Cytotoxic Marine Alkaloid 3,10-Dibromofascaplysin Induces Apoptosis and Synergizes with Cytarabine Resulting in Leukemia Cell Death     

Pavel Spirin  Elena Shyrokova  Timofey Lebedev  Elmira Vagapova  Polina Smirnova  Alexey Kantemirov  Sergey A. Dyshlovoy  Gunhild von Amsberg  Maxim Zhidkov  Vladimir Prassolov 《Marine drugs》2021,19(9)

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Traditional taxonomy: Quo vadis?          下载免费PDF全文

Alexey A. KOTOV  Maria A. GOLOLOBOVA 《Integrative zoology》2016,11(6):500-505

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An aegialodontid upper molar and the evolution of mammal dentition     

Lopatin AV  Averianov AO 《Science (New York, N.Y.)》2006,313(5790):1092

The most obvious key synapomorphy of the therian mammals is the tribosphenic pattern of their molars. Tribosphenic teeth are capable of both shearing and grinding, which substantially increase effectiveness of food processing and, in turn, permit evolution of a wide range of dietary specializations. Functional tribospheny developed repeatedly during mammalian evolution but was successful only in the Boreosphenida. The earliest stage in the development of boreosphenidan tribospheny has remained poorly understood, being documented only by lower molars of aegialodontids. Here, we report a known upper molar of an aegialodontid mammal, Kielantherium, from the Early Cretaceous of Mongolia.  相似文献   

Chromosomes can congress to the metaphase plate before biorientation     

Kapoor TM  Lampson MA  Hergert P  Cameron L  Cimini D  Salmon ED  McEwen BF  Khodjakov A 《Science (New York, N.Y.)》2006,311(5759):388-391

The stable propagation of genetic material during cell division depends on the congression of chromosomes to the spindle equator before the cell initiates anaphase. It is generally assumed that congression requires that chromosomes are connected to the opposite poles of the bipolar spindle ("bioriented"). In mammalian cells, we found that chromosomes can congress before becoming bioriented. By combining the use of reversible chemical inhibitors, live-cell light microscopy, and correlative electron microscopy, we found that monooriented chromosomes could glide toward the spindle equator alongside kinetochore fibers attached to other already bioriented chromosomes. This congression mechanism depended on the kinetochore-associated, plus end-directed microtubule motor CENP-E (kinesin-7).  相似文献   

Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A     

da Cunha IA  Zulpo DL  Bogado AL  de Barros LD  Taroda A  Igarashi M  Navarro IT  Garcia JL 《Veterinary parasitology》2012,186(3-4):216-221

We evaluated the humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A. The experiment used 13 mixed-breed pigs divided into the following three groups: G1 (vaccinated-challenged, n=6), which received the rhoptry vaccine (200(g/dose); G2 (adjuvant-challenged, n=4), which received PBS plus Quil-A; and G3 (unvaccinated-challenged, n=3), which was the control group. The treatments were performed intranasally at days 0, 21, and 42. Three pigs from G1 produced IgG and IgM antibody levels above the cut-off in the ELISA on the challenge day. Partial protection was observed in G1 at the chronic phase of infection when compared with G3. The preventable fractions were 41.6% and 6.5%, in G1 and G2, respectively. The results of this study suggest that rhoptry proteins plus Quil-A stimulated humoral, local, and systemic immune responses, which were able to partially protect the brain from cyst formation.  相似文献   

Biochemistry. DNA building block reinvented     

Murzin AG 《Science (New York, N.Y.)》2002,297(5578):61-62

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肉类加工机刀具制造与修复的新方法     

Rudt.  FY Gut.  MSH 《华中农业大学学报》2000,19(3):292-295

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Comment on "A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint"     

Taylor SS  Hardwick KG  Sawin KE  Biggins S  Piatti S  Khodjakov A  Rieder CL  Salmon ED  Musacchio A 《Science (New York, N.Y.)》2007,316(5827):982; author reply 982

