Evidence that oxidative stress is higher in replacement gilts than in multiparous sows          下载免费PDF全文

C. Roy  M. Lavoie  G. Richard  A. Archambault  J. Lapointe 《Journal of animal physiology and animal nutrition》2016,100(5):911-919

The recent success obtained in term of increasing the litter size of sows has not correlated with a reduction of replacement rate. There is thus an increased economic demand for gilts with optimal reproductive potential and longevity. Unfortunately, replacement gilts are known to be more susceptible to diseases and less productive than multiparous sows. Interestingly, reproductive performance, resistance to diseases and longevity could all be largely affected by oxidative stress. To investigate whether oxidative stress conditions could account for the poor longevity of gilts, three distinct groups of conventional Yorkshire × Landrace sows were formed based on their similar age and parity (gilts, second parity sows as well as fourth to fifth parity sows). All animals were slaughtered during the post‐ovulatory period, and blood as well as tissue samples were collected. Biomarkers of oxidative damage to proteins (carbonyls) and DNA (8‐OHdG) were analysed in samples. Specific mRNA expression of major antioxidants such as glutathione peroxidases 1, 3 and 4 (GPx1, GPx3, GPx4) as well as superoxide dismutases 1 and 2 (Sod1, Sod2) were monitored in liver and kidney samples by quantitative RT‐PCR. Specific enzymatic activities of both GPx and SOD were measured by spectrophotometric assays. The plasma concentration of protein carbonyls was significantly different between the three groups with the highest concentration being observed in gilts (p ≤ 0.001). The mRNA expression levels of GPx1 and GPx4 were also significantly increased in the liver of gilts when compared to multiparous sows (p ≤ 0.05). SOD2 enzymatic activity was found to be higher in the liver of gilts than multiparous sows (p ≤ 0.05). Taken together, these results indicate that replacement gilts sustain significantly higher oxidative conditions than multiparous sows. Current findings may contribute to the design of nutritional regimens that will increase the productivity of gilts by counteracting oxidative stress.  相似文献   

Clinical and in vitro efficacy of amoxicillin against bacteria associated with feline skin wounds and abscesses   总被引：1，自引：0，他引：1  

Roy J  Messier S  Labrecque O  Cox WR 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(6):607-611

A clinical trial involving 122 cats with infected skin wounds or abscesses presented to 10 veterinary clinics was conducted to evaluate the efficacy of 2 oral amoxicillin drug products (a paste and a suspension). A 2nd objective of the study was to identify bacteria involved in such infections and verify their in vitro sensitivity to amoxicillin. Samples of wound exudate were harvested at the time of presentation and submitted for aerobic and anaerobic culture. The sensitivity to amoxicillin of isolates thought to be infecting agents was tested, using a standard minimum inhibitory concentration method. Pasteuralla multocida and obligate anaerobes of the genera Prevotella, Fusobacterium, and Porphyromonas were the most frequently isolated pathogens. Overall, their in vitro susceptibility to amoxicillin was very good. Both drug products were clinically efficacious with a global success rate of 95.1% for cats administered oral amoxicillin at 11-22 mg/kg bodyweight (mean 13.8 mg/kg bodyweight) twice daily for 7 to 10 days.  相似文献   

A Fish of Weedy Waters: Golden Shiner Biology and Culture          下载免费PDF全文

Anita M. Kelly  Luke A. Roy 《Journal of the World Aquaculture Society》2016,47(2):152-200

