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991.
The demographic structure of populations is affected by life history strategies and how these interact with natural and anthropogenic factors such as exploitation, climate change, and biotic interactions. Previous work suggests that the mean size and age of some North American populations of Chinook salmon (Oncorhynchus tshawytscha, Salmonidae) are declining. These trends are of concern because Chinook salmon are highly valued commercially for their exceptional size and because the loss of the largest and oldest individuals may lead to reduced population productivity. Using long‐term data from wild and hatchery populations, we quantified changes in the demographic structure of Chinook salmon populations over the past four decades across the Northeast Pacific Ocean, from California through western Alaska. Our results show that wild and hatchery fish are becoming smaller and younger throughout most of the Pacific coast. Proportions of older age classes have decreased over time in most regions. Simultaneously, the length‐at‐age of older fish has declined while the length‐at‐age of younger fish has typically increased. However, negative size trends of older ages were weak or non‐existent at the southern end of the range. While it remains to be explored whether these trends are caused by changes in climate, fishing practices or species interactions such as predation, our qualitative review of the potential causes of demographic change suggests that selective removal of large fish has likely contributed to the apparent widespread declines in average body sizes.  相似文献   
992.
Ascorbic acid (AA) is an antioxidant considered to play a crucial role in human health. Therefore, diverse methods for the determination of AA in foods have been developed, most of them time-consuming and requiring costly instrumentation. A simple and sensitive method for the quantification of AA in fresh fruits and vegetables and commercial juices using an amperometric sensor is presented on the basis of disposable screen-printed carbon electrodes (SPEs) modified with an o-aminophenol (o-AP) film selective for the detection of AA. The sensor exhibited a linear response for AA from 2-20 microM, with a correlation coefficient r2 = 0.998 and a limit of detection of 0.86 microM. Common possible interferents of the sample matrices were tested, and results showed high selectivity of the o-AP SPEs toward AA. The sensor exhibited an excellent reproducibility (RSD% = 1.98, n = 8) and surface stability. The method was validated by a comparison to a reference method, and excellent correlation is obtained.  相似文献   
993.
African oil palm (Elaeis guineensis Jacq.) is considered the most productive oleaginous crop due to its high oil production per hectare. In April 2015, E. guineensis leaves showing elliptical necrotic spots with yellowish halo were observed in a commercial plantation in Moju, Pará state, Brazil. A synematous fungus was consistently observed associated with the necrotic spots. The aim of this study was to identify this fungus associated with E. guineensis in Brazil. Based on morphology and DNA sequence data of the internal transcribed spacer rDNA, the fungus was identified as Helminthosporiella stilbacea. Helmintosporiella stilbacea has been reported associated with leaf spots on E. guineensis in Africa, Ghana, Sudan and Zambia. To the best of our knowledge, this is the first report of H. stilbacea associated with E. guineensis in Brazil.  相似文献   
994.
Erratic rainfall is often a limiting factor in the semi-arid regions where most groundnut cultivation occurs. As a result, ensuring availability of cultivars that possess inherent tolerance to drought stress has become a priority. Field and box (wooden boxes of 2 m length × 1 m width × 0.3 m depth) experiments were conducted under drought and non-drought conditions to identify physiological and agronomic traits correlated with pod yield (PY). Fifty (50) advanced breeding lines were evaluated. Linear models containing different combinations of total dry matter at maturity, crop growth rate, pod growth rate, partition coefficient, and harvest index were able to predict PY under intermittent drought (adjusted R2 range: 0.9798–0.9895). The box experiment was more discriminating of genotypes than field experiments, making it a suitable technique for drought tolerance screening using specific leaf area and leaf chlorophyll content. As a result, screening and pre-selection using the seed-box technique before advanced evaluation on the field is recommended.  相似文献   
995.
996.