Müller et al. (Reports, 27 October 2006, p. 654) proposed a role for microtubule nucleation in mitotic checkpoint signaling. However, their observations of spindle defects and mitotic delay after depletion of gamma-tubulin ring complex (gamma-TuRC) components are fully consistent with activation of the established pathway of checkpoint signaling in response to incomplete or unstable interactions between kinetochores of mitotic chromosomes and spindle microtubules.  相似文献   

Natural variations of precursors in pig meat affect the yield of heterocyclic amines--effects of RN genotype, feeding regime, and sex   总被引：3，自引：0，他引：3  

Olsson V  Solyakov A  Skog K  Lundström K  Jägerstad M 《Journal of agricultural and food chemistry》2002,50(10):2962-2969

Pig meat shows natural variations in the concentrations of precursors of heterocyclic amines (HCAs), which may affect formation of HCAs in cooked pig meat. To study this, 26 pigs with an inherent genetic variation (carriers and noncarriers of the RN(-) allele) were subjected to different feeding regimes (conventional feed compared with feed composed according to organic standards). In addition, the effect of sex (castrated males or females) was considered when assessing chemical and technological meat quality parameters. Concentrations of precursors of HCAs, i.e., creatine, residual glycogen, dipeptides, and free amino acids, were analyzed in the raw meat, and the levels of some HCAs (4,8-DiMeIQx, MeIQx, PhIP, harman, and norharman) were then determined in fried meat patties prepared from these pigs. The RN genotype most affected technological meat quality parameters and the level of precursors of HCAs, especially the level of residual glycogen, where carriers of the RN(-) allele showed levels four times as high as those of noncarriers (75.3 +/- 2.6 compared with 17.2 +/- 2.4 micromol/g meat, least-squares means +/- SE). The increased level of residual glycogen resulted in about 50% lower amounts of total mutagenic HCAs in cooked meat compared with cooked meat from normal pigs. Fried meat from carriers of the RN(-) allele obtained darker crust color than meat from noncarriers. Feeding regime and sex did not significantly affect the chemical composition of the meat or the formation of HCAs.  相似文献   

Impact of agricultural land use on distribution of microbial biomass and activity within soil aggregates     

Anastasia V. Vasilchenko  Lyudmila V. Galaktionova  Nikolay Yu. Tretyakov  Sergey M. Dyachkov  Alexey S. Vasilchenko 《Soil Use and Management》2023,39(1):618-633

The presence of aggregates of various sizes in the soil is an important condition for soil carbon sequestration. In this system, microbial biomass is a key link. This work was devoted to the study of the influence of land use systems on the distribution of SOС, MB-SIR, microbial activity and eco-physiological indices (qCO₂, QR, MB-SIR/SOС and qCO₂/SOС) in relation to the size of soil aggregates. The distribution of SOС, MB-SIR and mineralization activity among the aggregates was heterogeneous. In the soil of crop rotation, high mineralization activity and MB-SIR were found in the aggregates 0.5–0.1 mm, in the monoculture soil in aggregates <0.1 mm and in the control soil in the aggregates 1–0.25 mm. There was a general trend towards a decrease in microbial activity, MB-SIR and SOС availability with an increase in aggregate size. In agricultural soils, microbial activity was determined by large aggregates (>5 mm), while in the control soil, by the aggregates 5–1 mm. Depending on the type of site and the size of aggregates, the differences in microbial metabolism were revealed. The qCO₂ and QR values decreased, and the MB-SIR/SOС and qCO₂/SOС increased in the series: control soil > crop rotation > monoculture. In the control soil, the values of the eco-physiological indices decreased with decreasing aggregate size. And vice versa, in agricultural soils, these parameters were the highest in the microaggregates (<0.25 mm). The monoculture soil, in contrast to the control soil and crop rotation soil, turned out to be more energy efficient.  相似文献