Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.     

J Tetens  S A Barker  M Waguespack    G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.  相似文献   

Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.   总被引：3，自引：0，他引：3       下载免费PDF全文

M D Bierk  S A Dee  K D Rossow  S Otake  J E Collins    T W Molitor 《Canadian journal of veterinary research》2001,65(4):261-266

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.  相似文献   

一种估测亚麻出麻率的简便方法     

李明  杨学 《中国麻业科学》2001,23(1):9-10

统计分析显示亚麻茎中段的出麻率与单株出麻率相关极显著，可以在育种的单株选择过程中，利用茎中段的出麻率来快速估算单株出麻率，以减轻工作量，提高工作效率。  相似文献   

屠宰生猪的心，肝，肺检验初探     

曹阳  王明海张威 张蕊 《中国动物检疫》2001,18(2):31-31

宰后检验是整个肉品卫生检验工作中的重要环节，是宰前检疫的继续和补充。“四部”制定的《肉品卫生检验试行规程》和国家国内贸易局制定的《肉品品质检验规程》都明确规定必须进行心、肝、肺检验，它是宰后检验的重要组成部分和主要着眼点之一。这些卖质器官的检验可以作为我们综合判检的主要依据。另外，我国广大人民群众比较喜食内脏器官，这更要求我们对心、肝、肺进行严格的检验，防止食肉中毒，防止畜禽疫病传播，防止人畜共患传染病。１　心脏ｌ．１心脏检验　四部规程规定“检查心包及心肌并沿动脉管剖检心室及心内膜，同时注意血液…  相似文献   

干细胞与肿瘤研究进展     

刘雨潇  杨继建  张惠茹  窦忠英 《勤云标准版测试》2005,(6)

肿瘤是危害人类健康的恶性疾病,近年来越来越多证据显示肿瘤与干细胞有着密切的关系。肿瘤可能是干细胞在异常微环境中差异分化的结果。它与正常干细胞有相似的表面标志和生化特点。在此,综述了干细胞与肿瘤发生的关系及干细胞工程在肿瘤治疗中的应用前景,旨在为肿瘤发生发育的研究及干细胞在肿瘤治疗方面的应用提供理论依据。  相似文献   

酸化剂、益生素和寡糖在断奶仔猪饲料中的应用研究进展     

冯仁勇  向阳 《饲料广角》2006,(4):38-40

1酸化剂、益生素和寡糖在断奶仔猪饲料中的应用1.1酸化剂在断奶仔猪饲料中的应用目前,养猪生产中普遍存在的一个难题就是仔猪断奶后消化机能紊乱,导致生长受阻甚至死亡,原因之一是仔猪不能分泌足够的胃酸,胃中的pH值多在4.2以上;加上一些饲料原料的强缓冲能力(Bolduan,等,1988)  相似文献   

西南交通大学郫县新校区绿化环境设计中交流氛围的营造   总被引：3，自引：1，他引：2  

刘扬 《林业科技开发》2005,19(1):81-84

现代化教育的发展使得高等学校的功能不断地扩展，对内对外交流与合作的功能正在日益显现。这要求在高校校园绿化环境设计中体现和满足这一功能。提出了在绿化环境设计中营造交流氛围的原则，并在西南交通大学郫县新校区绿化环境设计中对交流氛围的营造作了初步探索和实践。  相似文献   

红曲霉色素的提取     

张文芳  蒋光正  李薇  刘若男 《东北农业大学学报》1990,(1)

本实验确立了一种新的红曲霉色素提取方法:以红曲米为原料,乙醇为提取剂,得到的提取液再以正己烷提取分离。结果表明,此法所得的红曲霉色素是水溶性的,宜于用作食品添加剂。此外,还对影响提取的因素进行了探讨,并提供了最佳提取方案。  相似文献   

中国平背树螽属两新种(直翅目:螽斯科:树螽亚科)     

杨集昆  康乐 《中国农业大学学报》1990,(4):420-422

描述了平背树螽属2新种,定名为双刺平背树螽(Isopsera bicuspidota Yang etKang)和广西平背树螽(I.guangziensis Yang et Kang)。  相似文献   

Outcome of limb amputations in wapiti: 13 cases (1995-2001).          下载免费PDF全文

T D Butt  A M Cruz  J V Bailey    W H Crawford 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(12):936-939

The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti.  相似文献