Challenge of naive experimental animals with a retroviral inoculum may result in one of two broad sequelae. The first is the establishment of an appropriate humoral and cellular immune response leading to a condition of immunity to subsequent infection with the retrovirus. Alternatively, the host may fail to develop a successful immune response, resulting in a chronic viremia associated with immunosuppression and ultimately death due to secondary pathogens. An alternate disease course is the establishment of a latent infection characterized by the presence of neutralizing antibody and strong cellular immune reactivity. Recent data from the feline leukemia virus (FeLV) system suggest that cats infected with this virus may develop immunosuppression in the form of persistent neutrophil dysfunction. The potential effect of this cellular dysfunction is the possible susceptibility of the host to the same opportunistic pathogens which are responsible for the increased mortality noted in chronic FeLV infections. These data demonstrate that persistent retroviremia is not essential for the establishment of immunosuppression. This overview presents data accumulated from the feline model of the human acquired immunodeficiency syndrome (AIDS) and discusses its relationship to human retroviral infections.  相似文献   
997.
998.
Effectiveness of vaccination in controlling ovine footrot   总被引:2,自引:0,他引:2  
Efficacy of reducing footrot by use of a commercial vaccine was evaluated over 2 yr with 223 ewes from five crossbred genotypes. Ewes were assigned randomly within genotype, age and initial footrot status to a 2 x 3 factorial array of vaccination in the 1st yr and fall vaccination, winter vaccination or control in the 2nd yr. The flock contained a single Bacteroides nodosus serotype (XV) known to be highly cross-reactive with one of the vaccine serotypes (British serotype H). Vaccination reduced footrot incidence by 61% (P less than .01) in the 1st yr and by 45% (P less than .01) in the 2nd yr. Vaccination of previously uninfected ewes did not significantly reduce the rate of new infection, but vaccination of infected ewes reduced re-infection by 92% (P less than .01). Vaccination in the 1st yr failed to produce any carryover or additive protection in the 2nd yr. Genotypes differed in both footrot incidence (P less than .05) and footrot reduction following vaccination (P less than .05), but the effect was inconsistent between years. Vaccination increased serum antibody titers in both years (P less than .01). Among vaccinates in the 1st yr, infected ewes had lower titer levels than uninfected ewes (P less than .01), but no correlation between titer level and incidence was observed in the 2nd yr.  相似文献   
999.
Pemphigus antigens were localized, by use fo immunoelectron microscopy, on canine keratinocytes in vivo on esophageal mucosa and in vitro on established cultured keratinocytes. Convalescent sera from a human being with pemphigus vulgaris and a human being with pemphigus foliaceus reacted with the interdesmosomal cytoplasmic keratinocyte membrane of canine esophagus. Cultured canine keratinocytes expressed the pemphigus vulgaris antigen in a similar pattern, but did not carry the pemphigus foliaceus antigen. The differential presence of cell surface antigens and its relation to various forms of the disease are discussed.  相似文献   
1000.
A trial was conducted to examine the potential of using plasma amino acid responses to graded levels of escape protein to determine limiting amino acids in cattle. Growing calves (n = 120; mean BW = 220 +/- 21 kg) were fed a basal diet of corncob:sorghum silage (61:39) and were individually supplemented with distillers' dried grains (DDG), heat-damaged DDG (H-DDG), feather meal (FTH), or urea. The urea supplement was mixed with DDG and H-DDG to allow 0, 20, 35, 50, 65, or 80% of the supplemental CP to come from distillers' protein and maintain an 11.5% CP diet. Urea supplement was mixed with FTH to allow 0, 22, 39, 56, 73, or 90% of the supplemental CP to come from FTH. Dietary CP ranged from 11.5% at the 0% level to 17.3% at the 90% level. Plasma concentration of most essential plasma amino acids responded (P less than .10) linearly and(or) quadratically to increased escape protein. The broken-line response of plasma methionine at low DDG intake suggested that methionine was limiting at low levels of escape protein. An initial decrease followed by a plateau fit by a broken line indicated that histidine became limiting in FTH diets, and lysine eventually became limiting for DDG, H-DDG, and FTH diets before maximum BW gain was reached. Results indicate that plasma amino acid responses may identify amino acids that become limiting with increasing escape protein.  相似文献   
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