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21.
The present study describes the procedure for micropropagation of Dracocephalum kotschyi L. using shoot tips from in vitro-germinated plants. The best response was observed for shoot tips on MS medium containing 5 mg 6-benzylaminopurine L?1 and 0.2 mg 1-naphthaleneacetic L?1 acid. Regeneration for other types of the explant hypocotyls and cotyledons did not show satisfactory results so that the explants did not develop into normal shoots and in turn developed into the calli after 12 days of culture. Histochemical analysis showed that only the shoot tip revealed a direct induction of more teratological protuberances that arise around the cut end of the explants. Elongation of shoot buds was obtained on MS medium containing 1 mg BAP L?1 + 0.5 mg IBA L?1. Regenerated shoots rooted best on the same medium of elongation. After hardening, the rooted plants were transferred to the greenhouse where they grew, matured, and flowered normally with a survival rate of 95%. We concluded that the present protocol can be efficiently used for mass propagation of Dracocephalum kotschyi. 相似文献
22.
Development and evaluation of a loop-mediated isothermal amplification assay for detection of Erwinia amylovora based on chromosomal DNA 总被引：1，自引：0，他引：1
Aboubakr Moradi Jaber Nasiri Hamid Abdollahi Mohammadamin Almasi 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(3):609-620
A reliable and rapid pathogen detection protocol that utilizes loop-mediated isothermal amplification (LAMP) was developed for detection of Erwinia amylovora, the casual agent of fire blight. The six LAMP primers applied were derived from the highly conserved fragment of the chromosomally amsH gene. Despite the proposed LAMP as well as nested PCR presenting equal values of sensitivity (2?×?101?CFU/ml or more) for pure cultures, as compared with conventional PCR (2?×?103?CFU/ml), both methods were together superior. The specificity assay also showed that the LAMP protocol is species-specific for detection of E. amylovora even in inter-species analysis. Meanwhile, when all 208 naturally infected samples were examined, the specificity value of LAMP was 84%, while conventional and nested PCR could detect only 59% and 73% of the whole collection. Significantly, an independent behaviour versus host plant as well as each strain origin was also observed regarding the current LAMP method as well as other two PCR-based methods. All the results, overall, indicated that the LAMP offers an interesting novel and convenient assay format for the quick and specific chromosomal detection and diagnostic tool of recognition of E. amylovora and therefore presents an alternative to PCR-based assays. 相似文献
23.
Mahnaz Mohammadpour Hadi Samadian Nader Moradi Zhila Izadi Mahdieh Eftekhari Masoud Hamidi Amin Shavandi Anthony Quro Emmanuel Petit Cdric Delattre Redouan Elboutachfaiti 《Marine drugs》2022,20(1)
In the current paper, we fabricated, characterized, and applied nanocomposite hydrogel based on alginate (Alg) and nano-hydroxyapatite (nHA) loaded with phenolic purified extracts from the aerial part of Linum usitatissimum (LOH) as the bone tissue engineering scaffold. nHA was synthesized based on the wet chemical technique/precipitation reaction and incorporated into Alg hydrogel as the filler via physical cross-linking. The characterizations (SEM, DLS, and Zeta potential) revealed that the synthesized nHA possess a plate-like shape with nanometric dimensions. The fabricated nanocomposite has a porous architecture with interconnected pores. The average pore size was in the range of 100–200 µm and the porosity range of 80–90%. The LOH release measurement showed that about 90% of the loaded drug was released within 12 h followed by a sustained release over 48 h. The in vitro assessments showed that the nanocomposite possesses significant antioxidant activity promoting bone regeneration. The hemolysis induction measurement showed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability/proliferation confirmed the biocompatibility of the nanocomposites, which induced proliferative effects in a dose-dependent manner. This study revealed the fabricated nanocomposites are bioactive and osteoactive applicable for bone tissue engineering applications. 相似文献
24.
