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91.
In order to extend scientific knowledge on autochthonous Italian equine meat, the physical–chemical parameters of Catria Horse Longissimus thoracis (LT) muscle and its nutritional characteristics have been investigated. Ten steaks of Catria foal raised at pasture and fattened indoors for 2 months were dissected, and LT muscle was analyzed for chemical composition, total iron, drip loss, colorimetric characteristics, intramuscular fat, fatty acid profile and nutritional indexes. Steak dissection showed that LT muscle accounted for 36.78% and fat accounted for 9.19% of weight of steak. Regarding chemical composition, protein and fat content was 20.31% and 2.83%, respectively. Total iron content (1.95 mg/100 g) was lower than data reported in the literature. Color parameters showed a luminous and intense red hue muscle. The sum of unsaturated fatty acid composition (50.3%) was higher than the sum of saturated fatty acids (46.64 %). The fatty acid profile and nutritional values of Catria Horse meat could be modified adopting extensive rearing systems and grazing. The data suggests that further investigation on the composition of Catria Horse meat should be carried out to valorize this autochthonous breed, reared in sustainable livestock systems, and its meat in local short‐chain systems.  相似文献   
92.

Purpose

The objective of this research is to find out the spatial distribution of different traffic-related contaminants in urban topsoils across a highway (125,000 vehicles per day) in the city of Moscow, Russia.

Materials and methods

The topsoils (0–3 cm depth) were sampled 1, 6, 10, 18, and 50 m perpendicular to the roadbed in three replicates 10 m apart. We analyzed total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAH), heavy metals in total and phyto-available forms (HM; Cu, Zn, and Pb), and deicing salts (DS; Cl?). The TPH were extracted with carbon tetrachloride and detected by spectrometry and PAH were extracted with methylene chloride, separated by high-performance liquid chromatography, and determined by fluorescence detector. Total HM were extracted by microwave acid digestion with aqua regia and phyto-available forms were extracted by NH4OAc and determined by inductively coupled plasma mass spectrometry. The DS were analyzed using an ion liquid chromatograph with a conductometric detector.

Results and discussion

Heavy soil pollution was found within 1–6 m of the road. The TPH, B[a]P, and phyto-available HM concentrations exceeded permissible levels for Russia, while total HM and DS did not. The contaminant contents sharply decreased 10 m from the road. Within 10–50 m, they reached background levels, while PAH levels were elevated. We attribute this to the greater age of soils at 10–50 m than 1–6 m from the road. The different contaminant distribution patterns are attributed to their different transportation pathways: TPH, DS, and HM generally reach soils through road spray, splashes, melting snow moved from the road surface to roadsides, and aerosol sedimentation, whereas PAH are mainly transported as airborne particles and are thus able to reach more distant locations.

Conclusions

Traffic-related soil pollution creates ecotoxicological and human-health risks. Distribution patterns vary by type of soil contaminant. The influence of the road on soil contamination was greatest within 10 m from the roadbed for TPH, HM, and DS and within 50 m for PAH. The increased PAH concentration levels with increased distance from the road highlights the importance of PAH monitoring in roadside soils.
  相似文献   
93.
Winfried Schröder  Stefan Nickel  Simon Schönrock  Roman Schmalfuß  Werner Wosniok  Michaela Meyer  Harry Harmens  Marina V. Frontasyeva  Renate Alber  Julia Aleksiayenak  Lambe Barandovski  Oleg Blum  Alejo Carballeira  Maria Dam  Helena Danielsson  Ludwig De Temmermann  Anatoly M. Dunaev  Barbara Godzik  Katrin Hoydal  Zvonka Jeran  Gunilla Pihl Karlsson  Pranvera Lazo  Sebastien Leblond  Jussi Lindroos  Siiri Liiv  Sigurður H. Magnússon  Blanka Mankovska  Encarnación Núñez-Olivera  Juha Piispanen  Jarmo Poikolainen  Ion V. Popescu  Flora Qarri  Jesus Miguel Santamaria  Mitja Skudnik  Zdravko Špirić  Trajce Stafilov  Eiliv Steinnes  Claudia Stihi  Ivan Suchara  Lotti Thöni  Hilde Thelle Uggerud  Harald G. Zechmeister 《Annals of Forest Science》2017,74(2):31

Key message

Moss surveys provide spatially dense data on environmental concentrations of heavy metals and nitrogen which, together with other biomonitoring and modelling data, can be used for indicating deposition to terrestrial ecosystems and related effects across time and areas of different spatial extension.

Context

For enhancing the spatial resolution of measuring and mapping atmospheric deposition by technical devices and by modelling, moss is used complementarily as bio-monitor.

Aims

This paper investigated whether nitrogen and heavy metal concentrations derived by biomonitoring of atmospheric deposition are statistically meaningful in terms of compliance with minimum sample size across several spatial levels (objective 1), whether this is also true in terms of geostatistical criteria such as spatial auto-correlation and, by this, estimated values for unsampled locations (objective 2) and whether moss indicates atmospheric deposition in a similar way as modelled deposition, tree foliage and natural surface soil at the European and country level, and whether they indicate site-specific variance due to canopy drip (objective 3).

Methods

Data from modelling and biomonitoring atmospheric deposition were statistically analysed by means of minimum sample size calculation, by geostatistics as well as by bivariate correlation analyses and by multivariate correlation analyses using the Classification and Regression Tree approach and the Random Forests method.

