Ethyl p-methoxycinnamate isolated from a traditional anti-tuberculosis medicinal herb inhibits drug resistant strains of Mycobacterium tuberculosis in vitro     

Lakshmanan D  Werngren J  Jose L  Suja KP  Nair MS  Varma RL  Mundayoor S  Hoffner S  Kumar RA 《Fitoterapia》2011,82(5):757-761

Many plants are used in Ayurveda for the treatment of tuberculosis. Our aim was to examine if these plants possess any specific molecule that inhibits Mycobacterium tuberculosis. One of them, Kaempferia galanga, yielded an anti-TB molecule, ethyl p-methoxycinnamate (EPMC). By resazurin microtitre assay (REMA), EPMC was shown to inhibit M. tuberculosis H37Ra, H37Rv, drug susceptible and multidrug resistant (MDR) clinical isolates (MIC 0.242-0.485 mM). No cross resistance was observed to any standard anti-TB drugs in the MDR strains. The compound did not inhibit any prototype bacteria tested. EPMC seems to be a potential anti-TB lead molecule.  相似文献   

Analysis of differentially expressed transcripts in jute upon fungal infection and beta-amino butyric acid treatment     

Rudra Ray  Abhrajyoti Ghosh  Amit Bera  Nalok Dutta  D.J. Chattopadhyay  K. Chakrabarti 《Physiological and Molecular Plant Pathology》2011,76(1):59-66

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Relationship of light quantity and anthocyanin production in Pennisetum setaceum Cvs. rubrum and red riding hood     

Beckwith AG  Zhang Y  Seeram NP  Cameron AC  Nair MG 《Journal of agricultural and food chemistry》2004,52(3):456-461

Pennisetum setaceum cvs. Rubrum and Red Riding Hood are purple-pigmented ornamental grasses when grown in high-light environments. In low-light environments, foliage appears light purple or green, and as a result, aesthetic appeal is reduced. The impact of light on anthocyanin pigmentation was compared for P. setaceum Rubrum foliage and flowers and Red Riding Hood foliage grown under different light intensities and light sources. Light environments included UV supplemental light in the greenhouse, high-pressure sodium supplemental light in the greenhouse, cool-white fluorescent light in a growth chamber, and full sun outside. Anthocyanins in two cultivars of P. setaceum were analyzed by HPLC and characterized by (1)H and (13)C NMR spectral experiments. Two anthocyanins, cyanidin 3-glucoside and cyanidin 3-rutinoside, were identified in the leaves and flowers of both cultivars and quantified by HPLC analysis. The major anthocyanin in both cultivars was cyanidin 3-glucoside and had highest concentration (0.199% fresh weight) in Rubrum leaves grown under fluorescent lights in the growth chamber with a photoperiod of 24 h and a daily light integral (DLI) of 13.3 mol m(-)(2) day(-)(1) and in Rubrum and Red Riding Hood leaves and flowers (0.097 and 0.12% fresh weight) from plants grown outside in full sun with a photoperiod ranging from 15 to 13.5 h and DLI of 42 mol m(-)(2) day(-)(1). The minor anthocyanin, cyanidin 3-rutinoside, had the highest quantity in plants grown in low-light-intensity greenhouse environments with a photoperiod ranging from 15 to 13.5 h and DLI of 2.3-7.0 mol m(-)(2) day(-)(1). The functional significance of anthocyanins in P. setaceum Rubrum is discussed.  相似文献   

Bioactive compounds and 1,3-Di[(cis)-9-octadecenoyl]-2-[(cis,cis)-9, 12-octadecadienoyl]glycerol from Apium graveolens L. seeds     

Momin RA  Ramsewak RS  Nair MG 《Journal of agricultural and food chemistry》2000,48(9):3785-3788