Mohamed Aboubakr Ahmed Soliman 《Journal of veterinary pharmacology and therapeutics》2020,43(5):512-515
Thiamphenicol (TP) pharmacokinetics were studied in Japanese quails (Coturnix japonica) following a single intravenous (IV) and oral (PO) administration at 30 mg/kg BW. Concentrations of TP were determined with HPLC and were analyzed by a noncompartmental method. After IV injection, elimination half-life (t1/2λz), total body clearance (Cltot) volume of distribution at steady state (Vdss), and mean residence time (MRT) of TP were 3.83 hr, 0.19 L/hr/kg, 0.84 L/kg, and 4.37 hr, respectively. After oral administration of TP, the peak plasma concentration (Cmax) was 19.81 μg/ml and was obtained at 2.00 hr (tmax) postadministration. Elimination half-life (t1/2λz) and mean absorption time (MAT) were 4.01 hr and 1.56 hr, respectively. The systemic bioavailability following oral administration of TP was 78.10%. TP therapy with an oral dosage of 30 mg/kg BW is suggested for a beneficial clinical effect in quails. 相似文献
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26.
Masoumeh Pouresmael Ramazan Ali Khavari-Nejad Javad Mozafari Farzaneh Najafi Foad Moradi 《Archives of Agronomy and Soil Science》2013,59(11):1561-1580
Drought is a worldwide concern and designation of drought stress adaptive mechanisms is one of the main directions in plant physiology and crop breeding. Genotypes diversity can be used to identify effective unexploited genes and pathways. In order to that, the effect of varying terminal drought intensity treatments on physiological and biochemical traits was evaluated in ILC3279, ICCV2 and FLIP9855 C chickpea lines. Well-watered, intermediate and severe drought treatments were applied from flowering till maturity. Photosynthetic efficiency, membrane stability, soluble sugar and proline content, leaf protein profile, and antioxidant enzyme activities were compared on 1st, 3rd, and 5th week after applying stress. Based on the results, it was found that the susceptibility of photosynthetic machinery of ILC3279 was more than others. Tolerant genotypes responded to drought differently; an increase and a decrease in catalase activity have been observed in ICCV2 and FLIP9855 C, respectively. The prominent role of soluble sugars was observed in ICCV2. Expressions of polypeptides 27 and 45 kDa in tolerant lines refer to their possible role in drought stress adaptation. Generally, in spite of significant variability in chickpea lines to cope with drought, lower ascorbate peroxidase activity, higher peroxidase activity, and higher Fv/Fm ratio can be tested as markers of chickpea drought tolerant. 相似文献
27.
The biodiversity of arbuscular mycorrhizal fungi (AMF) was surveyed in the Kolm region of Iran in three adjacent sites, a natural stand, a 10-year-old and a 15-year-old plantation of Amygdalus scoparia. To date, there have been few studies of AMF biodiversity in Iran, especially in the western forests of the country. For this study, soil and root samples were taken from A. scoparia rhizosphere soil in spring and autumn. Almost half of the root length was colonized by AMF. We identified 13 AMF species belonging to Glomeraceae, Claroideoglomeraceae or Diversisporaceae. The three plantations differed in terms of soil electrical conductivity, organic C and P. Spore density was significant correlated with P concentration. Root length colonization was correlated only with soil Ca. Species diversity and richness were significantly correlated with soil N, P, organic C and spore density. AMF diversity in 15-year-old plantations was more similar to that in the natural stand than in the 10-year-old plantation. We confirmed that a 15-year-old plantation is not similar in terms of AMF colonization to natural stands. We conclude that more than 15 years are required for AMF colonization of plantations to resemble that of natural stands. 相似文献
28.
