Detection and analyses of coagulation and fibrolysis factor mRNA levels in tissue of hepatocellular carcinoma patients     

ZHOU Qi  LI Shao-qiang  LIANG Li-jian  PENG Bao-gang  ZHEN Yu-yang  SU Cai-kun 《园艺学报》2006,22(7):1348-1352

AIM:To detect the mRNA expression of tissue factor (TF), urokinase-type plasminogen activator (u-PA) and urokinase-type plasminogen activator receptor (u-PAR) in the samples of hepatocellular carcinoma (HCC) and adjacent tissue, and to elucidate their association with clinical significance. METHODS:The mRNA levels of TF, u-PA and u-PAR in 27 human HCC tissue samples with their adjacent tissue samples and 27 normal liver tissues were detected by RT-PCR. The relationship between the mRNA expression and their clinic-pathological data were also analyzed. RESULTS:The expressive rate of TF, uPA and uPAR in HCC tissue samples was 62.96% (17/27), 70.37% (19/27), 77.78% (21/27), respectively and relative expression intensity of TF, uPA and uPAR were 0.567±0.268, 0.964±0.458 and 0.784±0.322, respectively, which were significantly higher than those in adjacent tissue group and normal liver group (P<0.05). TF, uPA and uPAR mRNA levels were all significantly higher in larger size and in the invasive and metastatic subgroup (P<0.05). There had positive relationship between the mRNA expressions of these three factors. CONCLUSION:The results indicate that TF, uPA and uPAR might play an important role in hepatic carcinogenesis, invasiveness and metastasis.  相似文献   

Relationship between uPA and NF-κB p65 expression and its clin ical significance in breast cancer     

WANG Hong  WU Jin-min  CAO Hou-jun  ZHOU Wen-peng  FANG Yong  ZHANG Xing  ZHENG Shu 《园艺学报》2006,22(6):1218-1221

AIM:To evaluate the relationship between u PA and NF-κB p65 expression and its clinical significance in breast cancer.METHODS:The uPA mRNA was measured by real-time fluorescent quan t itative PCR in 46 cases of breast cancer tissues and their adjacent counterparts .NF-κB p65 were measured using immunohistochemistry.RESULTS:The expression of uPA gene was elevated in 63% of cases ,and there was a strong correlation between NF-κB p65 and uPA expression (r ＝0.451，P<0.01).In addition,the expression of uPA was associated with num bers of axillary lymph node involvement and tumor size.However,no significance was found in uPA expression in the age,pathological type and distant metastasi s organ classification.CONCLUSION:The uPA gene is highly expressed in most of the brea st cancer,and there is strong correlation to NF-κB p65 expression,number of l ymph nodes involvement and tumor size.  相似文献   

Effects of homocysteine on apoptosis and expression of caspases-3 in cultured human vascular smooth muscle cells     

WANG Xiang-gui  FENG Yi-bai  ZENG Qiu-tang  CAO Lin-sheng  ZHOU Zhi-ming  ZHU Li-min 《园艺学报》2006,22(4):707-710

  相似文献   

Effect of endogenous H2S on pulmonary hypertension during acute lung injury induced by LPS and its interaction with NO     

ZHOU Xiao-hong  WEI Peng  LING Yi-qun  DAI Hong-yan  HUANG Xin-li  LING Yi-ling 《园艺学报》2006,22(4):715-721

AIM: To investigate the effect of H2S on pulmonary artery hypertension during acute lung injury induced by LPS and the interaction between the systems of hydrogen sulfide (H2S)/cystathionine-β-lyase (CSE) and nitric oxide (NO)/nitric oxide synthase (NOS) in this process. METHODS: Seventy-two adult male rats were randomly divided into four groups: control group, LPS group, LPS+L-NAME group and LPS+propargylglycine (PPG) group. Mean pulmonary artery pressure (mPAP) of each rat was examined at 2 h, 4 h, 6 h and 8 h after treatment. H2S and NO contents in plasma, NO content, iNOS, cNOS and CSE activity in lung were measured at 4 h or 8 h after treatment, respectively. Expression of iNOS in lung tissue was also detected by immunohistochemistry technique, and the injury of lung was evaluated with morphological changes under microscope. RESULTS: LPS could induce severe lung injury, and mPAP, NO content, iNOS activity and its protein expression in LPS group significantly increased, but cNOS activity, H2S content and CSE activity decreased compared with those of control group. Administration of L-NAME before LPS could attenuate the changes induced by LPS. Pre-administration of PPG, a CSE inhibitor, exacerbated the injury by LPS, but there was no prominent variation in cNOS activity. CONCLUSION: Reduced endogenous H2S could increase pulmonary artery hypertension during acute lung injury induced by LPS. There is a negative effect between H2S/CSE system and NO/NOS system in this process.  相似文献   

