Purification and Identification of Antioxidant Peptides from Fermented Fish Sauce (Budu)     

Leila Najafian  Abdul Salam Babji 《Journal Of Aquatic Food Product Technology》2019,28(1):14-24

This study aimed to isolate antioxidant peptides from Budu extract and identify novel antioxidant peptides using high-performance liquid chromatography (HPLC) and electrospray ionization-time-of-flight mass spectrometry (ESI-TOF MS/MS). An amino acid analyzer was used to analyze the amino acid composition of peptides. Two novel peptides, Lue-Asp-Asp-Pro-Val-Phe-Ile-His (LDDPVFIH) and Val-Ala-Ala-Gly-Arg-Thr-Asp-Ala-Gly-Val-His (VAAGRTDAGVH), were identified. The synthesized peptide of LDDPVFIH showed higher antioxidant activity. The presence of hydrophobic amino acids (Ile and Leu), acidic (Asp) and basic (His) amino acids in the peptide sequences is believed to contribute to the high antioxidant activity of the fermented anchovy fish (Budu) extract. Thus, the two peptides may have potential application as functional foods and could also be used as nutraceutical compounds.  相似文献   

Sodium alginate supplementation modulates gut microbiota,health parameters,growth performance and growth‐related gene expression in Malaysian Mahseer Tor tambroides     

Md Asaduzzaman  Shumpei Iehata  Md Moudud Islam  Md Abdul Kader  Abol‐Munafi Ambok Bolong  Daisuke Ikeda  Shigeharu Kinoshita 《Aquaculture Nutrition》2019,25(6):1300-1317

This study investigated the effects of sodium alginate supplementation on gut microbiota composition, health parameters, growth performances and growth‐related gene expression of Malaysian mahseer. Five test diets were formulated by supplementing 0%, 0.1%, 0.2%, 0.4% and 0.8% sodium alginate. Triplicate groups of juvenile Tor tambroides (2.19 ± 0.05 g) were stocked in 15 aquaria (20 individuals per aquarium) and fed at 3.0% body weight per day for 60 days. PCoA and UPGMA analysis showed that gut bacterial community were more convergence in higher sodium alginate‐supplemented diets. The percentage of Porphyromonadaceae, Bacteroides, Plesiomonas and Shewanella were substantially higher and Aeromonas, Entomoplasmatales and Prevotellaceae were drastically lower in higher sodium alginate (0.2%–0.8%) diets. Sodium alginate supplementation (≥0.2%) significantly improved the haematocrit value and respiratory burst activity of T. tambroides. Growth performances and feed utilization were significantly higher in 0.2%–0.4% sodium alginate‐supplemented diets. The increased growth rate of T. tambroides was governed by both hyperplastic and hypertrophic muscle growth. Real‐time PCR data demonstrated that most of the growth‐related genes were significantly upregulated in 0.2%–0.4% sodium alginate‐supplemented diet. Finally, it can be concluded that sodium alginate should be supplemented at 2 g/kg in practical fish feed formulation.  相似文献   

Antimycotic Screening of 58 Malaysian Plants against Plant Pathogens     

Suhaila Mohamed  Suzana Saka  Saleh H. El-Sharkawy  Abdul Manaf Ali  Sepiah Muid 《Pest management science》1996,47(3):259-264

Ethanolic extracts of 58 Malaysian plants belonging to 24 different families were screened for antifungal activity against seven plant pathogens using the filter paper disc diffusion technique. Two varieties of Piper betle, showed strong activity against all the pathogens tested (Colletotrichum capsici, Fusarium pallidoroseum, Botryodiplodia theobromae, Alternaria alternata, Penicillium citrinum, Phomopsis caricae-papayae and Aspergillus niger), with inhibition diameters significantly (P<0·01) bigger than 2·5 mg ml⁻¹ prochloraz or 10 mg ml⁻¹ clotrimazole. The minimum inhibitory concentrations of the ethanolic extracts of P. betle against these plant pathogens ranged between 0·01 mg ml⁻¹ and 1 mg ml⁻¹. Thirty-four other plants (Kucing gala, Limau batik, Bertholletia excelsa, Bixa orellana, Caesalpinia pulcherrima, Cerbera odollam (fruits and leaves), Colocasia gigantea, Curcuma domestica, Curcuma manga, Derris eliptica, Elephantopus scaber, Eleusine indica, Eugenia polyantha, Euphorbia hirta, Euphorbia tirucalli, Gardenia florida, Hedyotis auricularia, Hibiscus rosa-sinensis, Juniperus chinensis (three varieties), Lawsonia inermis, Lecythis ollaria, Mentha arvensis, Mimusops elengi, Ocimum sanctum, Phyllanthus niruri, Piper nigrum, Piperomia pellucida, Pedilanthus tithymaloides, Polygonum minus, Spondias dulcis, Solanum nigrum, Tinospora tuberculata) showed selective antifungal activity, while 21 species were inactive.  相似文献   