The golden shiner, Notemigonus crysoleucas, is a major bait and forage fish species in the USA. Farmers produce millions of golden shiners annually that are distributed live, many across state boundaries. Native over much of the eastern and central USA, this species is often present but rarely abundant in natural systems, as fish density is apparently controlled by predation rather than by food resources. Aquaculture of the golden shiner is a century‐old practice and culture methods have evolved over time, resulting in today's farm‐raised, certified specific pathogen– and aquatic nuisance species–free baitfish. Federal and state agencies and universities have a long history of developing culture methods and promoting baitfish culture to replace the indiscriminate and wasteful harvest of fish from natural waters. Despite advances in culture methods and the advantages of a farm‐raised species produced under controlled and biosecure conditions, golden shiner farming is only marginally profitable and remaining farmers survive on farm equity and increased market share from others exiting the business. Ironically, increasing regulations and restrictions leading to a reduction in the supply of farm‐raised baitfish could drive anglers back to harvesting bait from natural waters, the very concern that initiated government support for baitfish culture a century ago.  相似文献   

Response of Pacific White Shrimp,Litopenaeus vannamei,to Three Sources of Solvent Extracted Soybean Meal     

Xuezhi Zhu  D. Allen Davis  Luke A. Roy  T. M. Samocha  J. P. Lazo 《Journal of the World Aquaculture Society》2013,44(3):396-404

Three sources of soybean meal (SBM) were biochemically characterized and evaluated in diets for juvenile Litopenaeus vannamei. Three biological techniques were utilized to evaluate the nutritional quality of the meals including an outdoor growth trial as well as determination of in vivo apparent digestibility and in vitro digestibility including pepsin digestibility (0.0002%), pH Stat degree of hydrolysis, and immobilized digestive enzyme assay (IDEA). The growth trial was conducted over a 10‐wk period using soybean meal as the primary protein source with each meal being included at 54–58% of the diet based on an equal protein inclusion. At the conclusion of the growth trial there were no significant differences between the test diets indicating equivalent biological availability under practical conditions. The in vivo digestibility trial utilized chromic oxide as the inert marker and 70:30 replacement techniques resulted in typical results. Apparent protein digestibility (APD) and apparent energy digestibility (AED) of the diets ranged from 66.8 to 80.2% and 65.4 to 74.7%, respectively. Ingredient digestibility values for protein and energy ranged from 78.8 to 93.5% and 60.5 to 85.0%, respectively. One of the three SBM evaluated had numerically lower protein digestibility and significantly lower energy digestibility than the other two meals .  相似文献   

Climate-driven range expansion and morphological evolution in a marine gastropod     

Hellberg ME  Balch DP  Roy K 《Science (New York, N.Y.)》2001,292(5522):1707-1710

Little is known about the phenotypic consequences of global climate change, despite the excellent Pleistocene fossil record of many taxa. We used morphological measurements from extant and Pleistocene populations of a marine gastropod (Acanthinucella spirata) in conjunction with mitochondrial DNA sequence variation from living populations to determine how populations responded phenotypically to Pleistocene climatic changes. Northern populations show little sequence variation as compared to southern populations, a pattern consistent with a recent northward range expansion. These recently recolonized northern populations also contain shell morphologies that are absent in extant southern populations and throughout the Pleistocene fossil record. Thus, contrary to traditional expectations that morphological evolution should occur largely within Pleistocene refugia, our data show that geographical range shifts in response to climatic change can lead to significant morphological evolution.  相似文献   

First record of a Hop stunt viroid variant on Nagpur mandarin and Mosambi sweet orange trees on rough lemon and Rangpur lime rootstocks     

P. Ramachandran  J. Agarwal  A. Roy  D. K. Ghosh  D. R. Das  Y. S. Ahlawat 《Plant pathology》2005,54(4):571-571

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Occurrence of Piscirickettsiosis-like syndrome in tilapia in the continental United States.     