Exogenous carbon monoxide protects against the lung injury induced by ischemia-reperfusion of hind limbs in rats     

ZHOU Jun-lin  LING Yi-ling  SU Qing-jun  Kang Nan  LU Shi-bao  GUAN Li 《园艺学报》2006,22(2):303-306

AIM: To study the mechanism of protective effect of exogenous carbon monoxide (CO) in the lung injury induced by ischemia-reperfusion (IR) of hind limbs in rats. METHODS: Thirty-two SD rats were randomly divided into 4 groups: control, control+CO, IR and IR+CO. A rat model of ischemia in hind limbs and the reperfusion lung injury was made. The rats in IR+CO and control+CO groups were exposed to air containing 2.5×10-8 CO for 1 h before reperfusion or the corresponding control time point, while the other two groups were exposed to the routine air. The lung tissue structure, polymorphonuclear leukocyte (PMN) count, wet-to-dry weight ratio (W/D), malondialdehyde (MDA) content and the animal survival rate were observed. The carboxyhemoglobin (COHb) levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting. RESULTS: Compared to control, the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly increased in IR group. Compared with the IR group, the blood COHb level was significantly increased and the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly decreased in IR+CO group. CONCLUSION: These data suggest that exogenous CO attenuate limb IR-induced lung injury by down-regulatiny ICAM-1 expression and suppressing PMN sequestration in the lung following limb IR in rats.  相似文献   

Biological characters of mesenchymal stem cells of human umbilical cord blood     

ZHOU Dun-hua  WEI Jing  HUANG Shao-liang  FANG Jian-pei  BAO Rong  WU Yan-feng  LI Yang 《园艺学报》2006,22(8):1606-1609

AIM：To study the isolation,expansion and purification of mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB),and investigate some biological identities of MSCs.METHODS：(1) MSCs of UCB,adult bone marrow (BM) and fetus BM were isolated by centrifugation with Ficoll,and the different kinds of MSCs were observed everyday.(2) Surface markers of MSCs were identified by flow cytometry.(3) The level of HGFs (TPO,SCF,FLT-3L,IL-6) secreted by different sources of MSCs was checked by ELISA method.RESULTS：(1) No difference in morphology of the colonies between UCB MSCs and BM MSCs was observed.However,the mononuclear cells needed in culture of UCB MSCs was about 3 times more than that in culture of BM MSCs.The times of UCB MSCs colony formation and confluencing were longer than that of BM in primary culture.(2) After passaged,there was no significant difference in the proliferation rates of 3 kinds of MSCs.Only 4 of 15 UCB samples contained a homogeneous population of MSCs.(3) UCB MSCs shared the same markers with BM MSCs.Neither hematopoietic marker nor immunologic recognition antigens were expressed.(4) The level of hematopoietic growth factors (HGFs) secreted by 3 kinds of MSCs was similar.CONCLUSIONS：(1) MSCs were isolated from UCB,but the amount of MSCs in UCB was smaller than that in BM,and just seldom samples of UCB contained homogeneous MSCs.(2) MSCs from UCB and BM shared the same biological characteristics,such as proliferation ability,surface markers,immunophenotypes and HGFs secretion.  相似文献   

