《园艺学报》2003,19(4):514-517

AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE.  相似文献   

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese     

ZHOU Shu-lu  YE Ren-gao  LIU Xiao-bo  ZHANG Hong  XU Han-shi  DU Yong  LI You-ji  YANG Nian-sheng  YANG Xiao  YU Xue-qing 《园艺学报》2003,19(6):782-785

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.  相似文献   

Using antisense nucleic acid technology to study the influence of POLκ on genetic stability     

LUO Yue-qiu  YU Ying-nian 《园艺学报》2003,19(9):1153-1156

AIM:To establish cell line FL-POLκ^- and to study the role ofPOLκ(polymerase kappa) on genetic stability.METHODS:A mammalian expression vector expressing antisense POLκ gene fragment pMAMneo -amp^--POLκ was constructed by cloning the 1 690-1 918 fragment of POLκ gene into the mammalian expression vector pMAMneo-amp^- in antisense orientation. FL cells were fransfected with this antisense RNA expressing vector and selected by G418. Based on the shuttle-plasmid pZ189, the mutation assay was made.RESULTS:The spontaneous mutation frequency of supF tRNA gene in the plasmid replicated in the FL- POLκ^- was 11.2×10^-4, while it was 4.9×10^-4 and 3.7×10^-4 in the control cells FL and FL-M, respectively.CONCLUSION: POLκ playes an important role in maintenance of genetic stability.  相似文献