K. Sorkheh B. Shiran M. Khodambashi H. Moradi T.M. Gradziel P. Martínez-Gómez 《Scientia Horticulturae》2010
Correlations between 29 quantitative tree and fruit traits were studied in 46 almond genotypes and some inter-specific crosses. Results reflected a significant diversity in the assayed almond germplasm. In addition, the obtained cluster demonstrated varying degrees of relationships, illustrating higher correlation values for the flowering traits and lower correlation values for the nut and kernel traits. To determine the importance of the traits and the levels of similarity existing between the various studied traits, a discriminate analysis was carried out. According to the results obtained from these analyses, the different traits were discriminated on basis of the characteristics of the presence of double kernels, the nut and the kernel size, the flowering date, kernel yield and shelling percentage. Finally, this study evidenced correlations between most of the agronomic traits in almond, although the correlations coefficient (r) value was found to be higher than 0.5 only in some cases. This analysis can help breeders for choosing the most favorable entries to build a core subset of the almond collection for the purpose of breeding. 相似文献
29.
Arababadi MK Hassanshahi G Yousefi H Zarandi ER Moradi M Mahmoodi M 《Pakistan journal of biological sciences: PJBS》2008,11(13):1738-1741
This research was aimed to investigate the prevalence and clinical impact of occult HBV infection in thalassemic patients with chronic HCV infection. In this cross-sectional study we have totally examined 60 patients suffering HBV and HCV infections by PCR and RT-PCR methods, respectively, in Kerman province of Iran. ELISA technique (RADIM, Italy) was used to detect anti-HBc, anti-HBs and HBsAg. The serum level of liver enzymes (SGOT, SGPT, DB, TB and ALK) were analyzed in the HCV infected patients (MAN, IRAN). Statistical analyses performed using t-test and Chi-square. We found that 27 cases (out of 60) were infected by HCV but HBV-DNA was not seen in HCV infected patients. Present findings also showed that none of samples were HBsAg positive but 9 (33%) (out of 27) HCV-RNA positive patients were anti-HBc positive and 11 (40.7%) were positive for anti-HBs. We found that SGOT, SGPT, DB, TB and ALK are above normal in 27 (100%), 19(70.3%), 12(44.5%), 15 (55.5%) and 15 (55.5%) RNA-HCV positive patients, respectively. The prevalence of hepatitis C infection is very high in thalassemic patients and based on other studies our results showed that the prevalence of HCV infection in Kerman is more than other provinces of Iran. In contrast with other studies HBV-DNA in these patients could not be detected, hence, it seems that occult HBV infection isn't frequent in Iranian thalassemic patients who suffering from chronic HCV infection. 相似文献
30.
Pharmacokinetics of cefquinome in red‐eared slider turtles (Trachemys scripta elegans) after single intravenous and intramuscular injections 
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下载免费PDF全文 K. Uney F. Altan G. Cetin M. Aboubakr B. Dik Z. Sayın A. Er M. Elmas 《Journal of veterinary pharmacology and therapeutics》2018,41(1):e40-e44
The purpose of this study was to evaluate the pharmacokinetics of cefquinome (CFQ ) following single intravenous (IV ) or intramuscular (IM ) injections of 2 mg/kg body weight in red‐eared slider turtles. Plasma concentrations of CFQ were determined by high‐performance liquid chromatography and analyzed using noncompartmental methods. The pharmacokinetic parameters following IV injection were as follows: elimination half‐life (t 1/2λz) 21.73 ± 4.95 hr, volume of distribution at steady‐state (V dss) 0.37 ± 0.11 L/kg, area under the plasma concentration–time curve (AUC 0–∞) 163 ± 32 μg hr?1 ml?1, and total body clearance (ClT) 12.66 ± 2.51 ml hr?1 kg?1. The pharmacokinetic parameters after IM injection were as follows: peak plasma concentration (C max) 3.94 ± 0.84 μg/ml, time to peak concentration (T max) 3 hr, t 1/2λz 26.90 ± 4.33 hr, and AUC 0–∞ 145 ± 48 μg hr?1 ml?1. The bioavailability after IM injection was 88%. Data suggest that CFQ has a favorable pharmacokinetic profile with a long half‐life and a high bioavailability in red‐eared slider turtles. Further studies are needed to establish a multiple dosage regimen and evaluate clinical efficacy. 相似文献