地面高光谱和低空遥感监测小麦白粉病初探   总被引：4，自引：1，他引：4  

乔红波  周益林  白由路  程登发  段霞瑜 《植物保护学报》2006,33(4):341-344

利用手持式高光谱仪和基于数字技术的低空遥感系统，对不同严重度小麦白粉病冠层光谱反射率进行了测定，同时调查病情指数，分析不同时期地面平台光谱反射率与病情指数及低空遥感平台反射率与病情指数（disease index，DI）、归一化植被指数（normalized difference vegetable index，NDVI）相关性。结果表明，地面光谱测量冠层光谱反射率和低空遥感数字图像反射率与小麦白粉病病情指数在灌浆期有显著的相关关系，就地面测量结果而言，近红外波段的相关性高于绿光波段，相关系数分别为0．79和0．54；低空遥感数字图像红、绿、蓝三波段中，相关性依次降低，相关系数分别为0．79、0．75和0．62；而且低空遥感图像与归一化植被指数也存在较好的相关关系，蓝、红、绿波段，相关系数依次为0．70、0．68和0．54。这表明利用遥感非破坏性监测小麦白粉痛有着良好的应用前景，对于小麦白粉病发生面积监测有重要意义。  相似文献   

云南、福建、湖南烟区烟草花叶病主要病毒种类检测及黄瓜花叶病毒亚组鉴定   总被引：10，自引：0，他引：10  

刘勇  莫笑晗  余清  顾钢  黄石旺  夏玉珍  周雪平 《植物病理学报》2006,36(4):310-313

 采用抗原直接包被和双抗体夹心酶联免疫吸附测定法(ELISA)对采自云南、福建、湖南烟区烟草花叶病样品进行了病毒种类检测,利用三抗体夹心ELISA对黄瓜花叶病毒(Cucumber mosaic virus,CMV)的亚组类型进行了鉴定。在云南采集的520个花叶病样品中,烟草花叶病毒(Tobacco mosaic virus,TMV)、CMV和马铃薯Y病毒(Potato virus Y,PVY)总检出率分别为71.74%、55.01%和6.35%;在福建采集的150个花叶病样品中,TMV、CMV和PVY的总检出率分别为94%、24.66%和8.00%;在湖南采集的74个花叶病样品中,TMV、CMV和PVY的总检出率分别为58.11%、51.35%和2.70%。部分样品为2种以上病毒复合侵染。云南、福建和湖南采集的64个CMV阳性样品中,属亚组Ⅰ的样品为57个,占89.1%;属亚组Ⅱ的样品为10个,占15.6%;其中3个样品为亚组Ⅰ和亚组Ⅱ的复合侵染。  相似文献   

采用AFLP技术分析稻曲病菌的遗传多样性Ⅰ:同一块稻田中稻曲病菌的遗传结构   总被引：7，自引：1，他引：7  

潘雅姣  樊金娟  付彬英  王洁  徐建龙  陈红漫  黎志康  周永力 《植物病理学报》2006,36(4):337-341

 采用AFLP (Amplified fragment length polymorphism)技术分析了来自北京昌平同一块稻田中不同水稻品种和育种中间材料上稻曲病菌(Ustilaginoidea virens)的遗传多样性。从256对EcoRⅠ和MseⅠ引物中选择30对扩增40个菌株。结果表明,同一块稻田中稻曲病菌菌株间的相似性系数达0.72以上,来自同一小区的多数菌株能聚成亚类;发现从同一水稻品种分离的菌株没有特异性的AFLP谱带。初步推断稻曲病菌与水稻品种不存在明显的专化性互作。  相似文献   

植物提取物、碳酸钠和碳酸氢钠室内对杨树溃疡病菌生长的抑制作用     

唐静  周立刚  曹晓冬  谢光辉  隋鹏  谈满良  周亚明 《植物病理学报》2006,36(5):446-453

 采用菌丝生长速率法用金银花的花、黄花蒿的地上部分、蓝桉果实和黄柏果实的乙醇提取物及其不同极性的组分对杨树溃疡病菌菌丝生长的抑制作用进行了测定。以黄柏果实的抗菌活性最强,其次是金银花的花。抗菌活性成分主要存在于金银花的正丁醇组分、黄花蒿的石油醚组分和乙酸乙酯组分、蓝桉的水部分、黄柏的正丁醇组分。当培养基中碳酸钠和碳酸氢钠的浓度分别为8g/L时(对应的pH值分别为10.24和7.71),菌丝生长抑制率分别为100.00%和79.68%。如果用1mol/L氢氧化钠溶液调节培养基的pH值至10.00,菌丝生长抑制率为40.58%。说明碳酸钠或碳酸氢钠对菌丝生长的抑制作用,一方面是由于改变了培养基的pH值,另一方面可能是由于碳酸根和碳酸氢根离子抑制了菌丝的生长。  相似文献