Bioassay-directed isolation and purification of the hexane extract of Apium graveolens L. seeds led to the characterization of three compounds: beta-selinene (1), 3-n-butyl-4,5-dihydrophthalide (2) and 5-allyl-2-methoxyphenol (3). The structures of these compounds were established by using (1)H and (13)C NMR spectral methods. Compounds, 1-3 demonstrated 100% mortality on fourth-instar Aedes aegyptii larvae at 50, 25, and 200 microg mL(-)(1), respectively, in 24 h. Also, 2 inhibited the growth of Candida albicans and Candida kruseii at 100 microg mL(-)(1). It inhibited both topoisomerase-I and -II enzyme activities at 100 microg mL(-)(1). Compound 2 displayed 100% mortality at 12.5 and 50 microg mL(-)(1), respectively, when tested on nematodes, Panagrellus redivivus and Caenorhabditis elegans. The triglyceride, 1,3-di[(cis)-9-octadecenoyl]-2-[(cis,cis)-9, 12-octadecadienoyl]glycerol (4) and 3 were isolated for the first time from A. graveolens seeds, although 4 was not biologically active.  相似文献   

Relationship Between Leaf Nitrogen and Photosynthetic Characteristics in Brassica juncea and B. campestris     

K. Suresh  K. C. Lakkineni  T. V. R. Nair 《Journal of Agronomy and Crop Science》1996,177(2):107-113

The relationship between leaf nitrogen content (N) versus photosynthetic rate (PN) and other associated parameters was examined in Brassica juncea , cv. Pusa Bold and B campestris , cv. Pusa Kalyani. Leaf N, specific leaf weight (SLW), leaf area and PN were significantly higher in B. juncea , while the chlorophyll content was significantly lower compared to Brassica campestris. A significant positive correlation was obtained between leaf N content and photosynthetic rate in both species. Similarly, SLW was also positively related with leaf N content, Brassica juncea showed higher photosynthetic nitrogen use efficiency (PNUE) than B campestris. Leaf N and PNUE were negatively associated. This was attributed to a low investment of N in photosynthesis related reactions and/or partitioning of N towards compounds functionally unrelated to photosynthesis. This attribution is further supported by the negative relationship obtained between SLW and PNUE.  相似文献   

Leguminous Cover Crop Effects on Nitrogen Mineralization Rates and Kinetics in Soils     

R. Dinesh  M. A. Suryanarayana  A. K. Nair  & S. Ghoshal Chaudhuri 《Journal of Agronomy and Crop Science》2001,187(3):161-166

Soils were collected from an experimental site (initiated in 1991) at which leguminous crops were grown as natural soil cover in the interspaces of a 19‐year‐old coconut plantation. Atylosia scarabaeoides, Centrosema pubescens, Calopogonium mucunoides and Pueraria phaseoloides were grown in separate plots during the rainy season and ploughed into the soil towards the end of the monsoon (in December every year). Soil samples were collected from this site at the end of the 7th year and incubated in PVC columns at 35 ± 1°C and 0.01 MPa moisture content for 36 weeks. The soils were then leached at periodic intervals for up to 36 weeks and nitrogen (N) mineralization rates and kinetics were determined by the double exponential model. The N mineralization rates were highest during the first week and decreased with time in all soils. Soils amended (in situ ploughing) with cover crops leached 191 mg kg^–1 more NO₃^– + NO₂^–‐N than the unamended control. The per cent organic N mineralized (total and net) and the cumulative inorganic N mineralized (NO₃^– + NO₂^–‐N) varied with the amount (biomass) and type of cover crop incorporated into the soil. In general, soils amended with cover crops had greater N mineralization potentials and rate constants than the unamended control. The kinetic parameters N_oS and N_o(1 – S) and their respective rate constants h and k also varied with the amount and type of cover crops incorporated into the soil. The results further indicated that the lignin + polyphenol : N ratio of the cover crops is extremely important in predicting the rate of decomposition and N mineralization in soils.  相似文献   

Potential of ivermectin as an active ingredient of the attractive toxic sugar baits against the Indian malaria vectors Anopheles culicifacies and Anopheles stephensi     

Gaurav Kumar  Shweta Pasi  Chander Prakash Yadav  Jaspreet Kaur  Amit Sharma 《Pest management science》2023,79(1):474-480

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Effect of selected single nucleotide polymorphisms in SLC11A1, ANKRA2, IFNG and PGLYRP1 genes on host susceptibility to Mycobacterium avium subspecies paratuberculosis infection in Indian cattle     