Irrigation and Zn fertilizer management improves Zn phyto‐availability in various rice production systems          下载免费PDF全文

Hafeez ur Rehman  Faiz Rasool  Masood Iqbal Awan  Athar Mahmood  Abdul Wakeel  Roghieh Hajiboland 《植物养料与土壤学杂志》2018,181(3):374-381

Zinc (Zn) is an important micronutrient for rice (Oryza sativa L.) production and its deficiency has been observed in various production systems. High grain Zn concentration is equally important for high rice yield and human health. In this work, the effects of Zn fertilization on seedling growth, grain yield, grain Zn concentration, and their association with root traits were studied under alternate wetting and drying (AWD), aerobic rice (AR), system of rice intensification (SRI), and continuous flooding (CF). Zinc fertilization (15 kg ha^?1) improved nursery seedlings chlorophyll and Zn concentrations, root length, and number of roots with highest values observed in CF. At harvesting, maximum plant height, panicle length, total and panicle bearing tillers, and kernel yield were found with Zn addition in AWD and CF rice systems. Mid season drainage provided at maximum tillering and Zn fertilization increased its concentration in leaves, culms, panicles, and grains under CF and AR at physiological maturity. Most of Zn applied was allocated into culms and panicles, nevertheless, a significant increase in grain Zn concentration was also observed in all production systems. Association of leaf Zn with grain Zn concentration was stronger than with culm and panicle Zn. The results indicate that Zn application after rice nursery transplanting is more important for grain Zn enrichment in all rice systems than for increase in grain yield in all systems except AWD where grain yield was also increased. More grain yield in CF and AWD as compared to SRI and AR can also be attributed to decreased spikelet sterility and to better Zn phyto‐availability in these rice systems at physiological maturity.  相似文献   

干旱区土壤螨类群落多样性及其与环境因子的相关性分析     

排孜丽耶·合力力  吾玛尔·阿布力孜  阿丽亚·司地克  阿迪拉古丽·阿布力克木 《新疆农业科学》2018,55(8):1483-1494

【目的】研究干旱区土壤环境因子对土壤螨类群落多样性的影响,对鄯善县7种不同生境进行土壤螨类群落调查与环境因子测定。【方法】采用改良的Tullgren法分离收集土壤螨类,并用Pearson相关分析和典范对应分析方法对土壤螨类群落多样性特征及其与环境因子之间进行相关分析。【结果】共捕获土壤螨类成体标本26 637只,隶属于4目69科98属。7种不同生境之间土壤螨类群落多样性指标均有极显著差异(P<0.001),其中Shannon-Wiener多样性指数(H)依次为防护林＞葡萄园＞杏园＞苜蓿地＞草地＞耕地＞菜地。CCA分析结果表明,第一主轴和第二主轴分别解释了土壤螨类主要群落总变量的29.7%和17.1%,与环境因子的相关性系数分别为0.926和0.929,前两轴共解释了土壤螨类物种类与环境因素关系的92.9%。蒙特卡罗置换检验显示,10个土壤主要理化性质与第一排序轴(F=4.31,P=0.002)和全部排序轴(F=2.68,P=0.002)均存在极显著的相关性。【结论】鄯善县7种不同生境土壤螨类群落多样性与土壤含水量、有机质、容重及全氮含量呈显著相关(P<0.05),其中土壤含水量、全氮和有机质含量对螨类群落结构和多样性的影响显著。  相似文献   

Influence of organic and inorganic passivators on Cd and Pb stabilization and microbial biomass in a contaminated paddy soil     

Saqib Bashir  Muhammad Shaaban  Qaiser Hussain  Sajid Mehmood  Jun Zhu  Qingling Fu  Omar Aziz  Hongqing Hu 《Journal of Soils and Sediments》2018,18(9):2948-2959

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Multilocus sequence analysis supports a low genetic diversity among ‘<Emphasis Type="Italic">Candidatus</Emphasis> Phytoplasma australasia’ related strains infecting vegetable crops and periwinkle in Egypt     

Yasmen El-Sisi  Ayman F. Omar  Samir A. Sidaros  Mohsen M. Elsharkawy  Xavier Foissac 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(3):779-784

