Hematology of free-living marsh deer (Blastocerus dichotomus) from southeast Brazil.     

Matias Pablo Juan Szabó  Eliana Reiko Matushima  Márcio Botelho de Castro  Danilo Alvaro Santana  Cátia Dejuste de Paula  José Maurício Barbanti Duarte 《Journal of zoo and wildlife medicine》2005,36(3):463-469

This work reports basic hematologic values of a sample of a population of free-living marsh deer (Blastocerus dichotomus) living by the Paraná River in Southeast Brazil. Hematologic values are presented separately for male, female, and young animals as well as for anesthetized and nonanesthetized cervids. Nonanesthetized deer restrained by physical means had significantly higher erythrocyte indices and total leukocyte counts. Comparisons of blood parameters of anesthetized animals of different ages and gender differed slightly, with only two significant differences observed: young animals had significantly higher red blood cell counts than adult males and a lower blood total protein content when compared to adult females. Results indicate that two main reference ranges for blood values should be considered for marsh deer, for blood obtained from anesthetized or physically restrained individuals.  相似文献   

Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies     

Michalsky EM  Rocha MF  da Rocha Lima AC  França-Silva JC  Pires MQ  Oliveira FS  Pacheco RS  dos Santos SL  Barata RA  Romanha AJ  Fortes-Dias CL  Dias ES 《Veterinary parasitology》2007,147(1-2):67-76

Visceral leishmaniasis (VL) is a growing zoonosis with an increasing number of new cases and a rapid geographical spreading of the disease. In the present study, a canine survey was carried out in the city of Montes Claros (320,000 inhabitants), an endemic area of American visceral leishmaniasis in the state of Minas Gerais, Brazil. A total number of 4795 dogs were examined by serology, which showed a rate of seropositivity of 5%. Isoenzymatic analysis confirmed Leishmania infantum chagasi as the local aetiological agent of CVL. Canine tissues were assayed for the presence of Leishmania parasite DNA using different techniques. The infectivity of asymptomatic, oligosymptomatic and symptomatic seropositive dogs was tested by xenodiagnosis using laboratory reared Lutzomyia longipalpis. Rates of infection of 5.4%, 5.1% and 28.4% were found for the phlebotomine sand flies that fed in asymptomatic, oligosymptomatic and symptomatic dogs, respectively. Our results indicate that, under experimental conditions, symptomatic dogs are about four times more infective to VL vectors than oligosymptomatic or asymptomatic animals. The lower infectivity rates of dogs displaying any of the last two clinical forms of leishmaniasis, however, must be taken into account in the epidemiology of CVL.  相似文献   

Evaluation of a novel nested PCR for the routine diagnosis of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV)     

Arjona A  Barquero N  Doménech A  Tejerizo G  Collado VM  Toural C  Martín D  Gomez-Lucia E 《Journal of Feline Medicine and Surgery》2007,9(1):14-22

Laboratory diagnosis of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) usually involves both viruses, as the clinical signs are similar and coinfection may occur. Serological methods may not represent an accurate diagnosis: maternal antibodies or cross-reactions may give false positive results to FIV, and false negative results may occur in latent FeLV status, or in certain FIV infection stages. A nested polymerase chain reaction (PCR) technique was designed to detect FeLV, FIV and feline endogenous retrovirus simultaneously. The detection of endogenous sequences was considered indicative of successful DNA extraction. The technique was used to diagnose FIV and FeLV in the blood cells of 179 cats. The kappa value with the serological data was 0.69 for FeLV and 0.87 for FIV. The joint detection of FeLV and FIV by this novel nested PCR is sensitive, specific, fast and convenient, and its applicability for clinical diagnosis is promising, as the direct evidence of the presence of the virus is more realistic than the indirect data provided by the serological detection.  相似文献   

Diagnostic needle arthroscopy of the tarsocrural joint in standing sedated horses     

Dimitri T. N. Kadic MVM  Alvaro G. Bonilla DVM  MSc  DACVS 《Veterinary surgery : VS》2020,49(3):445-454

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Needle arthroscopy of the radiocarpal and middle carpal joints in standing sedated horses     

Dimitri T. N. Kadic MVM  Ludovic Miagkoff DVM  Alvaro G. Bonilla DVM  MSc  DACVS 《Veterinary surgery : VS》2020,49(5):894-904

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Accelerating the development of new submergence tolerant rice varieties: the case of Ciherang-Sub1 and PSB Rc18-Sub1     

Endang M. Septiningsih  Nurul Hidayatun  Darlene L. Sanchez  Yudhistira Nugraha  Jerome Carandang  Alvaro M. Pamplona  Bertrand C. Y. Collard  Abdelbagi M. Ismail  David J. Mackill 《Euphytica》2015,202(2):259-268

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Creating an Earth Atmospheric Trust     

Barnes P  Costanza R  Hawken P  Orr D  Ostrom E  Umaña A  Young O 《Science (New York, N.Y.)》2008,319(5864):724

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