Gopi  B.  Vir Singh  Ran  Kumar  Satish  Kumar  Sushil  Chauhan  Anuj  Sonwane  Arvind  Kumar  Amit  Bharati  Jaya  Vir Singh  Shoor 《Veterinary research communications》2022,46(1):209-221

Paratuberculosis (PTB) is a chronic infectious enteritis of ruminants, caused by Mycobacterium avium subspecies paratuberculosis (MAP) that brings huge economic loss to the dairy farmers. The study was conducted to explore the association of selected SNPs in IFNG, SLC11A1, ANKRA2 and PGLYRP1 genes with resistance to PTB disease in Indian cattle population. A case-control resource population was established based on the results of diagnostic tests used for detection of MAP infection status viz. ELISA, Johnin PPD test, faecal microscopy and IS900 blood PCR. The PCR-RFLP method was used for genotyping of SNPs. SNPs rs109453173 in SLC11A1, rs110853455 in IFNG and rs41933863 in ANKRA2 genes were significantly (P<0.05) associated with resistance to MAP infection. For SNP rs109453173, GG genotype and G allele was found to be associated with resistance against MAP infection than CC and CG genotypes and C allele, respectively. For SNP rs110853455, AG genotype was found to be associated with susceptibility to MAP infection than AA and GG genotype. For SNP rs41933863, the AG genotype provided three and six times more resistance against MAP infection than GG and AA genotype. The results of this study are suggestive of SNPs rs109453173, rs110853455 and rs41933863 as potential markers for screening MAP resistant cattle and a breeding programme favouring GG genotype and G allele for rs109453173, AG genotype for rs41933863 and against AG genotype for rs110853455 might confer resistance against MAP infection in Indian cattle. However, investigation of these SNPs in an independent and larger population will warrant the strength of association for resistance against MAP infection in cattle.

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L- -methyl- -hydrazino- -(3,4-dihydroxyphenyl)propionic acid: relative lack of antidecarboxylase activity in adrenals   总被引：1，自引：0，他引：1  

A Horita  X Nair  A E Hamilton 《Science (New York, N.Y.)》1972,176(37):931-932

In rats previously treated with a monoamine oxidase inhibitor, the administration of 5-hydroxytryptophan results in increases in concentrations of 5-hydroxytryptamine in kidney, brain, and adrenal glands. When the peripheral L-aromatic amino acid decarboxylase inhibitor, L-alpha-methyl-alpha-hydrazino-beta-(3,4-dihydroxyphenyl)propionic acid (HMD) is administered prior to 5-hydroxytryptophan, the concentration of 5-hydroxytryptamine in kidneys does not rise, that of the brain increases slightly, and that of the adrenal rises markedly. This indicates that although the adrenal gland is a peripheral organ, it does not respond in the typical manner to the antidecarboxylase action of HMD. These results suggest that HMD does not gain free access into the adrenal medulla and that a possible "blood-adrenal barrier" may exist to this compound.  相似文献   

Nutritional quality of the protein in quinoa (Chenopodium quinoa,Willd) seeds     

Jenny Ruales  Baboo M. Nair 《Plant foods for human nutrition (Dordrecht, Netherlands)》1992,42(1):1-11

The nutritional quality of protein in quinoa seeds has been determined by amino acid assay and by animal feeding experiments. The amino acid composition of the protein in raw quinoa and washed quinoa show similar pattern. The first limiting amino acids were the aromatic amino acids thyrosine + phenylalanine giving a chemical score of 86 for protein in raw quinoa and 85 for protein in washed quinoa. Threonine was the next limiting amino acid followed by lysine. The amount of lysine and sulfur amino acids (methionine + cystine) was relatively high. In general, the content of essential amino acids in quinoa is higher than in common cereals. The animal experiments showed NPU values of 75.7, BV of 82.6 and TD value of 91.7 for the protein in raw quinoa. Results of the in-vitro enzymatic methods showed that the digestibility of the protein in quinoa is comparable to that of other high quality food proteins. The corresponding experiments carried out with samples of guinoa seeds, which have been processed to remove the saponins, showed that, the saponins do not exert any negative effect on the nutritive quality of the protein.  相似文献