Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (Procyon lotor)     

Kazuki Komatsu  Tsugumi Iwasaki  Kosuke Murata  Hideaki Yamashiro  Valerie Swee Ting Goh  Ryo Nakayama  Yohei Fujishima  Takumi Ono  Yasushi Kino  Yoshinaka Simizu  Atsushi Takahashi  Hisashi Shinoda  Kentaro Ariyoshi  Kosuke Kasai  Masatoshi Suzuki  Maria Grazia Palmerini  Manuel Belli  Guido Macchiarelli  Toshitaka Oka  Manabu Fukumoto  Mitsuaki A. Yoshida  Akifumi Nakata  Tomisato Miura 《Reproduction in domestic animals》2021,56(3):484-497

Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, we have established an archive system of livestock and wild animals from the surrounding ex-evacuation zone. Wildlife within the alert zone have been exposed to low-dose-rate (LDR) radiation for a long continuous time. In this study, we analysed the morphological characteristics of the testes and in vitro fertilization (IVF) capacity of cryopreserved sperm of racoons from the ex-evacuation zone of the FDNPP accident. The radioactivity of caesium-137 (¹³⁷Cs) was measured by gamma-ray spectrometry, and the measured radioactivity concentration was 300–6,630 Bq/kg in the Fukushima raccoons. Notably, normal spermatogenesis was observed in the seminiferous tubules of the testes, with the germinal epithelium composed of a spermatogenic cell lineage with no evident ultrastructural alterations; freeze-thawing sperm penetration ability was confirmed using the interspecific zona pellucida-free mouse oocytes IVF assays. This study revealed that the chronic and LDR radiation exposure associated with the FDNPP accident had no adverse effect on the reproductive characteristics and functions of male raccoons.  相似文献   

Growth Promotion of Spinach by Fluorescent Pseudomonas Strains under Application of Organic Materials     

Yasufumi Urashima  Yuko Suga  Kaneaki Hori 《Soil Science and Plant Nutrition》2005,51(6):841-847

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Antioxidative catechol lignans converted from sesamin and sesaminol triglucoside by culturing with Aspergillus     

Miyake Y  Fukumoto S  Okada M  Sakaida K  Nakamura Y  Osawa T 《Journal of agricultural and food chemistry》2005,53(1):22-27

Sesamin and sesaminol triglucoside in sesame seeds are major lignans that display an abundance of biological activities. Although their antioxidative activity in vitro is weak, they have been reported to suppress oxidative stress in vivo. We investigated the production of new antioxidative lignans from sesame lignans by culturing with the genus Aspergillus to enhance the function of food materials. Media containing sesamin or sesaminol triglucoside increased antioxidative activity for DPPH radical scavenging by culturing with Aspergillus usamii mut. shirousamii RIB2503. The antioxidative lignans in sesamin medium were identified as sesamin 2,6-dicatechol and episesamin 2,6-dicatechol. Those in sesaminol triglucoside medium were identified as sesaminol 6-catechol and episesaminol 6-catechol, which are novel antioxidative lignans. It is suggested that they may exhibit higher antioxidative activity than sesamin and sesaminol triglucoside because they have the catechol functional moiety.  相似文献   

Erythropoietin-producing cells in the liver of ICR-derived glomerulonephritis (ICGN) mice     

Yamaguchi-Yamada M  Akashi N  Goto Y  Anan S  Yamamoto Y  Ogura A  Manabe N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):65-68

The ICR-derived glomerulonephritis (ICGN) mouse is an appropriate model for anemia associated with chronic renal disorder (CRD). Insufficient renal production of erythropoietin (EPO) induces the anemia associated with CRD. EPO mRNA is expressed in both kidneys and liver of progressing-stage ICGN mice. Hypoxic stimulation induced the EPO mRNA expression in the liver as well as in the kidneys of ICGN mice. The localization of EPO-producing cells in the liver remains controversial. Present study using an amplified in situ hybridization technique identified that nonparenchymal cells were the source of hepatic EPO production in ICGN mice under both normoxia and hypoxia.  相似文献   

Growth performances of F1 angus plus calves grazing on pasture in Hawaii’s tropical climate     

Yang J  Ferreira R  Duponte MW  Fukumoto GK  Zhao B 《Tropical animal health and production》2009,41(4):593-598

Angus Plus cattle offer advantages for heat tolerance and forage utilization by introduction of Brangus and Brahman to Angus. To evaluate its adaptability in Hawaii Islands, we reported the growth performances of 213 F1 Angus Plus calve grazing on pasture. Least-square means of pre-weaning ADG ranged from 1,087 to 1,167 g in bull calves and from 1,030 to 1,048 g in heifer calves. The 205 d-adjusted weaning weight were 226 to 285 kg in bulls and 214 to 252 kg in heifers. The birth weight and hip height at birth were significantly correlated with weaning weight, 205 d-adjusted weaning weight, hip height at weaning and pre-weaning ADG (P < 0.01). Sire group significantly influenced pre-weaning growth performances through interaction with sex of calf. Bull calves from sire group of high growth were 1.0–3.8 kg heavier in birth weight than the bull calves from other sires (P < 0.001). Sire group x sex interaction was significant (P < 0.05) for calf birth weight, 205-d adjusted weaning weight and pre-weaning ADG. Sire group also played a significant role in hip height at birth (P < 0.05). Selections of the sires preferable for growth significantly improved calf pre-weaning growth performances.  相似文献   

Synthesis and insect antifeedant activity of aurones against Spodoptera litura larvae     

