排序方式： 共有212条查询结果，搜索用时 15 毫秒
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小麦淀粉糊化特性是评价小麦加工品质和品质育种的重要指标之一。对不同年份和地点5个环境生长的矮孟牛姊妹系及其衍生系共109份材料的RVA参数(峰值黏度、低谷黏度、稀澥值、最终黏度、回生值和糊化温度)观测, 并利用混合线性模型分析其与覆盖小麦全基因组的971个DArT (Diversity Array Technology)标记的关联性。结果表明, 分布于19条染色体上的70个DArT标记与上述RVA参数显著关联(P≤0.001), R2值范围是0.2%~23.3%。2A、2B和2D染色体上标记与多个RVA参数都有关联, 且效应值较大。这为淀粉湖化特性的分子标记辅助选择提供了重要的信息。 相似文献
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LIU Yun-bo HU Peng-fei WANG Hai-long ZHANG Jin-you LI Chang-sheng ZHANG Gui-xue* 《东北农业大学学报(英文版)》2005,12(1):68-73
Research on development of fetal ovary was traced to late 70 s. Histological study on fetal horse ovarian germinal epithelium was conducted[1], followed research on germcell proliferation was carried out[2]. In the late 80s,development of 37-118 dfetalrhesus ovaries were studied through continuous ultrathin sec-tion [3]. Histological study on late fetal rhesus ovaries were conducted [4]. It was believed that fetal ovaries had ability to biosynthesis estrogen and response to gonadotrophin after… 相似文献
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REN Gui-ping QU Juan-juan PEI Fu-cheng LI Jing-peng LIU Xiang-yu LI Lu WANG Jun-wei* 《东北农业大学学报(英文版)》2005,12(1):11-13
To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic ex-pression vector of DuIFN-α was constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the proteingene of DuIFN-α was cloned from pMD-18-duIFN-α recombinant. The gene was then inserted to pGEM-T vectorand identified by restriction endonuclease analysis and sequencing. DuIFN-α was ligated with the prokaryotic expres-sion vector of pET30 a, then transformed into BL21 (DE3) plysS. The best inducing time and IPTG concentration for the expression of this recombinant protein was tested through the expression of the positive recombinant with differ-ent time span and different IPTG concentration. Lots of the protein of DuIFN-α were expressed in BL21 (DE3)plysS with 1 mmol·L-1 IPTG for 4 hours and its molecular weight for 34 000. 相似文献
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茶树内生球座菌的鉴定及遗传多样性分析 总被引：1，自引：0，他引：1
以分离自茶树和山茶等植物的25个内生球座菌分离物为材料,采用形态学、18S rDNA序列分析和ITS1-5.8S-ITS2序列分析相结合的方法,对这些球座菌分离物进行鉴定;并应用简单序列重复区间扩增多态性(inter-simple sequence repeat polymorphism, ISSR)对这些菌株的遗传多样性进行分析.结果表明:所获得的25个球座菌分离物虽然在PDA培养基上的菌落形态和生长速度存在较明显的差异,但形态学特征、18S rDNA序列和ITS1-5.8S-ITS2序列分析表明这些菌株均属于芒果球座菌(Guignardia mangiferae).通过ISSR分析,从35个随机引物中筛选出10个表现多态性的引物,扩增后共获得88个ISSR标记,其中多态性标记76个,占86.3%.以相异距离0.29为标准,可以将此25个菌株划分为3个遗传聚类群,在PDA上形态相似、生长速度相近的菌株聚为一群;ISSR分析结果还显示芒果球座菌在山茶中具有更为丰富的遗传多样性. 相似文献
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XU Qijiang CHEN Dian TONG Youli and LI Yuhua* Research Institute of Flower Biotechnology Northeast Forestry University Harbin China College of Horticulture Northeast Agricultural University Harbin China 《东北农业大学学报(英文版)》2007,14(1):9-13
Three pairs of primers were designed and synthesized from nucleotide sequences of garlic latent virus (GLV), onion yellow dwarf virus (OYDV), and leek yellow stripe virus (LYSV) by using PCR primer design software. The expected fragments about 170 bp, 287 bp, and 191 bp were amplified by RT-PCR for GLV, OYDV, and LYSV, respectively in disease-infected plants of potato onion (Allium cepa L., Aggregatum group), but such fragments were not obtained from healthy-looking plants and virus-free seedlings of shoot-tips. The amplified products of GLV, OYDV and LYSV were cloned into pGEM-T vectors, and transformed into Escherichia coli. JM109. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank by performing a NCBI BLAST. The analysis showed that their homology attained 75% to 90%,89.5% to 96.1%,and 91.6% to 96.3% in GLV, OYDV, and LYSV, respectively. The total RNA of 6.34 μg·μL~(-1) from infected plants was diluted to a series of 10~(-1) to 10~(-5) and the detection sensitivity of RT-PCR was 10~(-4) (about 4 ng). Thus, a method of identification and detection by RT-PCR of GLV, OYDV, and SLYV was established. 相似文献
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随着城镇化的快速推进和城市规模的扩张,城市道路景观越来越受到人们的重视,如何将地域文化融入城市道路景观成了提升道路景观的突破口。本文以内江市学院路景观设计为例,阐述了蕴含地域文化的城市道路景观的概念及其重要性,并通过"主题介入"式的分段景观设计,探索了城市道路景观的地域文化表达方法。旨在提高人们对蕴含地域文化的城市道路景观的重视,并通过合理的方法进行设计,提升城市道路景观的品质与内涵,营造具有个性与特色的城市景观。 相似文献
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QIN Qingfeng HE Wei LIANG Jun ZHANG Xingyao * . College of Resources Environment of Beijing Forestry University Beijing P.R.China . Research Institute of Forest Ecology Environmental Protection CAF Beijing P.R.China 《中国林业科技(英文版)》2005,4(2):87-94
1 INTRODUCTIONPlants have several defense mechanisms againstpathogenic attack, one of which is through proteaseinhibitors proteins. Cystatins are a group of proteinsthat act as inhibitors of cysteine proteinases and widelyexist in plants, animals, and microbes. Plant cystatinshave been well characterized in rice (Abe K, et al,1985); maize (Abe M, et al, 1988); cowpea(FernandesK V S, et al, 1991); sunflower (Kouzuma Y, et al,1996); sorghum(Li C, et al, 1996); soybean(MisakaT, et al, 1… 相似文献
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园林绿化环境越来越好,但是植物应用中也出现了一些不足之处。本文通过园林植物应用现状不足的调查分析,从而来说明园林植物的应用要点。 相似文献
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