Modification of ocular defects in mouse developmental glaucoma models by tyrosinase     

Libby RT  Smith RS  Savinova OV  Zabaleta A  Martin JE  Gonzalez FJ  John SW 《Science (New York, N.Y.)》2003,299(5612):1578-1581

Mutations in the cytochrome P450 family 1, subfamily B, polypeptide 1 (CYP1B1) gene are a common cause of human primary congenital glaucoma (PCG). Here we show that Cyp1b1-/- mice have ocular drainage structure abnormalities resembling those reported in human PCG patients. Using Cyp1b1-/- mice, we identified the tyrosinase gene (Tyr) as a modifier of the drainage structure phenotype, with Tyr deficiency increasing the magnitude of dysgenesis. The severe dysgenesis in eyes lacking both CYP1B1 and TYR was alleviated by administration of the tyrosinase product dihydroxyphenylalanine (l-dopa). Tyr also modified the drainage structure dysgenesis in mice with a mutant Foxc1 gene, which is also involved in PCG. These experiments raise the possibility that a tyrosinase/l-dopa pathway modifies human PCG, which could open new therapeutic avenues.  相似文献   

Production of phytoalexins, glycoalkaloids and phenolics in leaves and tubers of potato cultivars with different degrees of field resistance after infection withPhytophthora infestans     

Adriana Andreu  Claudia Oliva  Sonia Distel  G. Daleo 《Potato Research》2001,44(1):1-9

Summary The kinetics of accumulation of phytoalexins, glycoalkaloids and phenolics was studied in two potato cultivars differing in their degrees of field resistance when infected withPhytophthora infestans. Tuber slices and leaves of cvs Pampeana INTA (high degree of field resistance, free of R genes) and Bintje (susceptible) were infected with race C (complex race 1, 3, 5, 7, 11) ofPhytophthora infestans. Phytoalexins and phenolics accumulated in tuber and leaf tissues which had been inoculated. The levels of these compounds in the susceptible cv. Bintje were relatively low and similar to those found before inoculation. Leaves of cv. Pampeana INTA had a very high glycoalkaloid content, suggesting that glycoalkaloids may play a role in protection of leaves against the fungus. However, we could find no correlation between resistance and glycoalkaloid content of tubers. Our results suggest a major role of phytoalexins, phenolics and glycoalkaloids in the complex mechanisms of field resistance.  相似文献   

Effect of density on survival,physiological parameters and water quality during pirarucu transportation in open system          下载免费PDF全文

Adriana Ferreira Lima  Hyago Jovane Borges de Oliveira 《Aquaculture Research》2018,49(2):947-952

The loading density of pirarucu Arapaima gigas transported for 6 hr in open system was assessed on survival, physiological parameters (blood hematocrit, cortisol, lactate and glucose concentrations) and water quality (temperature, pH, ammonia, dissolved oxygen and carbon dioxide). No mortality was observed after fish transportation at 80, 120 and 160 kg/m³, although ammonia and carbon dioxide concentrations increased and dissolved oxygen decreased. Physiological parameters were not affected by loading density but significant differences were observed between sampling periods (immediately before transport, immediately after transport, 24 and 96 hr after transport), except glucose. Hematocrit values increased with time, whereas lactate concentration decreased. No significant differences were observed in blood cortisol levels before and after transport, but concentration increased gradually for 96 hr after transport. According to the results, 9 kg pirarucu may be transported for up to 6 hr at a loading density of 160 kg/m³.  相似文献   

Natural food intake by juvenile Arapaima gigas during the grow‐out phase in earthen ponds          下载免费PDF全文

Adriana Ferrreira Lima  Giovanni Vitti Moro 《Aquaculture Research》2018,49(5):2051-2058

In carnivorous fish species, zooplankton is one of the main food items in the early life stages and some fish species continue feeding on such food items further along the life stages even in the farming environment. In this study, the intake of natural food items was assessed in juvenile pirarucu Arapaima gigas reared in earthen ponds. Juvenile pirarucu (12.2 ± 4.32 g and 12.1 ± 1.13 cm) were stocked in fertilized earthen ponds (240 m²). For the analysis of the fish stomach content and plankton in the pond water, the fish and pond water were sampled weekly for 75 days and biweekly until the fish reached a mean weight of 750 g. Although artificial feed was used, pirarucu also ingested the natural food available in the pond water. Among the zooplankton, pirarucu demonstrated feeding preference for cladocerans despite the abundance of rotifers and copepods. Cladocerans were present in more than 80% of the stomach contents of fish up to 300 g and in 65%, 45% and 17% of fish of 301–500 g, 501–700 g and 701–900 g respectively. Copepods were present only in fish up to 500 g at low abundance. High ingestion of insects and plant material was observed in the stomach content of fish of all size classes. The results demonstrate that juvenile pirarucu ingest natural food available in the farming pond and suggests that the adoption of pond fertilization practices may have positive effects on fish growth performance.  相似文献   

Diallel analysis for morphoagronomic descriptors in Physalis angulata L. hybrids     

de Souza Farias  Jos&#; Williano  Orella  Jonathan Said Tejeda  dos Santos Batista  Edgar  Cordeiro  Rafael Cruz  Passos  Adriana Rodrigues  Silva  Luiz Cl&#;udio Costa 《Genetic Resources and Crop Evolution》2022,69(3):1365-1374

Genetic Resources and Crop Evolution - Physalis angulata L. is an American species with edible fruits that stands out for having high nutritional and pharmaceutical value. The species is found in...  相似文献