Results

It was found that the compliance of measurements with the minimum sample size varies by spatial scale and element measured. For unsampled locations, estimation could be derived. Statistically significant correlations between concentrations of heavy metals and nitrogen in moss and modelled atmospheric deposition, and concentrations in leaves, needles and soil were found. Significant influence of canopy drip on nitrogen concentration in moss was proven.

Conclusion

Moss surveys should complement modelled atmospheric deposition data as well as other biomonitoring approaches and offer a great potential for various terrestrial monitoring programmes dealing with exposure and effects.
  相似文献   
94.
Up to 2005 the sensitivity of Stemphylium vesicarium (Wallr.) Simm., the causal agent of pear brown spot, to the strobilurin fungicides kresoxim-methyl, trifloxystrobin and pyraclostrobin was still comparable with baseline values associated with good efficacy in the field. During 2006, the first resistant isolates were detected in two commercial pear orchards in the Emilia-Romagna region (Italy), one of which was affected by considerable control failure linked to strobilurin treatments as demonstrated in a field trial. In vitro sensitivity tests with 0.5 mg l−1 of kresoxim-methyl, trifloxystrobin and pyraclostrobin showed that in the population collected in the orchard with control failure the conidial germination was greater than 90% compared to an untreated control both in 2006 and in 2007, i.e. 1 year after the suspension of strobilurin applications. In the other orchard, where only a few symptomatic fruits were found and the strobilurins were still in use, the conidial germination was lower, about 50% in 2006 and 25% in 2007. The molecular analysis of mitochondrial cytochrome b gene of some monospore isolates with different levels of sensitivity confirmed the presence of the mutation causing G143A substitution in all the resistant isolates. In conclusion, both in vitro tests and molecular analysis confirmed the first occurrence of Stemphylium vesicarium resistance to all strobilurin fungicides tested.  相似文献   
95.
In order to compare the efficacies of new rodenticides (sodium selenite and cellulose) and conventional ones (bromadiolone, brodifacoum and cholecalciferol) in controlling Microtus arvalis (Pallas, 1778) in wheat and alfalfa crops, experiments were conducted at two sites near Belgrade, Serbia, in the spring of 2008, using a standard EPPO method. The presence of rodent populations, their spatial distribution and density indexes were evaluated by pre-treatment census, and rodenticide efficacy by counting active holes 14 and 28 days after treatment.  相似文献   
96.
Porcine circoviruses (PCV) are widespread in domestic pigs worldwide and there is growing information about the presence of PCV in other suid species. Based on serological studies with sera of wild boars, it was established that PCV1 was present in these animals and antibodies specific to PCV2 were also detected in wild boars living in captivity or in sylvatic areas, both with or without clinical signs of PMWS. Studies including PCV2 genome or antigen detection confirmed the previous findings. This is the first report about the presence of PCV in Transylvanian wild boar populations. Four hundred and sixty-nine samples were collected and grouped according to geographic origin, tested for the presence of PCV DNA using a real-time quantitative polymerase chain reaction assay, and 13.52% of the animals proved to be positive for one or in three cases both of the PCV genotypes. PCV2 was detected in all of the PCV-positive samples.  相似文献   
97.
The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases, and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had an efficiency of 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production.  相似文献   
98.
The production of new bioactive oligosaccharides is currently garnering much attention for their potential use as functional ingredients. This work addresses the enzymatic synthesis and NMR structural characterization of 2-α-D-glucopyranosyl-lactose derived from sucrose:lactose and sucrose:cheese whey permeate mixtures by using a Leuconostoc mesenteroides B-512F dextransucrase. The effect of synthesis conditions, including concentration of substrates, molar ratio of donor/acceptor, enzyme concentration, reaction time, and temperature, on the formation of transfer products is evaluated. Results indicated that cheese whey permeate is a suitable material for the synthesis of 2-α-D-glucopyranosyl-lactose, giving rise to yields around 50% (in weight respect to the initial amount of lactose) under the optimum reaction conditions. According to its structure, this trisaccharide is an excellent candidate for a new prebiotic ingredient, due to the reported high resistance of α-(1→2) linkages to the digestive enzymes in humans and animals, as well as to its potential selective stimulation of beneficial bacteria in the large intestine mainly attributed to the two linked glucose units located at the reducing end that reflects the disaccharide kojibiose (2-α-D-glucopyranosyl-D-glucose). These findings could contribute to broadening the use of important agricultural raw materials, such as sucrose or cheese whey permeates, as renewable substrates for enzymatic synthesis of oligosaccharides of nutritional interest.  相似文献   
99.
The objective of this study is to evaluating the Brazilian biodiversity through physicochemical characterization and determination of antioxidant potential of three species from the Myrtaceae family, namely yellow guava (Psidium cattleyanum Sabine), guabiroba (Campomanesia xanthocarpa O. Berg), and uvaia ( Eugenia pyriformis Cambess). Guabiroba had the greater quantity of phenolic compounds (9033 mg chlorogenic acid/100 g) and vitamin C (30.58 mg/g) and showed the best TSS/TTA (total soluble solid/total titratable acid) ratio (45.12). For the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic) method, the guabiroba (507.49 μM Trolox/g) presented the highest antioxidant potential; however, in the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, uvaia (170.26 g/g DPPH) and guabiroba (161.29 g/g DPPH) were not statistically different. The uvaia outranked the other fruits with respect to its high carotenoid (909.33 μg/g) and vitamin A (37.83 μg/g) contents, and the yellow guava, although showing a lower bioactive compound content and antioxidant activity, nevertheless presented much higher values than many traditionally consumed fruits.  相似文献   
100.
This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with carob germ flour.  相似文献   
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