Morimoto M  Fukumoto H  Nozoe T  Hagiwara A  Komai K 《Journal of agricultural and food chemistry》2007,55(3):700-705

A series of aurones were prepared from various phenols via phenoxy acetic acids and coumaranones and evaluated for insect antifeedant activity against the common cutworm (Spodoptera litura). The naturally occurring aurone was most active at an ED50 of 0.12 micromol/cm2. The synthetic precursor, coumaranones, showed that the introduction of methoxyl and methyl groups to the benzene ring increased insect antifeedant activity. Similarly, the tested aurones showed that the introduction of methoxyl group to the A and/or B rings increased the insect antifeedant activity, but 4,5,6- and 3',4',5'-trisubstituted compounds did not show this activity in this test. The hydroxylation of aurones in the B ring should be disadvantageous for insect antifeedant activity against S. litura. Although the melting points did not correlate well with the insect antifeedant activity, compounds that were nearly inactive had high melting points. A significant correlation was noted between biological activity (pED50) and a hydrogen-bonding parameter calculated from the Rf value obtained from SiOH thin-layer chromatography and a lipophilicity parameter (log k) calculated from the retention time in ODS high-performance liquid chromatography. The respective correlation coefficients (r) were -0.83 and -0.70. The introduction of alkoxy and alkyl groups along with adequate hydrogen bonding seems to contribute to the antifeedant activity of the compounds tested.  相似文献   

京津冀区域收入差距与环境质量关系的实证研究     

申伟宁  福元健志  张韩模 《干旱区资源与环境》2017,31(11):39-43

文中利用京津冀区域1994-2014年间的经济与环境数据,在收入差距视角下扩展EKC假说,对收入差距与环境质量间的关系实证检验。结果发现:1994-2004年间京津冀区域收入差距与污染物排放之间呈现的关系并不一致,且显著性不强;而2005-2014年间京津冀区域收入差距与污染物排放呈现正向驱动关系,表明2005年之后收入差距的影响作用加剧了京津冀区域环境质量的恶化。究其原因,与2004年"廊坊共识"达成以后,京津冀区域产业转移有着密切关系。因此,为保障京津冀协同发展国家重大战略实施,应抑制区域间收入差距扩大,防止"污染转移假说"效应,以实现经济与环境的协调发展。  相似文献   

Effect of RNA interference of BID and BAX mRNAs on apoptosis in granulosa cell-derived KGN cells     

Sai T  Matsuda F  Goto Y  Maeda A  Sugimoto M  Gao HM  Kabir AK  Li JY  Manabe N 《The Journal of reproduction and development》2012,58(1):112-116

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Carcass quality and meat tenderness of Hawaii pasture-finished cattle and Hawaii-originated, mainland feedlot-finished cattle     

Kim YS  Fukumoto GK  Kim S 《Tropical animal health and production》2012,44(7):1411-1415

The objective of this study was to compare the carcass quality and meat tenderness of Hawaii cattle finished on subtropical pasture with those of mainland US feedlot-finished cattle that were shipped from Hawaii after weaning. Rib-eye steak samples were collected from 30 feedlot-finished cattle harvested at a slaughter house in Washington State, USA and from 13 subtropical pasture-finished cattle harvested at a local slaughter house in Hawaii, then shipped to meat science laboratory at the University of Hawaii, Manoa. Samples were aged for 2?weeks at 4°C and frozen for later proximate analysis and meat tenderness measurement. Feedlot-finished cattle had significantly heavier carcass weight (353 vs 290?kg) and thicker backfat (13.5 vs 6.6?mm), but no significant difference was observed in rib-eye area between the two groups. Marbling score (Small) and United States Department of Agriculture quality grade (Choice) of the pasture-finished beef were not significantly (P?相似文献   

Changes in the localization of type I, III and IV collagen mRNAs in the kidneys of hereditary nephritic (ICGN) mice with renal fibrosis     

Uchio K  Manabe N  Yamaguchi-Yamada M  Goto Y  Yamamoto Y  Ogura A  Miyamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):123-128

Renal fibrotic change, extreme accumulation of extracellular matrix (ECM) components in glomeruli and tubulointerstitum, is one of the characteristic features of ICR-derived glomerulonephritis (ICGN) mice. Decreased degradation of ECMs by matrixmetalloproteinases was demonstrated in kidneys of ICGN mice. To determine the balance between production and degradation of ECMs in kidneys of ICGN mice, we examined expression of mRNAs of ECMs in those. To demonstrate the localization of type I, III and IV collagen mRNAs in kidney sections of ICGN and control ICR mice, in situ hybridization using digoxigenin-labeled oligonucleotide antisense probes for procollagen-alpha(1) (I), -alpha(1) (III) and -alpha(1) (IV) mRNAs, respectively, was performed. Negative or trace expressions of type I and III collagen mRNAs were observed in the kidneys of control mice, but stronger expressions of those were seen in glomeruli and injured renal tubules of the kidneys of ICGN mice. Moderate expression of type IV collagen mRNA was demonstrated in a part of glomeruli and renal tubules of both control and ICGN mice, and no remarkable difference was seen between them. Severe renal fibrosis, extreme accumulation of interstitial type I and III collagens is caused by increased production and decreased degradation in the kidneys of ICGN mice. Thus, the profiles of metabolism between interstitial and membranous collagens may be different in the kidneys of ICGN mice, and excessive production of interstitial collagens may be the dominant cause of renal disease in them.  相似文献