Michael J Mauel  Debra L Miller  Eloise Styer  Deborah B Pouder  Roy P E Yanong  Andrew E Goodwin  Thomas E Schwedler 《Journal of veterinary diagnostic investigation》2005,17(6):601-605

From 2001 to 2003, tilapia (Oreochromis sp.) farms in Florida, California, and South Carolina experienced epizootics of a systemic disease causing mortality. The fish exhibited lethargy, occasional exophthalmia, and skin petechia. The gills were often necrotic, with a patchy white and red appearance. Grossly, the spleen and kidneys were granular with whitish irregular nodules throughout. Granulomatous infiltrates were observed in kidney, spleen, testes, and ovary tissues, but not in the liver. The granulomas contained pleomorphic coccoid bacteria, measuring 0.57 +/- 0.1 x 0.8 +/- 0.2 microm, that were Giemsa-positive, acid-fast-negative, and Gram-negative. The bacteria had a double cell wall, variable electron-dense and -lucent areas, and were present in the cytoplasm and within phagolysosomes. The syndrome was associated with cold stress and poor water conditions. These findings are consistent with an infectious process caused by a Piscirickettsia-like bacterium described previously in tilapia in Taiwan and Hawaii. This report involves the first identified cases of a piscirickettsiosis-like syndrome affecting tilapia in the continental United States.  相似文献   

A novel method for rearing the progeny of wild mediterranean fruit flies using artificial fruit     

Roy Kaspi  Idit Feitelson  Tamar Drezner  Boaz Yuval 《Phytoparasitica》2001,29(1):15-22

A technique for rearing the progeny of wildCeratitis capitata flies was developed. The method is based on wild captured flies which are allowed to oviposit in artificial fruit containing larval rearing medium. Flies develop in the fruit from eggs to last larval instar, then exit the fruit and pupate within vermiculite. This method was shown to be feasible and efficient, and to increase considerably the number of wild flies for testing purposes. Possible contribution to the Sterile Insect Technique (SIT) and potential applications of the method are discussed.  相似文献   

Effects of the halothane genotype and slaughter weight on texture of pork   总被引：4，自引：0，他引：4  

Monin G  Larzul C  Le Roy P  Culioli J  Mourot J  Rousset-Akrim S  Talmant A  Touraille C  Sellier P 《Journal of animal science》1999,77(2):408-415

The objective of this study was to investigate the effects of the halothane (HAL) genotype, slaughter weight (SW), and the HAL x SW interaction on compositional and textural traits of raw and cooked pork. Pigs were bred to exhibit one of the three HAL genotypes (NN, Nn, and nn) with otherwise equivalent genomes. The nn halothane reactors are known to typically produce PSE pork, whereas NN pigs do not typically produce PSE pork. Pietrain x Large White gilts and boars, all with verified Nn genotype (by DNA test), were mated to obtain F2 littermates of the three HAL genotypes. These pigs were slaughtered at either 101 +/- 3 ("light") or 127 +/- 3 ("heavy") kg BW and were evaluated for longissimus muscle traits. The pH at .5 h after death (pH1) was 6.35, 6.13, and 5.68 in NN, Nn, and nn pigs, respectively. Sarcomere length was greater in nn than in NN and Nn pigs (1.94 vs 1.83 and 1.85 microm, respectively). Mechanical resistance was higher in nn than in NN pigs for both raw and cooked meat. Meat from nn pigs was judged by a trained panel to be less rough, more cohesive, harder, more fibrous, less granular, more elastic, and less easy to swallow than meat from NN pigs. For most traits under study, the heterozygotes were intermediate between the homozygotes but closer to NN than to nn pigs. Muscle from heavy pigs had longer sarcomeres and less moisture than muscle from light pigs. The n allele of the HAL gene unfavorably affects pork texture, and this effect is maintained throughout the range of 101 to 127 kg BW.  相似文献   

Efficacy of an inactivated oil emulsion vaccine against hydropericardium syndrome in broilers     

Roy P  Koteeswaran A  Manickam R 《The Veterinary record》1999,145(16):458-459

Two inactivated vaccines were prepared against hydropericardium syndrome. The vaccine prepared from liver homogenate extracted with chloroform, inactivated with formalin and adjuvanted with liquid paraffin was highly effective against challenge in chickens aged three, five and seven weeks. Seroconversion following vaccination and challenge was assessed by the agar gel immunodiffusion test. The inactivated oil emulsion vaccine was highly effective against the syndrome in both experimental trials and field trials.  相似文献