‘Candidatus Phytoplasma australasia’ causes important damages to the Egyptian vegetable crop production. A prerequisite for controlling the different diseases it causes to eggplant, tomato and squash, is to trace its propagation pathways. To allow the differentiation of ‘Ca. P. australasia’ strains, a multilocus sequence analysis protocol was developed. Four conserved phytoplama genes namely tuf, secY, dnaK and dppA, were selected among the CDS of a ‘Ca. P. aurantifolia’ genome draft. The corresponding genes were PCR amplified from tomato, eggplant and squash collected in 2010 from the governorates Sharkia, Elmynia and Beni sueif, as well as from Catharanthus roseus periwinkles collected in 2013 from Kafrelsheikh governorate. Sequence comparisons showed no diversity among the Egyptian isolates of ‘Ca. P. australasia’ that also constitute a distinct cluster within the 16SrII-D taxonomic subgroup. This low diversity supports a common epidemiology for the different diseases affecting vegetable crops and periwinkle in Egypt and suggests that future investigations on insect vector should focus on polyphagous leafhoppers.  相似文献   

Preparation and Characterization of Microcrystalline Cellulose from Sacred Bali Bamboo as Reinforcing Filler in Seaweed-based Composite Film     

H. P. S. Abdul Khalil  Tze Kiat Lai  Ying Ying Tye  M. T. Paridah  M. R. Nurul Fazita  A. A. Azniwati  Rudi Dungani  Samsul Rizal 《Fibers and Polymers》2018,19(2):423-434

Microcrystalline cellulose (MCC) isolated from cheap, fast-growing and abundant accessible Sacred Bali bamboo (Schizostachyum brachycladum) was utilized as reinforcement material in the seaweed-based composite film. Isolation of MCC was carried out by using a combination of pulping, bleaching and acid hydrolysis process. This study emphasized on the feasibility of MCC production from Sacred Bali bamboo by studying its properties using X-ray diffraction (XRD), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The commercial-MCC (CMCC) was used as reference material. Results showed that the production yield, moisture content (MC) and fiber length of bamboo-MCC (BMCC) were 83.37±1.48 %, 4.50±0.5 %, and 0.47±0.02 mm, respectively. According to the chemical analysis by FTIR, both lignin and hemicellulose were completely removed from BMCC, and thus, improved the crystallinity (78 %) and thermal stability (≈325 °C) of BMCC. This study also revealed that MCC produced from Sacred Bali bamboo demonstrated strong mechanical reinforcement effect in the seaweed-based film. Hence, Sacred Bali bamboo-MCC could be used as reinforcement material in the polymer.  相似文献   

Influence of Hydrophobicity of Acetylated Nanocellulose on the Mechanical Performance of Nitrile Butadiene Rubber (NBR) Composites     

Mohamad Nurul Azman Mohammad Taib  Wageeh Abdulhadi Yehye  Nurhidayatullaili Muhd Julkapli  Sharifah Bee O. A. Abdul Hamid 《Fibers and Polymers》2018,19(2):383-392

Novel acetylation process by substitution of acetic anhydride substitution with hydroxyl groups on nanocellulose (NCC) has been explored to increase its dispersion and interaction in nitrile butadiene (NBR) matrix. The crystallinity index was increased after modification when compared to unmodified NCC, but no significant different with increases of treatment time from 1 hour to 3 hours treatments proven by X-ray diffraction (XRD) results. The Fourier transform infra-red (FTIR) showed the existent of acetyl groups on surface of the NCC after treatment by existent of the peak 1736 cm^-1 that attributed to carbonyl groups some peaks were observed at 1430, 1361 and 1248 cm^-1 and confirmed on the acetylation process. The nuclear magnetic resonance (NMR) also show the present of acetyl groups by existent of the signal of proton methyl group at 1.90 ppm and new peaks at 5.42, 4.70 and 4.34 ppm, for all ACN samples. The thermal results by thermo gravimetric analysis (TGA) and differential scanning calorimetry (DSC) showed that the acetylated NCCs were 10 % more thermally stable. Transmission electron microscopic (TEM) results showed that no significant changes were observed due to the acetylation process. The results also showed well distributed of individual NCC after acetylation, this improvement was primarily attributed to uniform dispersion of the ACN-NCC and less aggregated occurred. Due to its hydrophobic characteristics, highly crystalline and nano size, ACN-NCC brought a significant improvement up to 25 % on the mechanical properties of nitrile butadiene (NBR) rubber composites.  相似文献   

Recurrent parent genome recovery in different populations with the introgression of Sub1 gene from a cross between MR219 and Swarna-Sub1     

Fahim Ahmed  Mohd Y. Rafii  Mohd Razi Ismail  Abdul Shukor Juraimi  Harun Abdul Rahim  Fatah Abro Tanweer  Mohammad Abdul Latif 《Euphytica》2016,207(3):605-618

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